Reviewed, UniProtKB/Swiss-Prot Q9WXI5 (PTHP_BUCAI)

Names and origin

Protein names

Recommended name:
    Phosphocarrier protein HPr
    EC=2.7.11.-
Alternative name(s):
    Histidine-containing protein

Gene names

Name:	ptsH
Ordered Locus Names:	BU065

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	85 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The phosphoryl group from phosphoenolpyruvate (PEP) is transferred to the phosphoryl carrier protein HPr by enzyme I. Phospho-HPr then transfers it to the permease (enzymes II/III) By similarity.
Catalytic activity	Protein HPr N(pi)-phospho-L-histidine + protein EIIA = protein HPr + protein EIIA N(tau)-phospho-L-histidine.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the HPr family. Contains 1 HPr domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cytoplasm
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 85	85	Phosphocarrier protein HPr		PRO_0000107844
Regions
	Domain	1 – 85	85	HPr
Sites
	Active site	15	1	Pros-phosphohistidine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9WXI5-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: B496A6E6DAA976E4	FASTA	85	9,412
	10 20 30 40 50 60 MFQNQVKITA PHGLHTRPAA QFVKEAKKFT SEISIIYNGK SVNAKSLFKI QTLGLIQGSL 70 80 ITLSAEGEDE KKAIEHLSLI MTELE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Buchnera sp. DNA for ptsH-ptsI-crr operon." Matsumoto K., Morioka M., Ishikawa H. Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB025229 Genomic DNA. Translation: BAA76878.1. BA000003 Genomic DNA. Translation: BAB12788.1.
RefSeq	NP_239902.1.
3D structure databases
SMR	Q9WXI5. Positions 1-85.
ModBase	Search...
Genome annotation databases
GeneID	1109472.
GenomeReviews	Gene locus BU065 in contig BA000003_GR.
KEGG	buc:BU065.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9WXI5.
OMA	PLGIHAR
Enzyme and pathway databases
BioCyc	BSP107806:BU065-MON.
Family and domain databases
InterPro	IPR001020. PTS_HPr_His_P_site. IPR005698. PTS_HPr_prot. IPR000032. PTS_HPr_prot-like. IPR002114. PTS_HPr_Ser_P_site. [Graphical view]
Gene3D	G3DSA:3.30.1340.10. PTS_HPr_protein. 1 hit.
Pfam	PF00381. PTS-HPr. 1 hit. [Graphical view]
PRINTS	PR00107. PHOSPHOCPHPR.
TIGRFAMs	TIGR01003. PTS_HPr_family. 1 hit.
PROSITE	PS51350. PTS_HPR_DOM. 1 hit. PS00369. PTS_HPR_HIS. 1 hit. PS00589. PTS_HPR_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTHP_BUCAI

Accession

Primary (citable) accession number: Q9WXI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1999
Last modified:	November 24, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)