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Protein

tRNA modification GTPase MnmE

Gene

mnmE

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34.

Cofactori

K+UniRule annotationNote: Binds 1 potassium ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei20FormyltetrahydrofolateUniRule annotation1 Publication1
Binding sitei78FormyltetrahydrofolateUniRule annotation1 Publication1
Binding sitei117FormyltetrahydrofolateUniRule annotation1 Publication1
Metal bindingi221PotassiumUniRule annotation1
Metal bindingi225MagnesiumUniRule annotation1
Metal bindingi240Potassium; via carbonyl oxygenUniRule annotation1
Metal bindingi242Potassium; via carbonyl oxygenUniRule annotation1
Metal bindingi245PotassiumUniRule annotation1
Metal bindingi246MagnesiumUniRule annotation1
Binding sitei450FormyltetrahydrofolateUniRule annotation1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi221 – 226GTPUniRule annotation6
Nucleotide bindingi240 – 246GTPUniRule annotation7
Nucleotide bindingi265 – 268GTPUniRule annotation4
Nucleotide bindingi326 – 329GTPUniRule annotation4
Nucleotide bindingi353 – 355GTPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processtRNA processing
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA modification GTPase MnmEUniRule annotation (EC:3.6.5.-)
Gene namesi
Name:mnmEUniRule annotation
Synonyms:trmEUniRule annotation
Ordered Locus Names:TM_0267
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001889391 – 450tRNA modification GTPase MnmEAdd BLAST450

Interactioni

Subunit structurei

Homodimer. Heterotetramer of two MnmE and two MnmG subunits.UniRule annotation

Protein-protein interaction databases

STRINGi243274.TM0267

Structurei

Secondary structure

1450
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 7Combined sources4
Beta strandi11 – 13Combined sources3
Beta strandi17 – 25Combined sources9
Helixi26 – 31Combined sources6
Beta strandi34 – 38Combined sources5
Beta strandi46 – 50Combined sources5
Beta strandi59 – 65Combined sources7
Beta strandi69 – 74Combined sources6
Beta strandi76 – 81Combined sources6
Helixi85 – 96Combined sources12
Turni97 – 99Combined sources3
Helixi107 – 114Combined sources8
Helixi120 – 131Combined sources12
Helixi135 – 145Combined sources11
Helixi148 – 172Combined sources25
Turni174 – 176Combined sources3
Helixi181 – 210Combined sources30
Beta strandi212 – 217Combined sources6
Helixi220 – 223Combined sources4
Helixi226 – 234Combined sources9
Beta strandi252 – 256Combined sources5
Beta strandi259 – 267Combined sources9
Helixi282 – 291Combined sources10
Beta strandi293 – 300Combined sources8
Helixi307 – 316Combined sources10
Beta strandi319 – 327Combined sources9
Helixi336 – 343Combined sources8
Beta strandi349 – 353Combined sources5
Helixi354 – 356Combined sources3
Helixi360 – 370Combined sources11
Helixi372 – 378Combined sources7
Helixi386 – 407Combined sources22
Helixi412 – 429Combined sources18
Beta strandi431 – 433Combined sources3
Helixi436 – 443Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XZPX-ray2.30A1-450[»]
B1-118[»]
1XZQX-ray2.90A1-450[»]
B1-117[»]
ProteinModelPortaliQ9WYA4
SMRiQ9WYA4
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WYA4

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini211 – 372TrmE-type GAdd BLAST162

Sequence similaritiesi

Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C1H Bacteria
COG0486 LUCA
InParanoidiQ9WYA4
KOiK03650
OMAiEFTQRAF

Family and domain databases

CDDicd04164 trmE, 1 hit
Gene3Di1.20.120.430, 2 hits
3.30.1360.120, 1 hit
HAMAPiMF_00379 GTPase_MnmE, 1 hit
InterProiView protein in InterPro
IPR031168 G_TrmE
IPR018948 GTP-bd_TrmE_N
IPR006073 GTP_binding_domain
IPR004520 GTPase_MnmE
IPR008144 Guanylate_kin-like_dom
IPR027368 MnmE_dom2
IPR025867 MnmE_helical
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR027266 TrmE/GcvT_dom1
PfamiView protein in Pfam
PF01926 MMR_HSR1, 1 hit
PF12631 MnmE_helical, 1 hit
PF10396 TrmE_N, 1 hit
PRINTSiPR00326 GTP1OBG
SUPFAMiSSF52540 SSF52540, 2 hits
TIGRFAMsiTIGR00450 mnmE_trmE_thdF, 1 hit
TIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51709 G_TRME, 1 hit

Sequencei

Sequence statusi: Complete.

Q9WYA4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTIVAVATP PGKGAIAILR LSGPDSWKIV QKHLRTRSKI VPRKAIHGWI
60 70 80 90 100
HENGEDVDEV VVVFYKSPKS YTGEDMVEVM CHGGPLVVKK LLDLFLKSGA
110 120 130 140 150
RMAEPGEFTK RAFLNGKMDL TSAEAVRDLI EAKSETSLKL SLRNLKGGLR
160 170 180 190 200
DFVDSLRREL IEVLAEIRVE LDYPDEIETN TGEVVTRLER IKEKLTEELK
210 220 230 240 250
KADAGILLNR GLRMVIVGKP NVGKSTLLNR LLNEDRAIVT DIPGTTRDVI
260 270 280 290 300
SEEIVIRGIL FRIVDTAGVR SETNDLVERL GIERTLQEIE KADIVLFVLD
310 320 330 340 350
ASSPLDEEDR KILERIKNKR YLVVINKVDV VEKINEEEIK NKLGTDRHMV
360 370 380 390 400
KISALKGEGL EKLEESIYRE TQEIFERGSD SLITNLRQKQ LLENVKGHLE
410 420 430 440 450
DAIKSLKEGM PVDMASIDLE RALNLLDEVT GRSFREDLLD TIFSNFCVGK
Length:450
Mass (Da):50,682
Last modified:November 1, 1999 - v1
Checksum:i8DAD2D1EB8500CC0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA Translation: AAD35356.1
PIRiA72397
RefSeqiNP_228080.1, NC_000853.1
WP_004082971.1, NZ_CP011107.1

Genome annotation databases

EnsemblBacteriaiAAD35356; AAD35356; TM_0267
GeneIDi897178
KEGGitma:TM0267

Similar proteinsi

Entry informationi

Entry nameiMNME_THEMA
AccessioniPrimary (citable) accession number: Q9WYA4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: November 1, 1999
Last modified: March 28, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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