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Q9WVP6

- PAPOB_MOUSE

UniProt

Q9WVP6 - PAPOB_MOUSE

Protein

Poly(A) polymerase beta

Gene

Papolb

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 2 (26 Sep 2001)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + RNA(n) = diphosphate + RNA(n+1).

    Cofactori

    Binds 2 magnesium ions. Also active with manganese By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei109 – 1091ATPBy similarity
    Metal bindingi113 – 1131Magnesium 1; catalyticBy similarity
    Metal bindingi113 – 1131Magnesium 2; catalyticBy similarity
    Metal bindingi115 – 1151Magnesium 1; catalyticBy similarity
    Metal bindingi115 – 1151Magnesium 2; catalyticBy similarity
    Sitei153 – 1531Interaction with RNABy similarity
    Sitei158 – 1581Interaction with RNABy similarity
    Metal bindingi167 – 1671Magnesium 2; catalyticBy similarity
    Binding sitei167 – 1671ATPBy similarity
    Binding sitei228 – 2281ATPBy similarity
    Binding sitei237 – 2371ATPBy similarity
    Sitei328 – 3281Interaction with RNABy similarity
    Sitei399 – 3991Interaction with RNABy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi100 – 1023ATPBy similarity
    Nucleotide bindingi113 – 1153ATPBy similarity
    Nucleotide bindingi246 – 2472ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. polynucleotide adenylyltransferase activity Source: MGI
    4. protein binding Source: IntAct
    5. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. mRNA polyadenylation Source: RefGenome
    2. mRNA splicing, via spliceosome Source: RefGenome
    3. RNA polyadenylation Source: MGI
    4. termination of RNA polymerase II transcription Source: RefGenome

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    mRNA processing

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding, RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Poly(A) polymerase beta (EC:2.7.7.19)
    Short name:
    PAP-beta
    Alternative name(s):
    Polynucleotide adenylyltransferase beta
    Testis-specific poly(A) polymerase
    Gene namesi
    Name:Papolb
    Synonyms:Papt, Tpap
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:1932115. Papolb.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. endoplasmic reticulum Source: MGI
    3. nucleus Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 641641Poly(A) polymerase betaPRO_0000051623Add
    BLAST

    Proteomic databases

    PaxDbiQ9WVP6.
    PRIDEiQ9WVP6.

    PTM databases

    PhosphoSiteiQ9WVP6.

    Expressioni

    Tissue specificityi

    Testis specific.1 Publication

    Developmental stagei

    Expressed at low levels in 2-week-old mice. Abundantly expressed in 4-week-old and 6-week-old animals.1 Publication

    Gene expression databases

    BgeeiQ9WVP6.
    CleanExiMM_PAPOLB.
    GenevestigatoriQ9WVP6.

    Interactioni

    Subunit structurei

    Interacts with GSG1.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Gsg1Q8R1W28EBI-7842113,EBI-7842142

    Protein-protein interaction databases

    BioGridi208033. 1 interaction.
    IntActiQ9WVP6. 3 interactions.
    MINTiMINT-6167909.
    STRINGi10090.ENSMUSP00000100595.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9WVP6.
    SMRiQ9WVP6. Positions 19-498.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the poly(A) polymerase family.Curated

    Phylogenomic databases

    eggNOGiCOG5186.
    HOVERGENiHBG053502.
    InParanoidiQ9WVP6.
    KOiK14376.
    OMAiMSDGMAR.

