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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei583 – 5831ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:TM_0168
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
ProteomesiUP000008183: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 824824Leucine--tRNA ligasePRO_0000152106Add
BLAST

Proteomic databases

PRIDEiQ9WY15.

Interactioni

Protein-protein interaction databases

STRINGi243274.TM0168.

Structurei

3D structure databases

ProteinModelPortaliQ9WY15.
SMRiQ9WY15. Positions 1-761.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi41 – 5111"HIGH" regionAdd
BLAST
Motifi580 – 5845"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
InParanoidiQ9WY15.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WY15-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEYRPQEIE KKWQEVWEEK KVFYTPQRSE KPKYYALVMF PYPSGTLHVG
60 70 80 90 100
HVKNYVIGDI VARYKRMRGY NVLHPFGYDA FGLPAENAAI EKGIHPEEWT
110 120 130 140 150
RKNIATIRQQ VKKLGISYDW SREIATCDEE YYKWTQWIFL QLYKNGLAYK
160 170 180 190 200
KKAAVNWCPK CKTVLANEQV KDGKCERCGT SVTIRHLEQW FFKITDYAER
210 220 230 240 250
LLNDLDKLTG WPEHVKTMQR NWIGKSTGAE IDFPVEGSDT KIRVFTTRPD
260 270 280 290 300
TLWGVTFMAL APESPLVEEL VPEEKKEELQ EFLERVKQQD RFRRTSVEAE
310 320 330 340 350
KEGFFLGRYA INPVTGERIP IYVANYILME YGTGAIMGVP AHDQRDFSFA
360 370 380 390 400
KKYGIPIKVV IKPADKDLDP EKMEEAYEGE GIMVNSGPFD GTPSSEGIEK
410 420 430 440 450
VINWLEEKGI GKRSVQYKLR DWLISRQRYW GAPIPIIYCE KCGVVPVPEE
460 470 480 490 500
DLPVRLPKDV EFLPTGQSPL SFHEGFKKTK CPVCGGEAQR ETDTMDTFVD
510 520 530 540 550
SSWYFLRYVN PHLEDKPFEP DDVNYWLPVD QYIGGVEHAV LHLLYSRFVT
560 570 580 590 600
KVLHDLGYLN FDEPFTNLFT QGMIYKDGAK MSKSKGNVVS PDEMIEKYGA
610 620 630 640 650
DTLRMYILFM APPEKDAEWS DAGIEGVHRF VKRLWNTFYT VLPFVKEENT
660 670 680 690 700
ENLVLKNSTE KELRRKLHSI IKKITEDIEG GFKFNTAISG LMELVNHLSQ
710 720 730 740 750
YLNSVPQEEW NRKLLREIVE KLTLALSPFA PHLAEEFWHD LGNDSLVVQQ
760 770 780 790 800
SWPSYDPKAL EVEEVEIAIQ INGKVRDKVV VPVDISEEDL KRIVLERERV
810 820
KEYVDGKPIR KFIYVKGRIV NIVV
Length:824
Mass (Da):95,625
Last modified:November 1, 1999 - v1
Checksum:i7CB0252A76A844EC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35261.1.
PIRiF72408.
RefSeqiNP_227983.1. NC_000853.1.
YP_007976517.1. NC_021214.1.
YP_008990856.1. NC_023151.1.

Genome annotation databases

EnsemblBacteriaiAAD35261; AAD35261; TM_0168.
GeneIDi15493770.
18092642.
897007.
KEGGitma:TM0168.
tmi:THEMA_03960.
PATRICi23935184. VBITheMar51294_0169.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35261.1.
PIRiF72408.
RefSeqiNP_227983.1. NC_000853.1.
YP_007976517.1. NC_021214.1.
YP_008990856.1. NC_023151.1.

3D structure databases

ProteinModelPortaliQ9WY15.
SMRiQ9WY15. Positions 1-761.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243274.TM0168.

Proteomic databases

PRIDEiQ9WY15.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD35261; AAD35261; TM_0168.
GeneIDi15493770.
18092642.
897007.
KEGGitma:TM0168.
tmi:THEMA_03960.
PATRICi23935184. VBITheMar51294_0169.

Phylogenomic databases

eggNOGiCOG0495.
InParanoidiQ9WY15.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

Entry informationi

Entry nameiSYL_THEMA
AccessioniPrimary (citable) accession number: Q9WY15
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: March 4, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.