Q9WVR1 (INP5E_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
72 kDa inositol polyphosphate 5-phosphatase
EC=3.1.3.36

Alternative name(s):
5-phosphatase that induces arborization
Short name=Pharbin
Phosphatidylinositol polyphosphate 5-phosphatase type IV
Phosphatidylinositol-4,5-bisphosphate 5-phosphatase

Gene names

Name:

Inpp5e

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	647 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Converts phosphatidylinositol-3,4,5-triphosphate (PtdIns 3,4,5-P3) to PtdIns-P2. Can convert lipid substrates, but also water soluble inositol phosphates. Ref.1
Catalytic activity	1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1-phosphatidyl-1D-myo-inositol 4-phosphate + phosphate.
Subcellular location	Cytoplasm › cytoskeleton › cilium axoneme By similarity. Golgi apparatus › Golgi stack membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Note: Peripheral membrane protein associated with Golgi stacks By similarity. Ref.1
Post-translational modification	Phosphorylated upon DNA damage, probably by ATM or ATR By similarity.
Sequence similarities	Belongs to the inositol-1,4,5-trisphosphate 5-phosphatase type IV family.
Caution	The N-terminus was extended using the genomic sequence, in analogy to ortholog sequences.
Sequence caution	The sequence BAA82150.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Lipid metabolism
Cellular component	Cell projection Cilium Cytoplasm Cytoskeleton Golgi apparatus Membrane
Domain	Repeat
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	lipid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of neuron projection development Inferred from mutant phenotype Ref.1. Source: RGD
Cellular component	Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cilium axoneme Inferred from sequence or structural similarity. Source: UniProtKB cytoskeleton Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay Ref.1. Source: RGD
Molecular function	inositol-polyphosphate 5-phosphatase activity Inferred from mutant phenotype Ref.1. Source: RGD phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 647	647	72 kDa inositol polyphosphate 5-phosphatase		PRO_0000209749
Regions
	Repeat	59 – 62	4	1
	Repeat	76 – 79	4	2
	Repeat	147 – 150	4	3
	Repeat	239 – 242	4	4
	Region	59 – 242	184	4 X 4 AA repeats of P-X-X-P
	Compositional bias	96 – 101	6	Poly-Arg
Amino acid modifications
	Modified residue	103	1	Phosphoserine By similarity
	Modified residue	244	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9WVR1 [UniParc]. Last modified March 29, 2004. Version 2. Checksum: DAF4ED31BDD3F972	FASTA	647	72,238
	10 20 30 40 50 60 MPSKSACLRH TEAPGQLEGR MLQGQLSNPE KKLIPTSASL PAADSQSSQT NSMPPLSMPA 70 80 90 100 110 120 KPSNQNLQAK ANLITPQPPI RPKLERTLSL DDKGWRRRRF RGSQEDLTVQ NGASPCRGSL 130 140 150 160 170 180 QDSVAQSPAY SRPLPCLSTS LQEIPKPRRP QAAREGAHPC GVTVFLEMIS TSWTSCTERV 190 200 210 220 230 240 PQVGHSRLAS LRATHTPPAM DLNIASSSLR TANKVDPEHT DYKLRMQNRL VRAHSNLGPS 250 260 270 280 290 300 RPRSPLAGDD HSIHSARSSF SLLAPIRTKD IRSRSYLEGS LLASGALLGA DELARYFPDR 310 320 330 340 350 360 NMALFVATWN MQGQKELPAS LDEFLLPTEA DYTQDLYVIG VQEGCSDRRE WETRLQETLG 370 380 390 400 410 420 PQYVLLSSAA HGVLYMSLFI RRDLIWFCSE VEYSTVTTRI VSQIKTKGAL GVSFTFFGTS 430 440 450 460 470 480 FLFITSHFTS GDGKVAERLL DYNRTIQALA LPRNVPDTNP YRSSAGDVTT RFDEVFWFGD 490 500 510 520 530 540 FNFRLSGGRV AVEAFLKQDP EVDVLALLQH DQLTREMKKG SIFKGFEEAE IHFLPSYKFD 550 560 570 580 590 600 IGKDTYDSTS KQRTPSYTDR VLYKSRHKGD ICPMKYSSCP GIKTSDHRPV YGLFRVKVRP 610 620 630 640 GRDNIPLAAG KFDRELYLIG IKRRISKEIQ RQEALKSQSS SAVCTVS

References

[1]

"Pharbin, a novel inositol polyphosphate 5-phosphatase, induces dendritic appearances in fibroblasts." Asano T., Mochizuki Y., Matsumoto K., Takenawa T., Endo T. Biochem. Biophys. Res. Commun. 261:188-195(1999) [PubMed: 10405344] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION. Tissue: Brain.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB026288 mRNA. Translation: BAA82150.1. Different initiation.
IPI	IPI00205288.
UniGene	Rn.199218.
3D structure databases
ProteinModelPortal	Q9WVR1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9WVR1.
PTM databases
PhosphoSite	Q9WVR1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
RGD	620478. Inpp5e.
Phylogenomic databases
eggNOG	roNOG13252.
GeneTree	ENSGT00600000084387.
HOVERGEN	HBG052132.
Gene expression databases
ArrayExpress	Q9WVR1.
Genevestigator	Q9WVR1.
GermOnline	ENSRNOG00000019039. Rattus norvegicus.
Family and domain databases
InterPro	IPR005135. Endo/exonuclease/phosphatase. IPR000300. IPPc. [Graphical view]
Pfam	PF03372. Exo_endo_phos. 1 hit. [Graphical view]
SMART	SM00128. IPPc. 1 hit. [Graphical view]
SUPFAM	SSF56219. Exo_endo_phos. 1 hit.
ProtoNet	Search...

Entry information

Entry name

INP5E_RAT

Accession

Primary (citable) accession number: Q9WVR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2004
Last sequence update:	March 29, 2004
Last modified:	September 21, 2011
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program