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Q9WYG7 (PYRF_THEMA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Orotidine 5'-phosphate decarboxylase
EC=4.1.1.23
Alternative name(s):
OMP decarboxylase
Short name=OMPDCase
Short name=OMPdecase
Gene names
Name:pyrF
Ordered Locus Names:TM_0332
OrganismThermotoga maritima
Taxonomic identifier2336 [NCBI]
Taxonomic lineageBacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length201 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the decarboxylation of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP) By similarity. HAMAP MF_01200_B

Catalytic activity

Orotidine 5'-phosphate = UMP + CO2. HAMAP MF_01200_B

Pathway

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2. HAMAP MF_01200_B

Subunit structure

Homodimer. Ref.2

Sequence similarities

Belongs to the OMP decarboxylase family. Type 1 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 201201Orotidine 5'-phosphate decarboxylase HAMAP MF_01200_B
PRO_0000134595

Regions

Region52 – 6110Substrate binding By similarity

Sites

Active site541Proton donor By similarity
Binding site81Substrate By similarity
Binding site261Substrate By similarity
Binding site1061Substrate By similarity
Binding site1531Substrate By similarity
Binding site1611Substrate By similarity
Binding site1801Substrate; via amide nitrogen By similarity
Binding site1811Substrate By similarity

Secondary structure

1 ......................................... 201
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q9WYG7 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 4C2FAD77302F206D

FASTA20122,809
        10         20         30         40         50         60 
MTPVLSLDME DPIRFIDENG SFEVVKVGHN LAIHGKKIFD ELAKRNLKII LDLKFCDIPS 

        70         80         90        100        110        120 
TVERSIKSWD HPAIIGFTVH SCAGYESVER ALSATDKHVF VVVKLTSMEG SLEDYMDRIE 

       130        140        150        160        170        180 
KLNKLGCDFV LPGPWAKALR EKIKGKILVP GIRMEVKADD QKDVVTLEEM KGIANFAVLG 

       190        200 
REIYLSENPR EKIKRIKEMR L

« Hide

References

« Hide 'large scale' references
[1]"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima."
Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. expand/collapse author list , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M.
Nature 399:323-329(1999) [PubMed: 10360571] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[2]"Crystal Structure of orotidine 5'-phosphate decarboxylase (TM0332) from Thermotoga maritima at 2.00 A resolution."
Joint center for structural genomics (JCSG)
Submitted (JAN-2005) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE000512 Genomic DNA. Translation: AAD35419.1.
PIRF72390.
RefSeqNP_228143.1. NC_000853.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1VQTX-ray2.00A1-201[»]
ProteinModelPortalQ9WYG7.
SMRQ9WYG7. Positions 1-198.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID897284.
GenomeReviewsGene locus TM_0332 in contig AE000512_GR.
KEGGtma:TM0332.
NMPDRfig|243274.1.peg.327.
PATRIC23935545. VBITheMar51294_0337.
TIGRTM_0332.

Phylogenomic databases

HOGENOMHBG476872.
OMAVILDLKF.
PhylomeDBQ9WYG7.
ProtClustDBCLSK875094.

Enzyme and pathway databases

BioCycTMAR243274:TM_0332-MONOMER.

Family and domain databases

HAMAPMF_01200_B. OMPdecase_type1_B. Divergent sequence.
[Tree]
InterProIPR013785. Aldolase_TIM.
IPR014732. OMPdecase.
IPR018089. OMPdecase_AS.
IPR001754. OMPdeCOase_dom.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KOK01591.
PfamPF00215. OMPdecase. 1 hit.
[Graphical view]
SMARTSM00934. OMPdecase. 1 hit.
[Graphical view]
SUPFAMSSF51366. RibP_bind_barrel. 1 hit.
TIGRFAMsTIGR01740. PyrF. 1 hit.
PROSITEPS00156. OMPDECASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePYRF_THEMA
AccessionPrimary (citable) accession number: Q9WYG7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: January 25, 2012
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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