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Q9WYD0 (PROB_THEMA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate 5-kinase

EC=2.7.2.11
Alternative name(s):
Gamma-glutamyl kinase
Short name=GK
Gene names
Name:proB
Ordered Locus Names:TM_0294
OrganismThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) [Reference proteome] [HAMAP]
Taxonomic identifier243274 [NCBI]
Taxonomic lineageBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga

Protein attributes

Sequence length353 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP-Rule MF_00456

Catalytic activity

ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP-Rule MF_00456

Pathway

Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP-Rule MF_00456

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00456.

Sequence similarities

Belongs to the glutamate 5-kinase family.

Contains 1 PUA domain.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Proline biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processL-proline biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA binding

Inferred from electronic annotation. Source: InterPro

glutamate 5-kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 353353Glutamate 5-kinase HAMAP-Rule MF_00456
PRO_0000109746

Regions

Domain262 – 33978PUA
Nucleotide binding198 – 2047ATP By similarity

Sites

Binding site81ATP By similarity
Binding site471Substrate By similarity
Binding site1341Substrate By similarity
Binding site1461Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9WYD0 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 72E3171CBBB9F760

FASTA35338,320
        10         20         30         40         50         60 
MIMKVVVKVG SNLLVGSSGL RKSYIAELCR EVARLKSQGH EISIITSGAR AAGFTYLGKG 

        70         80         90        100        110        120 
KRTQDLHIKQ ALCAVGQVQL MKVYENAFDF YGIKIAQILL TRDTFSNRKR YLNLRNTLIG 

       130        140        150        160        170        180 
LSEFDVVPIV NENDTVATEE ITLGDNDTLA AMFSIAWDAD FLVLFTTVDG VIDENGKLVE 

       190        200        210        220        230        240 
RFDESVKLKD MGKSSWGTGG IRSKIESALM ASRCGVKATI CSGNDVSNLT RFVKGEPVGT 

       250        260        270        280        290        300 
VFEPQGRLKA KKAWIAFLSE PAGKIYVNKG AEEALKSGNS LLPVGVTGVE GTFDVGDVVE 

       310        320        330        340        350 
IVNEEGELVG RGIVNYSSSD LEKIAGHKSS DLKKILGYEG NKVVVHIDNM WVA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000512 Genomic DNA. Translation: AAD35382.1.
PIRF72394.
RefSeqNP_228106.1. NC_000853.1.

3D structure databases

ProteinModelPortalQ9WYD0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243274.TM0294.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD35382; AAD35382; TM_0294.
GeneID897219.
KEGGtma:TM0294.
PATRIC23935467. VBITheMar51294_0299.

Phylogenomic databases

eggNOGCOG0263.
KOK00931.
OMAMRMIAGH.
OrthoDBEOG6PGK7G.

Enzyme and pathway databases

BioCycTMAR243274:GC6P-307-MONOMER.
UniPathwayUPA00098; UER00359.

Family and domain databases

Gene3D2.30.130.10. 1 hit.
3.40.1160.10. 1 hit.
HAMAPMF_00456. ProB.
InterProIPR001048. Asp/Glu/Uridylate_kinase.
IPR001057. Glu/AcGlu_kinase.
IPR011529. Glu_5kinase.
IPR005715. Glu_5kinase/COase_Synthase.
IPR019797. Glutamate_5-kinase_CS.
IPR002478. PUA.
IPR015947. PUA-like_domain.
[Graphical view]
PfamPF00696. AA_kinase. 1 hit.
PF01472. PUA. 1 hit.
[Graphical view]
PIRSFPIRSF000729. GK. 1 hit.
PRINTSPR00474. GLU5KINASE.
SMARTSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMSSF53633. SSF53633. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsTIGR01027. proB. 1 hit.
PROSITEPS00902. GLUTAMATE_5_KINASE. 1 hit.
PS50890. PUA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePROB_THEMA
AccessionPrimary (citable) accession number: Q9WYD0
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: November 1, 1999
Last modified: May 14, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways