Q9WVP1 (AP1M2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
AP-1 complex subunit mu-2

Alternative name(s):
AP-mu chain family member mu1B
Adaptor protein complex AP-1 mu-2 subunit
Adaptor-related protein complex 1 mu-2 subunit
Clathrin assembly protein complex 1 medium chain 2
Golgi adaptor HA1/AP1 adaptin mu-2 subunit
Mu-adaptin 2
Mu1B-adaptin

Gene names

Name:

Ap1m2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
Subunit structure	Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3). Ref.5
Subcellular location	Golgi apparatus. Cytoplasmic vesicle › clathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.
Post-translational modification	Phosphorylation of membrane-bound AP1M1/AP1M2 increases its affinity for sorting signals By similarity.
Sequence similarities	Belongs to the adaptor complexes medium subunit family. Contains 1 MHD (mu homology) domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasmic vesicle Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular protein transport Traceable author statement PubMed 9714600. Source: MGI vesicle-mediated transport Traceable author statement PubMed 9714600. Source: MGI
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell clathrin adaptor complex Inferred from electronic annotation. Source: InterPro clathrin-coated vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell trans-Golgi network Traceable author statement PubMed 9714600. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9WVP1-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9WVP1-2)

The sequence of this isoform differs from the canonical sequence as follows:
     225-225: R → LSG

