ID   RRF_THET8               Reviewed;         185 AA.
AC   Q9WX76; Q5SJZ4;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   27-MAR-2024, entry version 137.
DE   RecName: Full=Ribosome-recycling factor {ECO:0000255|HAMAP-Rule:MF_00040};
DE            Short=RRF {ECO:0000255|HAMAP-Rule:MF_00040};
DE   AltName: Full=Ribosome-releasing factor {ECO:0000255|HAMAP-Rule:MF_00040};
GN   Name=frr {ECO:0000255|HAMAP-Rule:MF_00040};
GN   OrderedLocusNames=TTHA0858;
OS   Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8).
OC   Bacteria; Deinococcota; Deinococci; Thermales; Thermaceae; Thermus.
OX   NCBI_TaxID=300852;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=10214965; DOI=10.1016/s0014-5793(99)00302-6;
RA   Fujiwara T., Ito K., Nakayashiki T., Nakamura Y.;
RT   "Amber mutations in ribosome recycling factors of Escherichia coli and
RT   Thermus thermophilus: evidence for C-terminal modulator element.";
RL   FEBS Lett. 447:297-302(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27634 / DSM 579 / HB8;
RA   Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T.,
RA   Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.;
RT   "Complete genome sequence of Thermus thermophilus HB8.";
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).
RX   PubMed=11073219; DOI=10.1017/s1355838200001060;
RA   Toyoda T., Tin O.F., Ito K., Fujiwara T., Kumasaka T., Yamamoto M.,
RA   Garber M.B., Nakamura Y.;
RT   "Crystal structure combined with genetic analysis of the Thermus
RT   thermophilus ribosome recycling factor shows that a flexible hinge may act
RT   as a functional switch.";
RL   RNA 6:1432-1444(2000).
CC   -!- FUNCTION: Responsible for the release of ribosomes from messenger RNA
CC       at the termination of protein biosynthesis. May increase the efficiency
CC       of translation by recycling ribosomes from one round of translation to
CC       another. {ECO:0000255|HAMAP-Rule:MF_00040}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00040}.
CC   -!- SIMILARITY: Belongs to the RRF family. {ECO:0000255|HAMAP-
CC       Rule:MF_00040}.
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DR   EMBL; AB016498; BAA76865.1; -; Genomic_DNA.
DR   EMBL; AP008226; BAD70681.1; -; Genomic_DNA.
DR   RefSeq; WP_011172951.1; NC_006461.1.
DR   RefSeq; YP_144124.1; NC_006461.1.
DR   PDB; 1EH1; X-ray; 2.60 A; A=1-185.
DR   PDB; 3J0D; EM; 11.10 A; J=1-185.
DR   PDB; 3J0E; EM; 9.90 A; G=1-185.
DR   PDB; 4V54; X-ray; 3.30 A; B6/D6=1-185.
DR   PDB; 4V55; X-ray; 4.00 A; B6/D6=1-185.
DR   PDB; 4V5A; X-ray; 3.50 A; AY/CY=1-185.
DR   PDB; 4V5Y; X-ray; 4.45 A; B6/D6=1-185.
DR   PDB; 6UCQ; X-ray; 3.50 A; 1w/2w=1-185.
DR   PDBsum; 1EH1; -.
DR   PDBsum; 3J0D; -.
DR   PDBsum; 3J0E; -.
DR   PDBsum; 4V54; -.
DR   PDBsum; 4V55; -.
DR   PDBsum; 4V5A; -.
DR   PDBsum; 4V5Y; -.
DR   PDBsum; 6UCQ; -.
DR   AlphaFoldDB; Q9WX76; -.
DR   BMRB; Q9WX76; -.
DR   EMDB; EMD-1915; -.
DR   EMDB; EMD-1916; -.
DR   SMR; Q9WX76; -.
DR   IntAct; Q9WX76; 3.
DR   EnsemblBacteria; BAD70681; BAD70681; BAD70681.
DR   GeneID; 3170111; -.
DR   KEGG; ttj:TTHA0858; -.
DR   PATRIC; fig|300852.9.peg.852; -.
DR   eggNOG; COG0233; Bacteria.
DR   HOGENOM; CLU_073981_2_0_0; -.
DR   PhylomeDB; Q9WX76; -.
DR   EvolutionaryTrace; Q9WX76; -.
DR   Proteomes; UP000000532; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0006415; P:translational termination; IEA:UniProtKB-UniRule.
DR   CDD; cd00520; RRF; 1.
DR   Gene3D; 3.30.1360.40; -; 1.
DR   Gene3D; 1.10.132.20; Ribosome-recycling factor; 1.
DR   HAMAP; MF_00040; RRF; 1.
DR   InterPro; IPR002661; Ribosome_recyc_fac.
DR   InterPro; IPR023584; Ribosome_recyc_fac_dom.
DR   InterPro; IPR036191; RRF_sf.
DR   NCBIfam; TIGR00496; frr; 1.
DR   PANTHER; PTHR20982:SF3; MITOCHONDRIAL RIBOSOME RECYCLING FACTOR; 1.
DR   PANTHER; PTHR20982; RIBOSOME RECYCLING FACTOR; 1.
DR   Pfam; PF01765; RRF; 1.
DR   SUPFAM; SSF55194; Ribosome recycling factor, RRF; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..185
FT                   /note="Ribosome-recycling factor"
FT                   /id="PRO_0000167567"
FT   HELIX           3..26
FT                   /evidence="ECO:0007829|PDB:1EH1"
FT   HELIX           36..38
FT                   /evidence="ECO:0007829|PDB:1EH1"
FT   STRAND          42..45
FT                   /evidence="ECO:0007829|PDB:1EH1"
FT   STRAND          48..51
FT                   /evidence="ECO:0007829|PDB:1EH1"
FT   HELIX           52..54
FT                   /evidence="ECO:0007829|PDB:1EH1"
FT   STRAND          57..59
FT                   /evidence="ECO:0007829|PDB:1EH1"
FT   STRAND          65..69
FT                   /evidence="ECO:0007829|PDB:1EH1"
FT   HELIX           73..83
FT                   /evidence="ECO:0007829|PDB:1EH1"
FT   STRAND          84..86
FT                   /evidence="ECO:0007829|PDB:1EH1"
FT   STRAND          92..95
FT                   /evidence="ECO:0007829|PDB:1EH1"
FT   STRAND          98..102
FT                   /evidence="ECO:0007829|PDB:1EH1"
FT   HELIX           110..147
FT                   /evidence="ECO:0007829|PDB:1EH1"
FT   HELIX           151..183
FT                   /evidence="ECO:0007829|PDB:1EH1"
SQ   SEQUENCE   185 AA;  20994 MW;  F3135ABF7F813328 CRC64;
     MTLKELYAET RSHMQKSLEV LEHNLAGLRT GRANPALLLH LKVEYYGAHV PLNQIATVTA
     PDPRTLVVQS WDQNALKAIE KAIRDSDLGL NPSNKGDALY INIPPLTEER RKDLVRAVRQ
     YAEEGRVAIR NIRREALDKL KKLAKELHLS EDETKRAEAE IQKITDEFIA KADQLAEKKE
     QEILG
//