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Q9WY65

- ECFA2_THEMA

UniProt

Q9WY65 - ECFA2_THEMA

Protein

Energy-coupling factor transporter ATP-binding protein EcfA2

Gene

ecfA2

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    ATP-binding (A) component of a common energy-coupling factor (ECF) ABC-transporter complex. Unlike classic ABC transporters this ECF transporter provides the energy necessary to transport a number of different substrates Probable. Expression of the complex plus RibU in E.coli allows riboflavin uptake; uptake does not occur in the absence of RibU or the EcfA1A2T complex.1 PublicationCurated

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei164 – 1641Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi43 – 486ATP

    GO - Molecular functioni

    1. ATPase activity Source: InterPro
    2. ATP binding Source: UniProtKB
    3. riboflavin transporter activity Source: UniProtKB

    GO - Biological processi

    1. riboflavin transport Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Transport

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Protein family/group databases

    TCDBi3.A.1.25.5. the atp-binding cassette (abc) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Energy-coupling factor transporter ATP-binding protein EcfA2 (EC:3.6.3.-)
    Short name:
    ECF transporter A component EcfA2
    Gene namesi
    Name:ecfA2
    Synonyms:ecfA, ecfA'
    Ordered Locus Names:TM_0222
    OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
    Taxonomic identifieri243274 [NCBI]
    Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
    ProteomesiUP000008183: Chromosome

    Subcellular locationi

    Cell inner membrane 1 Publication; Peripheral membrane protein 1 Publication

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 266266Energy-coupling factor transporter ATP-binding protein EcfA2PRO_0000092115Add
    BLAST

    Interactioni

    Subunit structurei

    Forms a heterodimer with EcfA1. Forms a stable energy-coupling factor (ECF) transporter complex composed of 2 membrane-embedded substrate-binding proteins (S component, RibU, BioY), 2 ATP-binding proteins (A component) and 2 transmembrane proteins (T component) upon coexpression in E.coli. Stable subcomplexes with both A plus T components can also be isolated. This complex interacts with at least 2 substrate-specific components, BioY and RibU.1 Publication

    Protein-protein interaction databases

    STRINGi243274.TM0222.

    Structurei

    Secondary structure

    1
    266
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 1311
    Beta strandi20 – 3011
    Beta strandi35 – 395
    Helixi46 – 538
    Beta strandi60 – 667
    Helixi73 – 764
    Helixi77 – 793
    Beta strandi80 – 834
    Helixi87 – 904
    Helixi96 – 1027
    Turni103 – 1075
    Helixi114 – 12310
    Helixi128 – 1314
    Helixi136 – 1383
    Helixi141 – 15313
    Beta strandi158 – 1647
    Turni165 – 1684
    Helixi171 – 18616
    Beta strandi190 – 1945
    Turni199 – 2013
    Helixi202 – 2043
    Beta strandi206 – 2127
    Beta strandi215 – 2217
    Helixi222 – 2287
    Helixi231 – 2333
    Helixi236 – 2438
    Turni244 – 2474
    Beta strandi252 – 2543
    Helixi257 – 2604
    Turni261 – 2633

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2YZ2X-ray2.30A/B1-266[»]
    4HLUX-ray2.70A/B2-266[»]
    ProteinModelPortaliQ9WY65.
    SMRiQ9WY65. Positions 1-266.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9WY65.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 238236ABC transporterPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni220 – 26647Required for heterodimer formationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 ABC transporter domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG1122.
    KOiK16787.
    OMAiEMVARET.
    OrthoDBiEOG6T7N3V.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR003593. AAA+_ATPase.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00005. ABC_tran. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
    PS50893. ABC_TRANSPORTER_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9WY65-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRIEVVNVSH IFHRGTPLEK KALENVSLVI NEGECLLVAG NTGSGKSTLL    50
    QIVAGLIEPT SGDVLYDGER KKGYEIRRNI GIAFQYPEDQ FFAERVFDEV 100
    AFAVKNFYPD RDPVPLVKKA MEFVGLDFDS FKDRVPFFLS GGEKRRVAIA 150
    SVIVHEPDIL ILDEPLVGLD REGKTDLLRI VEKWKTLGKT VILISHDIET 200
    VINHVDRVVV LEKGKKVFDG TRMEFLEKYD PRFFTSKMLV MRRLVLKGED 250
    PFSMSDDELL ERVCNS 266
    Length:266
    Mass (Da):30,233
    Last modified:November 1, 1999 - v1
    Checksum:iEB146157FC21EB4F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000512 Genomic DNA. Translation: AAD35314.1.
    PIRiA72401.
    RefSeqiNP_228037.1. NC_000853.1.
    YP_007976571.1. NC_021214.1.
    YP_008990804.1. NC_023151.1.

    Genome annotation databases

    EnsemblBacteriaiAAD35314; AAD35314; TM_0222.
    GeneIDi897116.
    KEGGitma:TM0222.
    tmi:THEMA_03615.
    tmm:Tmari_0220.
    PATRICi23935316. VBITheMar51294_0224.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000512 Genomic DNA. Translation: AAD35314.1 .
    PIRi A72401.
    RefSeqi NP_228037.1. NC_000853.1.
    YP_007976571.1. NC_021214.1.
    YP_008990804.1. NC_023151.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2YZ2 X-ray 2.30 A/B 1-266 [» ]
    4HLU X-ray 2.70 A/B 2-266 [» ]
    ProteinModelPortali Q9WY65.
    SMRi Q9WY65. Positions 1-266.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243274.TM0222.

    Protein family/group databases

    TCDBi 3.A.1.25.5. the atp-binding cassette (abc) superfamily.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAD35314 ; AAD35314 ; TM_0222 .
    GeneIDi 897116.
    KEGGi tma:TM0222.
    tmi:THEMA_03615.
    tmm:Tmari_0220.
    PATRICi 23935316. VBITheMar51294_0224.

    Phylogenomic databases

    eggNOGi COG1122.
    KOi K16787.
    OMAi EMVARET.
    OrthoDBi EOG6T7N3V.

    Miscellaneous databases

    EvolutionaryTracei Q9WY65.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR003593. AAA+_ATPase.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00005. ABC_tran. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00211. ABC_TRANSPORTER_1. 1 hit.
    PS50893. ABC_TRANSPORTER_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
    2. "Purification, crystallization and preliminary X-ray diffraction analysis of the putative ABC transporter ATP-binding protein from Thermotoga maritima."
      Ethayathulla A.S., Bessho Y., Shinkai A., Padmanabhan B., Singh T.P., Kaur P., Yokoyama S.
      Acta Crystallogr. F 64:498-500(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).
      Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
    3. "Assembly and mechanism of a group II ECF transporter."
      Karpowich N.K., Wang D.N.
      Proc. Natl. Acad. Sci. U.S.A. 110:2534-2539(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS A TRANSPORT COMPONENT, SUBUNIT, SUBCELLULAR LOCATION, EXPRESSION IN E.COLI, ATP-BINDING, X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 2-266 IN COMPLEX WITH ADP.
      Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

    Entry informationi

    Entry nameiECFA2_THEMA
    AccessioniPrimary (citable) accession number: Q9WY65
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 21, 2004
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Structure 4HLU is probably in the open state.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3