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Protein

Glutamate 5-kinase

Gene

proB

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the transfer of a phosphate group to glutamate to form L-glutamate 5-phosphate.UniRule annotation

Catalytic activityi

ATP + L-glutamate = ADP + L-glutamate 5-phosphate.UniRule annotation

Pathwayi: L-proline biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate 5-kinase (proB), Glutamate 5-kinase (proB)
  2. Gamma-glutamyl phosphate reductase (proA), Gamma-glutamyl phosphate reductase (proA)
This subpathway is part of the pathway L-proline biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-glutamate 5-semialdehyde from L-glutamate, the pathway L-proline biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei8ATPUniRule annotation1
Binding sitei47SubstrateUniRule annotation1
Binding sitei134SubstrateUniRule annotation1
Binding sitei146Substrate; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi198 – 204ATPUniRule annotation7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Proline biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTMAR243274:G1H0Q-655-MONOMER
UniPathwayiUPA00098; UER00359

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate 5-kinaseUniRule annotation (EC:2.7.2.11UniRule annotation)
Alternative name(s):
Gamma-glutamyl kinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:proBUniRule annotation
Ordered Locus Names:TM_0294
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001097461 – 353Glutamate 5-kinaseAdd BLAST353

Proteomic databases

PRIDEiQ9WYD0

Interactioni

Protein-protein interaction databases

STRINGi243274.TM0294

Structurei

3D structure databases

ProteinModelPortaliQ9WYD0
SMRiQ9WYD0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini262 – 339PUAUniRule annotationAdd BLAST78

Sequence similaritiesi

Belongs to the glutamate 5-kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CGT Bacteria
COG0263 LUCA
InParanoidiQ9WYD0
KOiK00931
OMAiTHEIRFG

Family and domain databases

Gene3Di2.30.130.10, 1 hit
3.40.1160.10, 1 hit
HAMAPiMF_00456 ProB, 1 hit
InterProiView protein in InterPro
IPR036393 AceGlu_kinase-like_sf
IPR001048 Asp/Glu/Uridylate_kinase
IPR001057 Glu/AcGlu_kinase
IPR011529 Glu_5kinase
IPR005715 Glu_5kinase/COase_Synthase
IPR019797 Glutamate_5-kinase_CS
IPR002478 PUA
IPR015947 PUA-like_sf
IPR036974 PUA_sf
PfamiView protein in Pfam
PF00696 AA_kinase, 1 hit
PF01472 PUA, 1 hit
PIRSFiPIRSF000729 GK, 1 hit
PRINTSiPR00474 GLU5KINASE
SMARTiView protein in SMART
SM00359 PUA, 1 hit
SUPFAMiSSF53633 SSF53633, 1 hit
SSF88697 SSF88697, 1 hit
TIGRFAMsiTIGR01027 proB, 1 hit
PROSITEiView protein in PROSITE
PS00902 GLUTAMATE_5_KINASE, 1 hit
PS50890 PUA, 1 hit

Sequencei

Sequence statusi: Complete.

Q9WYD0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIMKVVVKVG SNLLVGSSGL RKSYIAELCR EVARLKSQGH EISIITSGAR
60 70 80 90 100
AAGFTYLGKG KRTQDLHIKQ ALCAVGQVQL MKVYENAFDF YGIKIAQILL
110 120 130 140 150
TRDTFSNRKR YLNLRNTLIG LSEFDVVPIV NENDTVATEE ITLGDNDTLA
160 170 180 190 200
AMFSIAWDAD FLVLFTTVDG VIDENGKLVE RFDESVKLKD MGKSSWGTGG
210 220 230 240 250
IRSKIESALM ASRCGVKATI CSGNDVSNLT RFVKGEPVGT VFEPQGRLKA
260 270 280 290 300
KKAWIAFLSE PAGKIYVNKG AEEALKSGNS LLPVGVTGVE GTFDVGDVVE
310 320 330 340 350
IVNEEGELVG RGIVNYSSSD LEKIAGHKSS DLKKILGYEG NKVVVHIDNM

WVA
Length:353
Mass (Da):38,320
Last modified:November 1, 1999 - v1
Checksum:i72E3171CBBB9F760
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA Translation: AAD35382.1
PIRiF72394
RefSeqiNP_228106.1, NC_000853.1
WP_010865089.1, NZ_CP011107.1

Genome annotation databases

EnsemblBacteriaiAAD35382; AAD35382; TM_0294
GeneIDi897219
KEGGitma:TM0294
PATRICifig|243274.5.peg.299

Similar proteinsi

Entry informationi

Entry nameiPROB_THEMA
AccessioniPrimary (citable) accession number: Q9WYD0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: November 1, 1999
Last modified: May 23, 2018
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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