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Q9WVP1

- AP1M2_MOUSE

UniProt

Q9WVP1 - AP1M2_MOUSE

Protein

AP-1 complex subunit mu-2

Gene

Ap1m2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

    GO - Biological processi

    1. intracellular protein transport Source: MGI
    2. vesicle-mediated transport Source: MGI

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_196550. MHC class II antigen presentation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    AP-1 complex subunit mu-2
    Alternative name(s):
    AP-mu chain family member mu1B
    Adaptor protein complex AP-1 subunit mu-2
    Adaptor-related protein complex 1 subunit mu-2
    Clathrin assembly protein complex 1 mu-2 medium chain 2
    Golgi adaptor HA1/AP1 adaptin mu-2 subunit
    Mu-adaptin 2
    Mu1B-adaptin
    Gene namesi
    Name:Ap1m2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:1336974. Ap1m2.

    Subcellular locationi

    Golgi apparatus. Cytoplasmic vesicleclathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side
    Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.

    GO - Cellular componenti

    1. clathrin adaptor complex Source: InterPro
    2. clathrin-coated vesicle membrane Source: UniProtKB-SubCell
    3. trans-Golgi network Source: MGI

    Keywords - Cellular componenti

    Cytoplasmic vesicle, Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 423423AP-1 complex subunit mu-2PRO_0000193773Add
    BLAST

    Post-translational modificationi

    Phosphorylation of membrane-bound AP1M1/AP1M2 increases its affinity for sorting signals.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9WVP1.
    PaxDbiQ9WVP1.
    PRIDEiQ9WVP1.

    PTM databases

    PhosphoSiteiQ9WVP1.

    Expressioni

    Gene expression databases

    BgeeiQ9WVP1.
    CleanExiMM_AP1M2.
    GenevestigatoriQ9WVP1.

    Interactioni

    Subunit structurei

    Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3).1 Publication

    Protein-protein interaction databases

    IntActiQ9WVP1. 1 interaction.
    MINTiMINT-4087927.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9WVP1.
    SMRiQ9WVP1. Positions 2-422.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini168 – 421254MHDPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 MHD (mu homology) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG315786.
    GeneTreeiENSGT00530000062779.
    HOGENOMiHOG000173247.
    HOVERGENiHBG050516.
    KOiK12393.
    OrthoDBiEOG783MV6.
    PhylomeDBiQ9WVP1.
    TreeFamiTF300393.

    Family and domain databases

    InterProiIPR022775. AP_mu_sigma_su.
    IPR001392. Clathrin_mu.
    IPR008968. Clathrin_mu_C.
    IPR018240. Clathrin_mu_CS.
    IPR011012. Longin-like_dom.
    IPR028565. MHD.
    [Graphical view]
    PfamiPF00928. Adap_comp_sub. 1 hit.
    PF01217. Clat_adaptor_s. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005992. Clathrin_mu. 1 hit.
    PRINTSiPR00314. CLATHRINADPT.
    SUPFAMiSSF49447. SSF49447. 1 hit.
    SSF64356. SSF64356. 1 hit.
    PROSITEiPS00990. CLAT_ADAPTOR_M_1. 1 hit.
    PS00991. CLAT_ADAPTOR_M_2. 1 hit.
    PS51072. MHD. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9WVP1-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSASAVFILD VKGKPLISRN YKGDVPMTEI DHFMPLLMQR EEEGVLAPLL    50
    SHGRVHFLWI KHSNLYLVAT TLKNANASLV YSFLYKTVEV FCEYFKELEE 100
    ESIRDNFVIV YDLLDELMDF GFPQTTDSKI LQEYITQQGN KLETGKSRVP 150
    PTVTNAVSWR SEGIKYKKNE VFIDVIESVN LLVNANGSVL LSEIVGTIKL 200
    KVFLSGMPEL RLGLNDRVLF ELTGRSKNKS VELEDVKFHQ CVRLSRFDND 250
    RTISFIPPDG DFELMSYRLS TQVKPLIWIE SVIEKFSHSR VEIMVKAKGQ 300
    FKKQSVANGV EISVPVPSDA DSPRFKTSVG SAKYVPEKNV VIWSIKSFPG 350
    GKEYLMRAHF GLPSVETEEV EGRPPIGVKF EIPYFTVSGI QVRYMKIIEK 400
    SGYQALPWVR YITQSGDYQL RTS 423
    Length:423
    Mass (Da):48,137
    Last modified:January 23, 2007 - v3
    Checksum:iFD18049309F074B5
    GO
    Isoform 2 (identifier: Q9WVP1-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         225-225: R → LSG

    Note: No experimental confirmation available.

