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Q9WVS4 (MOK_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
MAPK/MAK/MRK overlapping kinase

EC=2.7.11.22
Alternative name(s):
MOK protein kinase
Serine/threonine kinase 30
Gene names
Name:Mok
Synonyms:Rage, Stk30
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length420 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Able to phosphorylate several exogenous substrates and to undergo autophosphorylation. Ref.1

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Phosphorylation appears to increase the enzymatic activity.

Subcellular location

Cytoplasm Ref.1.

Tissue specificity

Highly expressed in testis, and less in kidney, brain and lung.

Post-translational modification

Autophosphorylated. Ref.1

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Cellular componentCytoplasm
   Coding sequence diversityAlternative splicing
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

cyclin-dependent protein serine/threonine kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9WVS4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9WVS4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-92: Missing.
     93-95: IRG → MKR
     232-289: Missing.
     328-420: KQSLRHEEGH...PTINRKGGEY → V
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 420420MAPK/MAK/MRK overlapping kinase
PRO_0000086342

Regions

Domain4 – 285282Protein kinase
Nucleotide binding10 – 189ATP By similarity

Sites

Active site1281Proton acceptor By similarity
Binding site331ATP By similarity

Natural variations

Alternative sequence1 – 9292Missing in isoform 2.
VSP_009147
Alternative sequence93 – 953IRG → MKR in isoform 2.
VSP_009148
Alternative sequence232 – 28958Missing in isoform 2.
VSP_009149
Alternative sequence328 – 42093KQSLR…KGGEY → V in isoform 2.
VSP_009150

Experimental info

Mutagenesis1591T → A: Loss of activity; when associated with F-161. Ref.1
Mutagenesis1611Y → F: Loss of activity; when associated with A-159. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 1C637E1ECB33C998

FASTA42048,065
        10         20         30         40         50         60 
MKNYKAIGKI GEGTFSEVMK MQSLRDGNYY ACKQMKQHFE SIEQVNSLRE IQALRRLNPH 

        70         80         90        100        110        120 
PNILALHEVV FDRKSGSLAL ICELMDMNIY ELIRGRRHPL SEKKIMLYMY QLCKSLDHMH 

       130        140        150        160        170        180 
RNGIFHRDVK PENILVKQDV LKLGDFGSCR SVYSKQPYTE YISTRWYRAP ECLLTDGFYT 

       190        200        210        220        230        240 
YKMDLWSAGC VFYEIASLQP LFPGVNELDQ ISKIHDVIGT PCQKTLTKFK QSRAMSFDFP 

       250        260        270        280        290        300 
FKKGSGIPLL TANLSPQCLS LLHAMVAYDP DERIAAHQAL QHPYFQVQRA AETQTLAKHR 

       310        320        330        340        350        360 
RAFCPKFSMV PESSSHNWSF SQEGRKQKQS LRHEEGHARR QGPTSLMELP KLRLSGMTKL 

       370        380        390        400        410        420 
SSCSSPALRS VLGTGANGKV PVLRPLKCAA VNKKTDTQKD IKPHLKHYHL PTINRKGGEY 

« Hide

Isoform 2 [UniParc].

Checksum: 95E8F3A2221673FB
Show »

FASTA17820,864

References

« Hide 'large scale' references
[1]"Molecular cloning and characterization of a novel member of the MAP kinase superfamily."
Miyata Y., Akashi M., Nishida E.
Genes Cells 4:299-309(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, AUTOPHOSPHORYLATION, MUTAGENESIS OF THR-159 AND TYR-161.
Strain: C57BL/6.
Tissue: Brain.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6.
Tissue: Retina.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB022695 mRNA. Translation: BAA81689.1.
BC037614 mRNA. Translation: AAH37614.1.
CCDSCCDS49176.1. [Q9WVS4-1]
RefSeqNP_036103.1. NM_011973.2. [Q9WVS4-1]
UniGeneMm.140948.

3D structure databases

ProteinModelPortalQ9WVS4.
SMRQ9WVS4. Positions 1-332.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9WVS4. 1 interaction.

PTM databases

PhosphoSiteQ9WVS4.

Proteomic databases

PRIDEQ9WVS4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000070565; ENSMUSP00000068904; ENSMUSG00000056458. [Q9WVS4-1]
GeneID26448.
KEGGmmu:26448.
UCSCuc007pbv.1. mouse. [Q9WVS4-1]
uc007pbx.1. mouse. [Q9WVS4-2]

Organism-specific databases

CTD5891.
MGIMGI:1336881. Stk30.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00650000093283.
HOGENOMHOG000233024.
HOVERGENHBG106271.
InParanoidQ9WVS4.
KOK08830.
OMAILMLHEV.
PhylomeDBQ9WVS4.
TreeFamTF328769.

Gene expression databases

ArrayExpressQ9WVS4.
BgeeQ9WVS4.
CleanExMM_RAGE.
GenevestigatorQ9WVS4.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio304549.
PROQ9WVS4.
SOURCESearch...

Entry information

Entry nameMOK_MOUSE
AccessionPrimary (citable) accession number: Q9WVS4
Secondary accession number(s): Q8CFU4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: November 1, 1999
Last modified: July 9, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot