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Q9WVP6 (PAPOB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Poly(A) polymerase beta

Short name=PAP-beta
EC=2.7.7.19
Alternative name(s):
Polynucleotide adenylyltransferase beta
Testis-specific poly(A) polymerase
Gene names
Name:Papolb
Synonyms:Papt, Tpap
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length641 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactor

Binds 2 magnesium ions. Also active with manganese By similarity.

Subunit structure

Interacts with GSG1. Ref.3

Subcellular location

Cytoplasm. Nucleus Ref.1 Ref.2.

Tissue specificity

Testis specific. Ref.1

Developmental stage

Expressed at low levels in 2-week-old mice. Abundantly expressed in 4-week-old and 6-week-old animals. Ref.1

Sequence similarities

Belongs to the poly(A) polymerase family.

Sequence caution

The sequence BAA83086.1 differs from that shown. Reason: Erroneous initiation.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Gsg1Q8R1W28EBI-7842113,EBI-7842142

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 641641Poly(A) polymerase beta
PRO_0000051623

Regions

Nucleotide binding100 – 1023ATP By similarity
Nucleotide binding113 – 1153ATP By similarity
Nucleotide binding246 – 2472ATP By similarity

Sites

Metal binding1131Magnesium 1; catalytic By similarity
Metal binding1131Magnesium 2; catalytic By similarity
Metal binding1151Magnesium 1; catalytic By similarity
Metal binding1151Magnesium 2; catalytic By similarity
Metal binding1671Magnesium 2; catalytic By similarity
Binding site1091ATP By similarity
Binding site1671ATP By similarity
Binding site2281ATP By similarity
Binding site2371ATP By similarity
Site1531Interaction with RNA By similarity
Site1581Interaction with RNA By similarity
Site3281Interaction with RNA By similarity
Site3991Interaction with RNA By similarity

Experimental info

Sequence conflict2011L → F in AAD43624. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9WVP6 [UniParc].

Last modified September 26, 2001. Version 2.
Checksum: E10EE49B140A1086

FASTA64172,198
        10         20         30         40         50         60 
MPFAVTTQGA QQPAPAPKQF GISSPISLAA PKDTDRELTQ KLIETLQPFG VFEEEEELQR 

        70         80         90        100        110        120 
RILILQKLNN LVKEWIREIS ESRNLPQAVI ENVGGKIFTF GSYRLGVHTK GADIDALCVA 

       130        140        150        160        170        180 
PRHVDRNDFF TSFYDKLKLQ EEVKDLRAVE EAFVPVIKLC FDGIEIDILF ARLALQTIPE 

       190        200        210        220        230        240 
DLDLRDDSLL KNLDIRCIRS LNGCRVTDEI LHLVPNIDSF RLTLRAIKLW AKCHNIYSNI 

       250        260        270        280        290        300 
LGFLGGVSWA MLVARTCQLY PNAIASTLVR KFFLVFSEWE WPNPVLLKEP EERNLNLPVW 

       310        320        330        340        350        360 
DPRVNPSDRY HLMPIITPAY PQQNSTYNVS VSTRMVMIEE FKQGLAITHE ILLNKAEWSK 

       370        380        390        400        410        420 
LFEAPSFFQK YKHYIVLLAS APTEKQHLEW VGLVESKIRI LVGSLEKNEF ITLAHVNPQS 

       430        440        450        460        470        480 
FPAPKETADK EEFRTMWVIG LVLKKPENSE ILSIDLTYDI QSFTDTVYRQ AINSKMFEMD 

       490        500        510        520        530        540 
MKIAAMHLRR KELHQLLPNH VLQKKETHLT ESVRLTAVTD SSLLLSIDSE NSMTAPSPTG 

       550        560        570        580        590        600 
TMKTGPLTGN PQGRNSPALA VMAASVTNIQ FPDVSLQHVN PIESSGIALS ESIPQIPSQP 

       610        620        630        640 
TISPPPKPTM TRVVSSTHLV NHPSRPSGNT ATNIPNPILG V 

« Hide

References

[1]"An intronless gene encoding a poly(A) polymerase is specifically expressed in testis."
Lee Y.J., Lee Y., Chung J.H.
FEBS Lett. 487:287-292(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Strain: BALB/c.
Tissue: Testis.
[2]"Identification of a novel isoform of poly(A) polymerase, TPAP, specifically present in the cytoplasm of spermatogenic cells."
Kashiwabara S., Zhuang T., Yamagata K., Noguchi J., Fukamizu A., Baba T.
Dev. Biol. 228:106-115(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
Strain: ddY.
Tissue: Testis.
[3]"Germ cell-specific gene 1 targets testis-specific poly(A) polymerase to the endoplasmic reticulum through protein-protein interactions."
Choi H.-S., Lee S.-H., Kim H., Lee Y.
FEBS Lett. 582:1203-1209(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GSG1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF039957 mRNA. Translation: AAD43624.1.
AB030729 mRNA. Translation: BAA83086.1. Different initiation.
RefSeqNP_064327.1. NM_019943.2.
UniGeneMm.441372.

3D structure databases

ProteinModelPortalQ9WVP6.
SMRQ9WVP6. Positions 19-498.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid208033. 1 interaction.
IntActQ9WVP6. 3 interactions.
MINTMINT-6167909.
STRING10090.ENSMUSP00000100595.

PTM databases

PhosphoSiteQ9WVP6.

Proteomic databases

PaxDbQ9WVP6.
PRIDEQ9WVP6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID56522.
KEGGmmu:56522.

Organism-specific databases

CTD56903.
MGIMGI:1932115. Papolb.

Phylogenomic databases

eggNOGCOG5186.
HOVERGENHBG053502.
InParanoidQ9WVP6.
KOK14376.
OMAMSDGMAR.

Gene expression databases

BgeeQ9WVP6.
CleanExMM_PAPOLB.
GenevestigatorQ9WVP6.

Family and domain databases

Gene3D3.30.70.590. 1 hit.
InterProIPR002934. Nucleotidyltransferase.
IPR011068. NuclTrfase_I_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
[Graphical view]
PfamPF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 1 hit.
[Graphical view]
PIRSFPIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMSSF55003. SSF55003. 1 hit.
ProtoNetSearch...

Other

NextBio312850.
PROQ9WVP6.
SOURCESearch...

Entry information

Entry namePAPOB_MOUSE
AccessionPrimary (citable) accession number: Q9WVP6
Secondary accession number(s): Q9R1R3
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: September 26, 2001
Last modified: April 16, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot