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Protein

MAPK/MAK/MRK overlapping kinase

Gene

Mok

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Able to phosphorylate several exogenous substrates and to undergo autophosphorylation.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Phosphorylation appears to increase the enzymatic activity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331ATPPROSITE-ProRule annotation
Active sitei128 – 1281Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189ATPPROSITE-ProRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
MAPK/MAK/MRK overlapping kinase (EC:2.7.11.22)
Alternative name(s):
MOK protein kinase
Serine/threonine kinase 30
Gene namesi
Name:Mok
Synonyms:Rage, Stk30
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1336881. Mok.

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi159 – 1591T → A: Loss of activity; when associated with F-161. 1 Publication
Mutagenesisi161 – 1611Y → F: Loss of activity; when associated with A-159. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 420420MAPK/MAK/MRK overlapping kinasePRO_0000086342Add
BLAST

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ9WVS4.

PTM databases

PhosphoSiteiQ9WVS4.

Expressioni

Tissue specificityi

Highly expressed in testis, and less in kidney, brain and lung.

Gene expression databases

BgeeiQ9WVS4.
CleanExiMM_RAGE.
ExpressionAtlasiQ9WVS4. baseline and differential.
GenevestigatoriQ9WVS4.

Interactioni

Protein-protein interaction databases

IntActiQ9WVS4. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9WVS4.
SMRiQ9WVS4. Positions 2-327.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 285282Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00650000093283.
HOGENOMiHOG000233024.
HOVERGENiHBG106271.
InParanoidiQ9WVS4.
KOiK08830.
OMAiILMLHEV.
PhylomeDBiQ9WVS4.
TreeFamiTF328769.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9WVS4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKNYKAIGKI GEGTFSEVMK MQSLRDGNYY ACKQMKQHFE SIEQVNSLRE
60 70 80 90 100
IQALRRLNPH PNILALHEVV FDRKSGSLAL ICELMDMNIY ELIRGRRHPL
110 120 130 140 150
SEKKIMLYMY QLCKSLDHMH RNGIFHRDVK PENILVKQDV LKLGDFGSCR
160 170 180 190 200
SVYSKQPYTE YISTRWYRAP ECLLTDGFYT YKMDLWSAGC VFYEIASLQP
210 220 230 240 250
LFPGVNELDQ ISKIHDVIGT PCQKTLTKFK QSRAMSFDFP FKKGSGIPLL
260 270 280 290 300
TANLSPQCLS LLHAMVAYDP DERIAAHQAL QHPYFQVQRA AETQTLAKHR
310 320 330 340 350
RAFCPKFSMV PESSSHNWSF SQEGRKQKQS LRHEEGHARR QGPTSLMELP
360 370 380 390 400
KLRLSGMTKL SSCSSPALRS VLGTGANGKV PVLRPLKCAA VNKKTDTQKD
410 420
IKPHLKHYHL PTINRKGGEY
Length:420
Mass (Da):48,065
Last modified:November 1, 1999 - v1
Checksum:i1C637E1ECB33C998
GO
Isoform 2 (identifier: Q9WVS4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-92: Missing.
     93-95: IRG → MKR
     232-289: Missing.
     328-420: KQSLRHEEGH...PTINRKGGEY → V

Note: No experimental confirmation available.

Show »
Length:178
Mass (Da):20,864
Checksum:i95E8F3A2221673FB
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 9292Missing in isoform 2. 1 PublicationVSP_009147Add
BLAST
Alternative sequencei93 – 953IRG → MKR in isoform 2. 1 PublicationVSP_009148
Alternative sequencei232 – 28958Missing in isoform 2. 1 PublicationVSP_009149Add
BLAST
Alternative sequencei328 – 42093KQSLR…KGGEY → V in isoform 2. 1 PublicationVSP_009150Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB022695 mRNA. Translation: BAA81689.1.
BC037614 mRNA. Translation: AAH37614.1.
CCDSiCCDS49176.1. [Q9WVS4-1]
RefSeqiNP_036103.1. NM_011973.2. [Q9WVS4-1]
UniGeneiMm.140948.

Genome annotation databases

EnsembliENSMUST00000070565; ENSMUSP00000068904; ENSMUSG00000056458. [Q9WVS4-1]
GeneIDi26448.
KEGGimmu:26448.
UCSCiuc007pbv.1. mouse. [Q9WVS4-1]
uc007pbx.1. mouse. [Q9WVS4-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB022695 mRNA. Translation: BAA81689.1.
BC037614 mRNA. Translation: AAH37614.1.
CCDSiCCDS49176.1. [Q9WVS4-1]
RefSeqiNP_036103.1. NM_011973.2. [Q9WVS4-1]
UniGeneiMm.140948.

3D structure databases

ProteinModelPortaliQ9WVS4.
SMRiQ9WVS4. Positions 2-327.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9WVS4. 1 interaction.

PTM databases

PhosphoSiteiQ9WVS4.

Proteomic databases

PRIDEiQ9WVS4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000070565; ENSMUSP00000068904; ENSMUSG00000056458. [Q9WVS4-1]
GeneIDi26448.
KEGGimmu:26448.
UCSCiuc007pbv.1. mouse. [Q9WVS4-1]
uc007pbx.1. mouse. [Q9WVS4-2]

Organism-specific databases

CTDi5891.
MGIiMGI:1336881. Mok.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00650000093283.
HOGENOMiHOG000233024.
HOVERGENiHBG106271.
InParanoidiQ9WVS4.
KOiK08830.
OMAiILMLHEV.
PhylomeDBiQ9WVS4.
TreeFamiTF328769.

Miscellaneous databases

NextBioi304549.
PROiQ9WVS4.
SOURCEiSearch...

Gene expression databases

BgeeiQ9WVS4.
CleanExiMM_RAGE.
ExpressionAtlasiQ9WVS4. baseline and differential.
GenevestigatoriQ9WVS4.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of a novel member of the MAP kinase superfamily."
    Miyata Y., Akashi M., Nishida E.
    Genes Cells 4:299-309(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, AUTOPHOSPHORYLATION, MUTAGENESIS OF THR-159 AND TYR-161.
    Strain: C57BL/6.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6.
    Tissue: Retina.

Entry informationi

Entry nameiMOK_MOUSE
AccessioniPrimary (citable) accession number: Q9WVS4
Secondary accession number(s): Q8CFU4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: November 1, 1999
Last modified: May 27, 2015
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.