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Protein

tRNA modification GTPase MnmE

Gene

mnmE

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34.

Cofactori

K+UniRule annotationNote: Binds 1 potassium ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei20 – 201FormyltetrahydrofolateUniRule annotation1 Publication
Binding sitei78 – 781FormyltetrahydrofolateUniRule annotation1 Publication
Binding sitei117 – 1171FormyltetrahydrofolateUniRule annotation1 Publication
Metal bindingi221 – 2211PotassiumUniRule annotation
Metal bindingi225 – 2251MagnesiumUniRule annotation
Metal bindingi240 – 2401Potassium; via carbonyl oxygenUniRule annotation
Metal bindingi242 – 2421Potassium; via carbonyl oxygenUniRule annotation
Metal bindingi245 – 2451PotassiumUniRule annotation
Metal bindingi246 – 2461MagnesiumUniRule annotation
Binding sitei450 – 4501FormyltetrahydrofolateUniRule annotation1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi221 – 2266GTPUniRule annotation
Nucleotide bindingi240 – 2467GTPUniRule annotation
Nucleotide bindingi265 – 2684GTPUniRule annotation
Nucleotide bindingi326 – 3294GTPUniRule annotation
Nucleotide bindingi353 – 3553GTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA modification GTPase MnmEUniRule annotation (EC:3.6.5.-)
Gene namesi
Name:mnmEUniRule annotation
Synonyms:trmEUniRule annotation
Ordered Locus Names:TM_0267
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 450450tRNA modification GTPase MnmEPRO_0000188939Add
BLAST

Interactioni

Subunit structurei

Homodimer. Heterotetramer of two MnmE and two MnmG subunits.UniRule annotation

Protein-protein interaction databases

STRINGi243274.TM0267.

Structurei

Secondary structure

1
450
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 74Combined sources
Beta strandi11 – 133Combined sources
Beta strandi17 – 259Combined sources
Helixi26 – 316Combined sources
Beta strandi34 – 385Combined sources
Beta strandi46 – 505Combined sources
Beta strandi59 – 657Combined sources
Beta strandi69 – 746Combined sources
Beta strandi76 – 816Combined sources
Helixi85 – 9612Combined sources
Turni97 – 993Combined sources
Helixi107 – 1148Combined sources
Helixi120 – 13112Combined sources
Helixi135 – 14511Combined sources
Helixi148 – 17225Combined sources
Turni174 – 1763Combined sources
Helixi181 – 21030Combined sources
Beta strandi212 – 2176Combined sources
Helixi220 – 2234Combined sources
Helixi226 – 2349Combined sources
Beta strandi252 – 2565Combined sources
Beta strandi259 – 2679Combined sources
Helixi282 – 29110Combined sources
Beta strandi293 – 3008Combined sources
Helixi307 – 31610Combined sources
Beta strandi319 – 3279Combined sources
Helixi336 – 3438Combined sources
Beta strandi349 – 3535Combined sources
Helixi354 – 3563Combined sources
Helixi360 – 37011Combined sources
Helixi372 – 3787Combined sources
Helixi386 – 40722Combined sources
Helixi412 – 42918Combined sources
Beta strandi431 – 4333Combined sources
Helixi436 – 4438Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1XZPX-ray2.30A1-450[»]
B1-118[»]
1XZQX-ray2.90A1-450[»]
B1-117[»]
ProteinModelPortaliQ9WYA4.
SMRiQ9WYA4. Positions 1-450.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WYA4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini211 – 372162TrmE-type GAdd
BLAST

Sequence similaritiesi

Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C1H. Bacteria.
COG0486. LUCA.
InParanoidiQ9WYA4.
KOiK03650.
OMAiFTPRYAY.
OrthoDBiEOG6DC6K1.