    Family and domain databases

    Gene3Di3.30.70.590. 1 hit.
    InterProiIPR002934. Nucleotidyltransferase.
    IPR011068. NuclTrfase_I_C.
    IPR007012. PolA_pol_cen_dom.
    IPR007010. PolA_pol_RNA-bd_dom.
    IPR014492. PolyA_polymerase.
    [Graphical view]
    PfamiPF01909. NTP_transf_2. 1 hit.
    PF04928. PAP_central. 1 hit.
    PF04926. PAP_RNA-bind. 1 hit.
    [Graphical view]
    PIRSFiPIRSF018425. PolyA_polymerase. 1 hit.
    SUPFAMiSSF55003. SSF55003. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9WVP6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPFAVTTQGA QQPAPAPKQF GISSPISLAA PKDTDRELTQ KLIETLQPFG    50
    VFEEEEELQR RILILQKLNN LVKEWIREIS ESRNLPQAVI ENVGGKIFTF 100
    GSYRLGVHTK GADIDALCVA PRHVDRNDFF TSFYDKLKLQ EEVKDLRAVE 150
    EAFVPVIKLC FDGIEIDILF ARLALQTIPE DLDLRDDSLL KNLDIRCIRS 200
    LNGCRVTDEI LHLVPNIDSF RLTLRAIKLW AKCHNIYSNI LGFLGGVSWA 250
    MLVARTCQLY PNAIASTLVR KFFLVFSEWE WPNPVLLKEP EERNLNLPVW 300
    DPRVNPSDRY HLMPIITPAY PQQNSTYNVS VSTRMVMIEE FKQGLAITHE 350
    ILLNKAEWSK LFEAPSFFQK YKHYIVLLAS APTEKQHLEW VGLVESKIRI 400
    LVGSLEKNEF ITLAHVNPQS FPAPKETADK EEFRTMWVIG LVLKKPENSE 450
    ILSIDLTYDI QSFTDTVYRQ AINSKMFEMD MKIAAMHLRR KELHQLLPNH 500
    VLQKKETHLT ESVRLTAVTD SSLLLSIDSE NSMTAPSPTG TMKTGPLTGN 550
    PQGRNSPALA VMAASVTNIQ FPDVSLQHVN PIESSGIALS ESIPQIPSQP 600
    TISPPPKPTM TRVVSSTHLV NHPSRPSGNT ATNIPNPILG V 641
    Length:641
    Mass (Da):72,198
    Last modified:September 26, 2001 - v2
    Checksum:iE10EE49B140A1086
    GO

    Sequence cautioni

    The sequence BAA83086.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti201 – 2011L → F in AAD43624. (PubMed:11150526)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF039957 mRNA. Translation: AAD43624.1.
    AB030729 mRNA. Translation: BAA83086.1. Different initiation.
    RefSeqiNP_064327.1. NM_019943.2.
    UniGeneiMm.441372.

    Genome annotation databases

    GeneIDi56522.
    KEGGimmu:56522.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF039957 mRNA. Translation: AAD43624.1 .
    AB030729 mRNA. Translation: BAA83086.1 . Different initiation.
    RefSeqi NP_064327.1. NM_019943.2.
    UniGenei Mm.441372.

    3D structure databases

    ProteinModelPortali Q9WVP6.
    SMRi Q9WVP6. Positions 19-498.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 208033. 1 interaction.
    IntActi Q9WVP6. 3 interactions.
    MINTi MINT-6167909.
    STRINGi 10090.ENSMUSP00000100595.

    PTM databases

    PhosphoSitei Q9WVP6.

    Proteomic databases

    PaxDbi Q9WVP6.
    PRIDEi Q9WVP6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 56522.
    KEGGi mmu:56522.

    Organism-specific databases

    CTDi 56903.
    MGIi MGI:1932115. Papolb.

    Phylogenomic databases

    eggNOGi COG5186.
    HOVERGENi HBG053502.
    InParanoidi Q9WVP6.
    KOi K14376.
    OMAi MSDGMAR.

    Miscellaneous databases

    NextBioi 312850.
    PROi Q9WVP6.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9WVP6.
    CleanExi MM_PAPOLB.
    Genevestigatori Q9WVP6.

    Family and domain databases

    Gene3Di 3.30.70.590. 1 hit.
    InterProi IPR002934. Nucleotidyltransferase.
    IPR011068. NuclTrfase_I_C.
    IPR007012. PolA_pol_cen_dom.
    IPR007010. PolA_pol_RNA-bd_dom.
    IPR014492. PolyA_polymerase.
    [Graphical view ]
    Pfami PF01909. NTP_transf_2. 1 hit.
    PF04928. PAP_central. 1 hit.
    PF04926. PAP_RNA-bind. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF018425. PolyA_polymerase. 1 hit.
    SUPFAMi SSF55003. SSF55003. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "An intronless gene encoding a poly(A) polymerase is specifically expressed in testis."
      Lee Y.J., Lee Y., Chung J.H.
      FEBS Lett. 487:287-292(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: BALB/c.
      Tissue: Testis.
    2. "Identification of a novel isoform of poly(A) polymerase, TPAP, specifically present in the cytoplasm of spermatogenic cells."
      Kashiwabara S., Zhuang T., Yamagata K., Noguchi J., Fukamizu A., Baba T.
      Dev. Biol. 228:106-115(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
      Strain: ddY.
      Tissue: Testis.
    3. "Germ cell-specific gene 1 targets testis-specific poly(A) polymerase to the endoplasmic reticulum through protein-protein interactions."
      Choi H.-S., Lee S.-H., Kim H., Lee Y.
      FEBS Lett. 582:1203-1209(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GSG1.

    Entry informationi

    Entry nameiPAPOB_MOUSE
    AccessioniPrimary (citable) accession number: Q9WVP6
    Secondary accession number(s): Q9R1R3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: September 26, 2001
    Last modified: October 1, 2014
    This is version 112 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3