Q9WVS0 (ICOS_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inducible T-cell costimulator

Alternative name(s):
Activation-inducible lymphocyte immunomediatory molecule
CD28 and CTLA-4-like protein
Short name=CCLP
CD28-related protein 1
Short name=CRP-1
CD_antigen=CD278

Gene names

Name:	Icos
Synonyms:	Ailim

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	200 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. Essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. Does not up-regulate the production of interleukin-2, but superinduces the synthesis of interleukin-10. Prevents the apoptosis of pre-activated T-cells. Plays a critical role in CD40-mediated class switching of immunoglobin isotypes By similarity. Ref.1 Ref.4 Ref.7
Subunit structure	Homodimer; disulfide-linked. Ref.3
Subcellular location	Membrane; Single-pass type I membrane protein Potential.
Tissue specificity	Expressed on activated T-cells and resting memory T-cells. High expression seen in the thymic medulla and in the germinal centers and T-cell zones of lymph nodes and Peyer patches. Expressed at low levels in the spleen. Ref.1 Ref.2 Ref.3
Post-translational modification	N-glycosylated. Ref.3
Sequence similarities	Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Immunoglobulin domain Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	T cell costimulation Inferred from electronic annotation. Source: Compara T cell tolerance induction Inferred from electronic annotation. Source: Compara cell-cell adhesion Inferred from electronic annotation. Source: Compara
Cellular_component	external side of plasma membrane Inferred from direct assay Ref.3PubMed 16227984PubMed 19008373. Source: MGI integral to plasma membrane Inferred from direct assay Ref.3. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 200	180	Inducible T-cell costimulator		PRO_0000014807
Regions
	Topological domain	21 – 144	124	Extracellular Potential
	Transmembrane	145 – 165	21	Helical; Potential
	Topological domain	166 – 200	35	Cytoplasmic Potential
	Domain	30 – 133	104	Ig-like V-type
Amino acid modifications
	Glycosylation	23	1	N-linked (GlcNAc...) Potential
	Glycosylation	89	1	N-linked (GlcNAc...) Potential
	Glycosylation	123	1	N-linked (GlcNAc...) Potential
	Disulfide bond	42 ↔ 109		By similarity
	Disulfide bond	63 ↔ 83		By similarity
Experimental info
	Sequence conflict	7	1	R → H Ref.2
	Sequence conflict	7	1	R → H Ref.3
	Sequence conflict	7	1	R → H Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q9WVS0 [UniParc]. Last modified July 19, 2004. Version 2. Checksum: 87D97F0DC44ADCA7	FASTA	200	22,709
	10 20 30 40 50 60 MKPYFCRVFV FCFLIRLLTG EINGSADHRM FSFHNGGVQI SCKYPETVQQ LKMRLFRERE 70 80 90 100 110 120 VLCELTKTKG SGNAVSIKNP MLCLYHLSNN SVSFFLNNPD SSQGSYYFCS LSIFDPPPFQ 130 140 150 160 170 180 ERNLSGGYLH IYESQLCCQL KLWLPVGCAA FVVVLLFGCI LIIWFSKKKY GSSVHDPNSE 190 200 YMFMAAVNTN KKSRLAGVTS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"T-cell co-stimulation through B7RP-1 and ICOS." Yoshinaga S.K., Whoriskey J.S., Khare S.D., Sarmiento U., Guo J., Horan T., Shih G., Zhang M., Coccia M.A., Kohno T., Tafuri-Bladt A., Brankow D., Campbell P., Chang D., Chiu L., Dai T., Duncan G., Elliott G.S. , Hui A., McCabe S.M., Scully S., Shahinian A., Shaklee C.L., Van G., Mak T.W., Senaldi G. Nature 402:827-832(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, FUNCTION, TISSUE SPECIFICITY. Tissue: Intestinal epithelium.
[2]	"Identification and characterization of rat AILIM/ICOS, a novel T-cell costimulatory molecule, related to the CD28/CTLA4 family." Tezuka K., Tsuji T., Hirano D., Tamatani T., Sakamaki K., Kobayashi Y., Kamada M. Biochem. Biophys. Res. Commun. 276:335-345(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, TISSUE SPECIFICITY. Tissue: Spleen.
[3]	"Molecular cloning and characterization of murine ICOS and identification of B7h as ICOS ligand." Mages H.W., Hutloff A., Heuck C., Buechner K., Himmelbauer H., Oliveri F., Kroczek R.A. Eur. J. Immunol. 30:1040-1047(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], GLYCOSYLATION, SUBUNIT, TISSUE SPECIFICITY. Strain: BALB/c. Tissue: Spleen.
[4]	"The inducible costimulatory (ICOS) molecule is critical for CD40-mediated antibody class switching." McAdam A.J., Greenwald R.J., Levin M.A., Chernova T., Malenkovich N., Ling V., Freeman G.J., Sharpe A.H. Nature 409:102-105(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, FUNCTION. Strain: 129/Ola.
[5]	"CCLP, A novel molecule that regulates T cell activation." Wu D., Giannoni M.A., Kiesecker C.L., Faas S.J., Mickle A.P., Matis L.A., Rother R.P. Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: BALB/c. Tissue: Spleen.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Mammary gland.
[7]	"ICOS is essential for effective T-helper-cell responses." Tafuri A., Shahinian A., Bladt F., Yoshinaga S.K., Jordana M., Wakeham A., Boucher L.-M., Bouchard D., Chan V.S., Duncan G., Odermatt B., Ho A., Itie A., Horan T., Whoriskey J.S., Pawson T., Penninger J.M., Ohashi P.S., Mak T.W. Nature 409:105-109(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF216748 mRNA. Translation: AAF45150.1. AB023132 mRNA. Translation: BAA82126.1. AJ250559 mRNA. Translation: CAB71153.1. AF327185, AF327184 Genomic DNA. Translation: AAG48732.1. AF257230 mRNA. Translation: AAF70099.1. BC034852 mRNA. Translation: AAH34852.1.
IPI	IPI00311582.
RefSeq	NP_059508.2. NM_017480.2.
UniGene	Mm.42044.
3D structure databases
ProteinModelPortal	Q9WVS0.
ModBase	Search...
PTM databases
PhosphoSite	Q9WVS0.
Proteomic databases
PRIDE	Q9WVS0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000102827; ENSMUSP00000099891; ENSMUSG00000026009.
GeneID	54167.
KEGG	mmu:54167.
Organism-specific databases
CTD	29851.
MGI	MGI:1858745. Icos.
Phylogenomic databases
eggNOG	NOG69972.
GeneTree	ENSGT00390000000801.
HOGENOM	HOG000112996.
HOVERGEN	HBG052079.
KO	K06713.
OrthoDB	EOG4Q58QW.
Gene expression databases
ArrayExpress	Q9WVS0.
Bgee	Q9WVS0.
CleanEx	MM_ICOS.
Genevestigator	Q9WVS0.
Family and domain databases
PROSITE	PS50835. IG_LIKE. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	311010.
SOURCE	Search...

Entry information

Entry name

ICOS_MOUSE

Accession

Primary (citable) accession number: Q9WVS0
Secondary accession number(s): Q9JL17

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	July 19, 2004
Last modified:	May 1, 2013
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program