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Q9WY51

- KPYK_THEMA

UniProt

Q9WY51 - KPYK_THEMA

Protein

Pyruvate kinase

Gene

pyk

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Divalent metal cations.1 Publication

    Enzyme regulationi

    Allosterically activated by AMP and inhibited by ATP.

    Kineticsi

      Vmax=578 µmol/min/mg enzyme (at 65 degrees Celsius)1 Publication

      pH dependencei

      Optimum pH is 5.9-6.0.1 Publication

      Temperature dependencei

      Optimum temperature is 80 degrees Celsius for the recombinant enzyme. Thermostable up to 85 degrees Celsius.1 Publication

      Pathwayi

      Sites

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Binding sitei32 – 321SubstrateBy similarity
      Metal bindingi34 – 341PotassiumBy similarity
      Metal bindingi36 – 361PotassiumBy similarity
      Metal bindingi66 – 661PotassiumBy similarity
      Sitei217 – 2171Transition state stabilizerBy similarity
      Metal bindingi219 – 2191MagnesiumBy similarity
      Binding sitei242 – 2421Substrate; via amide nitrogenBy similarity
      Metal bindingi243 – 2431MagnesiumBy similarity
      Binding sitei243 – 2431Substrate; via amide nitrogenBy similarity
      Binding sitei275 – 2751SubstrateBy similarity

      GO - Molecular functioni

      1. ATP binding Source: UniProtKB-KW
      2. magnesium ion binding Source: InterPro
      3. potassium ion binding Source: InterPro
      4. pyruvate kinase activity Source: UniProtKB-EC

      GO - Biological processi

      1. glycolytic process Source: UniProtKB-UniPathway

      Keywords - Molecular functioni

      Kinase, Transferase

      Keywords - Biological processi

      Glycolysis

      Keywords - Ligandi

      ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

      Enzyme and pathway databases

      BioCyciMetaCyc:MONOMER-4887.
      TMAR243274:GC6P-221-MONOMER.
      UniPathwayiUPA00109; UER00188.

      Names & Taxonomyi

      Protein namesi
      Recommended name:
      Pyruvate kinase (EC:2.7.1.40)
      Short name:
      PK
      Gene namesi
      Name:pyk
      Ordered Locus Names:TM_0208
      OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
      Taxonomic identifieri243274 [NCBI]
      Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
      ProteomesiUP000008183: Chromosome

      PTM / Processingi

      Molecule processing

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Chaini1 – 466466Pyruvate kinasePRO_0000295179Add
      BLAST

      Interactioni

      Subunit structurei

      Homotetramer.1 Publication

      Protein-protein interaction databases

      STRINGi243274.TM0208.

      Structurei

      3D structure databases

      ProteinModelPortaliQ9WY51.
      ModBaseiSearch...
      MobiDBiSearch...

      Family & Domainsi

      Sequence similaritiesi

      Belongs to the pyruvate kinase family.Curated

      Phylogenomic databases

      eggNOGiCOG0469.
      KOiK00873.
      OMAiGTHEEHK.
      OrthoDBiEOG6GBMB0.

      Family and domain databases

      Gene3Di2.40.33.10. 1 hit.
      3.20.20.60. 2 hits.
      3.40.1380.20. 1 hit.
      InterProiIPR001697. Pyr_Knase.
      IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
      IPR011037. Pyrv_Knase-like_insert_dom.
      IPR015794. Pyrv_Knase_a/b.
      IPR015793. Pyrv_Knase_brl.
      IPR015795. Pyrv_Knase_C.
      IPR015806. Pyrv_Knase_insert_dom.
      [Graphical view]
      PANTHERiPTHR11817. PTHR11817. 1 hit.
      PfamiPF00224. PK. 1 hit.
      PF02887. PK_C. 1 hit.
      [Graphical view]
      PRINTSiPR01050. PYRUVTKNASE.
      SUPFAMiSSF50800. SSF50800. 1 hit.
      SSF51621. SSF51621. 2 hits.
      SSF52935. SSF52935. 1 hit.
      TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.

      Sequencei

      Sequence statusi: Complete.

      Q9WY51-1 [UniParc]FASTAAdd to Basket

      « Hide

      MRSTKIVCTV GPRTDSYEMI EKMIDLGVNV FRINTSHGDW NEQEQKILKI    50
      KDLREKKKKP VAILIDLAGP KIRTGYLEKE FVELKEGQIF TLTTKEILGN 100
      EHIVSVNLSS LPKDVKKGDT ILLSDGEIVL EVIETTDTEV KTVVKVGGKI 150
      THRRGVNVPT ADLSVESITD RDREFIKLGT LHDVEFFALS FVRKPEDVLK 200
      AKEEIRKHGK EIPVISKIET KKALERLEEI IKVSDGIMVA RGDLGVEIPI 250
      EEVPIVQKEI IKLSKYYSKP VIVATQILES MIENPFPTRA EVTDIANAIF 300
      DGADALLLTA ETAVGKHPLE AIKVLSKVAK EAEKKLEFFR TIEYDTSDIS 350
      EAISHACWQL SESLNAKLII TPTISGSTAV RVSKYNVSQP IVALTPEEKT 400
      YYRLSLVRKV IPVLAEKCSQ ELEFIEKGLK KVEEMGLAEK GDLVVLTSGV 450
      PGKVGTTNTI RVLKVD 466
      Length:466
      Mass (Da):51,892
      Last modified:November 1, 1999 - v1
      Checksum:i5083B7B28A5D8DEF
      GO

