Q9WX61 (BCSA3_GLUXY) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cellulose synthase 1 catalytic subunit [UDP-forming]
EC=2.4.1.12

Gene names

Name:

bcsAI

Organism

Gluconacetobacter xylinus (Acetobacter xylinus)

Taxonomic identifier

28448 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Acetobacteraceae › Gluconacetobacter

Protein attributes

Sequence length	745 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalytic subunit of cellulose synthase. It polymerizes uridine 5'-diphosphate glucose to cellulose. The thick cellulosic mats generated by this enzyme probably provide a specialized protective environment to the bacterium By similarity.
Catalytic activity	UDP-glucose + (1,4-beta-D-glucosyl)(n) = UDP + (1,4-beta-D-glucosyl)(n+1).
Cofactor	Magnesium By similarity.
Enzyme regulation	Activated by bis-(3'-5') cyclic diguanylic acid (c-di-GMP) By similarity.
Pathway	Glycan metabolism; bacterial cellulose biosynthesis.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Potential.
Domain	There are two conserved domains in the globular part of the catalytic subunit: the N-terminal domain (domain A) contains the conserved DXD motif and is possibly involved in catalysis and substrate binding. The C-terminal domain (domain B) contains the QXXRW motif and is present only in processive glycosyl transferases. It could be involved in the processivity function of the enzyme, possibly required for holding the growing glycan chain in the active site.
Sequence similarities	Belongs to the glycosyltransferase 2 family. Contains 1 PilZ domain.

Ontologies

Keywords
Biological process	Cellulose biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	c-di-GMP
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	UDP-glucose metabolic process Inferred from electronic annotation. Source: InterPro cellulose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cellulose synthase (UDP-forming) activity Inferred from electronic annotation. Source: EC cyclic-di-GMP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 745	745	Cellulose synthase 1 catalytic subunit [UDP-forming]		PRO_0000059264
Regions
	Transmembrane	29 – 49	21	Helical; Potential
	Transmembrane	106 – 126	21	Helical; Potential
	Transmembrane	153 – 173	21	Helical; Potential
	Transmembrane	407 – 427	21	Helical; Potential
	Transmembrane	430 – 450	21	Helical; Potential
	Transmembrane	468 – 488	21	Helical; Potential
	Transmembrane	515 – 535	21	Helical; Potential
	Transmembrane	547 – 567	21	Helical; Potential
	Transmembrane	649 – 669	21	Helical; Potential
	Domain	572 – 670	99	PilZ
	Region	147 – 240	94	Catalytic subdomain A
	Region	317 – 377	61	Catalytic subdomain B
Sites
	Active site	189	1	Potential
	Active site	333	1	Potential
	Binding site	236	1	Substrate Potential
	Binding site	238	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9WX61 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 57EA0457A226F815	FASTA	745	83,519
	10 20 30 40 50 60 MSEVQSSAPA ESWFGRFSNK ILSLRGASYV VGALGLCALL AATMVTLSLN EQMIVALVCV 70 80 90 100 110 120 AVFFIVGRRK SRRTQVFLEV LSALVSLRYL TWRLTETLDF DTWTQGILGV TLLLAELYAL 130 140 150 160 170 180 YMLFLSYFQT ISPLHRAPLP LPANPDEWPT VDIFIPTYDE ALSIVRLTVL GALGIDWPPD 190 200 210 220 230 240 KVNVYILDDG RREEFARFAE ACGARYIARP DNAHAKAGNL NYAIKHTTGD HILILDCDHI 250 260 270 280 290 300 PTRAFLQISM GWMVSDSNIA LLQTPHHFYS PDPFQRNLAV GYRTPPEGNL FYGVIQDGND 310 320 330 340 350 360 FWDATFFCGS CAILRRKAIE EIGGFATETV TEDAHTALRM QRKGWSTAYL RIPLASGLAT 370 380 390 400 410 420 ERLITHIGQR MRWARGMIQI FRVDNPMLGS GLKLGQRLCY LSAMTSFFFA IPRVIFLASP 430 440 450 460 470 480 LAFLFFSQNI IAASPLAVGV YAIPHMFHSI ATAAKVNKGW RYSFWSEVYE TVMALFLVRV 490 500 510 520 530 540 TIVTMLFPSK GKFNVTEKGG VLEREEFDLT ATYPNIIFAI IMALGLLRGL YALIFQHLDI 550 560 570 580 590 600 ISERAYALNC IWSVISLIIL MAVISVGRET KQLRQSHRIE AQIPVTVYDY DGNSSHGITE 610 620 630 640 650 660 DVSMGGVAIH LPWREVTPDH PVQVVIHAVL DGEEMNLPAT MIRSAQGKAV FTWSISNIQV 670 680 690 700 710 720 EAAVVRFVFG RADAWLQWNN YEDDRPLRSL WSLILSIKAL FRRKGQMIAH SRPKKKPIAL 730 740 PVERREPTTS QGGQKQEGKI SRAAS

References

[1]

"Cloning of cellulose synthase genes from Acetobacter xylinum JCM 7664: implication of a novel set of cellulose synthase genes." Umeda Y., Hirano A., Ishibashi M., Akiyama H., Onizuka T., Ikeuchi M., Inoue Y. DNA Res. 6:109-115(1999) [PubMed: 10382968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: JCM 7664 / NBRC 13693.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB015802 Genomic DNA. Translation: BAA77585.1.
3D structure databases
ProteinModelPortal	Q9WX61.
ModBase	Search...
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR003919. Cell_synth_A. IPR005150. Cellulose_synth. IPR001173. Glyco_trans_2. IPR009875. PilZ_domain. [Graphical view]
Pfam	PF03552. Cellulose_synt. 1 hit. PF00535. Glycos_transf_2. 1 hit. PF07238. PilZ. 1 hit. [Graphical view]
PRINTS	PR01439. CELLSNTHASEA.
TIGRFAMs	TIGR03030. CelA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BCSA3_GLUXY

Accession

Primary (citable) accession number: Q9WX61

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 26, 2002
Last sequence update:	November 1, 1999
Last modified:	June 28, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program