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Protein

Phosphoenolpyruvate-protein phosphotransferase

Gene

ptsI

Organism
Streptococcus equinus (Streptococcus bovis)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr).By similarity

Miscellaneous

The reaction takes place in three steps, mediated by a phosphocarrier histidine residue located on the surface of the central domain. The two first partial reactions are catalyzed at an active site located on the N-terminal domain, and the third partial reaction is catalyzed at an active site located on the C-terminal domain. For catalytic turnover, the central domain swivels from the concave surface of the N-terminal domain to that of the C-terminal domain.By similarity

Catalytic activityi

Phosphoenolpyruvate + protein L-histidine = pyruvate + protein N(pi)-phospho-L-histidine.By similarity

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei191Tele-phosphohistidine intermediateBy similarity1
Binding sitei298PEPBy similarity1
Binding sitei334PEPBy similarity1
Metal bindingi435MagnesiumBy similarity1
Metal bindingi459MagnesiumBy similarity1
Binding sitei469PEPBy similarity1
Active sitei506Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processPhosphotransferase system, Sugar transport, Transport
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate-protein phosphotransferaseBy similarity (EC:2.7.3.9By similarity)
Alternative name(s):
Phosphotransferase system, enzyme IBy similarity
Gene namesi
Name:ptsI
OrganismiStreptococcus equinus (Streptococcus bovis)
Taxonomic identifieri1335 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001470901 – 577Phosphoenolpyruvate-protein phosphotransferaseAdd BLAST577

Proteomic databases

PRIDEiQ9WXK9

Expressioni

Inductioni

Transcription of ptsI appears to be regulated in response to sugars supplied, i.e. is four-fold lower when cells were grown on lactose than when grown on glucose.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9WXK9
SMRiQ9WXK9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni458 – 459PEP bindingBy similarity2

Domaini

The N-terminal domain contains the HPr binding site, the central domain the pyrophosphate/phosphate carrier histidine, and the C-terminal domain the pyruvate binding site.By similarity

Sequence similaritiesi

Belongs to the PEP-utilizing enzyme family.Curated

Phylogenomic databases

eggNOGiENOG4105BZ3 Bacteria
COG1080 LUCA

Family and domain databases

Gene3Di1.10.274.10, 1 hit
InterProiView protein in InterPro
IPR008279 PEP-util_enz_mobile_dom
IPR018274 PEP_util_AS
IPR000121 PEP_util_C
IPR023151 PEP_util_CS
IPR036637 Phosphohistidine_dom_sf
IPR024692 PTS_EI
IPR006318 PTS_EI-like
IPR008731 PTS_EIN
IPR036618 PtsI_HPr-bd_sf
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
PfamiView protein in Pfam
PF05524 PEP-utilisers_N, 1 hit
PF00391 PEP-utilizers, 1 hit
PF02896 PEP-utilizers_C, 1 hit
PIRSFiPIRSF000732 PTS_enzyme_I, 1 hit
SUPFAMiSSF47831 SSF47831, 1 hit
SSF51621 SSF51621, 1 hit
SSF52009 SSF52009, 1 hit
TIGRFAMsiTIGR01417 PTS_I_fam, 1 hit
PROSITEiView protein in PROSITE
PS00742 PEP_ENZYMES_2, 1 hit
PS00370 PEP_ENZYMES_PHOS_SITE, 1 hit

Sequencei

Sequence statusi: Complete.

Q9WXK9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEMLKGIAA SDGVAVAKAY LLVQPDLSFE TVTVEDTSAE EARLDAALKA
60 70 80 90 100
SQDELSIIRE KAVETLGEEA AAVFDAHLMV LADPEMISQI KETIRAKQTN
110 120 130 140 150
AEAGLKEVTD MFITIFEGME DNPYMQERAA DIRDVAKRVL AHLLGAKLPN
160 170 180 190 200
PATIDEESIV IAHDLTPSDT AQLNKQFVKA FVTNIGGRTS HSAIMARTLE
210 220 230 240 250
IAAVLGTNDI TSRVKDGDIV AVNGITGEVI INPTDEQVAE FKAAGEAYAK
260 270 280 290 300
QKAEWALLKD AKTVTADGKH FELAANIGTP KDVEGVNANG AEAVGLYRTE
310 320 330 340 350
FLYMDSQDFP TEDEQYEAYK AVLEGMNGKP VVVRTMDIGG DKELPYLDLP
360 370 380 390 400
KEMNPFLGFR ALRISISETG NAMFRTQIRA LLRASVHGQL RIMFPMVALL
410 420 430 440 450
KEFRAAKAIF DEEKANLKAE GVAVSDDIQV GIMIEIPAAA MLADQFAKEV
460 470 480 490 500
DFFSIGTNDL IQYTMAADRM NEQVSYLYQP YNPSILRLIN NVIKAAHAEG
510 520 530 540 550
KWVGMCGEMA GDQKAVPLLV EMGLDEFSMS ATSILRTRSL MKKLDTAKMQ
560 570
EYANRALTEC STMEEVLELS KEYVNVD
Length:577
Mass (Da):63,219
Last modified:November 1, 1999 - v1
Checksum:i989FF58D1B6DDD12
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB027569 Genomic DNA Translation: BAA78049.1

Similar proteinsi

Entry informationi

Entry nameiPT1_STREI
AccessioniPrimary (citable) accession number: Q9WXK9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: November 1, 1999
Last modified: May 23, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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