Q9WYH0 (PYRC_THEMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroorotase
Short name=DHOase
EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	TM_0335

Organism

Thermotoga maritima

Taxonomic identifier

2336 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate. HAMAP MF_00220_B
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_00220_B
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3. HAMAP MF_00220_B
Subunit structure	Homodimer By similarity. HAMAP MF_00220_B
Sequence similarities	Belongs to the DHOase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Dihydroorotase HAMAP MF_00220_B		PRO_0000147264
Regions
	Compositional bias	128 – 132	5	Poly-Lys HAMAP MF_00220_B
Sites
	Metal binding	35	1	Zinc 1 By similarity
	Metal binding	37	1	Zinc 1 By similarity
	Metal binding	138	1	Zinc 2 By similarity
	Metal binding	187	1	Zinc 2 By similarity
	Metal binding	257	1	Zinc 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9WYH0 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: C65EDF6EE0A95A81	FASTA	376	42,633
	10 20 30 40 50 60 MRIYDPFRKK WIEEEIETPF PSKGLVATFP FVDLHVHVRL NGGEDYSSLE EASLVGGFFK 70 80 90 100 110 120 VVVQPNTKPL IDSKEVLERH LDLSKNRAVE FLFAVSPFGS IEAEGERVVG FSTDGIEYDY 130 140 150 160 170 180 PTLVETMKKK KKALWFDHSQ MYEVDGIFYE GAPLPFQKRP RSNEAIAIAR TVLTGLEYGF 190 200 210 220 230 240 ERFHIQHVTT KYSVEVISFL KNLAKVSCEV TPHHLFFCYE DIKNTNFKIN PPLGSPEDRR 250 260 270 280 290 300 ALIEAVKKDV IDVLATDHAP HHEKPDDFLT APYGSTSIEI AFPAYYTALG DLELVVKKLT 310 320 330 340 350 360 KKPLEVLGVE ARLTEDTLVF IDPEAEFIVD AKKFKSKGKN SMFDGVRLKG KVVALKLKGR 370 WVMIDGEVIA DQKEND

References

[1]

"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima." Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Nature 399:323-329(1999) [PubMed: 10360571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000512 Genomic DNA. Translation: AAD35422.1.
PIR	A72391.
RefSeq	NP_228146.1. NC_000853.1.
3D structure databases
ProteinModelPortal	Q9WYH0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	897290.
GenomeReviews	Gene locus TM_0335 in contig AE000512_GR.
KEGG	tma:TM0335.
NMPDR	fig|243274.1.peg.330.
PATRIC	23935551. VBITheMar51294_0340.
TIGR	TM_0335.
Phylogenomic databases
HOGENOM	HBG724623.
OMA	GIFAEKE.
PhylomeDB	Q9WYH0.
ProtClustDB	PRK09357.
Enzyme and pathway databases
BioCyc	TMAR243274:TM_0335-MONOMER.
Family and domain databases
HAMAP	MF_00220_B. PyrC_type2_B. [Tree]
InterPro	IPR004722. DHOase. IPR002195. Dihydroorotase_CS. IPR011059. Metal-dep_hydrolase_composite. [Graphical view]
KO	K01465.
SUPFAM	SSF51338. Metalo_hydrolase. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_THEMA

Accession

Primary (citable) accession number: Q9WYH0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1999
Last modified:	January 25, 2012
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program