Q9WY06 (NTPA_THEMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

TM_0159

Organism

Thermotoga maritima

Taxonomic identifier

2336 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length	196 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 196

196

Non-canonical purine NTP pyrophosphatase HAMAP MF_01405

PRO_0000178252

Regions

Region

10 – 15

6

Substrate binding By similarity

Region

68 – 69

2

Substrate binding By similarity

Sites

Metal binding

68

1

Magnesium or manganese By similarity

Binding site

151

1

Substrate By similarity

Binding site

176

1

Substrate By similarity

Secondary structure

1

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196

Helix Strand Turn

Details...

Beta strand

4 – 9

6

Helix

13 – 20

8

Beta strand

27 – 31

5

Helix

45 – 60

16

Beta strand

64 – 73

10

Helix

74 – 76

3

Helix

81 – 83

3

Turn

84 – 90

7

Helix

93 – 103

11

Turn

104 – 106

3

Beta strand

110 – 121

12

Turn

122 – 125

4

Beta strand

126 – 138

13

Helix

151 – 153

3

Beta strand

154 – 156

3

Turn

163 – 165

3

Helix

167 – 169

3

Helix

170 – 173

4

Helix

175 – 190

16

Sequences

	Sequence		Length	Mass (Da)
	Q9WY06 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 6C6E4D11901F38A8	FASTA	196	22,338
	10 20 30 40 50 60 MKKLTVYLAT TNPHKVEEIK MIAPEWMEIL PSPEKIEVVE DGETFLENSV KKAVVYGKKL 70 80 90 100 110 120 KHPVMADDSG LVIYSLGGFP GVMSARFMEE HSYKEKMRTI LKMLEGKDRR AAFVCSATFF 130 140 150 160 170 180 DPVENTLISV EDRVEGRIAN EIRGTGGFGY DPFFIPDGYD KTFGEIPHLK EKISHRSKAF 190 RKLFSVLEKI LESENR

References

[1]

"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima." Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Nature 399:323-329(1999) [PubMed: 10360571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.

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Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE000512 Genomic DNA. Translation: AAD35252.1.

PIR

E72410.

RefSeq

NP_227974.1. NC_000853.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1VP2	X-ray	1.78	A/B	1-196	[»]

ProteinModelPortal

Q9WY06.

SMR

Q9WY06. Positions 3-191.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

896997.

GenomeReviews

Gene locus TM_0159 in contig AE000512_GR.

KEGG

tma:TM0159.

NMPDR

fig|243274.1.peg.158.

PATRIC

23935164. VBITheMar51294_0159.

TIGR

TM_0159.

Phylogenomic databases

HOGENOM

HBG697237.

OMA

GVYTADW.

PhylomeDB

Q9WY06.

ProtClustDB

CLSK875003.

Enzyme and pathway databases

BioCyc

TMAR243274:TM_0159-MONOMER.

Family and domain databases

HAMAP

MF_01405. Non_canon_purine_NTPase.
[Tree]

InterPro

IPR002637. Ham1p-like.
IPR020922. Nucleoside-triphosphatase.
[Graphical view]

KO

K02428.

PANTHER

PTHR11067. Ham1p_like. 1 hit.

Pfam

PF01725. Ham1p_like. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00042. TIGR00042. 1 hit.

ProtoNet

Search...

Entry information

Entry name

NTPA_THEMA

Accession

Primary (citable) accession number: Q9WY06

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	November 1, 1999
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program