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Protein

Large neutral amino acids transporter small subunit 2

Gene

Slc7a8

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Sodium-independent, high-affinity transport of small and large neutral amino acids such as alanine, serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney.1 Publication

Miscellaneous

Leucine transport activity is inhibited by 2-amino-bicyclo-(2,2,1)-heptane-2-carboxylate (BCH), glycine, L-isomers of the neutral amino acids and histidine.

Kineticsi

  1. KM=119 µM for L-leucine1 Publication
  2. KM=265 µM for glycine1 Publication
  3. KM=187 µM for L-alanine1 Publication
  4. KM=116 µM for L-serine1 Publication
  5. KM=68.6 µM for L-threonine1 Publication
  6. KM=109 µM for L-cysteine1 Publication
  7. KM=80.7 µM for L-asparagine1 Publication
  8. KM=151 µM for L-glutamine1 Publication
  9. KM=96.7 µM for L-isoleucine1 Publication
  10. KM=124 µM for L-valine1 Publication
  11. KM=204 µM for L-methionine1 Publication
  12. KM=45 µM for L-phenylalanine1 Publication
  13. KM=35.9 µM for L-tyrosine1 Publication
  14. KM=57.6 µM for L-tryptophan1 Publication
  15. KM=181 µM for L-histidine1 Publication

    pH dependencei

    Optimum pH is 6.25.1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Biological processAmino-acid transport, Transport

    Enzyme and pathway databases

    ReactomeiR-RNO-210991 Basigin interactions
    R-RNO-352230 Amino acid transport across the plasma membrane
    SABIO-RKQ9WVR6

    Protein family/group databases

    TCDBi2.A.3.8.6 the amino acid-polyamine-organocation (apc) family

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Large neutral amino acids transporter small subunit 2
    Alternative name(s):
    L-type amino acid transporter 2
    Solute carrier family 7 member 8
    Gene namesi
    Name:Slc7a8
    Synonyms:Lat2, Lat4
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
    Proteomesi
    • UP000002494 Componenti: Chromosome 15

    Organism-specific databases

    RGDi619904 Slc7a8

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei41 – 61HelicalSequence analysisAdd BLAST21
    Transmembranei73 – 93HelicalSequence analysisAdd BLAST21
    Transmembranei114 – 134HelicalSequence analysisAdd BLAST21
    Transmembranei156 – 176HelicalSequence analysisAdd BLAST21
    Transmembranei190 – 210HelicalSequence analysisAdd BLAST21
    Transmembranei232 – 252HelicalSequence analysisAdd BLAST21
    Transmembranei269 – 289HelicalSequence analysisAdd BLAST21
    Transmembranei311 – 331HelicalSequence analysisAdd BLAST21
    Transmembranei363 – 383HelicalSequence analysisAdd BLAST21
    Transmembranei389 – 409HelicalSequence analysisAdd BLAST21
    Transmembranei423 – 443HelicalSequence analysisAdd BLAST21
    Transmembranei448 – 468HelicalSequence analysisAdd BLAST21

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000542751 – 533Large neutral amino acids transporter small subunit 2Add BLAST533

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei20PhosphoserineBy similarity1
    Modified residuei23PhosphoserineCombined sources1
    Modified residuei29PhosphoserineCombined sources1
    Modified residuei30PhosphoserineCombined sources1
    Modified residuei529PhosphoserineCombined sources1

    Keywords - PTMi

    Disulfide bond, Phosphoprotein

    Proteomic databases

    PaxDbiQ9WVR6
    PRIDEiQ9WVR6

    PTM databases

    iPTMnetiQ9WVR6
    PhosphoSitePlusiQ9WVR6

    Expressioni

    Tissue specificityi

    Expression is seen in jejunum mucosa and the epithelial cells of the jejunum, ileum and colon, as well as in kidney, placenta, brain, testis and skeletal muscle. Expressed in retina, inner blood-retinal barrier of retina, retinal vascular endothelial cells. Also expressed in the intestinal epithelial cell line IEC-6 and in the retinal capillary endothelial cell line TR-iBRB2.3 Publications

    Gene expression databases

    BgeeiENSRNOG00000014311
    GenevisibleiQ9WVR6 RN

    Interactioni

    Subunit structurei

    Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc.1 Publication

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000019618

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1287 Eukaryota
    COG0531 LUCA
    GeneTreeiENSGT00760000119037
    HOGENOMiHOG000098892
    HOVERGENiHBG000476
    InParanoidiQ9WVR6
    KOiK13781
    OMAiANAFEPF
    OrthoDBiEOG091G07EM
    PhylomeDBiQ9WVR6
    TreeFamiTF313355

    Family and domain databases

    InterProiView protein in InterPro
    IPR002293 AA/rel_permease1
    IPR004760 L_AA_transporter
    PfamiView protein in Pfam
    PF13520 AA_permease_2, 1 hit
    PIRSFiPIRSF006060 AA_transporter, 1 hit
    TIGRFAMsiTIGR00911 2A0308, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q9WVR6-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MEKGTRQRNN TAKNHPDRGS DTSPEAEASS GGGGVALKKE IGLVSACGII
    60 70 80 90 100
    VGNIIGSGIF VSPKGVLENA GSVGLALIVW IVTGVITAVG ALCYAELGVT
    110 120 130 140 150
    IPKSGGDYSY VKDIFGGLAG FLRLWIAVLV IYPTNQAVIA LTFSNYVLQP
    160 170 180 190 200
    LFPTCFPPES GLRLLAAICL LLLTWVNCSS VRWATRVQDI FTAGKLLALA
    210 220 230 240 250
    LIIIMGVVQI CKGEFFWLEP KNAFENFQEP DIGLVALAFL QGSFAYGGWN
    260 270 280 290 300
    FLNYVTEELV DPYKNLPRAI FISIPLVTFV YVFANIAYVT AMSPQELLAS
    310 320 330 340 350
    NAVAVTFGEK LLGVMAWIMP ISVALSTFGG VNGSLFTSSR LFFAGAREGH
    360 370 380 390 400
    LPSVLAMIHV KRCTPIPALL FTCLSTLLML VTSDMYTLIN YVGFINYLFY
    410 420 430 440 450
    GVTVAGQIVL RWKKPDIPRP IKISLLFPII YLLFWAFLLI FSLWSEPVVC
    460 470 480 490 500
    GIGLAIMLTG VPVYFLGVYW QHKPKCFNDF IESLTLVSQK MCVVVYPQEG
    510 520 530
    DSGTEETIDD VEEQHKPIFQ PTPVKDPDSE EQP
    Length:533
    Mass (Da):58,190
    Last modified:November 1, 1999 - v1
    Checksum:i99479DB60DA69DF0
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB024400 mRNA Translation: BAA82517.1
    RefSeqiNP_445894.1, NM_053442.1
    UniGeneiRn.82734

    Genome annotation databases

    EnsembliENSRNOT00000019618; ENSRNOP00000019618; ENSRNOG00000014311
    ENSRNOT00000092160; ENSRNOP00000072188; ENSRNOG00000014311
    GeneIDi84551
    KEGGirno:84551
    UCSCiRGD:619904 rat

    Similar proteinsi

    Entry informationi

    Entry nameiLAT2_RAT
    AccessioniPrimary (citable) accession number: Q9WVR6
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2001
    Last sequence update: November 1, 1999
    Last modified: May 23, 2018
    This is version 127 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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