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Q9WVT0 (GP116_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable G-protein coupled receptor 116
Alternative name(s):
G-protein coupled hepta-helical receptor Ig-hepta
Gene names
Name:Gpr116
Synonyms:Gprhep
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length1349 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May have a role in the regulation of acid-base balance.

Subunit structure

Exists as disulfide-linked dimers at the cell surface.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Highly expressed in the lung and to a much lesser extent in the kidney and heart. Dense localization in alveolar walls of the lung and in the intercalated cells of the collecting duct of the kidney. Ref.1

Developmental stage

Strongly induced postnatally.

Post-translational modification

Proteolytically cleaved into 2 highly conserved sites: one in the SEA domain and the other in the stalk domain region preceding the first transmembrane. The later 2 subunits, the extracellular subunit and the seven-transmembrane subunit, remain tightly associated and non-covalently linked.

Sequence similarities

Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.

Contains 1 GPS domain.

Contains 3 Ig-like (immunoglobulin-like) domains.

Contains 1 SEA domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 13491325Probable G-protein coupled receptor 116
PRO_0000012897

Regions

Topological domain25 – 1016992Extracellular Potential
Transmembrane1017 – 103620Helical; Name=1; Potential
Topological domain1037 – 105519Cytoplasmic Potential
Transmembrane1056 – 107823Helical; Name=2; Potential
Topological domain1079 – 109719Extracellular Potential
Transmembrane1098 – 112023Helical; Name=3; Potential
Topological domain1121 – 113111Cytoplasmic Potential
Transmembrane1132 – 115423Helical; Name=4; Potential
Topological domain1155 – 117319Extracellular Potential
Transmembrane1174 – 119623Helical; Name=5; Potential
Topological domain1197 – 121620Cytoplasmic Potential
Transmembrane1217 – 123923Helical; Name=6; Potential
Topological domain1240 – 12489Extracellular Potential
Transmembrane1249 – 127123Helical; Name=7; Potential
Topological domain1272 – 134978Cytoplasmic Potential
Domain159 – 279121SEA
Domain268 – 36699Ig-like 1
Domain367 – 46498Ig-like 2
Domain469 – 55991Ig-like 3
Domain951 – 100555GPS

Sites

Site223 – 2242Cleavage
Site993 – 9942Cleavage

Amino acid modifications

Glycosylation731N-linked (GlcNAc...) Potential
Glycosylation941N-linked (GlcNAc...) Potential
Glycosylation1851N-linked (GlcNAc...) Potential
Glycosylation2541N-linked (GlcNAc...) Potential
Glycosylation2701N-linked (GlcNAc...) Potential
Glycosylation2861N-linked (GlcNAc...) Potential
Glycosylation2991N-linked (GlcNAc...) Potential
Glycosylation3261N-linked (GlcNAc...) Potential
Glycosylation3371N-linked (GlcNAc...) Potential
Glycosylation3491N-linked (GlcNAc...) Potential
Glycosylation3961N-linked (GlcNAc...) Potential
Glycosylation4701N-linked (GlcNAc...) Potential
Glycosylation5031N-linked (GlcNAc...) Potential
Glycosylation5381N-linked (GlcNAc...) Potential
Glycosylation6491N-linked (GlcNAc...) Potential
Glycosylation6661N-linked (GlcNAc...) Potential
Glycosylation8201N-linked (GlcNAc...) Potential
Glycosylation9581N-linked (GlcNAc...) Potential
Glycosylation9631N-linked (GlcNAc...) Potential
Glycosylation10851N-linked (GlcNAc...) Potential

Experimental info

Mutagenesis9941T → A: Abolishes cleavage. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9WVT0 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 104CF1D9A35B1409

FASTA1,349149,446
        10         20         30         40         50         60 
MKSSRTVTLY FVLIVICSSE ATWSRPAEPI VHPLILQEHE LAGEELLRPK RAVAVGGPVA 

        70         80         90        100        110        120 
EEYTVDVEIS FENVSFLESI RAHLNSLRFP VQGNGTDILS MAMTTVCTPT GNDLLCFCEK 

       130        140        150        160        170        180 
GYQWPEERCL SSLTCQEHDS ALPGRYCNCL KGLPPQGPFC QLPETYITLK IKVRLNIGFQ 

       190        200        210        220        230        240 
EDLENTSSAL YRSYKTDLER AFRAGYRTLP GFRSVTVTQF TKGSVVVDYI VEVASAPLPG 

       250        260        270        280        290        300 
SIHKANEQVI QNLNQTYKMD YNSFQGTPSN ETKFTVTPEF IFEGDNVTLE CESEFVSSNT 

       310        320        330        340        350        360 
SWFYGEKRSD IQNSDKFSIH TSIINNISLV TRLTIFNFTQ HDAGLYGCNV TLDIFEYGTV 

       370        380        390        400        410        420 
RKLDVTPIRI LAKEERKVVC DNNPISLNCC SENIANWSRI EWKQEGKINI EGTPETDLES 

       430        440        450        460        470        480 
SCSTYTLKAD GTQCPSGSSG TTVIYTCEFV SVYGAKGSKN IAVTFTSVAN LTITPDPISV 

       490        500        510        520        530        540 
SEGQSFSITC LSDVSSFDEV YWNTSAGIKI HPRFYTMRRY RDGAESVLTV KTSTREWNGT 

       550        560        570        580        590        600 
YHCIFRYKNS YSIATKDVTV HPLPLESDIM MDPLEASGLC TSSHQFKCCI EENDGEEYIV 