      Sequence databases

      Select the link destinations:
      EMBL
      GenBank
      DDBJ
      Links Updated
      AE000512 Genomic DNA. Translation: AAD35300.1.
      PIRiB72406.
      RefSeqiNP_228023.1. NC_000853.1.
      YP_007976557.1. NC_021214.1.
      YP_008990818.1. NC_023151.1.

      Genome annotation databases

      EnsemblBacteriaiAAD35300; AAD35300; TM_0208.
      GeneIDi897079.
      KEGGitma:TM0208.
      tmi:THEMA_03685.
      tmm:Tmari_0206.
      PATRICi23935288. VBITheMar51294_0210.

      Cross-referencesi

      Sequence databases

      Select the link destinations:
      EMBL
      GenBank
      DDBJ
      Links Updated
      AE000512 Genomic DNA. Translation: AAD35300.1 .
      PIRi B72406.
      RefSeqi NP_228023.1. NC_000853.1.
      YP_007976557.1. NC_021214.1.
      YP_008990818.1. NC_023151.1.

      3D structure databases

      ProteinModelPortali Q9WY51.
      ModBasei Search...
      MobiDBi Search...

      Protein-protein interaction databases

      STRINGi 243274.TM0208.

      Protocols and materials databases

      Structural Biology Knowledgebase Search...

      Genome annotation databases

      EnsemblBacteriai AAD35300 ; AAD35300 ; TM_0208 .
      GeneIDi 897079.
      KEGGi tma:TM0208.
      tmi:THEMA_03685.
      tmm:Tmari_0206.
      PATRICi 23935288. VBITheMar51294_0210.

      Phylogenomic databases

      eggNOGi COG0469.
      KOi K00873.
      OMAi GTHEEHK.
      OrthoDBi EOG6GBMB0.

      Enzyme and pathway databases

      UniPathwayi UPA00109 ; UER00188 .
      BioCyci MetaCyc:MONOMER-4887.
      TMAR243274:GC6P-221-MONOMER.

      Family and domain databases

      Gene3Di 2.40.33.10. 1 hit.
      3.20.20.60. 2 hits.
      3.40.1380.20. 1 hit.
      InterProi IPR001697. Pyr_Knase.
      IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
      IPR011037. Pyrv_Knase-like_insert_dom.
      IPR015794. Pyrv_Knase_a/b.
      IPR015793. Pyrv_Knase_brl.
      IPR015795. Pyrv_Knase_C.
      IPR015806. Pyrv_Knase_insert_dom.
      [Graphical view ]
      PANTHERi PTHR11817. PTHR11817. 1 hit.
      Pfami PF00224. PK. 1 hit.
      PF02887. PK_C. 1 hit.
      [Graphical view ]
      PRINTSi PR01050. PYRUVTKNASE.
      SUPFAMi SSF50800. SSF50800. 1 hit.
      SSF51621. SSF51621. 2 hits.
      SSF52935. SSF52935. 1 hit.
      TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
      ProtoNeti Search...

      Publicationsi

      1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
        Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
      2. "Comparative analysis of pyruvate kinases from the hyperthermophilic archaea Archaeoglobus fulgidus, Aeropyrum pernix, and Pyrobaculum aerophilum and the hyperthermophilic bacterium Thermotoga maritima: unusual regulatory properties in hyperthermophilic archaea."
        Johnsen U., Hansen T., Schoenheit P.
        J. Biol. Chem. 278:25417-25427(2003) [PubMed] [Europe PMC] [Abstract]
        Cited for: BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, SUBUNIT, REGULATION.
        Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

      Entry informationi

      Entry nameiKPYK_THEMA
      AccessioniPrimary (citable) accession number: Q9WY51
      Entry historyi
      Integrated into UniProtKB/Swiss-Prot: July 10, 2007
      Last sequence update: November 1, 1999
      Last modified: October 1, 2014
      This is version 78 of the entry and version 1 of the sequence. [Complete history]
      Entry statusiReviewed (UniProtKB/Swiss-Prot)
      Annotation programProkaryotic Protein Annotation Program

      Miscellaneousi

      Keywords - Technical termi

      Allosteric enzyme, Complete proteome, Reference proteome

      Documents

      1. PATHWAY comments
        Index of metabolic and biosynthesis pathways
      2. SIMILARITY comments
        Index of protein domains and families

      External Data

      Dasty 3