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Q9WX75 (BCSA5_GLUXY) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative cellulose synthase 3

Including the following 2 domains:

  1. Cellulose synthase catalytic subunit [UDP-forming]
    EC=2.4.1.12
  2. Cyclic di-GMP-binding domain
    Alternative name(s):
    Cellulose synthase 3 regulatory subunit
Gene names
Name:bcsABII-B
OrganismGluconacetobacter xylinus (Acetobacter xylinus)
Taxonomic identifier28448 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeGluconacetobacter

Protein attributes

Sequence length1518 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

UDP-glucose + (1,4-beta-D-glucosyl)(n) = UDP + (1,4-beta-D-glucosyl)(n+1).

Pathway

Glycan metabolism; bacterial cellulose biosynthesis.

Subcellular location

Cell inner membrane; Multi-pass membrane protein Potential.

Domain

There are two conserved domains in the globular part of the catalytic subunit: the N-terminal domain (domain A) contains the conserved DXD motif and is possibly involved in catalysis and substrate binding. The C-terminal domain (domain B) contains the QXXRW motif and is present only in processive glycosyl transferases. It could be involved in the processivity function of the enzyme, possibly required for holding the growing glycan chain in the active site.

Sequence similarities

In the N-terminal section; belongs to the glycosyltransferase 2 family.

In the C-terminal section; belongs to the AcsB/BcsB family.

Contains 1 PilZ domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 15181518Putative cellulose synthase 3
PRO_0000059266

Regions

Transmembrane24 – 4421Helical; Potential
Transmembrane71 – 9121Helical; Potential
Transmembrane105 – 12521Helical; Potential
Transmembrane404 – 42421Helical; Potential
Transmembrane428 – 44821Helical; Potential
Transmembrane465 – 48521Helical; Potential
Transmembrane514 – 53421Helical; Potential
Transmembrane543 – 56321Helical; Potential
Transmembrane1481 – 150121Helical; Potential
Domain569 – 668100PilZ
Region1 – 731731Catalytic
Region144 – 23794Catalytic subdomain A
Region314 – 37461Catalytic subdomain B
Region732 – 1518787Cyclic di-GMP binding By similarity

Sites

Active site1861 Potential
Active site3301 Potential
Site2331Important for substrate binding Potential
Site2351Important for substrate binding Potential

Sequences

Sequence LengthMass (Da)Tools
Q9WX75 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 7D7634503183DAB6

FASTA1,518166,465
        10         20         30         40         50         60 
MYGTWFTTGK VTDLLARTGL DRVPVWVPVV LGVVLMAFVG SVRIDPALQG WVSIGTVTLL 

        70         80         90        100        110        120 
LVLNRRRGRG ITVFLMMLSL LVSLRYIVWR LTATVQFSNW LQTALAVLLL LAEAYALMTL 

       130        140        150        160        170        180 
CLSYFQMAWP LRRREHPLPE DMAQWPSVDV FVPSYNEELS LVRSTVLGAL DLDWPADRLN 

       190        200        210        220        230        240 
VYILDDGRRK AFHDFAVEAG AGYIIRAENN HAKAGNLNHA LAVTDSPFAV IFDCDHVPTR 

       250        260        270        280        290        300 
GFLRRTIGWM MADPNLALLQ TPHHFYAPDP FQRNLAGGMH VPPEGNMFYG LVQDGNDFWD 

       310        320        330        340        350        360 
ATFFCGSCAI IRREAVMGIG GFATETVTED AHTALKMQRR GWGTAYLREP LAAGLATERL 

       370        380        390        400        410        420 
ILHIGQRVRW ARGMIQIMRL DNPMLGAGLR WEQRLCYLSA MSHFLFAIPR LTFLVSPLAF 

       430        440        450        460        470        480 
LFLGQNIIAA SPLAISVYAL PHIFHSVITL SRIEGRWRYS FWSEIYETSL ALFLVRITIV 

       490        500        510        520        530        540 
TLLQPHKGKF NVTDKGGLLA RGYFDWDAVY PNVILAGVLC AALLRGVFGI VWQFHDRLAL 

