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Q9WVR6

- LAT2_RAT

UniProt

Q9WVR6 - LAT2_RAT

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Protein

Large neutral amino acids transporter small subunit 2

Gene
Slc7a8, Lat2, Lat4
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Sodium-independent, high-affinity transport of small and large neutral amino acids such as alanine, serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney.1 Publication

Kineticsi

  1. KM=119 µM for L-leucine1 Publication
  2. KM=265 µM for glycine
  3. KM=187 µM for L-alanine
  4. KM=116 µM for L-serine
  5. KM=68.6 µM for L-threonine
  6. KM=109 µM for L-cysteine
  7. KM=80.7 µM for L-asparginine
  8. KM=151 µM for L-glutamine
  9. KM=96.7 µM for L-isoleucine
  10. KM=124 µM for L-valine
  11. KM=204 µM for L-methionine
  12. KM=45 µM for L-phenylalanine
  13. KM=35.9 µM for L-tyrosine
  14. KM=57.6 µM for L-tryptophan
  15. KM=181 µM for L-histidine

pH dependencei

Optimum pH is 6.25.

GO - Molecular functioni

  1. amino acid transmembrane transporter activity Source: UniProtKB
  2. L-amino acid transmembrane transporter activity Source: Ensembl
  3. organic cation transmembrane transporter activity Source: Ensembl
  4. peptide antigen binding Source: UniProtKB
  5. toxin transporter activity Source: Ensembl

GO - Biological processi

  1. neutral amino acid transport Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Protein family/group databases

TCDBi2.A.3.8.6. the amino acid-polyamine-organocation (apc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Large neutral amino acids transporter small subunit 2
Alternative name(s):
L-type amino acid transporter 2
Solute carrier family 7 member 8
Gene namesi
Name:Slc7a8
Synonyms:Lat2, Lat4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 15

Organism-specific databases

RGDi619904. Slc7a8.

Subcellular locationi

Cytoplasm By similarity. Basolateral cell membrane; Multi-pass membrane protein
Note: Localized to the cytoplasm when expressed alone By similarity. When coexpressed with SLC3A2/4F2hc, is localized to the plasma membrane. Colocalized with SLC3A2/4F2hc at the basolateral membrane of kidney cortex proximal tubules and small intestine epithelia of the villi By similarity.1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei41 – 6121Helical; Reviewed predictionAdd
BLAST
Transmembranei73 – 9321Helical; Reviewed predictionAdd
BLAST
Transmembranei114 – 13421Helical; Reviewed predictionAdd
BLAST
Transmembranei156 – 17621Helical; Reviewed predictionAdd
BLAST
Transmembranei190 – 21021Helical; Reviewed predictionAdd
BLAST
Transmembranei232 – 25221Helical; Reviewed predictionAdd
BLAST
Transmembranei269 – 28921Helical; Reviewed predictionAdd
BLAST
Transmembranei311 – 33121Helical; Reviewed predictionAdd
BLAST
Transmembranei363 – 38321Helical; Reviewed predictionAdd
BLAST
Transmembranei389 – 40921Helical; Reviewed predictionAdd
BLAST
Transmembranei423 – 44321Helical; Reviewed predictionAdd
BLAST
Transmembranei448 – 46821Helical; Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. basolateral plasma membrane Source: UniProtKB-SubCell
  2. cytoplasm Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 533533Large neutral amino acids transporter small subunit 2PRO_0000054275Add
BLAST

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ9WVR6.
PRIDEiQ9WVR6.

Expressioni

Tissue specificityi

Expression is seen in jejunum mucosa and the epithelial cells of the jejunum, ileum and colon, as well as in kidney, placenta, brain, testis and skeletal muscle. Expressed in retina, inner blood-retinal barrier of retina, retinal vascular endothelial cells. Also expressed in the intestinal epithelial cell line IEC-6 and in the retinal capillary endothelial cell line TR-iBRB2.3 Publications

Gene expression databases

GenevestigatoriQ9WVR6.

Interactioni

Subunit structurei

Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc.1 Publication

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000019618.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0531.
GeneTreeiENSGT00730000110280.
HOGENOMiHOG000098892.
HOVERGENiHBG000476.
InParanoidiQ9WVR6.
KOiK13781.
OMAiAFEPFQD.
OrthoDBiEOG73BVCR.
PhylomeDBiQ9WVR6.
TreeFamiTF313355.