Family and domain databases

Gene3Di1.20.120.430. 3 hits.
3.30.1360.120. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00379. GTPase_MnmE.
InterProiIPR031168. G_TrmE.
IPR018948. GTP-bd_TrmE_N.
IPR006073. GTP_binding_domain.
IPR004520. GTPase_MnmE.
IPR008144. Guanylate_kin-like_dom.
IPR027368. MnmE_dom2.
IPR025867. MnmE_helical.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PfamiPF01926. MMR_HSR1. 1 hit.
PF12631. MnmE_helical. 1 hit.
PF10396. TrmE_N. 1 hit.
[Graphical view]
PRINTSiPR00326. GTP1OBG.
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00450. mnmE_trmE_thdF. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51709. G_TRME. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WYA4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTIVAVATP PGKGAIAILR LSGPDSWKIV QKHLRTRSKI VPRKAIHGWI
60 70 80 90 100
HENGEDVDEV VVVFYKSPKS YTGEDMVEVM CHGGPLVVKK LLDLFLKSGA
110 120 130 140 150
RMAEPGEFTK RAFLNGKMDL TSAEAVRDLI EAKSETSLKL SLRNLKGGLR
160 170 180 190 200
DFVDSLRREL IEVLAEIRVE LDYPDEIETN TGEVVTRLER IKEKLTEELK
210 220 230 240 250
KADAGILLNR GLRMVIVGKP NVGKSTLLNR LLNEDRAIVT DIPGTTRDVI
260 270 280 290 300
SEEIVIRGIL FRIVDTAGVR SETNDLVERL GIERTLQEIE KADIVLFVLD
310 320 330 340 350
ASSPLDEEDR KILERIKNKR YLVVINKVDV VEKINEEEIK NKLGTDRHMV
360 370 380 390 400
KISALKGEGL EKLEESIYRE TQEIFERGSD SLITNLRQKQ LLENVKGHLE
410 420 430 440 450
DAIKSLKEGM PVDMASIDLE RALNLLDEVT GRSFREDLLD TIFSNFCVGK
Length:450
Mass (Da):50,682
Last modified:November 1, 1999 - v1
Checksum:i8DAD2D1EB8500CC0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35356.1.
PIRiA72397.
RefSeqiNP_228080.1. NC_000853.1.
WP_004082971.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD35356; AAD35356; TM_0267.
GeneIDi897178.
KEGGitma:TM0267.
PATRICi23935411. VBITheMar51294_0271.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35356.1.
PIRiA72397.
RefSeqiNP_228080.1. NC_000853.1.
WP_004082971.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1XZPX-ray2.30A1-450[»]
B1-118[»]
1XZQX-ray2.90A1-450[»]
B1-117[»]
ProteinModelPortaliQ9WYA4.
SMRiQ9WYA4. Positions 1-450.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM0267.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD35356; AAD35356; TM_0267.
GeneIDi897178.
KEGGitma:TM0267.
PATRICi23935411. VBITheMar51294_0271.

Phylogenomic databases

eggNOGiENOG4105C1H. Bacteria.
COG0486. LUCA.
InParanoidiQ9WYA4.
KOiK03650.
OMAiFTPRYAY.
OrthoDBiEOG6DC6K1.

Miscellaneous databases

EvolutionaryTraceiQ9WYA4.

Family and domain databases

Gene3Di1.20.120.430. 3 hits.
3.30.1360.120. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00379. GTPase_MnmE.
InterProiIPR031168. G_TrmE.
IPR018948. GTP-bd_TrmE_N.
IPR006073. GTP_binding_domain.
IPR004520. GTPase_MnmE.
IPR008144. Guanylate_kin-like_dom.
IPR027368. MnmE_dom2.
IPR025867. MnmE_helical.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR027266. TrmE/GcvT_dom1.
[Graphical view]
PfamiPF01926. MMR_HSR1. 1 hit.
PF12631. MnmE_helical. 1 hit.
PF10396. TrmE_N. 1 hit.
[Graphical view]
PRINTSiPR00326. GTP1OBG.
SUPFAMiSSF52540. SSF52540. 2 hits.
TIGRFAMsiTIGR00450. mnmE_trmE_thdF. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS51709. G_TRME. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
  2. "Characterization of GTPase activity of TrmE, a member of a novel GTPase superfamily, from Thermotoga maritima."
    Yamanaka K., Hwang J., Inouye M.
    J. Bacteriol. 182:7078-7082(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  3. "The structure of the TrmE GTP-binding protein and its implications for tRNA modification."
    Scrima A., Vetter I.R., Armengod M.-E., Wittinghofer A.
    EMBO J. 24:23-33(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) IN COMPLEX WITH FORMYLTETRAHYDROFOLATE; GTP-BINDING, SUBUNIT.

Entry informationi

Entry nameiMNME_THEMA
AccessioniPrimary (citable) accession number: Q9WYA4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: November 1, 1999
Last modified: April 13, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.