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Protein

Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial

Gene

Aadat

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino-group acceptors, with a preference for 2-oxoglutarate, 2-oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro) (By similarity).By similarity

Catalytic activityi

L-kynurenine + 2-oxoglutarate = 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate.
L-2-aminoadipate + 2-oxoglutarate = 2-oxoadipate + L-glutamate.

Cofactori

Pathwayi: L-lysine degradation via saccharopine pathway

This protein is involved in step 4 of the subpathway that synthesizes glutaryl-CoA from L-lysine.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Alpha-aminoadipic semialdehyde synthase, mitochondrial (Aass)
  2. Alpha-aminoadipic semialdehyde synthase, mitochondrial (Aass)
  3. no protein annotated in this organism
  4. Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial (Aadat)
  5. no protein annotated in this organism
  6. Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial (Dlst)
This subpathway is part of the pathway L-lysine degradation via saccharopine pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutaryl-CoA from L-lysine, the pathway L-lysine degradation via saccharopine pathway and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei20SubstrateBy similarity1
Binding sitei74SubstrateBy similarity1
Binding sitei202SubstrateBy similarity1
Binding sitei399SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminotransferase, Transferase
LigandPyridoxal phosphate

Enzyme and pathway databases

BRENDAi2.6.1.39 3474
2.6.1.7 3474
ReactomeiR-MMU-71064 Lysine catabolism
R-MMU-71240 Tryptophan catabolism
UniPathwayiUPA00868; UER00838

Names & Taxonomyi

Protein namesi
Recommended name:
Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial
Short name:
KAT/AadAT
Alternative name(s):
2-aminoadipate aminotransferase
2-aminoadipate transaminase (EC:2.6.1.39)
Alpha-aminoadipate aminotransferase
Short name:
AadAT
Kynurenine aminotransferase II
Kynurenine--oxoglutarate aminotransferase II
Kynurenine--oxoglutarate transaminase 2 (EC:2.6.1.7)
Kynurenine--oxoglutarate transaminase II
Gene namesi
Name:Aadat
Synonyms:Kat2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1345167 Aadat

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 29MitochondrionSequence analysisAdd BLAST29
ChainiPRO_000002060330 – 425Kynurenine/alpha-aminoadipate aminotransferase, mitochondrialAdd BLAST396

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei40PhosphoserineCombined sources1
Modified residuei69N6-acetyllysineCombined sources1
Modified residuei172N6-succinyllysineCombined sources1
Modified residuei179N6-acetyllysineCombined sources1
Modified residuei263N6-(pyridoxal phosphate)lysine; alternateBy similarity1
Modified residuei263N6-acetyllysine; alternateCombined sources1
Modified residuei263N6-succinyllysine; alternateCombined sources1
Modified residuei339N6-acetyllysine; alternateCombined sources1
Modified residuei339N6-succinyllysine; alternateCombined sources1
Modified residuei351N6-acetyllysineCombined sources1
Modified residuei367N6-acetyllysine; alternateCombined sources1
Modified residuei367N6-succinyllysine; alternateCombined sources1
Modified residuei422N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9WVM8
PaxDbiQ9WVM8
PRIDEiQ9WVM8

PTM databases

iPTMnetiQ9WVM8
PhosphoSitePlusiQ9WVM8

Expressioni

Tissue specificityi

Expressed mainly in kidney and to a lesser amount in liver and brain.1 Publication

Gene expression databases

BgeeiENSMUSG00000057228
CleanExiMM_AADAT
ExpressionAtlasiQ9WVM8 baseline and differential
GenevisibleiQ9WVM8 MM

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9WVM8, 2 interactors
MINTiQ9WVM8
STRINGi10090.ENSMUSP00000078436

Structurei

3D structure databases

ProteinModelPortaliQ9WVM8
SMRiQ9WVM8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0634 Eukaryota
COG1167 LUCA
GeneTreeiENSGT00390000004594
HOGENOMiHOG000223057
HOVERGENiHBG050429
InParanoidiQ9WVM8
KOiK00825
OrthoDBiEOG091G0B8J
PhylomeDBiQ9WVM8
TreeFamiTF328598

Family and domain databases

Gene3Di3.40.640.10, 1 hit
InterProiView protein in InterPro
IPR004839 Aminotransferase_I/II
IPR015424 PyrdxlP-dep_Trfase
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF00155 Aminotran_1_2, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9WVM8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNYSRFLTAT SLARKPSPIR TTADILSKAP KTLISLAPGS PNPSMFPFKS
60 70 80 90 100
AAFTVENGST IRFEDDLIKR ALQYSPSYGI PELLSWLKQF QVKLHNPPTV
110 120 130 140 150
NYPPNQGQMD LCITSGCQDG LCKAFEMLIN PGDTILVNEP LFPGTLYAMK
160 170 180 190 200
PLGCNIINVP SDEHGIIPEG LKKILSQWKP EDSKDPTKKT PKFLYTVPNG
210 220 230 240 250
NNPTGNSLTG DRKKEIYELA RKYDFLIIED DPYYFLQFSK PWEPTFLSMD
260 270 280 290 300
VDGRVIRADT FSKTVSSGLR VGFMTGPKTL IQNIVLHTQV SSVHACTLSQ
310 320 330 340 350
LMILQLLHQW GEEGFLAHID RTIDFYKNQR DSILAAADKW LRGLAEWHVP
360 370 380 390 400
KAGMFLWIKV KGISDTKQLI EEKAIEREVL LVPGNGFFID GSAPTSFFRA
410 420
SFSLATPAQM DTAFQRLAQL IKESL
Length:425
Mass (Da):47,598
Last modified:November 1, 1999 - v1
Checksum:iE3A6711CFE96D77E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF072376 mRNA Translation: AAD39680.1
AK075578 mRNA Translation: BAC35833.1
BC012637 mRNA Translation: AAH12637.1
CCDSiCCDS22320.1
RefSeqiNP_035964.1, NM_011834.2
UniGeneiMm.35020

Genome annotation databases

EnsembliENSMUST00000079472; ENSMUSP00000078436; ENSMUSG00000057228
GeneIDi23923
KEGGimmu:23923
UCSCiuc009lte.1 mouse

Similar proteinsi

Entry informationi

Entry nameiAADAT_MOUSE
AccessioniPrimary (citable) accession number: Q9WVM8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: November 1, 1999
Last modified: May 23, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health