    Show »
    Length:425
    Mass (Da):48,238
    Checksum:i094BC463C49B7D87
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti112 – 1121D → E in BAB26971. (PubMed:16141072)Curated
    Sequence conflicti112 – 1121D → E in AAH03704. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei225 – 2251R → LSG in isoform 2. 1 PublicationVSP_000170

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF067146 mRNA. Translation: AAD28085.1.
    AF139416
    , AF139406, AF139407, AF139408, AF139409, AF139410, AF139411, AF139412, AF139413, AF139414, AF139415 Genomic DNA. Translation: AAF61815.1.
    AK010478 mRNA. Translation: BAB26971.1.
    BC003704 mRNA. Translation: AAH03704.1.
    CCDSiCCDS22902.1. [Q9WVP1-1]
    CCDS52734.1. [Q9WVP1-2]
    RefSeqiNP_001103770.1. NM_001110300.1.
    NP_033808.2. NM_009678.2.
    UniGeneiMm.22239.

    Genome annotation databases

    EnsembliENSMUST00000003397; ENSMUSP00000003397; ENSMUSG00000003309.
    ENSMUST00000115433; ENSMUSP00000111093; ENSMUSG00000003309.
    GeneIDi11768.
    KEGGimmu:11768.
    UCSCiuc009okx.2. mouse. [Q9WVP1-1]
    uc009oky.2. mouse. [Q9WVP1-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF067146 mRNA. Translation: AAD28085.1 .
    AF139416
    , AF139406 , AF139407 , AF139408 , AF139409 , AF139410 , AF139411 , AF139412 , AF139413 , AF139414 , AF139415 Genomic DNA. Translation: AAF61815.1 .
    AK010478 mRNA. Translation: BAB26971.1 .
    BC003704 mRNA. Translation: AAH03704.1 .
    CCDSi CCDS22902.1. [Q9WVP1-1 ]
    CCDS52734.1. [Q9WVP1-2 ]
    RefSeqi NP_001103770.1. NM_001110300.1.
    NP_033808.2. NM_009678.2.
    UniGenei Mm.22239.

    3D structure databases

    ProteinModelPortali Q9WVP1.
    SMRi Q9WVP1. Positions 2-422.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9WVP1. 1 interaction.
    MINTi MINT-4087927.

    PTM databases

    PhosphoSitei Q9WVP1.

    Proteomic databases

    MaxQBi Q9WVP1.
    PaxDbi Q9WVP1.
    PRIDEi Q9WVP1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000003397 ; ENSMUSP00000003397 ; ENSMUSG00000003309 .
    ENSMUST00000115433 ; ENSMUSP00000111093 ; ENSMUSG00000003309 .
    GeneIDi 11768.
    KEGGi mmu:11768.
    UCSCi uc009okx.2. mouse. [Q9WVP1-1 ]
    uc009oky.2. mouse. [Q9WVP1-2 ]

    Organism-specific databases

    CTDi 10053.
    MGIi MGI:1336974. Ap1m2.

    Phylogenomic databases

    eggNOGi NOG315786.
    GeneTreei ENSGT00530000062779.
    HOGENOMi HOG000173247.
    HOVERGENi HBG050516.
    KOi K12393.
    OrthoDBi EOG783MV6.
    PhylomeDBi Q9WVP1.
    TreeFami TF300393.

    Enzyme and pathway databases

    Reactomei REACT_196550. MHC class II antigen presentation.

    Miscellaneous databases

    NextBioi 279537.
    PROi Q9WVP1.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9WVP1.
    CleanExi MM_AP1M2.
    Genevestigatori Q9WVP1.

    Family and domain databases

    InterProi IPR022775. AP_mu_sigma_su.
    IPR001392. Clathrin_mu.
    IPR008968. Clathrin_mu_C.
    IPR018240. Clathrin_mu_CS.
    IPR011012. Longin-like_dom.
    IPR028565. MHD.
    [Graphical view ]
    Pfami PF00928. Adap_comp_sub. 1 hit.
    PF01217. Clat_adaptor_s. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005992. Clathrin_mu. 1 hit.
    PRINTSi PR00314. CLATHRINADPT.
    SUPFAMi SSF49447. SSF49447. 1 hit.
    SSF64356. SSF64356. 1 hit.
    PROSITEi PS00990. CLAT_ADAPTOR_M_1. 1 hit.
    PS00991. CLAT_ADAPTOR_M_2. 1 hit.
    PS51072. MHD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
    2. "Genomic structure and chromosome mapping of the genes encoding clathrin-associated adaptor medium chains mu1A (Ap1m1) and mu1B (Ap1m2)."
      Nakatsu F., Kadohira T., Gilbert D.J., Jenkins N.A., Kakuta H., Copeland N.G., Saito T., Ohno H.
      Cytogenet. Cell Genet. 87:53-58(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6J.
      Tissue: Embryonic stem cell.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Mammary gland.
    5. "The polarized epithelia-specific mu 1B-adaptin complements mu 1A-deficiency in fibroblasts."
      Eskelinen E.L., Meyer C., Ohno H., von Figura K., Schu P.
      EMBO Rep. 3:471-477(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE AP-1 COMPLEX.

    Entry informationi

    Entry nameiAP1M2_MOUSE
    AccessioniPrimary (citable) accession number: Q9WVP1
    Secondary accession number(s): Q99LA4, Q9CWP7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 112 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3