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Protein

Maleylacetoacetate isomerase

Gene

Gstz1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable bifunctional enzyme showing minimal glutathione-conjugating activity with ethacrynic acid and 7-chloro-4-nitrobenz-2-oxa-1, 3-diazole and maleylacetoacetate isomerase activity. Has also low glutathione peroxidase activity with t-butyl and cumene hydroperoxides. Is able to catalyze the glutathione dependent oxygenation of dichloroacetic acid to glyoxylic acid (By similarity).By similarity

Catalytic activityi

4-maleylacetoacetate = 4-fumarylacetoacetate.
RX + glutathione = HX + R-S-glutathione.

Cofactori

glutathioneBy similarityNote: Glutathione is required for the MAAI activity.By similarity

Pathwayi: L-phenylalanine degradation

This protein is involved in step 5 of the subpathway that synthesizes acetoacetate and fumarate from L-phenylalanine.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Phenylalanine-4-hydroxylase (Pah)
  2. Tyrosine aminotransferase (Tat)
  3. 4-hydroxyphenylpyruvate dioxygenase (Hpd)
  4. Homogentisate 1,2-dioxygenase (Hgd)
  5. Maleylacetoacetate isomerase (Gstz1)
  6. Fumarylacetoacetase (Fah)
This subpathway is part of the pathway L-phenylalanine degradation, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetoacetate and fumarate from L-phenylalanine, the pathway L-phenylalanine degradation and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei45Glutathione1 Publication1
Binding sitei59Glutathione; via amide nitrogen and carbonyl oxygen1 Publication1
Binding sitei111Glutathione1 Publication1

GO - Molecular functioni

GO - Biological processi

  • glutathione metabolic process Source: MGI
  • L-phenylalanine catabolic process Source: MGI
  • tyrosine catabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Transferase

Keywords - Biological processi

Phenylalanine catabolism, Tyrosine catabolism

Enzyme and pathway databases

ReactomeiR-MMU-156590. Glutathione conjugation.
R-MMU-71182. Phenylalanine and tyrosine catabolism.
UniPathwayiUPA00139; UER00340.

Names & Taxonomyi

Protein namesi
Recommended name:
Maleylacetoacetate isomerase (EC:5.2.1.2)
Short name:
MAAI
Alternative name(s):
GSTZ1-1
Glutathione S-transferase zeta 1 (EC:2.5.1.18)
Gene namesi
Name:Gstz1
Synonyms:Maai
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1341859. Gstz1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001860231 – 216Maleylacetoacetate isomeraseAdd BLAST216

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei57N6-succinyllysineCombined sources1
Modified residuei136PhosphothreonineCombined sources1
Modified residuei137PhosphoserineCombined sources1
Modified residuei177N6-succinyllysineCombined sources1
Modified residuei181PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9WVL0.
MaxQBiQ9WVL0.
PaxDbiQ9WVL0.
PeptideAtlasiQ9WVL0.
PRIDEiQ9WVL0.
TopDownProteomicsiQ9WVL0.

PTM databases

iPTMnetiQ9WVL0.
PhosphoSitePlusiQ9WVL0.
SwissPalmiQ9WVL0.

Expressioni

Tissue specificityi

Expressed in liver, kidney, seminal glands and breast.

Gene expression databases

BgeeiENSMUSG00000021033.
GenevisibleiQ9WVL0. MM.

Interactioni

Subunit structurei

Homodimer.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200105. 1 interactor.
IntActiQ9WVL0. 5 interactors.
MINTiMINT-1859246.
STRINGi10090.ENSMUSP00000053540.

Structurei

Secondary structure

1216
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 10Combined sources4
Helixi15 – 26Combined sources12
Beta strandi32 – 35Combined sources4
Helixi40 – 42Combined sources3
Helixi44 – 46Combined sources3
Helixi48 – 53Combined sources6
Beta strandi61 – 64Combined sources4
Beta strandi67 – 71Combined sources5
Helixi72 – 82Combined sources11
Helixi93 – 109Combined sources17
Helixi111 – 114Combined sources4
Helixi116 – 122Combined sources7
Turni124 – 126Combined sources3
Helixi127 – 149Combined sources23
Beta strandi150 – 156Combined sources7
Helixi161 – 175Combined sources15
Helixi184 – 194Combined sources11
Helixi197 – 200Combined sources4
Helixi204 – 206Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CZ2X-ray1.40A1-216[»]
2CZ3X-ray2.30A/B1-216[»]
ProteinModelPortaliQ9WVL0.
SMRiQ9WVL0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WVL0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 87GST N-terminalAdd BLAST84
Domaini92 – 212GST C-terminalAdd BLAST121

