Reviewed, UniProtKB/Swiss-Prot Q9WYA4 (MNME_THEMA)

Names and origin

Protein names

Recommended name:
    tRNA modification GTPase mnmE
    EC=3.6.5.-

Gene names

Name:	mnmE
Synonyms:	trmE
Ordered Locus Names:	TM_0267

Organism

Thermotoga maritima [Complete proteome] [HAMAP]

Taxonomic identifier

2336 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34. HAMAP MF_00379
Cofactor	Binds 1 potassium ion per subunit By similarity.
Subunit structure	Homodimer. Heterotetramer of two mnmE and two mnmG subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the era/mnmE GTP-binding protein family. MnmE subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding Potassium
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: HAMAP GTPase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 450

450

tRNA modification GTPase mnmE HAMAP MF_00379

PRO_0000188939

Regions

Nucleotide binding

221 – 226

6

GTP By similarity

Nucleotide binding

240 – 246

7

GTP By similarity

Nucleotide binding

265 – 268

4

GTP By similarity

Nucleotide binding

326 – 329

4

GTP By similarity

Nucleotide binding

353 – 355

3

GTP By similarity

Sites

Metal binding

221

1

Potassium By similarity

Metal binding

225

1

Magnesium By similarity

Metal binding

240

1

Potassium; via carbonyl oxygen By similarity

Metal binding

242

1

Potassium; via carbonyl oxygen By similarity

Metal binding

245

1

Potassium By similarity

Metal binding

246

1

Magnesium By similarity

Binding site

20

1

Formyltetrahydrofolate HAMAP MF_00379

Binding site

78

1

Formyltetrahydrofolate HAMAP MF_00379

Binding site

117

1

Formyltetrahydrofolate HAMAP MF_00379

Binding site

450

1

Formyltetrahydrofolate HAMAP MF_00379

Secondary structure

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450

Helix Strand Turn

Details...

Beta strand

4 – 7

4

Beta strand

11 – 13

3

Beta strand

17 – 25

9

Helix

26 – 31

6

Beta strand

34 – 38

5

Beta strand

46 – 50

5

Beta strand

59 – 65

7

Beta strand

69 – 74

6

Beta strand

76 – 81

6

Helix

85 – 96

12

Turn

97 – 99

3

Helix

107 – 114

8

Helix

120 – 131

12

Helix

135 – 145

11

Helix

148 – 172

25

Turn

174 – 176

3

Helix

181 – 210

30

Beta strand

212 – 217

6

Helix

220 – 223

4

Helix

226 – 234

9

Beta strand

252 – 256

5

Beta strand

259 – 267

9

Helix

282 – 291

10

Beta strand

293 – 300

8

Helix

307 – 316

10

Beta strand

319 – 327

9

Helix

336 – 343

8

Beta strand

349 – 353

5

Helix

354 – 356

3

Helix

360 – 370

11

Helix

372 – 378

7

Helix

386 – 407

22

Helix

412 – 429

18

Helix

436 – 443

8

Sequences

	Sequence		Length	Mass (Da)
	Q9WYA4-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 8DAD2D1EB8500CC0	FASTA	450	50,682
	10 20 30 40 50 60 MDTIVAVATP PGKGAIAILR LSGPDSWKIV QKHLRTRSKI VPRKAIHGWI HENGEDVDEV 70 80 90 100 110 120 VVVFYKSPKS YTGEDMVEVM CHGGPLVVKK LLDLFLKSGA RMAEPGEFTK RAFLNGKMDL 130 140 150 160 170 180 TSAEAVRDLI EAKSETSLKL SLRNLKGGLR DFVDSLRREL IEVLAEIRVE LDYPDEIETN 190 200 210 220 230 240 TGEVVTRLER IKEKLTEELK KADAGILLNR GLRMVIVGKP NVGKSTLLNR LLNEDRAIVT 250 260 270 280 290 300 DIPGTTRDVI SEEIVIRGIL FRIVDTAGVR SETNDLVERL GIERTLQEIE KADIVLFVLD 310 320 330 340 350 360 ASSPLDEEDR KILERIKNKR YLVVINKVDV VEKINEEEIK NKLGTDRHMV KISALKGEGL 370 380 390 400 410 420 EKLEESIYRE TQEIFERGSD SLITNLRQKQ LLENVKGHLE DAIKSLKEGM PVDMASIDLE 430 440 450 RALNLLDEVT GRSFREDLLD TIFSNFCVGK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima." Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Nature 399:323-329(1999) [PubMed: 10360571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[2]	"Characterization of GTPase activity of TrmE, a member of a novel GTPase superfamily, from Thermotoga maritima." Yamanaka K., Hwang J., Inouye M. J. Bacteriol. 182:7078-7082(2000) [PubMed: 11092873] [Abstract] Cited for: CHARACTERIZATION.
[3]	"The structure of the TrmE GTP-binding protein and its implications for tRNA modification." Scrima A., Vetter I.R., Armengod M.-E., Wittinghofer A. EMBO J. 24:23-33(2005) [PubMed: 15616586] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) IN COMPLEX WITH FORMYLTETRAHYDROFOLATE; GTP-BINDING, SUBUNIT.

Cross-references

Sequence databases

EMBLGenBankDDBJ

AE000512 Genomic DNA. Translation: AAD35356.1.

PIR

A72397.

RefSeq

NP_228080.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1XZP	X-ray	2.30	A	1-450	[»]
			B	1-118	[»]
1XZQ	X-ray	2.90	A	1-450	[»]
			B	1-117	[»]

ModBase

Search...

Genome annotation databases

GeneID

897178.

GenomeReviews

Gene locus TM_0267 in contig AE000512_GR.

KEGG

tma:TM0267.

NMPDR

fig|243274.1.peg.264.

TIGR

TM_0267.

Phylogenomic databases

HOGENOM

Q9WYA4.

OMA

KGPNSFT.

Enzyme and pathway databases

BioCyc

TMAR243274:TM_0267-MON.

Family and domain databases

HAMAP

MF_00379.
[Tree]

InterPro

IPR006073. GTP1_OBG.
IPR018948. GTP_bd_TrmE_N.
IPR002917. MMR_HSR1_GTP_bd.
IPR005225. Small_GTP_bd.
IPR004520. ThdF.
[Graphical view]

Gene3D

G3DSA:3.30.1360.120. GTP_bd_TrmE_N. 1 hit.

Pfam

PF01926. MMR_HSR1. 1 hit.
PF10396. TrmE_N. 1 hit.
[Graphical view]

PRINTS

PR00326. GTP1OBG.

TIGRFAMs

TIGR00450. mnmE_trmE_thdF. 1 hit.
TIGR00231. small_GTP. 1 hit.

ProtoNet

Search...

Entry information

Entry name

MNME_THEMA

Accession

Primary (citable) accession number: Q9WYA4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	November 1, 1999
Last modified:	November 3, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)