Family and domain databases

InterProiIPR002293. AA/rel_permease1.
IPR004760. L_AA_transporter.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF13520. AA_permease_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.
TIGRFAMsiTIGR00911. 2A0308. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9WVR6-1 [UniParc]FASTAAdd to Basket

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MEKGTRQRNN TAKNHPDRGS DTSPEAEASS GGGGVALKKE IGLVSACGII    50
VGNIIGSGIF VSPKGVLENA GSVGLALIVW IVTGVITAVG ALCYAELGVT 100
IPKSGGDYSY VKDIFGGLAG FLRLWIAVLV IYPTNQAVIA LTFSNYVLQP 150
LFPTCFPPES GLRLLAAICL LLLTWVNCSS VRWATRVQDI FTAGKLLALA 200
LIIIMGVVQI CKGEFFWLEP KNAFENFQEP DIGLVALAFL QGSFAYGGWN 250
FLNYVTEELV DPYKNLPRAI FISIPLVTFV YVFANIAYVT AMSPQELLAS 300
NAVAVTFGEK LLGVMAWIMP ISVALSTFGG VNGSLFTSSR LFFAGAREGH 350
LPSVLAMIHV KRCTPIPALL FTCLSTLLML VTSDMYTLIN YVGFINYLFY 400
GVTVAGQIVL RWKKPDIPRP IKISLLFPII YLLFWAFLLI FSLWSEPVVC 450
GIGLAIMLTG VPVYFLGVYW QHKPKCFNDF IESLTLVSQK MCVVVYPQEG 500
DSGTEETIDD VEEQHKPIFQ PTPVKDPDSE EQP 533
Length:533
Mass (Da):58,190
Last modified:November 1, 1999 - v1
Checksum:i99479DB60DA69DF0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB024400 mRNA. Translation: BAA82517.1.
RefSeqiNP_445894.1. NM_053442.1.
UniGeneiRn.82734.

Genome annotation databases

EnsembliENSRNOT00000019618; ENSRNOP00000019618; ENSRNOG00000014311.
GeneIDi84551.
KEGGirno:84551.
UCSCiRGD:619904. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB024400 mRNA. Translation: BAA82517.1 .
RefSeqi NP_445894.1. NM_053442.1.
UniGenei Rn.82734.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000019618.

Chemistry

BindingDBi Q9WVR6.

Protein family/group databases

TCDBi 2.A.3.8.6. the amino acid-polyamine-organocation (apc) family.

Proteomic databases

PaxDbi Q9WVR6.
PRIDEi Q9WVR6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000019618 ; ENSRNOP00000019618 ; ENSRNOG00000014311 .
GeneIDi 84551.
KEGGi rno:84551.
UCSCi RGD:619904. rat.

Organism-specific databases

CTDi 23428.
RGDi 619904. Slc7a8.

Phylogenomic databases

eggNOGi COG0531.
GeneTreei ENSGT00730000110280.
HOGENOMi HOG000098892.
HOVERGENi HBG000476.
InParanoidi Q9WVR6.
KOi K13781.
OMAi AFEPFQD.
OrthoDBi EOG73BVCR.
PhylomeDBi Q9WVR6.
TreeFami TF313355.

Miscellaneous databases

NextBioi 617101.
PROi Q9WVR6.

Gene expression databases

Genevestigatori Q9WVR6.

Family and domain databases

InterProi IPR002293. AA/rel_permease1.
IPR004760. L_AA_transporter.
[Graphical view ]
PANTHERi PTHR11785. PTHR11785. 1 hit.
Pfami PF13520. AA_permease_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006060. AA_transporter. 1 hit.
TIGRFAMsi TIGR00911. 2A0308. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Identification and functional characterization of a Na+-independent neutral amino acid transporter with broad substrate selectivity."
    Segawa H., Fukasawa Y., Miyamoto K., Takeda E., Endou H., Kanai Y.
    J. Biol. Chem. 274:19745-19751(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, TISSUE SPECIFICITY, INHIBITION.
    Tissue: Small intestine.
  2. "Expression of LAT1 and LAT2 amino acid transporters in human and rat intestinal epithelial cells."
    Fraga S., Pinho M.J., Soares-da-Silva P.
    Amino Acids 29:229-233(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  3. "L-type amino acid transporter 1-mediated L-leucine transport at the inner blood-retinal barrier."
    Tomi M., Mori M., Tachikawa M., Katayama K., Terasaki T., Hosoya K.
    Invest. Ophthalmol. Vis. Sci. 46:2522-2530(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiLAT2_RAT
AccessioniPrimary (citable) accession number: Q9WVR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: November 1, 1999
Last modified: May 14, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Leucine transport activity is inhibited by 2-amino-bicyclo-(2,2,1)-heptane-2-carboxylate (BCH), glycine, L-isomers of the neutral amino acids and histidine.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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