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 19Glutathione binding6
Regioni71 – 72Glutathione binding2
Regioni115 – 117Glutathione binding3

Sequence similaritiesi

Belongs to the GST superfamily. Zeta family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiKOG0868. Eukaryota.
COG0625. LUCA.
GeneTreeiENSGT00390000006580.
HOGENOMiHOG000125758.
HOVERGENiHBG001501.
InParanoidiQ9WVL0.
KOiK01800.
OMAiRAQVRMI.
OrthoDBiEOG091G0QFQ.
PhylomeDBiQ9WVL0.
TreeFamiTF105324.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR005955. Mal_ac_isom.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF14497. GST_C_3. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR01262. maiA. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WVL0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQAGKPILYS YFRSSCSWRV RIALALKGID YEIVPINLIK DGGQQFTEEF
60 70 80 90 100
QTLNPMKQVP ALKIDGITIV QSLAIMEYLE ETRPIPRLLP QDPQKRAIVR
110 120 130 140 150
MISDLIASGI QPLQNLSVLK QVGQENQMQW AQKVITSGFN ALEKILQSTA
160 170 180 190 200
GKYCVGDEVS MADVCLVPQV ANAERFKVDL SPYPTISHIN KELLALEVFQ
210
VSHPRRQPDT PAELRT
Length:216
Mass (Da):24,275
Last modified:November 1, 1999 - v1
Checksum:iBDB1E6C07981E855
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF093418 mRNA. Translation: AAD43846.1.
AK002398 mRNA. Translation: BAB22070.1.
AK075927 mRNA. Translation: BAC36059.1.
BC031777 mRNA. Translation: AAH31777.1.
CCDSiCCDS26071.1.
RefSeqiNP_001239484.1. NM_001252555.1.
NP_001239485.1. NM_001252556.1.
NP_034493.1. NM_010363.4.
UniGeneiMm.29652.

Genome annotation databases

EnsembliENSMUST00000063117; ENSMUSP00000053540; ENSMUSG00000021033.
GeneIDi14874.
KEGGimmu:14874.
UCSCiuc007oil.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF093418 mRNA. Translation: AAD43846.1.
AK002398 mRNA. Translation: BAB22070.1.
AK075927 mRNA. Translation: BAC36059.1.
BC031777 mRNA. Translation: AAH31777.1.
CCDSiCCDS26071.1.
RefSeqiNP_001239484.1. NM_001252555.1.
NP_001239485.1. NM_001252556.1.
NP_034493.1. NM_010363.4.
UniGeneiMm.29652.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CZ2X-ray1.40A1-216[»]
2CZ3X-ray2.30A/B1-216[»]
ProteinModelPortaliQ9WVL0.
SMRiQ9WVL0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200105. 1 interactor.
IntActiQ9WVL0. 5 interactors.
MINTiMINT-1859246.
STRINGi10090.ENSMUSP00000053540.

PTM databases

iPTMnetiQ9WVL0.
PhosphoSitePlusiQ9WVL0.
SwissPalmiQ9WVL0.

Proteomic databases

EPDiQ9WVL0.
MaxQBiQ9WVL0.
PaxDbiQ9WVL0.
PeptideAtlasiQ9WVL0.
PRIDEiQ9WVL0.
TopDownProteomicsiQ9WVL0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000063117; ENSMUSP00000053540; ENSMUSG00000021033.
GeneIDi14874.
KEGGimmu:14874.
UCSCiuc007oil.1. mouse.

Organism-specific databases

CTDi2954.
MGIiMGI:1341859. Gstz1.

Phylogenomic databases

eggNOGiKOG0868. Eukaryota.
COG0625. LUCA.
GeneTreeiENSGT00390000006580.
HOGENOMiHOG000125758.
HOVERGENiHBG001501.
InParanoidiQ9WVL0.
KOiK01800.
OMAiRAQVRMI.
OrthoDBiEOG091G0QFQ.
PhylomeDBiQ9WVL0.
TreeFamiTF105324.

Enzyme and pathway databases

UniPathwayiUPA00139; UER00340.
ReactomeiR-MMU-156590. Glutathione conjugation.
R-MMU-71182. Phenylalanine and tyrosine catabolism.

Miscellaneous databases

ChiTaRSiGstz1. mouse.
EvolutionaryTraceiQ9WVL0.
PROiQ9WVL0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021033.
GenevisibleiQ9WVL0. MM.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR005955. Mal_ac_isom.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF14497. GST_C_3. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR01262. maiA. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMAAI_MOUSE
AccessioniPrimary (citable) accession number: Q9WVL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1999
Last modified: November 2, 2016
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.