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Q9WVS4

- MOK_MOUSE

UniProt

Q9WVS4 - MOK_MOUSE

Protein

MAPK/MAK/MRK overlapping kinase

Gene

Mok

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Able to phosphorylate several exogenous substrates and to undergo autophosphorylation.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Phosphorylation appears to increase the enzymatic activity.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei33 – 331ATPPROSITE-ProRule annotation
    Active sitei128 – 1281Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 189ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    MAPK/MAK/MRK overlapping kinase (EC:2.7.11.22)
    Alternative name(s):
    MOK protein kinase
    Serine/threonine kinase 30
    Gene namesi
    Name:Mok
    Synonyms:Rage, Stk30
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 12

    Organism-specific databases

    MGIiMGI:1336881. Mok.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi159 – 1591T → A: Loss of activity; when associated with F-161. 1 Publication
    Mutagenesisi161 – 1611Y → F: Loss of activity; when associated with A-159. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 420420MAPK/MAK/MRK overlapping kinasePRO_0000086342Add
    BLAST

    Post-translational modificationi

    Autophosphorylated.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ9WVS4.

    PTM databases

    PhosphoSiteiQ9WVS4.

    Expressioni

    Tissue specificityi

    Highly expressed in testis, and less in kidney, brain and lung.

    Gene expression databases

    ArrayExpressiQ9WVS4.
    BgeeiQ9WVS4.
    CleanExiMM_RAGE.
    GenevestigatoriQ9WVS4.

    Interactioni

    Protein-protein interaction databases

    IntActiQ9WVS4. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9WVS4.
    SMRiQ9WVS4. Positions 1-332.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 285282Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00650000093283.
    HOGENOMiHOG000233024.
    HOVERGENiHBG106271.
    InParanoidiQ9WVS4.
    KOiK08830.
    OMAiILMLHEV.
    PhylomeDBiQ9WVS4.
    TreeFamiTF328769.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9WVS4-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKNYKAIGKI GEGTFSEVMK MQSLRDGNYY ACKQMKQHFE SIEQVNSLRE    50
    IQALRRLNPH PNILALHEVV FDRKSGSLAL ICELMDMNIY ELIRGRRHPL 100
    SEKKIMLYMY QLCKSLDHMH RNGIFHRDVK PENILVKQDV LKLGDFGSCR 150
    SVYSKQPYTE YISTRWYRAP ECLLTDGFYT YKMDLWSAGC VFYEIASLQP 200
    LFPGVNELDQ ISKIHDVIGT PCQKTLTKFK QSRAMSFDFP FKKGSGIPLL 250
    TANLSPQCLS LLHAMVAYDP DERIAAHQAL QHPYFQVQRA AETQTLAKHR 300
    RAFCPKFSMV PESSSHNWSF SQEGRKQKQS LRHEEGHARR QGPTSLMELP 350
    KLRLSGMTKL SSCSSPALRS VLGTGANGKV PVLRPLKCAA VNKKTDTQKD 400
    IKPHLKHYHL PTINRKGGEY 420
    Length:420
    Mass (Da):48,065
    Last modified:November 1, 1999 - v1
    Checksum:i1C637E1ECB33C998
    GO
    Isoform 2 (identifier: Q9WVS4-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-92: Missing.
         93-95: IRG → MKR
         232-289: Missing.
         328-420: KQSLRHEEGH...PTINRKGGEY → V

    Note: No experimental confirmation available.

    Show »
    Length:178
    Mass (Da):20,864
    Checksum:i95E8F3A2221673FB
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 9292Missing in isoform 2. 1 PublicationVSP_009147Add
    BLAST
    Alternative sequencei93 – 953IRG → MKR in isoform 2. 1 PublicationVSP_009148
    Alternative sequencei232 – 28958Missing in isoform 2. 1 PublicationVSP_009149Add
    BLAST
    Alternative sequencei328 – 42093KQSLR…KGGEY → V in isoform 2. 1 PublicationVSP_009150Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB022695 mRNA. Translation: BAA81689.1.
    BC037614 mRNA. Translation: AAH37614.1.
    CCDSiCCDS49176.1. [Q9WVS4-1]
    RefSeqiNP_036103.1. NM_011973.2. [Q9WVS4-1]
    UniGeneiMm.140948.

    Genome annotation databases

    EnsembliENSMUST00000070565; ENSMUSP00000068904; ENSMUSG00000056458. [Q9WVS4-1]
    GeneIDi26448.
    KEGGimmu:26448.
    UCSCiuc007pbv.1. mouse. [Q9WVS4-1]
    uc007pbx.1. mouse. [Q9WVS4-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB022695 mRNA. Translation: BAA81689.1 .
    BC037614 mRNA. Translation: AAH37614.1 .
    CCDSi CCDS49176.1. [Q9WVS4-1 ]
    RefSeqi NP_036103.1. NM_011973.2. [Q9WVS4-1 ]
    UniGenei Mm.140948.

    3D structure databases

    ProteinModelPortali Q9WVS4.
    SMRi Q9WVS4. Positions 1-332.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9WVS4. 1 interaction.

    PTM databases

    PhosphoSitei Q9WVS4.

    Proteomic databases

    PRIDEi Q9WVS4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000070565 ; ENSMUSP00000068904 ; ENSMUSG00000056458 . [Q9WVS4-1 ]
    GeneIDi 26448.
    KEGGi mmu:26448.
    UCSCi uc007pbv.1. mouse. [Q9WVS4-1 ]
    uc007pbx.1. mouse. [Q9WVS4-2 ]

    Organism-specific databases

    CTDi 5891.
    MGIi MGI:1336881. Mok.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00650000093283.
    HOGENOMi HOG000233024.
    HOVERGENi HBG106271.
    InParanoidi Q9WVS4.
    KOi K08830.
    OMAi ILMLHEV.
    PhylomeDBi Q9WVS4.
    TreeFami TF328769.

    Miscellaneous databases

    NextBioi 304549.
    PROi Q9WVS4.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9WVS4.
    Bgeei Q9WVS4.
    CleanExi MM_RAGE.
    Genevestigatori Q9WVS4.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of a novel member of the MAP kinase superfamily."
      Miyata Y., Akashi M., Nishida E.
      Genes Cells 4:299-309(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, AUTOPHOSPHORYLATION, MUTAGENESIS OF THR-159 AND TYR-161.
      Strain: C57BL/6.
      Tissue: Brain.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6.
      Tissue: Retina.

    Entry informationi

    Entry nameiMOK_MOUSE
    AccessioniPrimary (citable) accession number: Q9WVS4
    Secondary accession number(s): Q8CFU4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 16, 2004
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3