Reviewed, UniProtKB/Swiss-Prot Q9WVL6 (EXTL3_MOUSE)

Names and origin

Protein names

Recommended name:
    Exostosin-like 3
    EC=2.4.1.223
Alternative name(s):
    Glucuronyl-galactosyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase
    Multiple exostosis-like protein 3

Gene names

Name:

Extl3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	918 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Probable glycosyltransferase By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->3)-beta-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-xylosyl-proteoglycan = UDP + alpha-N-acetyl-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-beta-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-xylosyl-proteoglycan.
Pathway	Glycan metabolism; heparan sulfate biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 47 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal-anchor Transmembrane
Ligand	Manganese Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell intrinsic to endoplasmic reticulum membrane Inferred from electronic annotation. Source: InterPro
Molecular function	glucuronyl-galactosyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 918	918	Exostosin-like 3		PRO_0000149658
Regions
	Topological domain	1 – 30	30	Cytoplasmic Potential
	Transmembrane	31 – 51	21	Signal-anchor for type II membrane protein Potential
	Topological domain	52 – 918	867	Lumenal Potential
Sites
	Metal binding	745	1	Manganese; catalytic By similarity
Amino acid modifications
	Glycosylation	277	1	N-linked (GlcNAc...) Potential
	Glycosylation	290	1	N-linked (GlcNAc...) Potential
	Glycosylation	591	1	N-linked (GlcNAc...) Potential
	Glycosylation	789	1	N-linked (GlcNAc...) Potential
	Disulfide bond	830 ↔ 878		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9WVL6-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 4EEFEC86E7E6027E	FASTA	918	104,548
	10 20 30 40 50 60 MTGYTMLRNG GVGNGGQTCM LRWSNRIRLT WLSFTLFIIL VFFPLIAHYY LTTLDEADEA 70 80 90 100 110 120 GKRIFGPRAG SELCEVKHVL DLCRIRESVS EELLQLEAKR QELNSEIAKL NLKIEACKKS 130 140 150 160 170 180 IENAKQDLLQ LKNVISQTEH SYKELMAQNQ PKLSLPIRLL PEKDDAGLPP PKVTRGCRLH 190 200 210 220 230 240 NCFDYSRCPL TSGFPVYVYD SDQFAFGSYL DPLVKQAFQA TVRANVYVTE NAAIACLYVV 250 260 270 280 290 300 LVGEMQEPTV LRPADLEKQL FSLPHWRTDG HNHVIINLSR KSDTQNLLYN VSTGRHVAQS 310 320 330 340 350 360 TLYAAQYRAG FDLVVSPLVH AMSEPNFMEI PPQVPVKRKY LFTFQGEKIE SLRSSLQEAR 370 380 390 400 410 420 SFEEEMEGDP PADYDDRIIA TLKAVQDSKL DQVLVEFTCK NQPKPSLPTE WALCGEREDR 430 440 450 460 470 480 LELLKLSTFA LIITPGDPRL LISSGCATRL FEALEVGAVP VVLGEQVQLP YHDMLQWNEA 490 500 510 520 530 540 ALVVPKPRVT EVHFLLRSLS DSDLLAMRRQ GRFLWETYFS TADSIFNTVL AMIRTRIQIP 550 560 570 580 590 600 AAPIREEVAA EIPHRSGKAA GTDPNMADNG DLDLGPVETE PPYASPKYLR NFTLTVTDCY 610 620 630 640 650 660 RGWNSAPGRF HLFPHTPFDP VLPSEAKFLG SGTGFRPIGG GAGGSGKEFQ AALGGNVQRE 670 680 690 700 710 720 QFTVVMLTYE REEVLMNSLE RLNGLPYLNK VVVVWNSPKL PSEDLLWPDI GVPIMVVRTE 730 740 750 760 770 780 KNSLNNRFLP WNEIETEAIL SIDDDAHLRH DEIMFGFWVW REARDRIVGF PGRYHAWDIP 790 800 810 820 830 840 HQSWLYNSNY SCELSMVLTG AAFFHKYYAY LYSYVMPQAI RDMVDEYINC EDIAMNFLVS 850 860 870 880 890 900 HITRKPPIKV TSRWTFRCPG CPQALSHDDS HFHERHKCIN FFVKVYGYMP LLYTQFRVDS 910 VLFKTRLPHD KTKCFKFI

References

[1]	"Molecular cloning of the mouse homolog of EXT1L." Sato T. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - GTase

EXTL3 (Putative GlcNAc Transferase I) [GAG Enzyme]

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF083550 mRNA. Translation: AAD42040.1.
IPI	IPI00126131.
UniGene	Mm.103748
3D structure databases
SMR	Q9WVL6. Positions 659-904.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9WVL6.
Protein family/group databases
CAZy	GT47. Glycosyltransferase Family 47. GT64. Glycosyltransferase Family 64.
Proteomic databases
PRIDE	Q9WVL6.
Genome annotation databases
Ensembl	ENSMUST00000022550; ENSMUSP00000022550; ENSMUSG00000021978; Mus musculus. [Genome view] ENSMUST00000111165; ENSMUSP00000106795; ENSMUSG00000021978; Mus musculus. [Genome view]
Organism-specific databases
MGI	MGI:1860765. Extl3.
Phylogenomic databases
HOVERGEN	Q9WVL6.
InParanoid	Q9WVL6.
Enzyme and pathway databases
BRENDA	2.4.1.223. 244.
Gene expression databases
ArrayExpress	Q9WVL6.
Bgee	Q9WVL6.
CleanEx	MM_EXTL3.
Genevestigator	Q9WVL6.
GermOnline	ENSMUSG00000021978. Mus musculus.
Family and domain databases
InterPro	IPR004263. Exostosin. IPR015338. HexNAc_Trfase_a. [Graphical view]
Pfam	PF03016. Exostosin. 1 hit. PF09258. Glyco_transf_64. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

EXTL3_MOUSE

Accession

Primary (citable) accession number: Q9WVL6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	November 1, 1999
Last modified:	January 19, 2010
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)