Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9WVR6

- LAT2_RAT

UniProt

Q9WVR6 - LAT2_RAT

Protein

Large neutral amino acids transporter small subunit 2

Gene

Slc7a8

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Sodium-independent, high-affinity transport of small and large neutral amino acids such as alanine, serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney.1 Publication

    Kineticsi

    1. KM=119 µM for L-leucine1 Publication
    2. KM=265 µM for glycine1 Publication
    3. KM=187 µM for L-alanine1 Publication
    4. KM=116 µM for L-serine1 Publication
    5. KM=68.6 µM for L-threonine1 Publication
    6. KM=109 µM for L-cysteine1 Publication
    7. KM=80.7 µM for L-asparginine1 Publication
    8. KM=151 µM for L-glutamine1 Publication
    9. KM=96.7 µM for L-isoleucine1 Publication
    10. KM=124 µM for L-valine1 Publication
    11. KM=204 µM for L-methionine1 Publication
    12. KM=45 µM for L-phenylalanine1 Publication
    13. KM=35.9 µM for L-tyrosine1 Publication
    14. KM=57.6 µM for L-tryptophan1 Publication
    15. KM=181 µM for L-histidine1 Publication

    pH dependencei

    Optimum pH is 6.25.1 Publication

    GO - Molecular functioni

    1. amino acid transmembrane transporter activity Source: UniProtKB
    2. L-amino acid transmembrane transporter activity Source: Ensembl
    3. organic cation transmembrane transporter activity Source: Ensembl
    4. peptide antigen binding Source: UniProtKB
    5. toxin transporter activity Source: Ensembl

    GO - Biological processi

    1. neutral amino acid transport Source: UniProtKB

    Keywords - Biological processi

    Amino-acid transport, Transport

    Protein family/group databases

    TCDBi2.A.3.8.6. the amino acid-polyamine-organocation (apc) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Large neutral amino acids transporter small subunit 2
    Alternative name(s):
    L-type amino acid transporter 2
    Solute carrier family 7 member 8
    Gene namesi
    Name:Slc7a8
    Synonyms:Lat2, Lat4
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 15

    Organism-specific databases

    RGDi619904. Slc7a8.

    Subcellular locationi

    Cytoplasm By similarity. Basolateral cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
    Note: Localized to the cytoplasm when expressed alone By similarity. When coexpressed with SLC3A2/4F2hc, is localized to the plasma membrane. Colocalized with SLC3A2/4F2hc at the basolateral membrane of kidney cortex proximal tubules and small intestine epithelia of the villi By similarity.By similarity

    GO - Cellular componenti

    1. basolateral plasma membrane Source: UniProtKB-SubCell
    2. cytoplasm Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 533533Large neutral amino acids transporter small subunit 2PRO_0000054275Add
    BLAST

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiQ9WVR6.
    PRIDEiQ9WVR6.

    Expressioni

    Tissue specificityi

    Expression is seen in jejunum mucosa and the epithelial cells of the jejunum, ileum and colon, as well as in kidney, placenta, brain, testis and skeletal muscle. Expressed in retina, inner blood-retinal barrier of retina, retinal vascular endothelial cells. Also expressed in the intestinal epithelial cell line IEC-6 and in the retinal capillary endothelial cell line TR-iBRB2.3 Publications

    Gene expression databases

    GenevestigatoriQ9WVR6.

    Interactioni

    Subunit structurei

    Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc.1 Publication

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000019618.

    Structurei

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei41 – 6121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei73 – 9321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei114 – 13421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei156 – 17621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei190 – 21021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei232 – 25221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei269 – 28921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei311 – 33121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei363 – 38321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei389 – 40921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei423 – 44321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei448 – 46821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0531.
    GeneTreeiENSGT00730000110280.
    HOGENOMiHOG000098892.
    HOVERGENiHBG000476.
    InParanoidiQ9WVR6.
    KOiK13781.
    OMAiAFEPFQD.
    OrthoDBiEOG73BVCR.
    PhylomeDBiQ9WVR6.
    TreeFamiTF313355.

