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Q9WVP6

- PAPOB_MOUSE

UniProt

Q9WVP6 - PAPOB_MOUSE

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Protein

Poly(A) polymerase beta

Gene

Papolb

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Binds 2 magnesium ions. Also active with manganese (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei109 – 1091ATPBy similarity
Metal bindingi113 – 1131Magnesium 1; catalyticBy similarity
Metal bindingi113 – 1131Magnesium 2; catalyticBy similarity
Metal bindingi115 – 1151Magnesium 1; catalyticBy similarity
Metal bindingi115 – 1151Magnesium 2; catalyticBy similarity
Sitei153 – 1531Interaction with RNABy similarity
Sitei158 – 1581Interaction with RNABy similarity
Metal bindingi167 – 1671Magnesium 2; catalyticBy similarity
Binding sitei167 – 1671ATPBy similarity
Binding sitei228 – 2281ATPBy similarity
Binding sitei237 – 2371ATPBy similarity
Sitei328 – 3281Interaction with RNABy similarity
Sitei399 – 3991Interaction with RNABy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi100 – 1023ATPBy similarity
Nucleotide bindingi113 – 1153ATPBy similarity
Nucleotide bindingi246 – 2472ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. polynucleotide adenylyltransferase activity Source: MGI
  4. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. mRNA polyadenylation Source: RefGenome
  2. mRNA splicing, via spliceosome Source: RefGenome
  3. RNA polyadenylation Source: MGI
  4. termination of RNA polymerase II transcription Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) polymerase beta (EC:2.7.7.19)
Short name:
PAP-beta
Alternative name(s):
Polynucleotide adenylyltransferase beta
Testis-specific poly(A) polymerase
Gene namesi
Name:Papolb
Synonyms:Papt, Tpap
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:1932115. Papolb.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. endoplasmic reticulum Source: MGI
  3. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 641641Poly(A) polymerase betaPRO_0000051623Add
BLAST

Proteomic databases

PaxDbiQ9WVP6.
PRIDEiQ9WVP6.

PTM databases

PhosphoSiteiQ9WVP6.

Expressioni

Tissue specificityi

Testis specific.1 Publication

Developmental stagei

Expressed at low levels in 2-week-old mice. Abundantly expressed in 4-week-old and 6-week-old animals.1 Publication

Gene expression databases

BgeeiQ9WVP6.
CleanExiMM_PAPOLB.
ExpressionAtlasiQ9WVP6. baseline.
GenevestigatoriQ9WVP6.

Interactioni

Subunit structurei

Interacts with GSG1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Gsg1Q8R1W28EBI-7842113,EBI-7842142

Protein-protein interaction databases

BioGridi208033. 1 interaction.
IntActiQ9WVP6. 3 interactions.
MINTiMINT-6167909.
STRINGi10090.ENSMUSP00000100595.

Structurei

3D structure databases

ProteinModelPortaliQ9WVP6.
SMRiQ9WVP6. Positions 19-498.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the poly(A) polymerase family.Curated

Phylogenomic databases

eggNOGiCOG5186.
HOVERGENiHBG053502.
InParanoidiQ9WVP6.
KOiK14376.
OMAiMSDGMAR.

