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Protein

tRNA modification GTPase MnmE

Gene

mnmE

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34.

Cofactori

K+UniRule annotationNote: Binds 1 potassium ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei20FormyltetrahydrofolateUniRule annotation1 Publication1
Binding sitei78FormyltetrahydrofolateUniRule annotation1 Publication1
Binding sitei117FormyltetrahydrofolateUniRule annotation1 Publication1
Metal bindingi221PotassiumUniRule annotation1
Metal bindingi225MagnesiumUniRule annotation1
Metal bindingi240Potassium; via carbonyl oxygenUniRule annotation1
Metal bindingi242Potassium; via carbonyl oxygenUniRule annotation1
Metal bindingi245PotassiumUniRule annotation1
Metal bindingi246MagnesiumUniRule annotation1
Binding sitei450FormyltetrahydrofolateUniRule annotation1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi221 – 226GTPUniRule annotation6
Nucleotide bindingi240 – 246GTPUniRule annotation7
Nucleotide bindingi265 – 268GTPUniRule annotation4
Nucleotide bindingi326 – 329GTPUniRule annotation4
Nucleotide bindingi353 – 355GTPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA modification GTPase MnmEUniRule annotation (EC:3.6.5.-)
Gene namesi
Name:mnmEUniRule annotation
Synonyms:trmEUniRule annotation
Ordered Locus Names:TM_0267
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001889391 – 450tRNA modification GTPase MnmEAdd BLAST450

Interactioni

Subunit structurei

Homodimer. Heterotetramer of two MnmE and two MnmG subunits.UniRule annotation

Protein-protein interaction databases

STRINGi243274.TM0267.

Structurei

Secondary structure

1450
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 7Combined sources4
Beta strandi11 – 13Combined sources3
Beta strandi17 – 25Combined sources9
Helixi26 – 31Combined sources6
Beta strandi34 – 38Combined sources5
Beta strandi46 – 50Combined sources5
Beta strandi59 – 65Combined sources7
Beta strandi69 – 74Combined sources6
Beta strandi76 – 81Combined sources6
Helixi85 – 96Combined sources12
Turni97 – 99Combined sources3
Helixi107 – 114Combined sources8
Helixi120 – 131Combined sources12
Helixi135 – 145Combined sources11
Helixi148 – 172Combined sources25
Turni174 – 176Combined sources3
Helixi181 – 210Combined sources30
Beta strandi212 – 217Combined sources6
Helixi220 – 223Combined sources4
Helixi226 – 234Combined sources9
Beta strandi252 – 256Combined sources5
Beta strandi259 – 267Combined sources9
Helixi282 – 291Combined sources10
Beta strandi293 – 300Combined sources8
Helixi307 – 316Combined sources10
Beta strandi319 – 327Combined sources9
Helixi336 – 343Combined sources8
Beta strandi349 – 353Combined sources5
Helixi354 – 356Combined sources3
Helixi360 – 370Combined sources11
Helixi372 – 378Combined sources7
Helixi386 – 407Combined sources22
Helixi412 – 429Combined sources18
Beta strandi431 – 433Combined sources3
Helixi436 – 443Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XZPX-ray2.30A1-450[»]
B1-118[»]
1XZQX-ray2.90A1-450[»]
B1-117[»]
ProteinModelPortaliQ9WYA4.
SMRiQ9WYA4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WYA4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini211 – 372TrmE-type GAdd BLAST162

Sequence similaritiesi

Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C1H. Bacteria.
COG0486. LUCA.
InParanoidiQ9WYA4.
KOiK03650.
OMAiFTPRYAY.