       610        620        630        640        650        660 
TFHVDSSSFP AEREVIGKQA CYTYSLPGKL PSRCPKDIDV FCHFTNAANS SVRSPSMKLT 

       670        680        690        700        710        720 
LVPGKNITCQ DPIIGIGEPG KVIQKLCQFA GVSRSPGQTI GGTVTYKCVG SQWKEETRAC 

       730        740        750        760        770        780 
ISAPINGLLQ LAKALIKSPS QDQKLPKYLR DLSVSTGKEE QDIRSSPGSL GAIISILDLL 

       790        800        810        820        830        840 
STVPTQVNSE MMRDILATIN VILDKSTLNS WEKLLQQQSN QSSQFLQSVE RFSKALELGD 

       850        860        870        880        890        900 
STPPFLFHPN VQMKSMVIKR GHAQMYQQKF VFTDSDLWGD VAIDECQLGS LQPDSSIVTV 

       910        920        930        940        950        960 
AFPTLKAILA QDGQRKTPSN SLVMTTTVSH NIVKPFRISM TFKNNHRSGG KPQCVFWNFS 

       970        980        990       1000       1010       1020 
LANNTGGWDS SGCTVEDDGR DNRDRVFCKC NHLTSFSILM SPDSPDPGSL LKILLDIISY 

      1030       1040       1050       1060       1070       1080 
IGLGFSIVSL AACLVVEAMV WKSVTKNRTS YMRHICIVNI ALCLLIADIW FIVAGAIHDG 

      1090       1100       1110       1120       1130       1140 
HYPLNETACV AATFFIHFFY LSVFFWMLTL GLMLFYRLIF ILHDASKSTQ KAIAFSLGYG 

      1150       1160       1170       1180       1190       1200 
CPLIISSITV GVTQPQEVYM RKNACWLNWE DTRALLAFAI PALIIVVVNV SITVVVITKI 

      1210       1220       1230       1240       1250       1260 
LRPSVGDKPG KQEKSSLFQI SKSIGVLTPL LGLTWGFGLA TVIQGSNAVF HIIFTLLNAF 

      1270       1280       1290       1300       1310       1320 
QGLFILLFGC LWDQKVQEAL LHKFSLSRWS SQHSKSTSLG SSTPVFSMSS PISRRFNNLF 

      1330       1340 
GKTGTYNVST PETTSSSVEN SSSAYSLLN

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References

[1]"Ig-hepta, a novel member of the G protein-coupled hepta-helical receptor (GPCR) family that has immunoglobulin-like repeats in a long N-terminal extracellular domain and defines a new subfamily of GPCRs."
Abe J., Suzuki H., Notoya M., Yamamoto T., Hirose S.
J. Biol. Chem. 274:19957-19964(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Tissue: Lung.
[2]"Cleavage of Ig-Hepta at a 'SEA' module and at a conserved G protein-coupled receptor proteolytic site."
Abe J., Fukuzawa T., Hirose S.
J. Biol. Chem. 277:23391-23398(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 224-233 AND 994-1003, MUTAGENESIS OF THR-994.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB019120 mRNA. Translation: BAA82518.1.
IPIIPI00205333.
RefSeqNP_620810.1. NM_139110.1.
UniGeneRn.25073.

3D structure databases

ProteinModelPortalQ9WVT0.
ModBaseSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000015223.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteQ9WVT0.

Proteomic databases

PaxDbQ9WVT0.
PRIDEQ9WVT0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000015223; ENSRNOP00000015223; ENSRNOG00000011154.
GeneID245977.
KEGGrno:245977.
UCSCRGD:621679. rat.

Organism-specific databases

CTD221395.
RGD621679. Gpr116.

Phylogenomic databases

eggNOGNOG235397.
GeneTreeENSGT00610000085899.
HOGENOMHOG000112764.
HOVERGENHBG051772.
InParanoidQ9WVT0.
KOK08458.
OMASTPVFSM.

Gene expression databases

ArrayExpressQ9WVT0.
GenevestigatorQ9WVT0.
GermOnlineENSRNOG00000011154. Rattus norvegicus.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
InterProIPR017981. GPCR_2-like.
IPR008078. GPCR_2_Ig-hepta_rcpt.
IPR000832. GPCR_2_secretin-like.
IPR017983. GPCR_2_secretin-like_CS.
IPR000203. GPS_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013151. Immunoglobulin.
IPR000082. SEA.
[Graphical view]
PfamPF00002. 7tm_2. 1 hit.
PF01825. GPS. 1 hit.
PF00047. ig. 1 hit.
PF01390. SEA. 1 hit.
[Graphical view]
PRINTSPR00249. GPCRSECRETIN.
PR01695. IGHEPTARCPTR.
SMARTSM00303. GPS. 1 hit.
SM00409. IG. 2 hits.
SM00200. SEA. 1 hit.
[Graphical view]
PROSITEPS00649. G_PROTEIN_RECEP_F2_1. False negative.
PS00650. G_PROTEIN_RECEP_F2_2. 1 hit.
PS50227. G_PROTEIN_RECEP_F2_3. False negative.
PS50261. G_PROTEIN_RECEP_F2_4. 1 hit.
PS50221. GPS. 1 hit.
PS50835. IG_LIKE. 3 hits.
PS50024. SEA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio623258.

Entry information

Entry nameGP116_RAT
AccessionPrimary (citable) accession number: Q9WVT0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: November 1, 1999
Last modified: April 3, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents