Reviewed, UniProtKB/Swiss-Prot Q9WXI7 (PTGA_BUCAI)

Names and origin

Protein names

Recommended name:
    Glucose-specific phosphotransferase enzyme IIA component
    EC=2.7.1.-
Alternative name(s):
    PTS system glucose-specific EIIA component
    EIIA-Glc
    EIII-Glc

Gene names

Name:	crr
Ordered Locus Names:	BU063

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	161 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucose transport By similarity.
Catalytic activity	Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine.
Subcellular location	Cytoplasm By similarity.
Domain	The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.
Sequence similarities	Contains 1 PTS EIIA type-1 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cytoplasm
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: InterPro
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 161	161	Glucose-specific phosphotransferase enzyme IIA component		PRO_0000186538
Regions
	Domain	31 – 135	105	PTS EIIA type-1
Sites
	Active site	83	1	Tele-phosphohistidine intermediate By similarity
	Site	68	1	Important for phospho-donor activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9WXI7-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: AB98270715EE08DC	FASTA	161	17,787
	10 20 30 40 50 60 MSFFSDFFNS KKTEIFAPLS GDIINIEDVP DPVFSKKIVG DGIAIKPSSN RILAPVNGTI 70 80 90 100 110 120 GKIFETMHAF SIISEDNVEL FIHFGIDTVK LKGEGFKKKA KDNQKVKIGD EIIILDLEFI 130 140 150 160 KEKAESILTP VVISNIENFK KIKKSSGTIA AGQTVIITLY H

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Buchnera sp. DNA for ptsH-ptsI-crr operon." Matsumoto K., Morioka M., Ishikawa H. Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB025229 Genomic DNA. Translation: BAA76880.1. BA000003 Genomic DNA. Translation: BAB12786.1.
RefSeq	NP_239900.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1109470.
GenomeReviews	Gene locus BU063 in contig BA000003_GR.
KEGG	buc:BU063.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9WXI7.
OMA	DEISCIV
Enzyme and pathway databases
BioCyc	BSP107806:BU063-MON.
Family and domain databases
InterPro	IPR011055. Dup_hybrid_motif. IPR001127. PTS_EIIA_1_perm. [Graphical view]
Pfam	PF00358. PTS_EIIA_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00830. PTBA. 1 hit.
PROSITE	PS51093. PTS_EIIA_TYPE_1. 1 hit. PS00371. PTS_EIIA_TYPE_1_HIS. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTGA_BUCAI

Accession

Primary (citable) accession number: Q9WXI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1999
Last modified:	November 24, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)