Q9WVS4 (MOK_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
MAPK/MAK/MRK overlapping kinase
EC=2.7.11.22

Alternative name(s):
MOK protein kinase
Serine/threonine kinase 30

Gene names

Name:	Mok
Synonyms:	Rage, Stk30

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Able to phosphorylate several exogenous substrates and to undergo autophosphorylation. Ref.1
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Phosphorylation appears to increase the enzymatic activity.
Subcellular location	Cytoplasm Ref.1.
Tissue specificity	Highly expressed in testis, and less in kidney, brain and lung.
Post-translational modification	Autophosphorylated. Ref.1
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9WVS4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9WVS4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-92: Missing.
     93-95: IRG → MKR
     232-289: Missing.
     328-420: KQSLRHEEGH...PTINRKGGEY → V

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 420	420	MAPK/MAK/MRK overlapping kinase		PRO_0000086342
Regions
	Domain	4 – 285	282	Protein kinase
	Nucleotide binding	10 – 18	9	ATP By similarity
Sites
	Active site	128	1	Proton acceptor By similarity
	Binding site	33	1	ATP By similarity
Natural variations
	Alternative sequence	1 – 92	92	Missing in isoform 2.		VSP_009147
	Alternative sequence	93 – 95	3	IRG → MKR in isoform 2.		VSP_009148
	Alternative sequence	232 – 289	58	Missing in isoform 2.		VSP_009149
	Alternative sequence	328 – 420	93	KQSLR…KGGEY → V in isoform 2.		VSP_009150
Experimental info
	Mutagenesis	159	1	T → A: Loss of activity; when associated with F-161. Ref.1
	Mutagenesis	161	1	Y → F: Loss of activity; when associated with A-159. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 1C637E1ECB33C998	FASTA	420	48,065
	10 20 30 40 50 60 MKNYKAIGKI GEGTFSEVMK MQSLRDGNYY ACKQMKQHFE SIEQVNSLRE IQALRRLNPH 70 80 90 100 110 120 PNILALHEVV FDRKSGSLAL ICELMDMNIY ELIRGRRHPL SEKKIMLYMY QLCKSLDHMH 130 140 150 160 170 180 RNGIFHRDVK PENILVKQDV LKLGDFGSCR SVYSKQPYTE YISTRWYRAP ECLLTDGFYT 190 200 210 220 230 240 YKMDLWSAGC VFYEIASLQP LFPGVNELDQ ISKIHDVIGT PCQKTLTKFK QSRAMSFDFP 250 260 270 280 290 300 FKKGSGIPLL TANLSPQCLS LLHAMVAYDP DERIAAHQAL QHPYFQVQRA AETQTLAKHR 310 320 330 340 350 360 RAFCPKFSMV PESSSHNWSF SQEGRKQKQS LRHEEGHARR QGPTSLMELP KLRLSGMTKL 370 380 390 400 410 420 SSCSSPALRS VLGTGANGKV PVLRPLKCAA VNKKTDTQKD IKPHLKHYHL PTINRKGGEY
	Isoform 2 [UniParc]. Checksum: 95E8F3A2221673FB	FASTA	178	20,864
	10 20 30 40 50 60 MKRRRHPLSE KKIMLYMYQL CKSLDHMHRN GIFHRDVKPE NILVKQDVLK LGDFGSCRSV 70 80 90 100 110 120 YSKQPYTEYI STRWYRAPEC LLTDGFYTYK MDLWSAGCVF YEIASLQPLF PGVNELDQIS 130 140 150 160 170 KIHDVIGTPC QKTLTKFKQA AETQTLAKHR RAFCPKFSMV PESSSHNWSF SQEGRKQV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of a novel member of the MAP kinase superfamily." Miyata Y., Akashi M., Nishida E. Genes Cells 4:299-309(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, AUTOPHOSPHORYLATION, MUTAGENESIS OF THR-159 AND TYR-161. Strain: C57BL/6. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6. Tissue: Retina.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB022695 mRNA. Translation: BAA81689.1. BC037614 mRNA. Translation: AAH37614.1.
IPI	IPI00267984. IPI00271851.
RefSeq	NP_036103.1. NM_011973.2.
UniGene	Mm.140948.
3D structure databases
ProteinModelPortal	Q9WVS4.
SMR	Q9WVS4. Positions 1-332.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9WVS4. 1 interaction.
PTM databases
PhosphoSite	Q9WVS4.
Proteomic databases
PRIDE	Q9WVS4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000070565; ENSMUSP00000068904; ENSMUSG00000056458.
GeneID	26448.
KEGG	mmu:26448.
UCSC	uc007pbv.1. mouse. uc007pbx.1. mouse.
Organism-specific databases
CTD	26448.
MGI	MGI:1336881. Stk30.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00650000093283.
HOGENOM	HOG000233024.
HOVERGEN	HBG106271.
InParanoid	Q9WVS4.
KO	K08830.
OMA	ILMLHEV.
Gene expression databases
ArrayExpress	Q9WVS4.
Bgee	Q9WVS4.
CleanEx	MM_RAGE.
Genevestigator	Q9WVS4.
GermOnline	ENSMUSG00000056458. Mus musculus.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	304549.
SOURCE	Search...

Entry information

Entry name

MOK_MOUSE

Accession

Primary (citable) accession number: Q9WVS4
Secondary accession number(s): Q8CFU4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	November 1, 1999
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program