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Protein

Pyruvate kinase

Gene

pyk

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

a divalent metal cation1 Publication

Enzyme regulationi

Allosterically activated by AMP and inhibited by ATP.

Kineticsi

    1. Vmax=578 µmol/min/mg enzyme (at 65 degrees Celsius)1 Publication

    pH dependencei

    Optimum pH is 5.9-6.0.1 Publication

    Temperature dependencei

    Optimum temperature is 80 degrees Celsius for the recombinant enzyme. Thermostable up to 85 degrees Celsius.1 Publication

    Pathwayi: glycolysis

    This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
    Proteins known to be involved in the 5 steps of the subpathway in this organism are:
    1. Glyceraldehyde-3-phosphate dehydrogenase (gap)
    2. Bifunctional PGK/TIM (pgk/tpi), Phosphoglycerate kinase (Tmari_0689)
    3. Probable 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (apgM), Probable 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (apgM)
    4. Enolase (eno), Enolase (eno)
    5. Pyruvate kinase (pyk), Pyruvate kinase (Tmari_0206)
    This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei32SubstrateBy similarity1
    Metal bindingi34PotassiumBy similarity1
    Metal bindingi36PotassiumBy similarity1
    Metal bindingi66PotassiumBy similarity1
    Sitei217Transition state stabilizerBy similarity1
    Metal bindingi219MagnesiumBy similarity1
    Binding sitei242Substrate; via amide nitrogenBy similarity1
    Metal bindingi243MagnesiumBy similarity1
    Binding sitei243Substrate; via amide nitrogenBy similarity1
    Binding sitei275SubstrateBy similarity1

    GO - Molecular functioni

    Complete GO annotation...

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-4887.
    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate kinase (EC:2.7.1.40)
    Short name:
    PK
    Gene namesi
    Name:pyk
    Ordered Locus Names:TM_0208
    OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
    Taxonomic identifieri243274 [NCBI]
    Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
    Proteomesi
    • UP000008183 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002951791 – 466Pyruvate kinaseAdd BLAST466

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    STRINGi243274.TM0208.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9WY51.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated

    Phylogenomic databases

    eggNOGiENOG4105CA9. Bacteria.
    COG0469. LUCA.
    InParanoidiQ9WY51.
    KOiK00873.
    OMAiGTHEEHK.

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9WY51-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRSTKIVCTV GPRTDSYEMI EKMIDLGVNV FRINTSHGDW NEQEQKILKI
    60 70 80 90 100
    KDLREKKKKP VAILIDLAGP KIRTGYLEKE FVELKEGQIF TLTTKEILGN
    110 120 130 140 150
    EHIVSVNLSS LPKDVKKGDT ILLSDGEIVL EVIETTDTEV KTVVKVGGKI
    160 170 180 190 200
    THRRGVNVPT ADLSVESITD RDREFIKLGT LHDVEFFALS FVRKPEDVLK
    210 220 230 240 250
    AKEEIRKHGK EIPVISKIET KKALERLEEI IKVSDGIMVA RGDLGVEIPI
    260 270 280 290 300
    EEVPIVQKEI IKLSKYYSKP VIVATQILES MIENPFPTRA EVTDIANAIF
    310 320 330 340 350
    DGADALLLTA ETAVGKHPLE AIKVLSKVAK EAEKKLEFFR TIEYDTSDIS
    360 370 380 390 400
    EAISHACWQL SESLNAKLII TPTISGSTAV RVSKYNVSQP IVALTPEEKT
    410 420 430 440 450
    YYRLSLVRKV IPVLAEKCSQ ELEFIEKGLK KVEEMGLAEK GDLVVLTSGV
    460
    PGKVGTTNTI RVLKVD
    Length:466
    Mass (Da):51,892
    Last modified:November 1, 1999 - v1
    Checksum:i5083B7B28A5D8DEF
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE000512 Genomic DNA. Translation: AAD35300.1.
    PIRiB72406.
    RefSeqiNP_228023.1. NC_000853.1.
    WP_008193903.1. NZ_CP011107.1.

    Genome annotation databases

    EnsemblBacteriaiAAD35300; AAD35300; TM_0208.
    GeneIDi897079.
    KEGGitma:TM0208.
    PATRICi23935288. VBITheMar51294_0210.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE000512 Genomic DNA. Translation: AAD35300.1.
    PIRiB72406.
    RefSeqiNP_228023.1. NC_000853.1.
    WP_008193903.1. NZ_CP011107.1.

    3D structure databases

    ProteinModelPortaliQ9WY51.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi243274.TM0208.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAD35300; AAD35300; TM_0208.
    GeneIDi897079.
    KEGGitma:TM0208.
    PATRICi23935288. VBITheMar51294_0210.

    Phylogenomic databases

    eggNOGiENOG4105CA9. Bacteria.
    COG0469. LUCA.
    InParanoidiQ9WY51.
    KOiK00873.
    OMAiGTHEEHK.

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00188.
    BioCyciMetaCyc:MONOMER-4887.

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiKPYK_THEMA
    AccessioniPrimary (citable) accession number: Q9WY51
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 10, 2007
    Last sequence update: November 1, 1999
    Last modified: November 2, 2016
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.