Family and domain databases

Gene3Di3.30.70.590. 1 hit.
InterProiIPR002934. Nucleotidyltransferase.
IPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
[Graphical view]
PfamiPF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 1 hit.
[Graphical view]
PIRSFiPIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMiSSF55003. SSF55003. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9WVP6 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPFAVTTQGA QQPAPAPKQF GISSPISLAA PKDTDRELTQ KLIETLQPFG
60 70 80 90 100
VFEEEEELQR RILILQKLNN LVKEWIREIS ESRNLPQAVI ENVGGKIFTF
110 120 130 140 150
GSYRLGVHTK GADIDALCVA PRHVDRNDFF TSFYDKLKLQ EEVKDLRAVE
160 170 180 190 200
EAFVPVIKLC FDGIEIDILF ARLALQTIPE DLDLRDDSLL KNLDIRCIRS
210 220 230 240 250
LNGCRVTDEI LHLVPNIDSF RLTLRAIKLW AKCHNIYSNI LGFLGGVSWA
260 270 280 290 300
MLVARTCQLY PNAIASTLVR KFFLVFSEWE WPNPVLLKEP EERNLNLPVW
310 320 330 340 350
DPRVNPSDRY HLMPIITPAY PQQNSTYNVS VSTRMVMIEE FKQGLAITHE
360 370 380 390 400
ILLNKAEWSK LFEAPSFFQK YKHYIVLLAS APTEKQHLEW VGLVESKIRI
410 420 430 440 450
LVGSLEKNEF ITLAHVNPQS FPAPKETADK EEFRTMWVIG LVLKKPENSE
460 470 480 490 500
ILSIDLTYDI QSFTDTVYRQ AINSKMFEMD MKIAAMHLRR KELHQLLPNH
510 520 530 540 550
VLQKKETHLT ESVRLTAVTD SSLLLSIDSE NSMTAPSPTG TMKTGPLTGN
560 570 580 590 600
PQGRNSPALA VMAASVTNIQ FPDVSLQHVN PIESSGIALS ESIPQIPSQP
610 620 630 640
TISPPPKPTM TRVVSSTHLV NHPSRPSGNT ATNIPNPILG V
Length:641
Mass (Da):72,198
Last modified:September 26, 2001 - v2
Checksum:iE10EE49B140A1086
GO

Sequence cautioni

The sequence BAA83086.1 differs from that shown. Reason: Erroneous initiation.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti201 – 2011L → F in AAD43624. (PubMed:11150526)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF039957 mRNA. Translation: AAD43624.1.
AB030729 mRNA. Translation: BAA83086.1. Different initiation.
RefSeqiNP_064327.1. NM_019943.2.
UniGeneiMm.441372.

Genome annotation databases

GeneIDi56522.
KEGGimmu:56522.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF039957 mRNA. Translation: AAD43624.1 .
AB030729 mRNA. Translation: BAA83086.1 . Different initiation.
RefSeqi NP_064327.1. NM_019943.2.
UniGenei Mm.441372.

3D structure databases

ProteinModelPortali Q9WVP6.
SMRi Q9WVP6. Positions 19-498.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 208033. 1 interaction.
IntActi Q9WVP6. 3 interactions.
MINTi MINT-6167909.
STRINGi 10090.ENSMUSP00000100595.

PTM databases

PhosphoSitei Q9WVP6.

Proteomic databases

PaxDbi Q9WVP6.
PRIDEi Q9WVP6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 56522.
KEGGi mmu:56522.

Organism-specific databases

CTDi 56903.
MGIi MGI:1932115. Papolb.

Phylogenomic databases

eggNOGi COG5186.
HOVERGENi HBG053502.
InParanoidi Q9WVP6.
KOi K14376.
OMAi MSDGMAR.

Miscellaneous databases

NextBioi 312850.
PROi Q9WVP6.
SOURCEi Search...

Gene expression databases

Bgeei Q9WVP6.
CleanExi MM_PAPOLB.
ExpressionAtlasi Q9WVP6. baseline.
Genevestigatori Q9WVP6.

Family and domain databases

Gene3Di 3.30.70.590. 1 hit.
InterProi IPR002934. Nucleotidyltransferase.
IPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
[Graphical view ]
Pfami PF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 1 hit.
[Graphical view ]
PIRSFi PIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMi SSF55003. SSF55003. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "An intronless gene encoding a poly(A) polymerase is specifically expressed in testis."
    Lee Y.J., Lee Y., Chung J.H.
    FEBS Lett. 487:287-292(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: BALB/c.
    Tissue: Testis.
  2. "Identification of a novel isoform of poly(A) polymerase, TPAP, specifically present in the cytoplasm of spermatogenic cells."
    Kashiwabara S., Zhuang T., Yamagata K., Noguchi J., Fukamizu A., Baba T.
    Dev. Biol. 228:106-115(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
    Strain: ddY.
    Tissue: Testis.
  3. "Germ cell-specific gene 1 targets testis-specific poly(A) polymerase to the endoplasmic reticulum through protein-protein interactions."
    Choi H.-S., Lee S.-H., Kim H., Lee Y.
    FEBS Lett. 582:1203-1209(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GSG1.

Entry informationi

Entry nameiPAPOB_MOUSE
AccessioniPrimary (citable) accession number: Q9WVP6
Secondary accession number(s): Q9R1R3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: September 26, 2001
Last modified: October 29, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3