Q9WYC8 (LEUD1_THEMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-isopropylmalate dehydratase small subunit 1
EC=4.2.1.33

Alternative name(s):
Alpha-IPM isomerase 1
Short name=IPMI 1
Isopropylmalate isomerase 1

Gene names

Name:	leuD1
Ordered Locus Names:	TM_0292

Organism

Thermotoga maritima

Taxonomic identifier

2336 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length	166 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate. HAMAP MF_01032
Catalytic activity	(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmaleate + H2O. HAMAP MF_01032 (2S)-2-isopropylmaleate + H2O = (2S)-2-isopropylmalate. HAMAP MF_01032
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4. HAMAP MF_01032
Subunit structure	Heterodimer of LeuC and LeuD By similarity. HAMAP MF_01032
Sequence similarities	Belongs to the LeuD family. LeuD type 2 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	leucine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	3-isopropylmalate dehydratase complex Inferred from electronic annotation. Source: InterPro
Molecular function	3-isopropylmalate dehydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 166	166	3-isopropylmalate dehydratase small subunit 1 HAMAP MF_01032		PRO_0000141927

Sequences

	Sequence		Length	Mass (Da)
	Q9WYC8 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: D8ACF340205111A4	FASTA	166	18,734
	10 20 30 40 50 60 MIRGRVWKFG DNISTDHIAP GRYFHLRNNL EELAKHVLED AMEDFAKKVQ KGDIIVAGKN 70 80 90 100 110 120 FGLGSSREHA ARIIKIAGVS CIVAKSFARI FYRNAINVGL PVIELKEVDE INQGDELEID 130 140 150 160 LENGVLKNLT TGKEYRFTPI PKFLLEILKE DGIVNYLKKH GSFPKV

References

[1]

"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima." Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Nature 399:323-329(1999) [PubMed: 10360571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000512 Genomic DNA. Translation: AAD35380.1.
PIR	D72394.
RefSeq	NP_228104.1. NC_000853.1.
3D structure databases
ProteinModelPortal	Q9WYC8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	897216.
GenomeReviews	Gene locus TM_0292 in contig AE000512_GR.
KEGG	tma:TM0292.
NMPDR	fig|243274.1.peg.288.
PATRIC	23935463. VBITheMar51294_0297.
TIGR	TM_0292.
Phylogenomic databases
HOGENOM	HBG304838.
OMA	DHAMEGI.
PhylomeDB	Q9WYC8.
ProtClustDB	PRK00439.
Enzyme and pathway databases
BioCyc	TMAR243274:TM_0292-MONOMER.
Family and domain databases
HAMAP	MF_01032. LeuD_type2. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR000573. AconitaseA/IPMdHydase_ssu_swvl. IPR011827. IsopropMal_deHydtase_ssu. [Graphical view]
Gene3D	G3DSA:3.20.19.10. Aconitase/3IPM_dehydase_swvl. 1 hit.
KO	K01704.
Pfam	PF00694. Aconitase_C. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR02087. LEUD_arch. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LEUD1_THEMA

Accession

Primary (citable) accession number: Q9WYC8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2002
Last sequence update:	November 1, 1999
Last modified:	January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program