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Protein

Poly(A) polymerase beta

Gene

Papolb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium ions. Also active with manganese.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei109ATPBy similarity1
Metal bindingi113Magnesium 1; catalyticBy similarity1
Metal bindingi113Magnesium 2; catalyticBy similarity1
Metal bindingi115Magnesium 1; catalyticBy similarity1
Metal bindingi115Magnesium 2; catalyticBy similarity1
Metal bindingi167Magnesium 2; catalyticBy similarity1
Binding sitei167ATPBy similarity1
Binding sitei228ATPBy similarity1
Binding sitei237ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi100 – 102ATPBy similarity3
Nucleotide bindingi113 – 115ATPBy similarity3
Nucleotide bindingi246 – 247ATPBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding, Transferase
Biological processmRNA processing
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.7.19. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) polymerase beta (EC:2.7.7.19)
Short name:
PAP-beta
Alternative name(s):
Polynucleotide adenylyltransferase beta
Testis-specific poly(A) polymerase
Gene namesi
Name:Papolb
Synonyms:Papt, Tpap
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1932115. Papolb.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000516231 – 641Poly(A) polymerase betaAdd BLAST641

Proteomic databases

MaxQBiQ9WVP6.
PaxDbiQ9WVP6.
PRIDEiQ9WVP6.

PTM databases

iPTMnetiQ9WVP6.
PhosphoSitePlusiQ9WVP6.

Expressioni

Tissue specificityi

Testis specific.1 Publication

Developmental stagei

Expressed at low levels in 2-week-old mice. Abundantly expressed in 4-week-old and 6-week-old animals.1 Publication

Gene expression databases

CleanExiMM_PAPOLB.

Interactioni

Subunit structurei

Interacts with GSG1.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei153Interaction with RNABy similarity1
Sitei158Interaction with RNABy similarity1
Sitei328Interaction with RNABy similarity1
Sitei399Interaction with RNABy similarity1

Binary interactionsi

WithEntry#Exp.IntActNotes
Gsg1Q8R1W28EBI-7842113,EBI-7842142

Protein-protein interaction databases

BioGridi208033. 1 interactor.
IntActiQ9WVP6. 3 interactors.
MINTiMINT-6167909.
STRINGi10090.ENSMUSP00000100595.

Structurei

3D structure databases

ProteinModelPortaliQ9WVP6.
SMRiQ9WVP6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the poly(A) polymerase family.Curated

Phylogenomic databases

eggNOGiKOG2245. Eukaryota.
COG5186. LUCA.
HOVERGENiHBG053502.
InParanoidiQ9WVP6.
KOiK14376.
OMAiMHLRRKE.

Family and domain databases

InterProiView protein in InterPro
IPR011068. NuclTrfase_I-like_C.
IPR007012. PolA_pol_cen_dom.
IPR007010. PolA_pol_RNA-bd_dom.
IPR014492. PolyA_polymerase.
IPR002934. Polymerase_NTP_transf_dom.
PANTHERiPTHR10682. PTHR10682. 1 hit.
PfamiView protein in Pfam
PF01909. NTP_transf_2. 1 hit.
PF04928. PAP_central. 1 hit.
PF04926. PAP_RNA-bind. 1 hit.
PIRSFiPIRSF018425. PolyA_polymerase. 1 hit.
SUPFAMiSSF55003. SSF55003. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9WVP6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFAVTTQGA QQPAPAPKQF GISSPISLAA PKDTDRELTQ KLIETLQPFG
60 70 80 90 100
VFEEEEELQR RILILQKLNN LVKEWIREIS ESRNLPQAVI ENVGGKIFTF
110 120 130 140 150
GSYRLGVHTK GADIDALCVA PRHVDRNDFF TSFYDKLKLQ EEVKDLRAVE
160 170 180 190 200
EAFVPVIKLC FDGIEIDILF ARLALQTIPE DLDLRDDSLL KNLDIRCIRS
210 220 230 240 250
LNGCRVTDEI LHLVPNIDSF RLTLRAIKLW AKCHNIYSNI LGFLGGVSWA
260 270 280 290 300
MLVARTCQLY PNAIASTLVR KFFLVFSEWE WPNPVLLKEP EERNLNLPVW
310 320 330 340 350
DPRVNPSDRY HLMPIITPAY PQQNSTYNVS VSTRMVMIEE FKQGLAITHE
360 370 380 390 400
ILLNKAEWSK LFEAPSFFQK YKHYIVLLAS APTEKQHLEW VGLVESKIRI
410 420 430 440 450
LVGSLEKNEF ITLAHVNPQS FPAPKETADK EEFRTMWVIG LVLKKPENSE
460 470 480 490 500
ILSIDLTYDI QSFTDTVYRQ AINSKMFEMD MKIAAMHLRR KELHQLLPNH
510 520 530 540 550
VLQKKETHLT ESVRLTAVTD SSLLLSIDSE NSMTAPSPTG TMKTGPLTGN
560 570 580 590 600
PQGRNSPALA VMAASVTNIQ FPDVSLQHVN PIESSGIALS ESIPQIPSQP
610 620 630 640
TISPPPKPTM TRVVSSTHLV NHPSRPSGNT ATNIPNPILG V
Length:641
Mass (Da):72,198
Last modified:September 26, 2001 - v2
Checksum:iE10EE49B140A1086
GO

Sequence cautioni

The sequence BAA83086 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti201L → F in AAD43624 (PubMed:11150526).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039957 mRNA. Translation: AAD43624.1.
AB030729 mRNA. Translation: BAA83086.1. Different initiation.
RefSeqiNP_064327.1. NM_019943.2.
UniGeneiMm.441372.

Genome annotation databases

GeneIDi56522.
KEGGimmu:56522.

Similar proteinsi

Entry informationi

Entry nameiPAPOB_MOUSE
AccessioniPrimary (citable) accession number: Q9WVP6
Secondary accession number(s): Q9R1R3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: September 26, 2001
Last modified: November 22, 2017
This is version 133 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families