Reviewed, UniProtKB/Swiss-Prot Q9WXE6 (LINE_PSEPA)

Names and origin

Protein names

Recommended name:
    Chlorohydroquinone/hydroquinone 1,2-dioxygenase
    EC=1.13.11.-
Alternative name(s):
    Hydroquinone meta-cleavage dioxygenase

Gene names

Name:

linE

Organism

Pseudomonas paucimobilis (Sphingomonas paucimobilis)

Taxonomic identifier

13689 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Sphingomonas

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cleaves aromatic rings with two hydroxyl groups at para positions. Is involved in the degradation of both chlorohydroquinone (CHQ) and hydroquinone (HQ), producing maleylacetate and gamma-hydroxymuconic semialdehyde, respectively, with consumption of O2. Is not able to convert catechol, contrary to other meta-cleavage dioxygenases.
Cofactor	Binds 1 Fe2+ ion By similarity.
Pathway	Xenobiotic degradation; gamma-hexachlorocyclohexane degradation.
Induction	By CHQ, HQ, and 2,5-DCHQ.
Sequence similarities	Belongs to the extradiol ring-cleavage dioxygenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism Detoxification
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to toxin Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 321	321	Chlorohydroquinone/hydroquinone 1,2-dioxygenase		PRO_0000085042
Sites
	Metal binding	162	1	Iron Probable
	Metal binding	229	1	Iron Probable
	Metal binding	278	1	Iron Probable
Experimental info
	Mutagenesis	162	1	H → A: Loss of activity. Ref.1
	Mutagenesis	229	1	H → A: Loss of activity. Ref.1
	Mutagenesis	278	1	E → A: Loss of activity. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9WXE6-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 26ED662CB8B62826	FASTA	321	35,958
	10 20 30 40 50 60 MMQLPERVEG LHHITVATGS AQGDVDLLVK TLGQRLVKKT MFYDGARPVY HLYFGNELGE 70 80 90 100 110 120 PGTLYTTFPV RQAGYTGKRG AGQISAVSYN APVGTLSWWQ EHLIKRAVTV SEVRERFGQK 130 140 150 160 170 180 YLSFEHPDCG VGFEIIEQDT DGQFEPWDSP YVPKEVALRG FHSWTATLNR NEEMDSFMRN 190 200 210 220 230 240 AWNLKPQGRD GNYQRYAFGN GGAAKVLDVY IDEDERPGTW ALGEGQVHHA AFEVADLDVQ 250 260 270 280 290 300 AALKFDVEGL GYTDFSDRKH RGYFESIYVR TPGGVLFEAS VTLGFTHDES PEKLGSEVKV 310 320 APQLEGVKDE LLRTMNDPIV I

References

[1]

"Cloning and sequencing of a novel meta-cleavage dioxygenase gene whose product is involved in degradation of gamma-hexachlorocyclohexane in Sphingomonas paucimobilis." Miyauchi K., Adachi Y., Nagata Y., Takagi M. J. Bacteriol. 181:6712-6719(1999) [PubMed: 10542173] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF HIS-162; HIS-229 AND GLU-278. Strain: UT26.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB021867 Genomic DNA. Translation: BAA76672.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR000486. Xdiol_dOase_1_2. [Graphical view]
ProDom	PD002334. Gly_diox. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00082. EXTRADIOL_DIOXYGENAS. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LINE_PSEPA

Accession

Primary (citable) accession number: Q9WXE6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 3, 2003
Last sequence update:	November 1, 1999
Last modified:	November 4, 2008
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)