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Q9WY65

- ECFA2_THEMA

UniProt

Q9WY65 - ECFA2_THEMA

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Protein

Energy-coupling factor transporter ATP-binding protein EcfA2

Gene

ecfA2

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

ATP-binding (A) component of a common energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates (Probable). Expression of the complex plus RibU in E.coli allows riboflavin uptake; uptake does not occur in the absence of RibU or the EcfA1A2T complex.1 PublicationCurated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei164 – 1641Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi43 – 486ATP

GO - Molecular functioni

  1. ATPase activity Source: InterPro
  2. ATP binding Source: UniProtKB
  3. riboflavin transporter activity Source: UniProtKB

GO - Biological processi

  1. riboflavin transport Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Protein family/group databases

TCDBi3.A.1.25.5. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Energy-coupling factor transporter ATP-binding protein EcfA2 (EC:3.6.3.-)
Short name:
ECF transporter A component EcfA2
Gene namesi
Name:ecfA2
Synonyms:ecfA, ecfA'
Ordered Locus Names:TM_0222
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
ProteomesiUP000008183: Chromosome

Subcellular locationi

Cell inner membrane 1 Publication; Peripheral membrane protein 1 Publication

GO - Cellular componenti

  1. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 266266Energy-coupling factor transporter ATP-binding protein EcfA2PRO_0000092115Add
BLAST

Interactioni

Subunit structurei

Forms a heterodimer with EcfA1. Forms a stable energy-coupling factor (ECF) transporter complex composed of 2 membrane-embedded substrate-binding proteins (S component, RibU, BioY), 2 ATP-binding proteins (A component) and 2 transmembrane proteins (T component) upon coexpression in E.coli. Stable subcomplexes with both A plus T components can also be isolated. This complex interacts with at least 2 substrate-specific components, BioY and RibU.1 Publication

Protein-protein interaction databases

STRINGi243274.TM0222.

Structurei

Secondary structure

1
266
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 1311Combined sources
Beta strandi20 – 3011Combined sources
Beta strandi35 – 395Combined sources
Helixi46 – 538Combined sources
Beta strandi60 – 667Combined sources
Helixi73 – 764Combined sources
Helixi77 – 793Combined sources
Beta strandi80 – 834Combined sources
Helixi87 – 904Combined sources
Helixi96 – 1027Combined sources
Turni103 – 1075Combined sources
Helixi114 – 12310Combined sources
Helixi128 – 1314Combined sources
Helixi136 – 1383Combined sources
Helixi141 – 15313Combined sources
Beta strandi158 – 1647Combined sources
Turni165 – 1684Combined sources
Helixi171 – 18616Combined sources
Beta strandi190 – 1945Combined sources
Turni199 – 2013Combined sources
Helixi202 – 2043Combined sources
Beta strandi206 – 2127Combined sources
Beta strandi215 – 2217Combined sources
Helixi222 – 2287Combined sources
Helixi231 – 2333Combined sources
Helixi236 – 2438Combined sources
Turni244 – 2474Combined sources
Beta strandi252 – 2543Combined sources
Helixi257 – 2604Combined sources
Turni261 – 2633Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2YZ2X-ray2.30A/B1-266[»]
4HLUX-ray2.70A/B2-266[»]
ProteinModelPortaliQ9WY65.
SMRiQ9WY65. Positions 1-266.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WY65.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 238236ABC transporterPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni220 – 26647Required for heterodimer formationAdd
BLAST

Sequence similaritiesi

Contains 1 ABC transporter domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG1122.
InParanoidiQ9WY65.
KOiK16787.
OMAiEMVARET.
OrthoDBiEOG6T7N3V.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WY65-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRIEVVNVSH IFHRGTPLEK KALENVSLVI NEGECLLVAG NTGSGKSTLL
60 70 80 90 100
QIVAGLIEPT SGDVLYDGER KKGYEIRRNI GIAFQYPEDQ FFAERVFDEV
110 120 130 140 150
AFAVKNFYPD RDPVPLVKKA MEFVGLDFDS FKDRVPFFLS GGEKRRVAIA
160 170 180 190 200
SVIVHEPDIL ILDEPLVGLD REGKTDLLRI VEKWKTLGKT VILISHDIET
210 220 230 240 250
VINHVDRVVV LEKGKKVFDG TRMEFLEKYD PRFFTSKMLV MRRLVLKGED
260
PFSMSDDELL ERVCNS
Length:266
Mass (Da):30,233
Last modified:November 1, 1999 - v1
Checksum:iEB146157FC21EB4F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35314.1.
PIRiA72401.
RefSeqiNP_228037.1. NC_000853.1.
YP_007976571.1. NC_021214.1.
YP_008990804.1. NC_023151.1.

Genome annotation databases

EnsemblBacteriaiAAD35314; AAD35314; TM_0222.
GeneIDi18092577.
897116.
KEGGitma:TM0222.
PATRICi23935316. VBITheMar51294_0224.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35314.1 .
PIRi A72401.
RefSeqi NP_228037.1. NC_000853.1.
YP_007976571.1. NC_021214.1.
YP_008990804.1. NC_023151.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2YZ2 X-ray 2.30 A/B 1-266 [» ]
4HLU X-ray 2.70 A/B 2-266 [» ]
ProteinModelPortali Q9WY65.
SMRi Q9WY65. Positions 1-266.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243274.TM0222.

Protein family/group databases

TCDBi 3.A.1.25.5. the atp-binding cassette (abc) superfamily.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAD35314 ; AAD35314 ; TM_0222 .
GeneIDi 18092577.
897116.
KEGGi tma:TM0222.
PATRICi 23935316. VBITheMar51294_0224.

Phylogenomic databases

eggNOGi COG1122.
InParanoidi Q9WY65.
KOi K16787.
OMAi EMVARET.
OrthoDBi EOG6T7N3V.

Miscellaneous databases

EvolutionaryTracei Q9WY65.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00005. ABC_tran. 1 hit.
[Graphical view ]
SMARTi SM00382. AAA. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
  2. "Purification, crystallization and preliminary X-ray diffraction analysis of the putative ABC transporter ATP-binding protein from Thermotoga maritima."
    Ethayathulla A.S., Bessho Y., Shinkai A., Padmanabhan B., Singh T.P., Kaur P., Yokoyama S.
    Acta Crystallogr. F 64:498-500(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
  3. "Assembly and mechanism of a group II ECF transporter."
    Karpowich N.K., Wang D.N.
    Proc. Natl. Acad. Sci. U.S.A. 110:2534-2539(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A TRANSPORT COMPONENT, SUBUNIT, SUBCELLULAR LOCATION, EXPRESSION IN E.COLI, ATP-BINDING, X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 2-266 IN COMPLEX WITH ADP.
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Entry informationi

Entry nameiECFA2_THEMA
AccessioniPrimary (citable) accession number: Q9WY65
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: November 1, 1999
Last modified: November 26, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Structure 4HLU is probably in the open state.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3