Large neutral amino acids transporter small subunit 2

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Q9WVR6 (LAT2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 88. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Large neutral amino acids transporter small subunit 2

Alternative name(s):
L-type amino acid transporter 2
Solute carrier family 7 member 8

Gene names

Name:	Slc7a8
Synonyms:	Lat2, Lat4

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	533 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Sodium-independent, high-affinity transport of small and large neutral amino acids such as alanine, serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney. Ref.1
Subunit structure	Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc. Ref.1
Subcellular location	Cytoplasm By similarity. Basolateral cell membrane; Multi-pass membrane protein. Note: Localized to the cytoplasm when expressed alone By similarity. When coexpressed with SLC3A2/4F2hc, is localized to the plasma membrane. Colocalized with SLC3A2/4F2hc at the basolateral membrane of kidney cortex proximal tubules and small intestine epithelia of the villi By similarity. Ref.2
Tissue specificity	Expression is seen in jejunum mucosa and the epithelial cells of the jejunum, ileum and colon, as well as in kidney, placenta, brain, testis and skeletal muscle. Expressed in retina, inner blood-retinal barrier of retina, retinal vascular endothelial cells. Also expressed in the intestinal epithelial cell line IEC-6 and in the retinal capillary endothelial cell line TR-iBRB2. Ref.1 Ref.2 Ref.3
Miscellaneous	Leucine transport activity is inhibited by 2-amino-bicyclo-(2,2,1)-heptane-2-carboxylate (BCH), glycine, L-isomers of the neutral amino acids and histine.
Sequence similarities	Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family. [View classification]
Biophysicochemical properties	Kinetic parameters: K_M=119 µM for L-leucine Ref.1 K_M=265 µM for glycine K_M=187 µM for L-alanine K_M=116 µM for L-serine K_M=68.6 µM for L-threonine K_M=109 µM for L-cysteine K_M=80.7 µM for L-asparginine K_M=151 µM for L-glutamine K_M=96.7 µM for L-isoleucine K_M=124 µM for L-valine K_M=204 µM for L-methionine K_M=45 µM for L-phenylalanine K_M=35.9 µM for L-tyrosine K_M=57.6 µM for L-tryptophan K_M=181 µM for L-histidine pH dependence: Optimum pH is 6.25.

Ontologies

Keywords
Biological process	Amino-acid transport Transport
Cellular component	Cell membrane Cytoplasm Membrane
Domain	Transmembrane Transmembrane helix
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	neutral amino acid transport Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	basolateral plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	L-amino acid transmembrane transporter activity Inferred from electronic annotation. Source: Compara amino acid transmembrane transporter activity Inferred from sequence or structural similarity. Source: UniProtKB organic cation transmembrane transporter activity Inferred from electronic annotation. Source: Compara peptide antigen binding Inferred from sequence or structural similarity. Source: UniProtKB toxin transporter activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 533

533

Large neutral amino acids transporter small subunit 2

PRO_0000054275

Regions

Transmembrane

41 – 61

Helical; Potential

Transmembrane

73 – 93

Helical; Potential

Transmembrane

114 – 134

Helical; Potential

Transmembrane

156 – 176

Helical; Potential

Transmembrane

190 – 210

Helical; Potential

Transmembrane

232 – 252

Helical; Potential

Transmembrane

269 – 289

Helical; Potential

Transmembrane

311 – 331

Helical; Potential

Transmembrane

363 – 383

Helical; Potential

Transmembrane

389 – 409

Helical; Potential

Transmembrane

423 – 443

Helical; Potential

Transmembrane

448 – 468

Helical; Potential

Sequences

Sequence

Length

Mass (Da)

Tools

Q9WVR6 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 99479DB60DA69DF0
Show »

FASTA

533

58,190

        10         20         30         40         50         60 
MEKGTRQRNN TAKNHPDRGS DTSPEAEASS GGGGVALKKE IGLVSACGII VGNIIGSGIF 

