Q9WY51 (KPYK_THEMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyruvate kinase
Short name=PK
EC=2.7.1.40

Gene names

Name:	pyk
Ordered Locus Names:	TM_0208

Organism

Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) [Reference proteome] [HAMAP]

Taxonomic identifier

243274 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Thermotogae › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga › Thermotoga maritima

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Divalent metal cations. Ref.2
Enzyme regulation	Allosterically activated by AMP and inhibited by ATP.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Subunit structure	Homotetramer. Ref.2
Sequence similarities	Belongs to the pyruvate kinase family.
Biophysicochemical properties	Kinetic parameters: Vmax=578 µmol/min/mg enzyme (at 65 degrees Celsius) Ref.2 pH dependence: Optimum pH is 5.9-6.0. Temperature dependence: Optimum temperature is 80 degrees Celsius for the recombinant enzyme. Thermostable up to 85 degrees Celsius.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Potassium Pyruvate
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 466	466	Pyruvate kinase		PRO_0000295179
Sites
	Metal binding	34	1	Potassium By similarity
	Metal binding	36	1	Potassium By similarity
	Metal binding	66	1	Potassium By similarity
	Metal binding	219	1	Magnesium By similarity
	Metal binding	243	1	Magnesium By similarity
	Binding site	32	1	Substrate By similarity
	Binding site	242	1	Substrate; via amide nitrogen By similarity
	Binding site	243	1	Substrate; via amide nitrogen By similarity
	Binding site	275	1	Substrate By similarity
	Site	217	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9WY51 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 5083B7B28A5D8DEF	FASTA	466	51,892
	10 20 30 40 50 60 MRSTKIVCTV GPRTDSYEMI EKMIDLGVNV FRINTSHGDW NEQEQKILKI KDLREKKKKP 70 80 90 100 110 120 VAILIDLAGP KIRTGYLEKE FVELKEGQIF TLTTKEILGN EHIVSVNLSS LPKDVKKGDT 130 140 150 160 170 180 ILLSDGEIVL EVIETTDTEV KTVVKVGGKI THRRGVNVPT ADLSVESITD RDREFIKLGT 190 200 210 220 230 240 LHDVEFFALS FVRKPEDVLK AKEEIRKHGK EIPVISKIET KKALERLEEI IKVSDGIMVA 250 260 270 280 290 300 RGDLGVEIPI EEVPIVQKEI IKLSKYYSKP VIVATQILES MIENPFPTRA EVTDIANAIF 310 320 330 340 350 360 DGADALLLTA ETAVGKHPLE AIKVLSKVAK EAEKKLEFFR TIEYDTSDIS EAISHACWQL 370 380 390 400 410 420 SESLNAKLII TPTISGSTAV RVSKYNVSQP IVALTPEEKT YYRLSLVRKV IPVLAEKCSQ 430 440 450 460 ELEFIEKGLK KVEEMGLAEK GDLVVLTSGV PGKVGTTNTI RVLKVD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima." Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Nature 399:323-329(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[2]	"Comparative analysis of pyruvate kinases from the hyperthermophilic archaea Archaeoglobus fulgidus, Aeropyrum pernix, and Pyrobaculum aerophilum and the hyperthermophilic bacterium Thermotoga maritima: unusual regulatory properties in hyperthermophilic archaea." Johnsen U., Hansen T., Schoenheit P. J. Biol. Chem. 278:25417-25427(2003) [PubMed] [Europe PMC] [Abstract] Cited for: BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR, SUBUNIT, REGULATION. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000512 Genomic DNA. Translation: AAD35300.1.
PIR	B72406.
RefSeq	NP_228023.1. NC_000853.1.
3D structure databases
ProteinModelPortal	Q9WY51.
ModBase	Search...
Protein-protein interaction databases
STRING	243274.TM0208.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAD35300; AAD35300; TM_0208.
GeneID	897079.
KEGG	tma:TM0208.
PATRIC	23935288. VBITheMar51294_0210.
Phylogenomic databases
eggNOG	COG0469.
KO	K00873.
OMA	VFGIEQG.
ProtClustDB	CLSK794999.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-4887. TMAR243274:GC6P-221-MONOMER.
UniPathway	UPA00109; UER00188.
Family and domain databases
Gene3D	2.40.33.10. 1 hit. 3.20.20.60. 2 hits. 3.40.1380.20. 1 hit.
InterPro	IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase-like_dom. IPR011037. Pyrv_Knase-like_insert_dom. IPR015794. Pyrv_Knase_a/b. IPR015793. Pyrv_Knase_brl. IPR015795. Pyrv_Knase_C. IPR015806. Pyrv_Knase_insert_dom. [Graphical view]
PANTHER	PTHR11817. PTHR11817. 1 hit.
Pfam	PF00224. PK. 1 hit. PF02887. PK_C. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
SUPFAM	SSF50800. PK_B_barrel_like. 1 hit. SSF52935. Pyruvate_kinase. 1 hit. SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR01064. pyruv_kin. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPYK_THEMA

Accession

Primary (citable) accession number: Q9WY51

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	November 1, 1999
Last modified:	May 29, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program