Q9WVM4 (ICMT_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein-S-isoprenylcysteine O-methyltransferase
EC=2.1.1.100

Alternative name(s):
Farnesyl cysteine carboxyl methyltransferase
Short name=FCMT
Isoprenylcysteine carboxylmethyltransferase
Prenylated protein carboxyl methyltransferase
Short name=PPMT
Prenylcysteine carboxyl methyltransferase
Short name=pcCMT

Gene names

Name:

Icmt

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	232 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues.
Catalytic activity	S-adenosyl-L-methionine + protein C-terminal S-farnesyl-L-cysteine = S-adenosyl-L-homocysteine + protein C-terminal S-farnesyl-L-cysteine methyl ester.
Cofactor	Divalent cations. Probably zinc.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the isoprenylcysteine O-methyltransferase family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	S-adenosylhomocysteine metabolic process Inferred from mutant phenotype PubMed 9192075. Source: RGD S-adenosylmethioninamine metabolic process Inferred from mutant phenotype PubMed 9192075. Source: RGD
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane Inferred from direct assay PubMed 9192075. Source: RGD
Molecular_function	protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity Inferred from mutant phenotype PubMed 9192075. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 232	›232	Protein-S-isoprenylcysteine O-methyltransferase		PRO_0000209896
Regions
	Transmembrane	‹1 – 7	›7	Helical; Potential
	Topological domain	8 – 17	10	Cytoplasmic Potential
	Transmembrane	18 – 35	18	Helical; Potential
	Topological domain	36 – 40	5	Lumenal Potential
	Transmembrane	41 – 60	20	Helical; Potential
	Topological domain	61 – 79	19	Cytoplasmic Potential
	Transmembrane	80 – 97	18	Helical; Potential
	Topological domain	98 – 102	5	Lumenal Potential
	Transmembrane	103 – 122	20	Helical; Potential
	Topological domain	123 – 160	38	Cytoplasmic Potential
	Transmembrane	161 – 176	16	Helical; Potential
	Topological domain	177	1	Lumenal Potential
	Transmembrane	178 – 192	15	Helical; Potential
	Topological domain	193 – 232	40	Cytoplasmic Potential
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Q9WVM4 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: BBB6D1CC14A8704E	FASTA	232	26,661
	10 20 30 40 50 60 ALLLLLYRPP HYQIAIRACF LGFVFGCGVL LSFSQSSWNH FGWYVCSLSL FHYSEYLVTT 70 80 90 100 110 120 VNNPKSLSLD SFLLNHSLEY TVAALSSWIE FTLENIFWPE LKQITWLSAA GLLMVIFGEC 130 140 150 160 170 180 LRKVAMFTAG SNFNHVVQSE KSDTHTLVTS GVYAWCRHPS YVGWFYWSIG TQVMLCNPIC 190 200 210 220 230 GVVYALTVWR FFRDRTEEEE ISLIHFFGEE YLDYKKRVPT GLPFIKGVKV GL

References

[1]	"Molecular cloning of farnesyl cysteine carboxyl methyltransferase gene from rat olfactory bulb." Otaki J.M., Firestein S. Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Olfactory bulb.
[2]	"The carboxyl methyltransferase modifying G proteins is a metalloenzyme." Desrosiers R.R., Nguyen Q.T., Beliveau R. Biochem. Biophys. Res. Commun. 261:790-797(1999) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION. Strain: Sprague-Dawley.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF075595 mRNA. Translation: AAD42926.1.
IPI	IPI00205177.
UniGene	Rn.48709.
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	Q9WVM4.
Proteomic databases
PRIDE	Q9WVM4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
UCSC	RGD:621618. rat.
Organism-specific databases
RGD	621618. Icmt.
Phylogenomic databases
eggNOG	COG2020.
HOGENOM	HOG000213961.
HOVERGEN	HBG019034.
InParanoid	Q9WVM4.
OrthoDB	EOG4NZTTX.
Enzyme and pathway databases
SABIO-RK	Q9WVM4.
Gene expression databases
ArrayExpress	Q9WVM4.
Genevestigator	Q9WVM4.
Family and domain databases
InterPro	IPR007269. ICMT_MeTrfase. IPR025770. PPMT_MeTrfase. [Graphical view]
Pfam	PF04140. ICMT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL2298.

Entry information

Entry name

ICMT_RAT

Accession

Primary (citable) accession number: Q9WVM4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	November 1, 1999
Last modified:	April 3, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program