Q9WVR7 (PPM1F_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein phosphatase 1F
EC=3.1.3.16

Alternative name(s):
Ca(2+)/calmodulin-dependent protein kinase phosphatase
Short name=CaM-kinase phosphatase
Short name=CaMKPase
Partner of PIX 2

Gene names

Name:	Ppm1f
Synonyms:	Popx2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Dephosphorylates and concomitantly deactivates CaM-kinase II activated upon autophosphorylation, and CaM-kinases IV and I activated upon phosphorylation by CaM-kinase kinase. Promotes apoptosis.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Subunit structure	Associates with FEM1B By similarity.
Sequence similarities	Belongs to the PP2C family.

Ontologies

Keywords
Biological process	Apoptosis
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular response to drug Inferred from sequence or structural similarity. Source: UniProtKB histone dephosphorylation Inferred from direct assay PubMed 16844074. Source: RGD intrinsic apoptotic signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of peptidyl-serine phosphorylation Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of protein kinase activity by regulation of protein phosphorylation Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of transcription, DNA-dependent Inferred from sequence or structural similarity. Source: UniProtKB peptidyl-threonine dephosphorylation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of chemotaxis Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Inferred from direct assay PubMed 11559703. Source: UniProtKB positive regulation of epithelial cell migration Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of focal adhesion assembly Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of gene expression Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of growth Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of stress fiber assembly Inferred from electronic annotation. Source: Compara
Cellular_component	cytosol Inferred from direct assay Ref.1. Source: UniProtKB perinuclear region of cytoplasm Inferred from direct assay Ref.1. Source: UniProtKB protein complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	calmodulin-dependent protein phosphatase activity Inferred from sequence or structural similarity. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	Protein phosphatase 1F		PRO_0000057760
Regions
	Compositional bias	99 – 109	11	Poly-Glu
Sites
	Metal binding	194	1	Manganese 1 By similarity
	Metal binding	194	1	Manganese 2 By similarity
	Metal binding	195	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	356	1	Manganese 2 By similarity
	Metal binding	400	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9WVR7 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 67626542F32B2FD0	FASTA	450	49,165
	10 20 30 40 50 60 MASGAPQNSS QMACDGEIPG FLDTLLQDFP APLSLESPLP WKVPGTVLGQ EEVEAELTEL 70 80 90 100 110 120 AMGFLGSRNA PPAVAAAVTH EAISQLLQTD LSEFKRLPEQ EEEEEEEEER VLTTLLDAKG 130 140 150 160 170 180 LSRSFFNCLW EVCSQWQKRV PLTAQAPQRK WLVSIHAIRN TRRKMEDRHV SLPAFNHLFG 190 200 210 220 230 240 LSDSVHRAYF AVFDGHGGVD AARYASVHVH TNASHQPELL TDPAAALKEA FRHTDQMFLQ 250 260 270 280 290 300 KAKRERLQSG TTGVCALITG AALHVAWLGD SQVILVQQGQ VVKLMEPHKP ERQDEKSRIE 310 320 330 340 350 360 ALGGFVSLMD CWRVNGTLAV SRAIGDVFQK PYVSGEADAA SRELTGLEDY LLLACDGFFD 370 380 390 400 410 420 VVPHHEIPGL VHGHLLRQKG SGMHVAEELV AVARDRGSHD NITVMVVFLR DPLELLEGGG 430 440 450 QGAGGAQADV GSQDLSTGLS ELEINTSQRS

References

[1]	"Molecular cloning of Ca2+/Calmodulin-dependent protein kinase phosphatase." Kitani T., Ishida A., Okuno S., Takeuchi M., Kameshita I., Fujisawa H. J. Biochem. 125:1022-1028(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB023634 mRNA. Translation: BAA82477.1.
IPI	IPI00205297.
RefSeq	NP_786931.1. NM_175755.2.
UniGene	Rn.91922.
3D structure databases
ProteinModelPortal	Q9WVR7.
ModBase	Search...
PTM databases
PhosphoSite	Q9WVR7.
Proteomic databases
PaxDb	Q9WVR7.
PRIDE	Q9WVR7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000002530; ENSRNOP00000002530; ENSRNOG00000037909.
GeneID	287931.
KEGG	rno:287931.
Organism-specific databases
CTD	9647.
RGD	631363. Ppm1f.
Phylogenomic databases
eggNOG	COG0631.
GeneTree	ENSGT00690000101775.
HOGENOM	HOG000232094.
HOVERGEN	HBG053656.
InParanoid	Q9WVR7.
KO	K01090.
OMA	GDVFQKP.
OrthoDB	EOG4JM7PR.
Gene expression databases
ArrayExpress	Q9WVR7.
Genevestigator	Q9WVR7.
GermOnline	ENSRNOG00000037909. Rattus norvegicus.
Family and domain databases
Gene3D	3.60.40.10. 1 hit.
InterPro	IPR001932. PP2C-like. IPR000222. PP2C_Mn2_Asp60_BS. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	627246.

Entry information

Entry name

PPM1F_RAT

Accession

Primary (citable) accession number: Q9WVR7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
Last sequence update:	November 1, 1999
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program