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Protein

MAPK/MAK/MRK overlapping kinase

Gene

Mok

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Able to phosphorylate several exogenous substrates and to undergo autophosphorylation. Negatively regulates cilium length in a cAMP and mTORC1 signaling-dependent manner (PubMed:25243405).2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Phosphorylation appears to increase the enzymatic activity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33ATPPROSITE-ProRule annotation1
Active sitei128Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 18ATPPROSITE-ProRule annotation9

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
MAPK/MAK/MRK overlapping kinase (EC:2.7.11.22)
Alternative name(s):
MOK protein kinase
Serine/threonine kinase 30
Gene namesi
Name:Mok
Synonyms:Rage, Stk30
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1336881. Mok.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Cell projectioncilium 1 Publication
  • Nucleus 1 Publication

GO - Cellular componenti

  • ciliary base Source: UniProtKB
  • cilium Source: UniProtKB
  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi159T → A: Loss of activity; when associated with F-161. 1 Publication1
Mutagenesisi161Y → F: Loss of activity; when associated with A-159. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000863421 – 420MAPK/MAK/MRK overlapping kinaseAdd BLAST420

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9WVS4.
PRIDEiQ9WVS4.

PTM databases

iPTMnetiQ9WVS4.
PhosphoSitePlusiQ9WVS4.

Expressioni

Tissue specificityi

Highly expressed in testis, and less in kidney, brain and lung.

Gene expression databases

BgeeiENSMUSG00000056458.
CleanExiMM_RAGE.
ExpressionAtlasiQ9WVS4. baseline and differential.
GenevisibleiQ9WVS4. MM.

Interactioni

Protein-protein interaction databases

IntActiQ9WVS4. 1 interactor.
STRINGi10090.ENSMUSP00000068904.

Structurei

3D structure databases

ProteinModelPortaliQ9WVS4.
SMRiQ9WVS4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 285Protein kinasePROSITE-ProRule annotationAdd BLAST282

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0661. Eukaryota.
ENOG410XPBB. LUCA.
GeneTreeiENSGT00650000093283.
HOGENOMiHOG000233024.
HOVERGENiHBG106271.
InParanoidiQ9WVS4.
KOiK08830.
OMAiTQKDIKP.
OrthoDBiEOG091G0DU3.
PhylomeDBiQ9WVS4.
TreeFamiTF328769.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9WVS4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKNYKAIGKI GEGTFSEVMK MQSLRDGNYY ACKQMKQHFE SIEQVNSLRE
60 70 80 90 100
IQALRRLNPH PNILALHEVV FDRKSGSLAL ICELMDMNIY ELIRGRRHPL
110 120 130 140 150
SEKKIMLYMY QLCKSLDHMH RNGIFHRDVK PENILVKQDV LKLGDFGSCR
160 170 180 190 200
SVYSKQPYTE YISTRWYRAP ECLLTDGFYT YKMDLWSAGC VFYEIASLQP
210 220 230 240 250
LFPGVNELDQ ISKIHDVIGT PCQKTLTKFK QSRAMSFDFP FKKGSGIPLL
260 270 280 290 300
TANLSPQCLS LLHAMVAYDP DERIAAHQAL QHPYFQVQRA AETQTLAKHR
310 320 330 340 350
RAFCPKFSMV PESSSHNWSF SQEGRKQKQS LRHEEGHARR QGPTSLMELP
360 370 380 390 400
KLRLSGMTKL SSCSSPALRS VLGTGANGKV PVLRPLKCAA VNKKTDTQKD
410 420
IKPHLKHYHL PTINRKGGEY
Length:420
Mass (Da):48,065
Last modified:November 1, 1999 - v1
Checksum:i1C637E1ECB33C998
GO
Isoform 2 (identifier: Q9WVS4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-92: Missing.
     93-95: IRG → MKR
     232-289: Missing.
     328-420: KQSLRHEEGH...PTINRKGGEY → V

Note: No experimental confirmation available.
Show »
Length:178
Mass (Da):20,864
Checksum:i95E8F3A2221673FB
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0091471 – 92Missing in isoform 2. 1 PublicationAdd BLAST92
Alternative sequenceiVSP_00914893 – 95IRG → MKR in isoform 2. 1 Publication3
Alternative sequenceiVSP_009149232 – 289Missing in isoform 2. 1 PublicationAdd BLAST58
Alternative sequenceiVSP_009150328 – 420KQSLR…KGGEY → V in isoform 2. 1 PublicationAdd BLAST93

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB022695 mRNA. Translation: BAA81689.1.
BC037614 mRNA. Translation: AAH37614.1.
CCDSiCCDS49176.1. [Q9WVS4-1]
RefSeqiNP_036103.1. NM_011973.2. [Q9WVS4-1]
UniGeneiMm.140948.

Genome annotation databases

EnsembliENSMUST00000070565; ENSMUSP00000068904; ENSMUSG00000056458. [Q9WVS4-1]
GeneIDi26448.
KEGGimmu:26448.
UCSCiuc007pbv.1. mouse. [Q9WVS4-1]
uc007pbx.1. mouse. [Q9WVS4-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB022695 mRNA. Translation: BAA81689.1.
BC037614 mRNA. Translation: AAH37614.1.
CCDSiCCDS49176.1. [Q9WVS4-1]
RefSeqiNP_036103.1. NM_011973.2. [Q9WVS4-1]
UniGeneiMm.140948.

3D structure databases

ProteinModelPortaliQ9WVS4.
SMRiQ9WVS4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9WVS4. 1 interactor.
STRINGi10090.ENSMUSP00000068904.

PTM databases

iPTMnetiQ9WVS4.
PhosphoSitePlusiQ9WVS4.

Proteomic databases

PaxDbiQ9WVS4.
PRIDEiQ9WVS4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000070565; ENSMUSP00000068904; ENSMUSG00000056458. [Q9WVS4-1]
GeneIDi26448.
KEGGimmu:26448.
UCSCiuc007pbv.1. mouse. [Q9WVS4-1]
uc007pbx.1. mouse. [Q9WVS4-2]

Organism-specific databases

CTDi5891.
MGIiMGI:1336881. Mok.

Phylogenomic databases

eggNOGiKOG0661. Eukaryota.
ENOG410XPBB. LUCA.
GeneTreeiENSGT00650000093283.
HOGENOMiHOG000233024.
HOVERGENiHBG106271.
InParanoidiQ9WVS4.
KOiK08830.
OMAiTQKDIKP.
OrthoDBiEOG091G0DU3.
PhylomeDBiQ9WVS4.
TreeFamiTF328769.

Miscellaneous databases

PROiQ9WVS4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000056458.
CleanExiMM_RAGE.
ExpressionAtlasiQ9WVS4. baseline and differential.
GenevisibleiQ9WVS4. MM.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMOK_MOUSE
AccessioniPrimary (citable) accession number: Q9WVS4
Secondary accession number(s): Q8CFU4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: November 1, 1999
Last modified: November 2, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.