Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Energy-coupling factor transporter ATP-binding protein EcfA2

Gene

ecfA2

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

ATP-binding (A) component of a common energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates (Probable). Expression of the complex plus RibU in E.coli allows riboflavin uptake; uptake does not occur in the absence of RibU or the EcfA1A2T complex.Curated1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei164Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi43 – 48ATP6

GO - Molecular functioni

  • ATPase activity Source: InterPro
  • ATP binding Source: UniProtKB
  • riboflavin transporter activity Source: UniProtKB

GO - Biological processi

  • riboflavin transport Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Protein family/group databases

TCDBi3.A.1.25.5. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Energy-coupling factor transporter ATP-binding protein EcfA2 (EC:3.6.3.-)
Short name:
ECF transporter A component EcfA2
Gene namesi
Name:ecfA2
Synonyms:ecfA, ecfA'
Ordered Locus Names:TM_0222
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane 1 Publication; Peripheral membrane protein 1 Publication

GO - Cellular componenti

  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000921151 – 266Energy-coupling factor transporter ATP-binding protein EcfA2Add BLAST266

Interactioni

Subunit structurei

Forms a heterodimer with EcfA1. Forms a stable energy-coupling factor (ECF) transporter complex composed of 2 membrane-embedded substrate-binding proteins (S component, RibU, BioY), 2 ATP-binding proteins (A component) and 2 transmembrane proteins (T component) upon coexpression in E.coli. Stable subcomplexes with both A plus T components can also be isolated. This complex interacts with at least 2 substrate-specific components, BioY and RibU.1 Publication

Protein-protein interaction databases

DIPiDIP-61612N.
STRINGi243274.TM0222.

Structurei

Secondary structure

1266
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 13Combined sources11
Beta strandi20 – 30Combined sources11
Beta strandi35 – 39Combined sources5
Beta strandi42 – 44Combined sources3
Helixi46 – 53Combined sources8
Beta strandi60 – 66Combined sources7
Helixi73 – 76Combined sources4
Helixi77 – 79Combined sources3
Beta strandi80 – 83Combined sources4
Helixi87 – 90Combined sources4
Helixi96 – 102Combined sources7
Turni103 – 107Combined sources5
Helixi114 – 123Combined sources10
Helixi128 – 131Combined sources4
Helixi136 – 138Combined sources3
Helixi141 – 153Combined sources13
Beta strandi158 – 164Combined sources7
Turni165 – 168Combined sources4
Helixi171 – 186Combined sources16
Beta strandi190 – 194Combined sources5
Turni199 – 201Combined sources3
Helixi202 – 204Combined sources3
Beta strandi206 – 212Combined sources7
Beta strandi215 – 221Combined sources7
Helixi222 – 228Combined sources7
Helixi231 – 233Combined sources3
Helixi236 – 243Combined sources8
Turni244 – 247Combined sources4
Beta strandi252 – 254Combined sources3
Helixi257 – 260Combined sources4
Turni261 – 263Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YZ2X-ray2.30A/B1-266[»]
4HLUX-ray2.70A/B2-266[»]
4ZIRX-ray3.00A2-266[»]
ProteinModelPortaliQ9WY65.
SMRiQ9WY65.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WY65.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 238ABC transporterPROSITE-ProRule annotationAdd BLAST236

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni220 – 266Required for heterodimer formationAdd BLAST47

Sequence similaritiesi

Contains 1 ABC transporter domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108JJB. Bacteria.
COG1122. LUCA.
InParanoidiQ9WY65.
KOiK16787.
OMAiFETAYPH.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WY65-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIEVVNVSH IFHRGTPLEK KALENVSLVI NEGECLLVAG NTGSGKSTLL
60 70 80 90 100
QIVAGLIEPT SGDVLYDGER KKGYEIRRNI GIAFQYPEDQ FFAERVFDEV
110 120 130 140 150
AFAVKNFYPD RDPVPLVKKA MEFVGLDFDS FKDRVPFFLS GGEKRRVAIA
160 170 180 190 200
SVIVHEPDIL ILDEPLVGLD REGKTDLLRI VEKWKTLGKT VILISHDIET
210 220 230 240 250
VINHVDRVVV LEKGKKVFDG TRMEFLEKYD PRFFTSKMLV MRRLVLKGED
260
PFSMSDDELL ERVCNS
Length:266
Mass (Da):30,233
Last modified:November 1, 1999 - v1
Checksum:iEB146157FC21EB4F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35314.1.
PIRiA72401.
RefSeqiNP_228037.1. NC_000853.1.
WP_004082907.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD35314; AAD35314; TM_0222.
GeneIDi897116.
KEGGitma:TM0222.
PATRICi23935316. VBITheMar51294_0224.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35314.1.
PIRiA72401.
RefSeqiNP_228037.1. NC_000853.1.
WP_004082907.1. NZ_CP011107.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YZ2X-ray2.30A/B1-266[»]
4HLUX-ray2.70A/B2-266[»]
4ZIRX-ray3.00A2-266[»]
ProteinModelPortaliQ9WY65.
SMRiQ9WY65.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-61612N.
STRINGi243274.TM0222.

Protein family/group databases

TCDBi3.A.1.25.5. the atp-binding cassette (abc) superfamily.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD35314; AAD35314; TM_0222.
GeneIDi897116.
KEGGitma:TM0222.
PATRICi23935316. VBITheMar51294_0224.

Phylogenomic databases

eggNOGiENOG4108JJB. Bacteria.
COG1122. LUCA.
InParanoidiQ9WY65.
KOiK16787.
OMAiFETAYPH.

Miscellaneous databases

EvolutionaryTraceiQ9WY65.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiECFA2_THEMA
AccessioniPrimary (citable) accession number: Q9WY65
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: November 1, 1999
Last modified: November 2, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Structure 4HLU is probably in the open state.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.