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Q9WVS0 (ICOS_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Inducible T-cell costimulator
Alternative name(s):
Activation-inducible lymphocyte immunomediatory molecule
CD28 and CTLA-4-like protein
Short name=CCLP
CD28-related protein 1
Short name=CRP-1
CD_antigen=CD278
Gene names
Name:Icos
Synonyms:Ailim
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length200 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up-regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes By similarity. Ref.1 Ref.4 Ref.7

Subunit structure

Homodimer; disulfide-linked. Ref.3

Subcellular location

Membrane; Single-pass type I membrane protein Potential.

Tissue specificity

Expressed on activated T-cells and resting memory T-cells. High expression seen in the thymic medulla and in the germinal centers and T-cell zones of lymph nodes and Peyer patches. Expressed at low levels in the spleen. Ref.1 Ref.2 Ref.3

Post-translational modification

N-glycosylated. Ref.3

Sequence similarities

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 200180Inducible T-cell costimulator
PRO_0000014807

Regions

Topological domain21 – 144124Extracellular Potential
Transmembrane145 – 16521Helical; Potential
Topological domain166 – 20035Cytoplasmic Potential
Domain30 – 133104Ig-like V-type

Amino acid modifications

Glycosylation231N-linked (GlcNAc...) Potential
Glycosylation891N-linked (GlcNAc...) Potential
Glycosylation1231N-linked (GlcNAc...) Potential
Disulfide bond42 ↔ 109 By similarity
Disulfide bond63 ↔ 83 By similarity

Experimental info

Sequence conflict71R → H Ref.2
Sequence conflict71R → H Ref.3
Sequence conflict71R → H Ref.5

Sequences

Sequence LengthMass (Da)Tools
Q9WVS0 [UniParc].

Last modified July 19, 2004. Version 2.
Checksum: 87D97F0DC44ADCA7

FASTA20022,709
        10         20         30         40         50         60 
MKPYFCRVFV FCFLIRLLTG EINGSADHRM FSFHNGGVQI SCKYPETVQQ LKMRLFRERE 

        70         80         90        100        110        120 
VLCELTKTKG SGNAVSIKNP MLCLYHLSNN SVSFFLNNPD SSQGSYYFCS LSIFDPPPFQ 

       130        140        150        160        170        180 
ERNLSGGYLH IYESQLCCQL KLWLPVGCAA FVVVLLFGCI LIIWFSKKKY GSSVHDPNSE 

       190        200 
YMFMAAVNTN KKSRLAGVTS 

« Hide

References

« Hide 'large scale' references
[1]"T-cell co-stimulation through B7RP-1 and ICOS."
Yoshinaga S.K., Whoriskey J.S., Khare S.D., Sarmiento U., Guo J., Horan T., Shih G., Zhang M., Coccia M.A., Kohno T., Tafuri-Bladt A., Brankow D., Campbell P., Chang D., Chiu L., Dai T., Duncan G., Elliott G.S. expand/collapse author list , Hui A., McCabe S.M., Scully S., Shahinian A., Shaklee C.L., Van G., Mak T.W., Senaldi G.
Nature 402:827-832(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE, FUNCTION, TISSUE SPECIFICITY.
Tissue: Intestinal epithelium.
[2]"Identification and characterization of rat AILIM/ICOS, a novel T-cell costimulatory molecule, related to the CD28/CTLA4 family."
Tezuka K., Tsuji T., Hirano D., Tamatani T., Sakamaki K., Kobayashi Y., Kamada M.
Biochem. Biophys. Res. Commun. 276:335-345(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE, TISSUE SPECIFICITY.
Tissue: Spleen.
[3]"Molecular cloning and characterization of murine ICOS and identification of B7h as ICOS ligand."
Mages H.W., Hutloff A., Heuck C., Buechner K., Himmelbauer H., Oliveri F., Kroczek R.A.
Eur. J. Immunol. 30:1040-1047(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], GLYCOSYLATION, SUBUNIT, TISSUE SPECIFICITY.
Strain: BALB/c.
Tissue: Spleen.
[4]"The inducible costimulatory (ICOS) molecule is critical for CD40-mediated antibody class switching."
McAdam A.J., Greenwald R.J., Levin M.A., Chernova T., Malenkovich N., Ling V., Freeman G.J., Sharpe A.H.
Nature 409:102-105(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE, FUNCTION.
Strain: 129/Ola.
[5]"CCLP, A novel molecule that regulates T cell activation."
Wu D., Giannoni M.A., Kiesecker C.L., Faas S.J., Mickle A.P., Matis L.A., Rother R.P.
Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: BALB/c.
Tissue: Spleen.
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Mammary gland.
[7]"ICOS is essential for effective T-helper-cell responses."
Tafuri A., Shahinian A., Bladt F., Yoshinaga S.K., Jordana M., Wakeham A., Boucher L.-M., Bouchard D., Chan V.S., Duncan G., Odermatt B., Ho A., Itie A., Horan T., Whoriskey J.S., Pawson T., Penninger J.M., Ohashi P.S., Mak T.W.
Nature 409:105-109(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF216748 mRNA. Translation: AAF45150.1.
AB023132 mRNA. Translation: BAA82126.1.
AJ250559 mRNA. Translation: CAB71153.1.
AF327185, AF327184 Genomic DNA. Translation: AAG48732.1.
AF257230 mRNA. Translation: AAF70099.1.
BC034852 mRNA. Translation: AAH34852.1.
CCDSCCDS14994.1.
RefSeqNP_059508.2. NM_017480.2.
XP_006496201.1. XM_006496138.1.
XP_006496202.1. XM_006496139.1.
XP_006536968.1. XM_006536905.1.
XP_006536969.1. XM_006536906.1.
UniGeneMm.42044.

3D structure databases

ProteinModelPortalQ9WVS0.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ9WVS0.

Proteomic databases

PRIDEQ9WVS0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000102827; ENSMUSP00000099891; ENSMUSG00000026009.
GeneID54167.
KEGGmmu:54167.
UCSCuc011wlz.2. mouse.

Organism-specific databases

CTD29851.
MGIMGI:1858745. Icos.

Phylogenomic databases

eggNOGNOG69972.
GeneTreeENSGT00390000000801.
HOGENOMHOG000112996.
HOVERGENHBG052079.
KOK06713.
OrthoDBEOG7JMGFN.
TreeFamTF335679.

Gene expression databases

ArrayExpressQ9WVS0.
BgeeQ9WVS0.
CleanExMM_ICOS.
GenevestigatorQ9WVS0.

Family and domain databases

ProtoNetSearch...

Other

NextBio311010.
PROQ9WVS0.
SOURCESearch...

Entry information

Entry nameICOS_MOUSE
AccessionPrimary (citable) accession number: Q9WVS0
Secondary accession number(s): Q9JL17
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 19, 2004
Last modified: July 9, 2014
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot