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Q9WVP1

- AP1M2_MOUSE

UniProt

Q9WVP1 - AP1M2_MOUSE

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Protein

AP-1 complex subunit mu-2

Gene

Ap1m2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

GO - Biological processi

  1. intracellular protein transport Source: MGI
  2. vesicle-mediated transport Source: MGI
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiREACT_196550. MHC class II antigen presentation.

Names & Taxonomyi

Protein namesi
Recommended name:
AP-1 complex subunit mu-2
Alternative name(s):
AP-mu chain family member mu1B
Adaptor protein complex AP-1 subunit mu-2
Adaptor-related protein complex 1 subunit mu-2
Clathrin assembly protein complex 1 mu-2 medium chain 2
Golgi adaptor HA1/AP1 adaptin mu-2 subunit
Mu-adaptin 2
Mu1B-adaptin
Gene namesi
Name:Ap1m2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:1336974. Ap1m2.

Subcellular locationi

Golgi apparatus. Cytoplasmic vesicleclathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side
Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.

GO - Cellular componenti

  1. clathrin adaptor complex Source: InterPro
  2. cytoplasmic vesicle Source: UniProtKB-KW
  3. trans-Golgi network Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 423423AP-1 complex subunit mu-2PRO_0000193773Add
BLAST

Post-translational modificationi

Phosphorylation of membrane-bound AP1M1/AP1M2 increases its affinity for sorting signals.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9WVP1.
PaxDbiQ9WVP1.
PRIDEiQ9WVP1.

PTM databases

PhosphoSiteiQ9WVP1.

Expressioni

Gene expression databases

BgeeiQ9WVP1.
CleanExiMM_AP1M2.
GenevestigatoriQ9WVP1.

Interactioni

Subunit structurei

Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3).1 Publication

Protein-protein interaction databases

IntActiQ9WVP1. 1 interaction.
MINTiMINT-4087927.

Structurei

3D structure databases

ProteinModelPortaliQ9WVP1.
SMRiQ9WVP1. Positions 2-422.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini168 – 421254MHDPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 MHD (mu homology) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG315786.
GeneTreeiENSGT00530000062779.
HOGENOMiHOG000173247.
HOVERGENiHBG050516.
InParanoidiQ9WVP1.
KOiK12393.
OrthoDBiEOG783MV6.
PhylomeDBiQ9WVP1.
TreeFamiTF300393.

Family and domain databases

InterProiIPR022775. AP_mu_sigma_su.
IPR001392. Clathrin_mu.
IPR008968. Clathrin_mu_C.
IPR018240. Clathrin_mu_CS.
IPR011012. Longin-like_dom.
IPR028565. MHD.
[Graphical view]
PfamiPF00928. Adap_comp_sub. 1 hit.
PF01217. Clat_adaptor_s. 1 hit.
[Graphical view]
PIRSFiPIRSF005992. Clathrin_mu. 1 hit.
PRINTSiPR00314. CLATHRINADPT.
SUPFAMiSSF49447. SSF49447. 1 hit.
SSF64356. SSF64356. 1 hit.
PROSITEiPS00990. CLAT_ADAPTOR_M_1. 1 hit.
PS00991. CLAT_ADAPTOR_M_2. 1 hit.
PS51072. MHD. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9WVP1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSASAVFILD VKGKPLISRN YKGDVPMTEI DHFMPLLMQR EEEGVLAPLL
60 70 80 90 100
SHGRVHFLWI KHSNLYLVAT TLKNANASLV YSFLYKTVEV FCEYFKELEE
110 120 130 140 150
ESIRDNFVIV YDLLDELMDF GFPQTTDSKI LQEYITQQGN KLETGKSRVP
160 170 180 190 200
PTVTNAVSWR SEGIKYKKNE VFIDVIESVN LLVNANGSVL LSEIVGTIKL
210 220 230 240 250
KVFLSGMPEL RLGLNDRVLF ELTGRSKNKS VELEDVKFHQ CVRLSRFDND
260 270 280 290 300
RTISFIPPDG DFELMSYRLS TQVKPLIWIE SVIEKFSHSR VEIMVKAKGQ
310 320 330 340 350
FKKQSVANGV EISVPVPSDA DSPRFKTSVG SAKYVPEKNV VIWSIKSFPG
360 370 380 390 400
GKEYLMRAHF GLPSVETEEV EGRPPIGVKF EIPYFTVSGI QVRYMKIIEK
410 420
SGYQALPWVR YITQSGDYQL RTS
Length:423
Mass (Da):48,137
Last modified:January 23, 2007 - v3
Checksum:iFD18049309F074B5
GO
Isoform 2 (identifier: Q9WVP1-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     225-225: R → LSG

Note: No experimental confirmation available.