        70         80         90        100        110        120 
VSPKGVLENA GSVGLALIVW IVTGVITAVG ALCYAELGVT IPKSGGDYSY VKDIFGGLAG 

       130        140        150        160        170        180 
FLRLWIAVLV IYPTNQAVIA LTFSNYVLQP LFPTCFPPES GLRLLAAICL LLLTWVNCSS 

       190        200        210        220        230        240 
VRWATRVQDI FTAGKLLALA LIIIMGVVQI CKGEFFWLEP KNAFENFQEP DIGLVALAFL 

       250        260        270        280        290        300 
QGSFAYGGWN FLNYVTEELV DPYKNLPRAI FISIPLVTFV YVFANIAYVT AMSPQELLAS 

       310        320        330        340        350        360 
NAVAVTFGEK LLGVMAWIMP ISVALSTFGG VNGSLFTSSR LFFAGAREGH LPSVLAMIHV 

       370        380        390        400        410        420 
KRCTPIPALL FTCLSTLLML VTSDMYTLIN YVGFINYLFY GVTVAGQIVL RWKKPDIPRP 

       430        440        450        460        470        480 
IKISLLFPII YLLFWAFLLI FSLWSEPVVC GIGLAIMLTG VPVYFLGVYW QHKPKCFNDF 

       490        500        510        520        530 
IESLTLVSQK MCVVVYPQEG DSGTEETIDD VEEQHKPIFQ PTPVKDPDSE EQP

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References

[1]	"Identification and functional characterization of a Na+-independent neutral amino acid transporter with broad substrate selectivity." Segawa H., Fukasawa Y., Miyamoto K., Takeda E., Endou H., Kanai Y. J. Biol. Chem. 274:19745-19751(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, TISSUE SPECIFICITY, INHIBITION. Tissue: Small intestine.
[2]	"Expression of LAT1 and LAT2 amino acid transporters in human and rat intestinal epithelial cells." Fraga S., Pinho M.J., Soares-da-Silva P. Amino Acids 29:229-233(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[3]	"L-type amino acid transporter 1-mediated L-leucine transport at the inner blood-retinal barrier." Tomi M., Mori M., Tachikawa M., Katayama K., Terasaki T., Hosoya K. Invest. Ophthalmol. Vis. Sci. 46:2522-2530(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB024400 mRNA. Translation: BAA82517.1.
IPI	IPI00205295.
RefSeq	NP_445894.1. NM_053442.1.
UniGene	Rn.82734.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000019618.
Protein family/group databases
TCDB	2.A.3.8.6. amino acid-polyamine-organocation (APC) family.
Proteomic databases
PaxDb	Q9WVR6.
PRIDE	Q9WVR6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000019618; ENSRNOP00000019618; ENSRNOG00000014311.
GeneID	84551.
KEGG	rno:84551.
UCSC	RGD:619904. rat.
Organism-specific databases
CTD	23428.
RGD	619904. Slc7a8.
Phylogenomic databases
eggNOG	COG0531.
GeneTree	ENSGT00690000101731.
HOGENOM	HOG000098892.
HOVERGEN	HBG000476.
InParanoid	Q9WVR6.
KO	K13781.
OMA	FVDKMTY.
OrthoDB	EOG4V437M.
Gene expression databases
Genevestigator	Q9WVR6.
GermOnline	ENSRNOG00000014311. Rattus norvegicus.
Family and domain databases
InterPro	IPR002293. AA/rel_permease1. IPR004760. L_AA_transporter. [Graphical view]
PANTHER	PTHR11785. PTHR11785. 1 hit.
PIRSF	PIRSF006060. AA_transporter. 1 hit.
TIGRFAMs	TIGR00911. 2A0308. 1 hit.
ProtoNet	Search...
Other
BindingDB	Q9WVR6.
ChEMBL	CHEMBL4585.
NextBio	617101.

Entry information

Entry name

LAT2_RAT

Accession

Primary (citable) accession number: Q9WVR6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	November 1, 1999
Last modified:	April 3, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents