Reviewed, UniProtKB/Swiss-Prot Q9WYB1 (ACKA_THEMA)

Names and origin

Protein names

Recommended name:
    Acetate kinase
    EC=2.7.2.1
Alternative name(s):
    Acetokinase

Gene names

Name:	ackA
Ordered Locus Names:	TM_0274

Organism

Thermotoga maritima [Complete proteome] [HAMAP]

Taxonomic identifier

2336 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Phosphorylates propionate (54%) in addition to acetate (100%). Uses GTP (100%), ITP (163%), UTP (56%), and CTP (21%) as phosphoryl donors in addition to ATP (100%). HAMAP MF_00020
Catalytic activity	ATP + acetate = ADP + acetyl phosphate. HAMAP MF_00020 ATP + propanoate = ADP + propanoyl phosphate. HAMAP MF_00020
Cofactor	Divalent cations. Manganese and magnesium are the most effective. HAMAP MF_00020
Pathway	Metabolic intermediate biosynthesis; acetyl-CoA biosynthesis; acetyl-CoA from acetate: step 1/2. HAMAP MF_00020
Subunit structure	Homodimer. HAMAP MF_00020
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the acetokinase family.
biophysicochemical properties	Temperature dependence: Optimum temperature is 90 degrees Celsius. HAMAP MF_00020

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	organic acid metabolic process Inferred from electronic annotation. Source: InterPro phosphorylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 403

403

Acetate kinase HAMAP MF_00020

PRO_0000107631

Secondary structure

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403

Helix Strand Turn

Details...

Beta strand

2 – 9

8

Beta strand

12 – 19

8

Turn

20 – 23

4

Beta strand

24 – 32

9

Beta strand

40 – 45

6

Beta strand

48 – 53

6

Helix

59 – 71

13

Turn

73 – 75

3

Beta strand

86 – 92

7

Turn

95 – 97

3

Helix

106 – 115

10

Helix

116 – 118

3

Turn

120 – 122

3

Helix

123 – 136

14

Beta strand

142 – 146

5

Helix

149 – 153

5

Helix

156 – 159

4

Helix

166 – 171

6

Helix

180 – 193

14

Helix

198 – 200

3

Beta strand

202 – 218

17

Beta strand

221 – 226

6

Beta strand

233 – 235

3

Helix

248 – 256

9

Helix

260 – 269

10

Helix

272 – 276

5

Turn

277 – 279

3

Helix

284 – 292

9

Helix

296 – 320

25

Beta strand

324 – 329

6

Helix

330 – 333

4

Helix

337 – 345

9

Helix

346 – 350

5

Helix

356 – 361

6

Beta strand

376 – 381

6

Helix

386 – 398

13

Sequences

	Sequence		Length	Mass (Da)
	Q9WYB1-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 98340ACB2AC04A7E	FASTA	403	44,804
	10 20 30 40 50 60 MRVLVINSGS SSIKYQLIEM EGEKVLCKGI AERIGIEGSR LVHRVGDEKH VIERELPDHE 70 80 90 100 110 120 EALKLILNTL VDEKLGVIKD LKEIDAVGHR VVHGGERFKE SVLVDEEVLK AIEEVSPLAP 130 140 150 160 170 180 LHNPANLMGI KAAMKLLPGV PNVAVFDTAF HQTIPQKAYL YAIPYEYYEK YKIRRYGFHG 190 200 210 220 230 240 TSHRYVSKRA AEILGKKLEE LKIITCHIGN GASVAAVKYG KCVDTSMGFT PLEGLVMGTR 250 260 270 280 290 300 SGDLDPAIPF FIMEKEGISP QEMYDILNKK SGVYGLSKGF SSDMRDIEEA ALKGDEWCKL 310 320 330 340 350 360 VLEIYDYRIA KYIGAYAAAM NGVDAIVFTA GVGENSPITR EDVCSYLEFL GVKLDKQKNE 370 380 390 400 ETIRGKEGII STPDSRVKVL VVPTNEELMI ARDTKEIVEK IGR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima." Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Nature 399:323-329(1999) [PubMed: 10360571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[2]	"Purification and characterization of two extremely thermostable enzymes, phosphate acetyltransferase and acetate kinase, from the hyperthermophilic eubacterium Thermotoga maritima." Bock A.-K., Glasemacher J., Schmidt R., Schoenheit P. J. Bacteriol. 181:1861-1867(1999) [PubMed: 10074080] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-12, CHARACTERIZATION.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AE000512 Genomic DNA. Translation: AAD35363.1.

PIR

H72397.

RefSeq

NP_228087.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2IIR	X-ray	3.30	A/B/C/D/E/F/G/H/I/J	1-403	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:2899N.

Genome annotation databases

GeneID

897189.

GenomeReviews

Gene locus TM_0274 in contig AE000512_GR.

KEGG

tma:TM0274.

NMPDR

fig|243274.1.peg.271.

TIGR

TM_0274.

Phylogenomic databases

HOGENOM

Q9WYB1.

OMA

Q9WYB1. SSDFRDV.

Enzyme and pathway databases

BioCyc

MetaCyc:MON-428.
TMAR243274:TM_0274-MON.

BRENDA

2.7.2.1. 16699.

Family and domain databases

HAMAP

MF_00020.
[Tree]

InterPro

IPR000890. Acetate_kin.
IPR004372. AckA.
[Graphical view]

PANTHER

PTHR21060. Acetate_kin. 1 hit.
PTHR21060:SF11. AckA. 1 hit.

Pfam

PF00871. Acetate_kinase. 1 hit.
[Graphical view]

PIRSF

PIRSF000722. Acetate_prop_kin. 1 hit.

PRINTS

PR00471. ACETATEKNASE.

TIGRFAMs

TIGR00016. ackA. 1 hit.

PROSITE

PS01075. ACETATE_KINASE_1. 1 hit.
PS01076. ACETATE_KINASE_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ACKA_THEMA

Accession

Primary (citable) accession number: Q9WYB1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1999
Last modified:	June 16, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)