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Protein

dITP/XTP pyrophosphatase

Gene

TM_0159

Organism
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.UniRule annotation

Catalytic activityi

XTP + H2O = XMP + diphosphate.UniRule annotation
dITP + H2O = dIMP + diphosphate.UniRule annotation
ITP + H2O = IMP + diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei68Proton acceptorUniRule annotation1
Metal bindingi68MagnesiumUniRule annotation1
Binding sitei69Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processNucleotide metabolism
LigandMagnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
dITP/XTP pyrophosphataseUniRule annotation (EC:3.6.1.66UniRule annotation)
Alternative name(s):
Non-canonical purine NTP pyrophosphataseUniRule annotation
Non-standard purine NTP pyrophosphataseUniRule annotation
Nucleoside-triphosphate diphosphataseUniRule annotation
Nucleoside-triphosphate pyrophosphataseUniRule annotation
Short name:
NTPaseUniRule annotation
Gene namesi
Ordered Locus Names:TM_0159
OrganismiThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Taxonomic identifieri243274 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeThermotoga
Proteomesi
  • UP000008183 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001782521 – 196dITP/XTP pyrophosphataseAdd BLAST196

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi243274.TM0159.

Structurei

Secondary structure

1196
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 9Combined sources6
Helixi13 – 20Combined sources8
Beta strandi27 – 31Combined sources5
Helixi45 – 60Combined sources16
Beta strandi64 – 73Combined sources10
Helixi74 – 76Combined sources3
Helixi81 – 83Combined sources3
Turni84 – 90Combined sources7
Helixi93 – 103Combined sources11
Turni104 – 106Combined sources3
Beta strandi110 – 121Combined sources12
Turni122 – 125Combined sources4
Beta strandi126 – 138Combined sources13
Helixi151 – 153Combined sources3
Beta strandi154 – 156Combined sources3
Turni163 – 165Combined sources3
Helixi167 – 169Combined sources3
Helixi170 – 173Combined sources4
Helixi175 – 190Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VP2X-ray1.78A/B1-196[»]
3S86X-ray2.15A/B/C/D1-196[»]
ProteinModelPortaliQ9WY06.
SMRiQ9WY06.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WY06.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni10 – 15Substrate bindingUniRule annotation6
Regioni148 – 151Substrate bindingUniRule annotation4
Regioni175 – 176Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the HAM1 NTPase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108V82. Bacteria.
COG0127. LUCA.
InParanoidiQ9WY06.
KOiK02428.
OMAiYDPIFQP.

Family and domain databases

CDDicd00515. HAM1. 1 hit.
Gene3Di3.90.950.10. 1 hit.
HAMAPiMF_01405. Non_canon_purine_NTPase. 1 hit.
InterProiView protein in InterPro
IPR020922. dITP/XTP_pyrophosphatase.
IPR002637. Ham1p-like.
IPR029001. ITPase-like_fam.
PANTHERiPTHR11067. PTHR11067. 1 hit.
PfamiView protein in Pfam
PF01725. Ham1p_like. 1 hit.
SUPFAMiSSF52972. SSF52972. 1 hit.
TIGRFAMsiTIGR00042. TIGR00042. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9WY06-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKLTVYLAT TNPHKVEEIK MIAPEWMEIL PSPEKIEVVE DGETFLENSV
60 70 80 90 100
KKAVVYGKKL KHPVMADDSG LVIYSLGGFP GVMSARFMEE HSYKEKMRTI
110 120 130 140 150
LKMLEGKDRR AAFVCSATFF DPVENTLISV EDRVEGRIAN EIRGTGGFGY
160 170 180 190
DPFFIPDGYD KTFGEIPHLK EKISHRSKAF RKLFSVLEKI LESENR
Length:196
Mass (Da):22,338
Last modified:November 1, 1999 - v1
Checksum:i6C6E4D11901F38A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000512 Genomic DNA. Translation: AAD35252.1.
PIRiE72410.
RefSeqiNP_227974.1. NC_000853.1.
WP_004082779.1. NZ_CP011107.1.

Genome annotation databases

EnsemblBacteriaiAAD35252; AAD35252; TM_0159.
GeneIDi29653140.
896997.
KEGGitma:TM0159.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiIXTPA_THEMA
AccessioniPrimary (citable) accession number: Q9WY06
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: November 1, 1999
Last modified: June 7, 2017
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families