    Family and domain databases

    InterProiIPR002293. AA/rel_permease1.
    IPR004760. L_AA_transporter.
    [Graphical view]
    PANTHERiPTHR11785. PTHR11785. 1 hit.
    PfamiPF13520. AA_permease_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006060. AA_transporter. 1 hit.
    TIGRFAMsiTIGR00911. 2A0308. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9WVR6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEKGTRQRNN TAKNHPDRGS DTSPEAEASS GGGGVALKKE IGLVSACGII    50
    VGNIIGSGIF VSPKGVLENA GSVGLALIVW IVTGVITAVG ALCYAELGVT 100
    IPKSGGDYSY VKDIFGGLAG FLRLWIAVLV IYPTNQAVIA LTFSNYVLQP 150
    LFPTCFPPES GLRLLAAICL LLLTWVNCSS VRWATRVQDI FTAGKLLALA 200
    LIIIMGVVQI CKGEFFWLEP KNAFENFQEP DIGLVALAFL QGSFAYGGWN 250
    FLNYVTEELV DPYKNLPRAI FISIPLVTFV YVFANIAYVT AMSPQELLAS 300
    NAVAVTFGEK LLGVMAWIMP ISVALSTFGG VNGSLFTSSR LFFAGAREGH 350
    LPSVLAMIHV KRCTPIPALL FTCLSTLLML VTSDMYTLIN YVGFINYLFY 400
    GVTVAGQIVL RWKKPDIPRP IKISLLFPII YLLFWAFLLI FSLWSEPVVC 450
    GIGLAIMLTG VPVYFLGVYW QHKPKCFNDF IESLTLVSQK MCVVVYPQEG 500
    DSGTEETIDD VEEQHKPIFQ PTPVKDPDSE EQP 533
    Length:533
    Mass (Da):58,190
    Last modified:November 1, 1999 - v1
    Checksum:i99479DB60DA69DF0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB024400 mRNA. Translation: BAA82517.1.
    RefSeqiNP_445894.1. NM_053442.1.
    UniGeneiRn.82734.

    Genome annotation databases

    EnsembliENSRNOT00000019618; ENSRNOP00000019618; ENSRNOG00000014311.
    GeneIDi84551.
    KEGGirno:84551.
    UCSCiRGD:619904. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB024400 mRNA. Translation: BAA82517.1 .
    RefSeqi NP_445894.1. NM_053442.1.
    UniGenei Rn.82734.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000019618.

    Chemistry

    BindingDBi Q9WVR6.

    Protein family/group databases

    TCDBi 2.A.3.8.6. the amino acid-polyamine-organocation (apc) family.

    Proteomic databases

    PaxDbi Q9WVR6.
    PRIDEi Q9WVR6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000019618 ; ENSRNOP00000019618 ; ENSRNOG00000014311 .
    GeneIDi 84551.
    KEGGi rno:84551.
    UCSCi RGD:619904. rat.

    Organism-specific databases

    CTDi 23428.
    RGDi 619904. Slc7a8.

    Phylogenomic databases

    eggNOGi COG0531.
    GeneTreei ENSGT00730000110280.
    HOGENOMi HOG000098892.
    HOVERGENi HBG000476.
    InParanoidi Q9WVR6.
    KOi K13781.
    OMAi AFEPFQD.
    OrthoDBi EOG73BVCR.
    PhylomeDBi Q9WVR6.
    TreeFami TF313355.

    Miscellaneous databases

    NextBioi 617101.
    PROi Q9WVR6.

    Gene expression databases

    Genevestigatori Q9WVR6.

    Family and domain databases

    InterProi IPR002293. AA/rel_permease1.
    IPR004760. L_AA_transporter.
    [Graphical view ]
    PANTHERi PTHR11785. PTHR11785. 1 hit.
    Pfami PF13520. AA_permease_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006060. AA_transporter. 1 hit.
    TIGRFAMsi TIGR00911. 2A0308. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Identification and functional characterization of a Na+-independent neutral amino acid transporter with broad substrate selectivity."
      Segawa H., Fukasawa Y., Miyamoto K., Takeda E., Endou H., Kanai Y.
      J. Biol. Chem. 274:19745-19751(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, TISSUE SPECIFICITY, INHIBITION.
      Tissue: Small intestine.
    2. "Expression of LAT1 and LAT2 amino acid transporters in human and rat intestinal epithelial cells."
      Fraga S., Pinho M.J., Soares-da-Silva P.
      Amino Acids 29:229-233(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    3. "L-type amino acid transporter 1-mediated L-leucine transport at the inner blood-retinal barrier."
      Tomi M., Mori M., Tachikawa M., Katayama K., Terasaki T., Hosoya K.
      Invest. Ophthalmol. Vis. Sci. 46:2522-2530(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiLAT2_RAT
    AccessioniPrimary (citable) accession number: Q9WVR6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 24, 2001
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Leucine transport activity is inhibited by 2-amino-bicyclo-(2,2,1)-heptane-2-carboxylate (BCH), glycine, L-isomers of the neutral amino acids and histidine.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3