Family and domain databases

CDDicd04164. trmE. 1 hit.
Gene3Di1.20.120.430. 3 hits.
3.30.1360.120. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00379. GTPase_MnmE. 1 hit.
InterProiIPR031168. G_TrmE.
IPR018948. GTP-bd_TrmE_N.
IPR006073. GTP_binding_domain.
IPR004520. GTPase_MnmE.
IPR008144. Guanylate_kin-like_dom.
IPR027368. MnmE_dom2.
IPR025867. MnmE_helical.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PfamiPF01926. MMR_HSR1. 1 hit.
PF12631. MnmE_helical. 1 hit.
PF10396. TrmE_N. 1 hit.
[Graphical view]
PRINTSiPR00326. GTP1OBG.
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00450. mnmE_trmE_thdF. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51709. G_TRME. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WYA4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTIVAVATP PGKGAIAILR LSGPDSWKIV QKHLRTRSKI VPRKAIHGWI
60 70 80 90 100
HENGEDVDEV VVVFYKSPKS YTGEDMVEVM CHGGPLVVKK LLDLFLKSGA
110 120 130 140 150
RMAEPGEFTK RAFLNGKMDL TSAEAVRDLI EAKSETSLKL SLRNLKGGLR
160 170 180 190 200
DFVDSLRREL IEVLAEIRVE LDYPDEIETN TGEVVTRLER IKEKLTEELK
210 220 230 240 250
KADAGILLNR GLRMVIVGKP NVGKSTLLNR LLNEDRAIVT DIPGTTRDVI
260 270 280 290 300
SEEIVIRGIL FRIVDTAGVR SETNDLVERL GIERTLQEIE KADIVLFVLD
310 320 330 340 350
ASSPLDEEDR KILERIKNKR YLVVINKVDV VEKINEEEIK NKLGTDRHMV
360 370 380 390 400
KISALKGEGL EKLEESIYRE TQEIFERGSD SLITNLRQKQ LLENVKGHLE
410 420 430 440 450
DAIKSLKEGM PVDMASIDLE RALNLLDEVT GRSFREDLLD TIFSNFCVGK
Length:450
Mass (Da):50,682
Last modified:November 1, 1999 - v1
Checksum:i8DAD2D1EB8500CC0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35356.1.
PIRiA72397.
RefSeqiNP_228080.1. NC_000853.1.
WP_004082971.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD35356; AAD35356; TM_0267.
GeneIDi897178.
KEGGitma:TM0267.
PATRICi23935411. VBITheMar51294_0271.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35356.1.
PIRiA72397.
RefSeqiNP_228080.1. NC_000853.1.
WP_004082971.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XZPX-ray2.30A1-450[»]
B1-118[»]
1XZQX-ray2.90A1-450[»]
B1-117[»]
ProteinModelPortaliQ9WYA4.
SMRiQ9WYA4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM0267.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD35356; AAD35356; TM_0267.
GeneIDi897178.
KEGGitma:TM0267.
PATRICi23935411. VBITheMar51294_0271.

Phylogenomic databases

eggNOGiENOG4105C1H. Bacteria.
COG0486. LUCA.
InParanoidiQ9WYA4.
KOiK03650.
OMAiFTPRYAY.

Miscellaneous databases

EvolutionaryTraceiQ9WYA4.

Family and domain databases

CDDicd04164. trmE. 1 hit.
Gene3Di1.20.120.430. 3 hits.
3.30.1360.120. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00379. GTPase_MnmE. 1 hit.
InterProiIPR031168. G_TrmE.
IPR018948. GTP-bd_TrmE_N.
IPR006073. GTP_binding_domain.
IPR004520. GTPase_MnmE.
IPR008144. Guanylate_kin-like_dom.
IPR027368. MnmE_dom2.
IPR025867. MnmE_helical.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PfamiPF01926. MMR_HSR1. 1 hit.
PF12631. MnmE_helical. 1 hit.
PF10396. TrmE_N. 1 hit.
[Graphical view]
PRINTSiPR00326. GTP1OBG.
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00450. mnmE_trmE_thdF. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51709. G_TRME. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMNME_THEMA
AccessioniPrimary (citable) accession number: Q9WYA4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: November 1, 1999
Last modified: November 2, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.