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Q9WVP1 (AP1M2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
AP-1 complex subunit mu-2
Alternative name(s):
AP-mu chain family member mu1B
Adaptor protein complex AP-1 subunit mu-2
Adaptor-related protein complex 1 subunit mu-2
Clathrin assembly protein complex 1 mu-2 medium chain 2
Golgi adaptor HA1/AP1 adaptin mu-2 subunit
Mu-adaptin 2
Mu1B-adaptin
Gene names
Name:Ap1m2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length423 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

Subunit structure

Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3). Ref.5

Subcellular location

Golgi apparatus. Cytoplasmic vesicleclathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.

Post-translational modification

Phosphorylation of membrane-bound AP1M1/AP1M2 increases its affinity for sorting signals By similarity.

Sequence similarities

Belongs to the adaptor complexes medium subunit family.

Contains 1 MHD (mu homology) domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9WVP1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9WVP1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     225-225: R → LSG
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 423423AP-1 complex subunit mu-2
PRO_0000193773

Regions

Domain168 – 421254MHD

Natural variations

Alternative sequence2251R → LSG in isoform 2.
VSP_000170

Experimental info

Sequence conflict1121D → E in BAB26971. Ref.3
Sequence conflict1121D → E in AAH03704. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: FD18049309F074B5

FASTA42348,137
        10         20         30         40         50         60 
MSASAVFILD VKGKPLISRN YKGDVPMTEI DHFMPLLMQR EEEGVLAPLL SHGRVHFLWI 

        70         80         90        100        110        120 
KHSNLYLVAT TLKNANASLV YSFLYKTVEV FCEYFKELEE ESIRDNFVIV YDLLDELMDF 

       130        140        150        160        170        180 
GFPQTTDSKI LQEYITQQGN KLETGKSRVP PTVTNAVSWR SEGIKYKKNE VFIDVIESVN 

       190        200        210        220        230        240 
LLVNANGSVL LSEIVGTIKL KVFLSGMPEL RLGLNDRVLF ELTGRSKNKS VELEDVKFHQ 

       250        260        270        280        290        300 
CVRLSRFDND RTISFIPPDG DFELMSYRLS TQVKPLIWIE SVIEKFSHSR VEIMVKAKGQ 

       310        320        330        340        350        360 
FKKQSVANGV EISVPVPSDA DSPRFKTSVG SAKYVPEKNV VIWSIKSFPG GKEYLMRAHF 

       370        380        390        400        410        420 
GLPSVETEEV EGRPPIGVKF EIPYFTVSGI QVRYMKIIEK SGYQALPWVR YITQSGDYQL 


RTS 

« Hide

Isoform 2 [UniParc].

Checksum: 094BC463C49B7D87
Show »

FASTA42548,238

References

« Hide 'large scale' references
[1]"Mu1B, a novel adaptor medium chain expressed in polarized epithelial cells."
Ohno H., Tomemori T., Nakatsu F., Okazaki Y., Aguilar R.C., Foelsch H., Mellman I., Saito T., Shirasawa T., Bonifacino J.S.
FEBS Lett. 449:215-220(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: C57BL/6J.
[2]"Genomic structure and chromosome mapping of the genes encoding clathrin-associated adaptor medium chains mu1A (Ap1m1) and mu1B (Ap1m2)."
Nakatsu F., Kadohira T., Gilbert D.J., Jenkins N.A., Kakuta H., Copeland N.G., Saito T., Ohno H.
Cytogenet. Cell Genet. 87:53-58(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6J.
Tissue: Embryonic stem cell.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Mammary gland.
[5]"The polarized epithelia-specific mu 1B-adaptin complements mu 1A-deficiency in fibroblasts."
Eskelinen E.L., Meyer C., Ohno H., von Figura K., Schu P.
EMBO Rep. 3:471-477(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE AP-1 COMPLEX.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF067146 mRNA. Translation: AAD28085.1.
AF139416 expand/collapse EMBL AC list , AF139406, AF139407, AF139408, AF139409, AF139410, AF139411, AF139412, AF139413, AF139414, AF139415 Genomic DNA. Translation: AAF61815.1.
AK010478 mRNA. Translation: BAB26971.1.
BC003704 mRNA. Translation: AAH03704.1.
RefSeqNP_001103770.1. NM_001110300.1.
NP_033808.2. NM_009678.2.
UniGeneMm.22239.

3D structure databases

ProteinModelPortalQ9WVP1.
SMRQ9WVP1. Positions 2-422.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9WVP1. 1 interaction.
MINTMINT-4087927.

PTM databases

PhosphoSiteQ9WVP1.

Proteomic databases

PaxDbQ9WVP1.
PRIDEQ9WVP1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000003397; ENSMUSP00000003397; ENSMUSG00000003309.
ENSMUST00000115433; ENSMUSP00000111093; ENSMUSG00000003309.
GeneID11768.
KEGGmmu:11768.
UCSCuc009okx.2. mouse. [Q9WVP1-1]
uc009oky.2. mouse. [Q9WVP1-2]

Organism-specific databases

CTD10053.
MGIMGI:1336974. Ap1m2.

Phylogenomic databases

eggNOGNOG315786.
GeneTreeENSGT00530000062779.
HOGENOMHOG000173247.
HOVERGENHBG050516.
KOK12393.
OrthoDBEOG783MV6.
PhylomeDBQ9WVP1.
TreeFamTF300393.

Gene expression databases

BgeeQ9WVP1.
CleanExMM_AP1M2.
GenevestigatorQ9WVP1.

Family and domain databases

InterProIPR022775. AP_mu_sigma_su.
IPR001392. Clathrin_mu.
IPR008968. Clathrin_mu_C.
IPR018240. Clathrin_mu_CS.
IPR011012. Longin-like_dom.
IPR028565. MHD.
[Graphical view]
PfamPF00928. Adap_comp_sub. 1 hit.
PF01217. Clat_adaptor_s. 1 hit.
[Graphical view]
PIRSFPIRSF005992. Clathrin_mu. 1 hit.
PRINTSPR00314. CLATHRINADPT.
SUPFAMSSF49447. SSF49447. 1 hit.
SSF64356. SSF64356. 1 hit.
PROSITEPS00990. CLAT_ADAPTOR_M_1. 1 hit.
PS00991. CLAT_ADAPTOR_M_2. 1 hit.
PS51072. MHD. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio279537.
PROQ9WVP1.
SOURCESearch...

Entry information

Entry nameAP1M2_MOUSE
AccessionPrimary (citable) accession number: Q9WVP1
Secondary accession number(s): Q99LA4, Q9CWP7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 108 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot