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Q9WVR6 (LAT2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Large neutral amino acids transporter small subunit 2
Alternative name(s):
L-type amino acid transporter 2
Solute carrier family 7 member 8
Gene names
Name:Slc7a8
Synonyms:Lat2, Lat4
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length533 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Sodium-independent, high-affinity transport of small and large neutral amino acids such as alanine, serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney. Ref.1

Subunit structure

Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc. Ref.1

Subcellular location

Cytoplasm By similarity. Basolateral cell membrane; Multi-pass membrane protein. Note: Localized to the cytoplasm when expressed alone By similarity. When coexpressed with SLC3A2/4F2hc, is localized to the plasma membrane. Colocalized with SLC3A2/4F2hc at the basolateral membrane of kidney cortex proximal tubules and small intestine epithelia of the villi By similarity. Ref.2

Tissue specificity

Expression is seen in jejunum mucosa and the epithelial cells of the jejunum, ileum and colon, as well as in kidney, placenta, brain, testis and skeletal muscle. Expressed in retina, inner blood-retinal barrier of retina, retinal vascular endothelial cells. Also expressed in the intestinal epithelial cell line IEC-6 and in the retinal capillary endothelial cell line TR-iBRB2. Ref.1 Ref.2 Ref.3

Miscellaneous

Leucine transport activity is inhibited by 2-amino-bicyclo-(2,2,1)-heptane-2-carboxylate (BCH), glycine, L-isomers of the neutral amino acids and histidine.

Sequence similarities

Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family. [View classification]

Biophysicochemical properties

Kinetic parameters:

KM=119 µM for L-leucine Ref.1

KM=265 µM for glycine

KM=187 µM for L-alanine

KM=116 µM for L-serine

KM=68.6 µM for L-threonine

KM=109 µM for L-cysteine

KM=80.7 µM for L-asparginine

KM=151 µM for L-glutamine

KM=96.7 µM for L-isoleucine

KM=124 µM for L-valine

KM=204 µM for L-methionine

KM=45 µM for L-phenylalanine

KM=35.9 µM for L-tyrosine

KM=57.6 µM for L-tryptophan

KM=181 µM for L-histidine

pH dependence:

Optimum pH is 6.25.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 533533Large neutral amino acids transporter small subunit 2
PRO_0000054275

Regions

Transmembrane41 – 6121Helical; Potential
Transmembrane73 – 9321Helical; Potential
Transmembrane114 – 13421Helical; Potential
Transmembrane156 – 17621Helical; Potential
Transmembrane190 – 21021Helical; Potential
Transmembrane232 – 25221Helical; Potential
Transmembrane269 – 28921Helical; Potential
Transmembrane311 – 33121Helical; Potential
Transmembrane363 – 38321Helical; Potential
Transmembrane389 – 40921Helical; Potential
Transmembrane423 – 44321Helical; Potential
Transmembrane448 – 46821Helical; Potential

Sequences

Sequence LengthMass (Da)Tools
Q9WVR6 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 99479DB60DA69DF0

FASTA53358,190
        10         20         30         40         50         60 
MEKGTRQRNN TAKNHPDRGS DTSPEAEASS GGGGVALKKE IGLVSACGII VGNIIGSGIF 

        70         80         90        100        110        120 
VSPKGVLENA GSVGLALIVW IVTGVITAVG ALCYAELGVT IPKSGGDYSY VKDIFGGLAG 

       130        140        150        160        170        180 
FLRLWIAVLV IYPTNQAVIA LTFSNYVLQP LFPTCFPPES GLRLLAAICL LLLTWVNCSS 

       190        200        210        220        230        240 
VRWATRVQDI FTAGKLLALA LIIIMGVVQI CKGEFFWLEP KNAFENFQEP DIGLVALAFL 

       250        260        270        280        290        300 
QGSFAYGGWN FLNYVTEELV DPYKNLPRAI FISIPLVTFV YVFANIAYVT AMSPQELLAS 

       310        320        330        340        350        360 
NAVAVTFGEK LLGVMAWIMP ISVALSTFGG VNGSLFTSSR LFFAGAREGH LPSVLAMIHV 

       370        380        390        400        410        420 
KRCTPIPALL FTCLSTLLML VTSDMYTLIN YVGFINYLFY GVTVAGQIVL RWKKPDIPRP 

       430        440        450        460        470        480 
IKISLLFPII YLLFWAFLLI FSLWSEPVVC GIGLAIMLTG VPVYFLGVYW QHKPKCFNDF 

       490        500        510        520        530 
IESLTLVSQK MCVVVYPQEG DSGTEETIDD VEEQHKPIFQ PTPVKDPDSE EQP 

« Hide

References

[1]"Identification and functional characterization of a Na+-independent neutral amino acid transporter with broad substrate selectivity."
Segawa H., Fukasawa Y., Miyamoto K., Takeda E., Endou H., Kanai Y.
J. Biol. Chem. 274:19745-19751(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, TISSUE SPECIFICITY, INHIBITION.
Tissue: Small intestine.
[2]"Expression of LAT1 and LAT2 amino acid transporters in human and rat intestinal epithelial cells."
Fraga S., Pinho M.J., Soares-da-Silva P.
Amino Acids 29:229-233(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[3]"L-type amino acid transporter 1-mediated L-leucine transport at the inner blood-retinal barrier."
Tomi M., Mori M., Tachikawa M., Katayama K., Terasaki T., Hosoya K.
Invest. Ophthalmol. Vis. Sci. 46:2522-2530(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB024400 mRNA. Translation: BAA82517.1.
RefSeqNP_445894.1. NM_053442.1.
UniGeneRn.82734.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000019618.

Chemistry

BindingDBQ9WVR6.

Protein family/group databases

TCDB2.A.3.8.6. the amino acid-polyamine-organocation (apc) family.

Proteomic databases

PaxDbQ9WVR6.
PRIDEQ9WVR6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000019618; ENSRNOP00000019618; ENSRNOG00000014311.
GeneID84551.
KEGGrno:84551.
UCSCRGD:619904. rat.

Organism-specific databases

CTD23428.
RGD619904. Slc7a8.

Phylogenomic databases

eggNOGCOG0531.
GeneTreeENSGT00730000110280.
HOGENOMHOG000098892.
HOVERGENHBG000476.
InParanoidQ9WVR6.
KOK13781.
OMALIVWIIT.
OrthoDBEOG73BVCR.
PhylomeDBQ9WVR6.
TreeFamTF313355.

Gene expression databases

GenevestigatorQ9WVR6.

Family and domain databases

InterProIPR002293. AA/rel_permease1.
IPR004760. L_AA_transporter.
[Graphical view]
PANTHERPTHR11785. PTHR11785. 1 hit.
PfamPF13520. AA_permease_2. 1 hit.
[Graphical view]
PIRSFPIRSF006060. AA_transporter. 1 hit.
TIGRFAMsTIGR00911. 2A0308. 1 hit.
ProtoNetSearch...

Other

NextBio617101.
PROQ9WVR6.

Entry information

Entry nameLAT2_RAT
AccessionPrimary (citable) accession number: Q9WVR6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: November 1, 1999
Last modified: April 16, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families