Show »
Length:425
Mass (Da):48,238
Checksum:i094BC463C49B7D87
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti112 – 1121D → E in BAB26971. (PubMed:16141072)Curated
Sequence conflicti112 – 1121D → E in AAH03704. (PubMed:15489334)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei225 – 2251R → LSG in isoform 2. 1 PublicationVSP_000170

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF067146 mRNA. Translation: AAD28085.1.
AF139416
, AF139406, AF139407, AF139408, AF139409, AF139410, AF139411, AF139412, AF139413, AF139414, AF139415 Genomic DNA. Translation: AAF61815.1.
AK010478 mRNA. Translation: BAB26971.1.
BC003704 mRNA. Translation: AAH03704.1.
CCDSiCCDS22902.1. [Q9WVP1-1]
CCDS52734.1. [Q9WVP1-2]
RefSeqiNP_001103770.1. NM_001110300.1.
NP_033808.2. NM_009678.2.
UniGeneiMm.22239.

Genome annotation databases

EnsembliENSMUST00000003397; ENSMUSP00000003397; ENSMUSG00000003309.
ENSMUST00000115433; ENSMUSP00000111093; ENSMUSG00000003309.
GeneIDi11768.
KEGGimmu:11768.
UCSCiuc009okx.2. mouse. [Q9WVP1-1]
uc009oky.2. mouse. [Q9WVP1-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF067146 mRNA. Translation: AAD28085.1 .
AF139416
, AF139406 , AF139407 , AF139408 , AF139409 , AF139410 , AF139411 , AF139412 , AF139413 , AF139414 , AF139415 Genomic DNA. Translation: AAF61815.1 .
AK010478 mRNA. Translation: BAB26971.1 .
BC003704 mRNA. Translation: AAH03704.1 .
CCDSi CCDS22902.1. [Q9WVP1-1 ]
CCDS52734.1. [Q9WVP1-2 ]
RefSeqi NP_001103770.1. NM_001110300.1.
NP_033808.2. NM_009678.2.
UniGenei Mm.22239.

3D structure databases

ProteinModelPortali Q9WVP1.
SMRi Q9WVP1. Positions 2-422.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9WVP1. 1 interaction.
MINTi MINT-4087927.

PTM databases

PhosphoSitei Q9WVP1.

Proteomic databases

MaxQBi Q9WVP1.
PaxDbi Q9WVP1.
PRIDEi Q9WVP1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000003397 ; ENSMUSP00000003397 ; ENSMUSG00000003309 .
ENSMUST00000115433 ; ENSMUSP00000111093 ; ENSMUSG00000003309 .
GeneIDi 11768.
KEGGi mmu:11768.
UCSCi uc009okx.2. mouse. [Q9WVP1-1 ]
uc009oky.2. mouse. [Q9WVP1-2 ]

Organism-specific databases

CTDi 10053.
MGIi MGI:1336974. Ap1m2.

Phylogenomic databases

eggNOGi NOG315786.
GeneTreei ENSGT00530000062779.
HOGENOMi HOG000173247.
HOVERGENi HBG050516.
InParanoidi Q9WVP1.
KOi K12393.
OrthoDBi EOG783MV6.
PhylomeDBi Q9WVP1.
TreeFami TF300393.

Enzyme and pathway databases

Reactomei REACT_196550. MHC class II antigen presentation.

Miscellaneous databases

NextBioi 279537.
PROi Q9WVP1.
SOURCEi Search...

Gene expression databases

Bgeei Q9WVP1.
CleanExi MM_AP1M2.
Genevestigatori Q9WVP1.

Family and domain databases

InterProi IPR022775. AP_mu_sigma_su.
IPR001392. Clathrin_mu.
IPR008968. Clathrin_mu_C.
IPR018240. Clathrin_mu_CS.
IPR011012. Longin-like_dom.
IPR028565. MHD.
[Graphical view ]
Pfami PF00928. Adap_comp_sub. 1 hit.
PF01217. Clat_adaptor_s. 1 hit.
[Graphical view ]
PIRSFi PIRSF005992. Clathrin_mu. 1 hit.
PRINTSi PR00314. CLATHRINADPT.
SUPFAMi SSF49447. SSF49447. 1 hit.
SSF64356. SSF64356. 1 hit.
PROSITEi PS00990. CLAT_ADAPTOR_M_1. 1 hit.
PS00991. CLAT_ADAPTOR_M_2. 1 hit.
PS51072. MHD. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
  2. "Genomic structure and chromosome mapping of the genes encoding clathrin-associated adaptor medium chains mu1A (Ap1m1) and mu1B (Ap1m2)."
    Nakatsu F., Kadohira T., Gilbert D.J., Jenkins N.A., Kakuta H., Copeland N.G., Saito T., Ohno H.
    Cytogenet. Cell Genet. 87:53-58(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Embryonic stem cell.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Mammary gland.
  5. "The polarized epithelia-specific mu 1B-adaptin complements mu 1A-deficiency in fibroblasts."
    Eskelinen E.L., Meyer C., Ohno H., von Figura K., Schu P.
    EMBO Rep. 3:471-477(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE AP-1 COMPLEX.

Entry informationi

Entry nameiAP1M2_MOUSE
AccessioniPrimary (citable) accession number: Q9WVP1
Secondary accession number(s): Q99LA4, Q9CWP7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 113 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3