Q9WY60 (SYGB_THEMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine--tRNA ligase beta subunit
EC=6.1.1.14

Alternative name(s):
Glycyl-tRNA synthetase beta subunit
Short name=GlyRS

Gene names

Name:	glyS
Ordered Locus Names:	TM_0217

Organism

Thermotoga maritima

Taxonomic identifier

2336 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length	672 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). HAMAP MF_00255
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm HAMAP MF_00255.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glycyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW arginine-tRNA ligase activity Inferred from electronic annotation. Source: InterPro glycine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 672	672	Glycine--tRNA ligase beta subunit HAMAP MF_00255		PRO_0000072934

Sequences

	Sequence		Length	Mass (Da)
	Q9WY60 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 612B402052DD429A	FASTA	672	77,813
	10 20 30 40 50 60 MRTALLEVGL EELPASEFHS ILKQLEEKSA ELLKAYRVSS GSVEVFVGSR RFGVILKNLP 70 80 90 100 110 120 ERQEDFTEEK KGPPLNVAYD ENGKPTRALE GFLRNNNASL ENVVHREGYV YLSRVVEGKP 130 140 150 160 170 180 VEEVLPDLFR DLVLGLNFRK PMRWGSGEHE YIRPVHWIVA MVDGRVLDLE IFGLRSSRIS 190 200 210 220 230 240 YGKRYHAGSI EIPDSERYYE SLKKGFVISS HLERKKFVLE QIDEFEKRSS MKIERDEELI 250 260 270 280 290 300 EEIVAITEYP RIVVGQFDRK YLELPEEIIV TAVKHHQRSF IAHKGTLTNT FVAFQDGPQP 310 320 330 340 350 360 PENVVKGYER VINARLEDAR YYFQKDLETP LEKMNEKLKE IVFQEKLGTL YDKVERIKKI 370 380 390 400 410 420 SQRLCEDLKL PGSFTQKVLE AASICKADIA SKVVYEFPEL QGVMGRIYAL REGINEEIAT 430 440 450 460 470 480 AIEDHYSEEP QTVIGSILGI ADRIDTIVGN FAIGNVPTSS KDPYGLKSKA DTIFRIIRKN 490 500 510 520 530 540 EWDISLEELL TFASSLVGYR LSEELETFFA GRFYQFLINE LGISFDVARA VNHLWKKPLR 550 560 570 580 590 600 GILSAEALQE ISEKPEFQDL FVGFERVHNI TKNHDSTKFD GALFEKEEEK KLMNKFYEVK 610 620 630 640 650 660 EKVLKALERL NYREALQYLI ELKPYIDEYF DNVFVMVKRD DLRVNRLSFL KNIDELFMMV 670 GDMTYLVKRS QV

References

[1]

"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima." Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Nature 399:323-329(1999) [PubMed: 10360571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000512 Genomic DNA. Translation: AAD35309.1.
PIR	C72404.
RefSeq	NP_228032.1. NC_000853.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	897102.
GenomeReviews	Gene locus TM_0217 in contig AE000512_GR.
KEGG	tma:TM0217.
NMPDR	fig|243274.1.peg.216.
PATRIC	23935306. VBITheMar51294_0219.
TIGR	TM_0217.
Phylogenomic databases
HOGENOM	HBG586599.
OMA	FIRPVHT.
PhylomeDB	Q9WY60.
ProtClustDB	PRK01233.
Enzyme and pathway databases
BioCyc	TMAR243274:TM_0217-MONOMER.
Family and domain databases
HAMAP	MF_00255. Gly_tRNA_synth_beta. [Tree]
InterPro	IPR008909. DALR_anticod-bd. IPR006194. Gly-tRNA-synth_II_heterodimer. IPR015944. Gly-tRNA-synth_IIc_bsu. IPR023279. HD. [Graphical view]
Gene3D	G3DSA:1.10.3210.10. HD. 1 hit.
KO	K01879.
Pfam	PF05746. DALR_1. 1 hit. PF02092. tRNA_synt_2f. 1 hit. [Graphical view]
PRINTS	PR01045. TRNASYNTHGB.
TIGRFAMs	TIGR00211. GlyS. 1 hit.
PROSITE	PS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYGB_THEMA

Accession

Primary (citable) accession number: Q9WY60

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1999
Last modified:	January 25, 2012
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program