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 423	423	AP-1 complex subunit mu-2		PRO_0000193773
Regions
	Domain	165 – 422	258	MHD
Natural variations
	Alternative sequence	225	1	R → LSG in isoform 2.		VSP_000170
Experimental info
	Sequence conflict	112	1	D → E in BAB26971. Ref.3
	Sequence conflict	112	1	D → E in AAH03704. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: FD18049309F074B5	FASTA	423	48,137
	10 20 30 40 50 60 MSASAVFILD VKGKPLISRN YKGDVPMTEI DHFMPLLMQR EEEGVLAPLL SHGRVHFLWI 70 80 90 100 110 120 KHSNLYLVAT TLKNANASLV YSFLYKTVEV FCEYFKELEE ESIRDNFVIV YDLLDELMDF 130 140 150 160 170 180 GFPQTTDSKI LQEYITQQGN KLETGKSRVP PTVTNAVSWR SEGIKYKKNE VFIDVIESVN 190 200 210 220 230 240 LLVNANGSVL LSEIVGTIKL KVFLSGMPEL RLGLNDRVLF ELTGRSKNKS VELEDVKFHQ 250 260 270 280 290 300 CVRLSRFDND RTISFIPPDG DFELMSYRLS TQVKPLIWIE SVIEKFSHSR VEIMVKAKGQ 310 320 330 340 350 360 FKKQSVANGV EISVPVPSDA DSPRFKTSVG SAKYVPEKNV VIWSIKSFPG GKEYLMRAHF 370 380 390 400 410 420 GLPSVETEEV EGRPPIGVKF EIPYFTVSGI QVRYMKIIEK SGYQALPWVR YITQSGDYQL RTS
	Isoform 2 [UniParc]. Checksum: 094BC463C49B7D87	FASTA	425	48,238
	10 20 30 40 50 60 MSASAVFILD VKGKPLISRN YKGDVPMTEI DHFMPLLMQR EEEGVLAPLL SHGRVHFLWI 70 80 90 100 110 120 KHSNLYLVAT TLKNANASLV YSFLYKTVEV FCEYFKELEE ESIRDNFVIV YDLLDELMDF 130 140 150 160 170 180 GFPQTTDSKI LQEYITQQGN KLETGKSRVP PTVTNAVSWR SEGIKYKKNE VFIDVIESVN 190 200 210 220 230 240 LLVNANGSVL LSEIVGTIKL KVFLSGMPEL RLGLNDRVLF ELTGLSGSKN KSVELEDVKF 250 260 270 280 290 300 HQCVRLSRFD NDRTISFIPP DGDFELMSYR LSTQVKPLIW IESVIEKFSH SRVEIMVKAK 310 320 330 340 350 360 GQFKKQSVAN GVEISVPVPS DADSPRFKTS VGSAKYVPEK NVVIWSIKSF PGGKEYLMRA 370 380 390 400 410 420 HFGLPSVETE EVEGRPPIGV KFEIPYFTVS GIQVRYMKII EKSGYQALPW VRYITQSGDY QLRTS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mu1B, a novel adaptor medium chain expressed in polarized epithelial cells." Ohno H., Tomemori T., Nakatsu F., Okazaki Y., Aguilar R.C., Foelsch H., Mellman I., Saito T., Shirasawa T., Bonifacino J.S. FEBS Lett. 449:215-220(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: C57BL/6J.
[2]	"Genomic structure and chromosome mapping of the genes encoding clathrin-associated adaptor medium chains mu1A (Ap1m1) and mu1B (Ap1m2)." Nakatsu F., Kadohira T., Gilbert D.J., Jenkins N.A., Kakuta H., Copeland N.G., Saito T., Ohno H. Cytogenet. Cell Genet. 87:53-58(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J. Tissue: Embryonic stem cell.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Mammary gland.
[5]	"The polarized epithelia-specific mu 1B-adaptin complements mu 1A-deficiency in fibroblasts." Eskelinen E.L., Meyer C., Ohno H., von Figura K., Schu P. EMBO Rep. 3:471-477(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE AP-1 COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF067146 mRNA. Translation: AAD28085.1. AF139416 , AF139406, AF139407, AF139408, AF139409, AF139410, AF139411, AF139412, AF139413, AF139414, AF139415 Genomic DNA. Translation: AAF61815.1. AK010478 mRNA. Translation: BAB26971.1. BC003704 mRNA. Translation: AAH03704.1.
IPI	IPI00126297. IPI00229147.
RefSeq	NP_001103770.1. NM_001110300.1. NP_033808.2. NM_009678.2.
UniGene	Mm.22239.
3D structure databases
ProteinModelPortal	Q9WVP1.
SMR	Q9WVP1. Positions 2-422.
ModBase	Search...
PTM databases
PhosphoSite	Q9WVP1.
Proteomic databases
PaxDb	Q9WVP1.
PRIDE	Q9WVP1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000003397; ENSMUSP00000003397; ENSMUSG00000003309. ENSMUST00000115433; ENSMUSP00000111093; ENSMUSG00000003309.
GeneID	11768.
KEGG	mmu:11768.
UCSC	uc009okx.2. mouse. uc009oky.2. mouse.
Organism-specific databases
CTD	10053.
MGI	MGI:1336974. Ap1m2.
Phylogenomic databases
eggNOG	NOG315786.
GeneTree	ENSGT00530000062779.
HOGENOM	HOG000173247.
HOVERGEN	HBG050516.
KO	K12393.
OrthoDB	EOG4CJVH3.
Gene expression databases
Bgee	Q9WVP1.
CleanEx	MM_AP1M2.
Genevestigator	Q9WVP1.
GermOnline	ENSMUSG00000003309. Mus musculus.
Family and domain databases
InterPro	IPR022775. AP_mu_sigma_su. IPR001392. Clathrin_mu. IPR008968. Clathrin_mu_C. IPR018240. Clathrin_mu_CS. IPR011012. Longin-like_dom. [Graphical view]
Pfam	PF00928. Adap_comp_sub. 1 hit. PF01217. Clat_adaptor_s. 1 hit. [Graphical view]
PIRSF	PIRSF005992. Clathrin_mu. 1 hit.
PRINTS	PR00314. CLATHRINADPT.
SUPFAM	SSF49447. AP50. 1 hit. SSF64356. Longin_like. 1 hit.
PROSITE	PS00990. CLAT_ADAPTOR_M_1. 1 hit. PS00991. CLAT_ADAPTOR_M_2. 1 hit. PS51072. MHD. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	279537.
SOURCE	Search...

Entry information

Entry name

AP1M2_MOUSE

Accession

Primary (citable) accession number: Q9WVP1
Secondary accession number(s): Q99LA4, Q9CWP7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 100 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program