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Protein

Cellulose synthase 1 catalytic subunit [UDP-forming]

Gene

bcsAI

Organism
Komagataeibacter xylinus (Gluconacetobacter xylinus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic subunit of cellulose synthase. It polymerizes uridine 5'-diphosphate glucose to cellulose. The thick cellulosic mats generated by this enzyme probably provide a specialized protective environment to the bacterium (By similarity).By similarity

Catalytic activityi

UDP-glucose + (1,4-beta-D-glucosyl)(n) = UDP + (1,4-beta-D-glucosyl)(n+1).

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by bis-(3'-5') cyclic diguanylic acid (c-di-GMP).By similarity

Pathwayi: bacterial cellulose biosynthesis

This protein is involved in the pathway bacterial cellulose biosynthesis, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway bacterial cellulose biosynthesis and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei189Sequence analysis1
Binding sitei236SubstrateSequence analysis1
Binding sitei238SubstrateSequence analysis1
Active sitei333Sequence analysis1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Cellulose biosynthesis

Keywords - Ligandi

c-di-GMP

Enzyme and pathway databases

UniPathwayiUPA00694.

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Cellulose synthase 1 catalytic subunit [UDP-forming] (EC:2.4.1.12)
Gene namesi
Name:bcsAI
OrganismiKomagataeibacter xylinus (Gluconacetobacter xylinus)
Taxonomic identifieri28448 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodospirillalesAcetobacteraceaeKomagataeibacter

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei29 – 49HelicalSequence analysisAdd BLAST21
Transmembranei106 – 126HelicalSequence analysisAdd BLAST21
Transmembranei153 – 173HelicalSequence analysisAdd BLAST21
Transmembranei407 – 427HelicalSequence analysisAdd BLAST21
Transmembranei430 – 450HelicalSequence analysisAdd BLAST21
Transmembranei468 – 488HelicalSequence analysisAdd BLAST21
Transmembranei515 – 535HelicalSequence analysisAdd BLAST21
Transmembranei547 – 567HelicalSequence analysisAdd BLAST21
Transmembranei649 – 669HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000592641 – 745Cellulose synthase 1 catalytic subunit [UDP-forming]Add BLAST745

Structurei

3D structure databases

ProteinModelPortaliQ9WX61.
SMRiQ9WX61.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini572 – 670PilZAdd BLAST99

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni147 – 240Catalytic subdomain AAdd BLAST94
Regioni317 – 377Catalytic subdomain BAdd BLAST61

Domaini

There are two conserved domains in the globular part of the catalytic subunit: the N-terminal domain (domain A) contains the conserved DXD motif and is possibly involved in catalysis and substrate binding. The C-terminal domain (domain B) contains the QXXRW motif and is present only in processive glycosyl transferases. It could be involved in the processivity function of the enzyme, possibly required for holding the growing glycan chain in the active site.

Sequence similaritiesi

Belongs to the glycosyltransferase 2 family.Curated
Contains 1 PilZ domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003919. Cell_synth_A.
IPR005150. Cellulose_synth.
IPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR009875. PilZ_domain.
[Graphical view]
PfamiPF03552. Cellulose_synt. 1 hit.
PF00535. Glycos_transf_2. 1 hit.
PF07238. PilZ. 1 hit.
[Graphical view]
PRINTSiPR01439. CELLSNTHASEA.
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR03030. CelA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9WX61-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEVQSSAPA ESWFGRFSNK ILSLRGASYV VGALGLCALL AATMVTLSLN
60 70 80 90 100
EQMIVALVCV AVFFIVGRRK SRRTQVFLEV LSALVSLRYL TWRLTETLDF
110 120 130 140 150
DTWTQGILGV TLLLAELYAL YMLFLSYFQT ISPLHRAPLP LPANPDEWPT
160 170 180 190 200
VDIFIPTYDE ALSIVRLTVL GALGIDWPPD KVNVYILDDG RREEFARFAE
210 220 230 240 250
ACGARYIARP DNAHAKAGNL NYAIKHTTGD HILILDCDHI PTRAFLQISM
260 270 280 290 300
GWMVSDSNIA LLQTPHHFYS PDPFQRNLAV GYRTPPEGNL FYGVIQDGND
310 320 330 340 350
FWDATFFCGS CAILRRKAIE EIGGFATETV TEDAHTALRM QRKGWSTAYL
360 370 380 390 400
RIPLASGLAT ERLITHIGQR MRWARGMIQI FRVDNPMLGS GLKLGQRLCY
410 420 430 440 450
LSAMTSFFFA IPRVIFLASP LAFLFFSQNI IAASPLAVGV YAIPHMFHSI
460 470 480 490 500
ATAAKVNKGW RYSFWSEVYE TVMALFLVRV TIVTMLFPSK GKFNVTEKGG
510 520 530 540 550
VLEREEFDLT ATYPNIIFAI IMALGLLRGL YALIFQHLDI ISERAYALNC
560 570 580 590 600
IWSVISLIIL MAVISVGRET KQLRQSHRIE AQIPVTVYDY DGNSSHGITE
610 620 630 640 650
DVSMGGVAIH LPWREVTPDH PVQVVIHAVL DGEEMNLPAT MIRSAQGKAV
660 670 680 690 700
FTWSISNIQV EAAVVRFVFG RADAWLQWNN YEDDRPLRSL WSLILSIKAL
710 720 730 740
FRRKGQMIAH SRPKKKPIAL PVERREPTTS QGGQKQEGKI SRAAS
Length:745
Mass (Da):83,519
Last modified:November 1, 1999 - v1
Checksum:i57EA0457A226F815
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015802 Genomic DNA. Translation: BAA77585.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015802 Genomic DNA. Translation: BAA77585.1.

3D structure databases

ProteinModelPortaliQ9WX61.
SMRiQ9WX61.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00694.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003919. Cell_synth_A.
IPR005150. Cellulose_synth.
IPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR009875. PilZ_domain.
[Graphical view]
PfamiPF03552. Cellulose_synt. 1 hit.
PF00535. Glycos_transf_2. 1 hit.
PF07238. PilZ. 1 hit.
[Graphical view]
PRINTSiPR01439. CELLSNTHASEA.
SUPFAMiSSF53448. SSF53448. 1 hit.
TIGRFAMsiTIGR03030. CelA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBCSA3_KOMXY
AccessioniPrimary (citable) accession number: Q9WX61
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: November 1, 1999
Last modified: November 2, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.