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Protein

AP-1 complex subunit mu-2

Gene

Ap1m2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

GO - Biological processi

  • intracellular protein transport Source: MGI
  • vesicle-mediated transport Source: MGI

Keywordsi

Biological processProtein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
AP-1 complex subunit mu-2
Alternative name(s):
AP-mu chain family member mu1B
Adaptor protein complex AP-1 subunit mu-2
Adaptor-related protein complex 1 subunit mu-2
Clathrin assembly protein complex 1 mu-2 medium chain 2
Golgi adaptor HA1/AP1 adaptin mu-2 subunit
Mu-adaptin 2
Mu1B-adaptin
Gene namesi
Name:Ap1m2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1336974. Ap1m2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001937731 – 423AP-1 complex subunit mu-2Add BLAST423

Post-translational modificationi

Phosphorylation of membrane-bound AP1M1/AP1M2 increases its affinity for sorting signals.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9WVP1.
PeptideAtlasiQ9WVP1.
PRIDEiQ9WVP1.

PTM databases

iPTMnetiQ9WVP1.
PhosphoSitePlusiQ9WVP1.

Expressioni

Gene expression databases

CleanExiMM_AP1M2.

Interactioni

Subunit structurei

Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3).1 Publication

Protein-protein interaction databases

IntActiQ9WVP1. 1 interactor.
MINTiMINT-4087927.
STRINGi10090.ENSMUSP00000111093.

Structurei

3D structure databases

ProteinModelPortaliQ9WVP1.
SMRiQ9WVP1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini168 – 421MHDPROSITE-ProRule annotationAdd BLAST254

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0937. Eukaryota.
ENOG410XPFS. LUCA.
HOGENOMiHOG000173247.
HOVERGENiHBG050516.
InParanoidiQ9WVP1.
KOiK12393.
PhylomeDBiQ9WVP1.
TreeFamiTF300393.

Family and domain databases

InterProiView protein in InterPro
IPR022775. AP_mu_sigma_su.
IPR001392. Clathrin_mu.
IPR018240. Clathrin_mu_CS.
IPR011012. Longin-like_dom.
IPR028565. MHD.
PfamiView protein in Pfam
PF00928. Adap_comp_sub. 1 hit.
PF01217. Clat_adaptor_s. 1 hit.
PIRSFiPIRSF005992. Clathrin_mu. 1 hit.
PRINTSiPR00314. CLATHRINADPT.
SUPFAMiSSF49447. SSF49447. 1 hit.
SSF64356. SSF64356. 1 hit.
PROSITEiView protein in PROSITE
PS00990. CLAT_ADAPTOR_M_1. 1 hit.
PS00991. CLAT_ADAPTOR_M_2. 1 hit.
PS51072. MHD. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9WVP1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSASAVFILD VKGKPLISRN YKGDVPMTEI DHFMPLLMQR EEEGVLAPLL
60 70 80 90 100
SHGRVHFLWI KHSNLYLVAT TLKNANASLV YSFLYKTVEV FCEYFKELEE
110 120 130 140 150
ESIRDNFVIV YDLLDELMDF GFPQTTDSKI LQEYITQQGN KLETGKSRVP
160 170 180 190 200
PTVTNAVSWR SEGIKYKKNE VFIDVIESVN LLVNANGSVL LSEIVGTIKL
210 220 230 240 250
KVFLSGMPEL RLGLNDRVLF ELTGRSKNKS VELEDVKFHQ CVRLSRFDND
260 270 280 290 300
RTISFIPPDG DFELMSYRLS TQVKPLIWIE SVIEKFSHSR VEIMVKAKGQ
310 320 330 340 350
FKKQSVANGV EISVPVPSDA DSPRFKTSVG SAKYVPEKNV VIWSIKSFPG
360 370 380 390 400
GKEYLMRAHF GLPSVETEEV EGRPPIGVKF EIPYFTVSGI QVRYMKIIEK
410 420
SGYQALPWVR YITQSGDYQL RTS
Length:423
Mass (Da):48,137
Last modified:January 23, 2007 - v3
Checksum:iFD18049309F074B5
GO
Isoform 2 (identifier: Q9WVP1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     225-225: R → LSG

Note: No experimental confirmation available.
Show »
Length:425
Mass (Da):48,238
Checksum:i094BC463C49B7D87
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti112D → E in BAB26971 (PubMed:16141072).Curated1
Sequence conflicti112D → E in AAH03704 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_000170225R → LSG in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF067146 mRNA. Translation: AAD28085.1.
AF139416
, AF139406, AF139407, AF139408, AF139409, AF139410, AF139411, AF139412, AF139413, AF139414, AF139415 Genomic DNA. Translation: AAF61815.1.
AK010478 mRNA. Translation: BAB26971.1.
BC003704 mRNA. Translation: AAH03704.1.
CCDSiCCDS22902.1. [Q9WVP1-1]
CCDS52734.1. [Q9WVP1-2]
RefSeqiNP_001103770.1. NM_001110300.1.
NP_033808.2. NM_009678.2.
UniGeneiMm.22239.

Genome annotation databases

GeneIDi11768.
KEGGimmu:11768.
UCSCiuc009okx.2. mouse. [Q9WVP1-1]
uc009oky.2. mouse. [Q9WVP1-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiAP1M2_MOUSE
AccessioniPrimary (citable) accession number: Q9WVP1
Secondary accession number(s): Q99LA4, Q9CWP7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: January 23, 2007
Last modified: August 30, 2017
This is version 133 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families