       550        560        570        580        590        600 
QSFILNTLWV VISLIIVLAS IAVGRETRQT RNAPRVSVRL PVVVTDAHGR QMEGHTHDIS 

       610        620        630        640        650        660 
LGGLAVGTRL ATPDMVGGEV TVRYDSARDG IHVGVPARVL DARDGTLRLR WAVRDLEDER 

       670        680        690        700        710        720 
QVVSMVFGRN DAWAGWADFA PDRPLRSLAM VFRSIGGLLR RRPAEAPRAL HEMGEGELPA 

       730        740        750        760        770        780 
TEEKLEKQSF VLKPVPRSAR HGATASAALF VAFTALVPAA MAQEAPSPDQ SGVTAETPFG 

       790        800        810        820        830        840 
DSNTGVVPDA LPAIDPAVAD RISDAEVTRT LTFRNLGATT GPLTLRGYSP LQGLDVVVPA 

       850        860        870        880        890        900 
NRVVTHAQLT LSGALSPSLL PEANAVTVTL NEQYVGTLKV DPQHPQFGPV SFDIDPLYFT 

       910        920        930        940        950        960 
GDNKLNFHFA GEYRRDCNDL FNEILWARIS DMSRITLTTV RITPERKLSR LPAPFFDPNQ 

       970        980        990       1000       1010       1020 
RSTLRVPVVL PATGDRGALR AAGLVASWFG RIADFRKLSF PVSTTIPASG NAVEVGVNLP 

      1030       1040       1050       1060       1070       1080 
VDAEGGRPAG PMLAEVANPN DRWGTVLVVT GRTAQEVEVA ARALVFSPDT LGGVASKVVS 

      1090       1100       1110       1120       1130       1140 
DVSLETRHPY DAPAFVPTDR PVRFGELVGA ADLQGGGFAP AGMTLPFHLP PDLYTWRGRP 

      1150       1160       1170       1180       1190       1200 
FLMNMWVRAP GGPVVDLETS RVDVSLNNNY LQSYTLSPPG LWRKWSERLV NQHAGAVGHV 

      1210       1220       1230       1240       1250       1260 
TALPPWLLFG QNQLQFNFDA RPIDRGACRR TPGDIHMSVD SDSTLDFRRG YHFAEMPNLS 

      1270       1280       1290       1300       1310       1320 
YFAEAAFPFS RMADLSETTV VLPDHPDTGT TGAFLDLMGF FGASTWYPAA GVTVMGADEV 

      1330       1340       1350       1360       1370       1380 
AQTPPKGDIV VLGTAAQLGG AASGLLARSP YVIHDRHITV GQRMGLQGIW YLFQDHDHAG 

      1390       1400       1410       1420       1430       1440 
LKDGVTANLN APIAEAGVLL AAQSPYDSQR SVVAFTGDTP ERIHDLVLSL RNKGDLPSLQ 

      1450       1460       1470       1480       1490       1500 
GDLVLKNGDR FTSYRTAPVY TVGSLPLWLR LDWFLGHHPS ALYLAGLAGA GLAALGVWAW 

      1510 
LRGWSRRRIA RDDLTGEL 

« Hide

References

[1]"Cloning of cellulose synthase genes from Acetobacter xylinum JCM 7664: implication of a novel set of cellulose synthase genes."
Umeda Y., Hirano A., Ishibashi M., Akiyama H., Onizuka T., Ikeuchi M., Inoue Y.
DNA Res. 6:109-115(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: JCM 7664 / NBRC 13693.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB015804 Genomic DNA. Translation: BAA77600.1.

3D structure databases

ProteinModelPortalQ9WX75.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGT2. Glycosyltransferase Family 2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00694.

Family and domain databases

InterProIPR003919. Cell_synth_A.
IPR003920. Cell_synth_B.
IPR018513. Cell_synthase_bac.
IPR009875. PilZ_domain.
[Graphical view]
PfamPF03170. BcsB. 1 hit.
PF07238. PilZ. 1 hit.
[Graphical view]
PRINTSPR01440. CELLSNTHASEB.
TIGRFAMsTIGR03030. CelA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBCSA5_GLUXY
AccessionPrimary (citable) accession number: Q9WX75
Entry history
Integrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: November 1, 1999
Last modified: February 19, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways