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Protein

Alpha-ketoglutarate-dependent sulfate ester dioxygenase

Gene

atsK

Organism
Pseudomonas putida (Arthrobacter siderocapsulatus)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxygenolytic cleavage of 2-ethylhexyl sulfate (2-EHS) in the presence of alpha-ketoglutarate to yield 2-ethyl-hexanal and succinate, the decarboxylated form of alpha-ketoglutarate. It can accept a wide range of alpha-keto acids including 2-oxo-valerate, 2-oxo-adipate, 2-oxo-octanoate, 3-methyl-2-oxo-butyrate, oxaloacetate-alpha-ketoadipate, and alpha-ketooctanoate. It can catalyze the cleavage of medium-chain alkyl sulfate esters such as butylsulfate, pentylsulfate, hexylsulfate, heptylsulfate, octylsulfate, nonylsulfate, decylsulfate and sodium dodecyl sulfate (SDS).1 Publication

Catalytic activityi

2-ethylhexyl sulfate + alpha-ketoglutarate + O2 = 2-ethyl-hexanal + succinate + CO2 + sulfate.1 Publication

Cofactori

Fe2+3 Publications

Enzyme regulationi

Strongly stimulated by ascorbate.1 Publication

Kineticsi

  1. KM=34 mM for SDS (at pH 7 and 30 degrees Celsius)1 Publication
  2. KM=40 mM for hexylsulfate (at pH 7 and 30 degrees Celsius)1 Publication
  3. KM=60 mM for nonylsulfate (at pH 7 and 30 degrees Celsius)1 Publication
  4. KM=66 mM for 2-EHS (at pH 7 and 30 degrees Celsius)1 Publication
  5. KM=97 mM for decylsulfate (at pH 7 and 30 degrees Celsius)1 Publication
  6. KM=140 mM for alpha-ketoglutarate (at pH 7 and 30 degrees Celsius)1 Publication
  7. KM=271 mM for heptylsulfate (at pH 7 and 30 degrees Celsius)1 Publication
  8. KM=437 mM for octylsulfate (at pH 7 and 30 degrees Celsius)1 Publication

    pH dependencei

    Optimum pH is 7.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei81Substrate1 Publication1
    Metal bindingi108Iron2 Publications1
    Metal bindingi110Iron2 Publications1
    Binding sitei111Substrate; via amide nitrogen1 Publication1
    Binding sitei1352-oxoglutarate2 Publications1
    Metal bindingi264Iron2 Publications1
    Binding sitei2752-oxoglutarate2 Publications1
    Binding sitei2792-oxoglutarate2 Publications1

    GO - Molecular functioni

    Complete GO annotation...

    Keywords - Molecular functioni

    Dioxygenase, Oxidoreductase

    Keywords - Ligandi

    Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-ketoglutarate-dependent sulfate ester dioxygenase1 Publication (EC:1.14.11.-1 Publication)
    Alternative name(s):
    Alkylsulfatase1 Publication
    Type II alkyl sulfatase1 Publication
    Gene namesi
    Name:atsK1 Publication
    OrganismiPseudomonas putida (Arthrobacter siderocapsulatus)
    Taxonomic identifieri303 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004324701 – 301Alpha-ketoglutarate-dependent sulfate ester dioxygenaseAdd BLAST301

    Interactioni

    Subunit structurei

    Homotetramer.3 Publications

    Structurei

    Secondary structure

    1301
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi16 – 24Combined sources9
    Beta strandi26 – 30Combined sources5
    Helixi39 – 52Combined sources14
    Beta strandi53 – 57Combined sources5
    Helixi65 – 73Combined sources9
    Beta strandi78 – 81Combined sources4
    Beta strandi94 – 96Combined sources3
    Turni110 – 113Combined sources4
    Beta strandi114 – 116Combined sources3
    Beta strandi119 – 127Combined sources9
    Beta strandi135 – 139Combined sources5
    Helixi140 – 145Combined sources6
    Helixi149 – 157Combined sources9
    Beta strandi159 – 163Combined sources5
    Beta strandi193 – 201Combined sources9
    Turni203 – 205Combined sources3
    Beta strandi208 – 210Combined sources3
    Beta strandi216 – 219Combined sources4
    Helixi224 – 238Combined sources15
    Helixi241 – 243Combined sources3
    Beta strandi244 – 247Combined sources4
    Beta strandi254 – 258Combined sources5
    Beta strandi261 – 266Combined sources6
    Beta strandi276 – 282Combined sources7
    Beta strandi296 – 299Combined sources4

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1OIHX-ray1.89A/B/C/D1-301[»]
    1OIIX-ray2.19A/B/C/D1-301[»]
    1OIJX-ray2.10A/B/C/D1-301[»]
    1OIKX-ray2.06A/D1-301[»]
    1VZ4X-ray2.50A/D1-301[»]
    1VZ5X-ray2.15A/B/C/D1-301[»]
    ProteinModelPortaliQ9WWU5.
    SMRiQ9WWU5.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9WWU5.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the TfdA dioxygenase family.Curated

    Family and domain databases

    InterProiIPR003819. TauD/TfdA-like.
    [Graphical view]
    PfamiPF02668. TauD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9WWU5-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSNAALATAP HALELDVHPV AGRIGAEIRG VKLSPDLDAA TVEAIQAALV
    60 70 80 90 100
    RHKVIFFRGQ THLDDQSQEG FAKLLGEPVA HPTVPVVDGT RYLLQLDGAQ
    110 120 130 140 150
    GQRANSWHTD VTFVEAYPKA SILRSVVAPA SGGDTVWANT AAAYQELPEP
    160 170 180 190 200
    LRELADKLWA VHSNEYDYAS LKPDIDPAKL ERHRKVFTST VYETEHPVVR
    210 220 230 240 250
    VHPISGERAL QLGHFVKRIK GYSLADSQHL FAVLQGHVTR LENTVRWRWE
    260 270 280 290 300
    AGDVAIWDNR ATQHYAVDDY GTQPRIVRRV TLAGEVPVGV DGQLSRTTRK

    G
    Length:301
    Mass (Da):33,201
    Last modified:November 1, 1999 - v1
    Checksum:iADB169D98E56C71A
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF126201 Genomic DNA. Translation: AAD31784.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF126201 Genomic DNA. Translation: AAD31784.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1OIHX-ray1.89A/B/C/D1-301[»]
    1OIIX-ray2.19A/B/C/D1-301[»]
    1OIJX-ray2.10A/B/C/D1-301[»]
    1OIKX-ray2.06A/D1-301[»]
    1VZ4X-ray2.50A/D1-301[»]
    1VZ5X-ray2.15A/B/C/D1-301[»]
    ProteinModelPortaliQ9WWU5.
    SMRiQ9WWU5.
    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9WWU5.

    Family and domain databases

    InterProiIPR003819. TauD/TfdA-like.
    [Graphical view]
    PfamiPF02668. TauD. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiATSK_PSEPU
    AccessioniPrimary (citable) accession number: Q9WWU5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 2015
    Last sequence update: November 1, 1999
    Last modified: November 2, 2016
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.