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Q9WVL0

- MAAI_MOUSE

UniProt

Q9WVL0 - MAAI_MOUSE

Protein

Maleylacetoacetate isomerase

Gene

Gstz1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Probable bifunctional enzyme showing minimal glutathione-conjugating activity with ethacrynic acid and 7-chloro-4-nitrobenz-2-oxa-1, 3-diazole and maleylacetoacetate isomerase activity. Has also low glutathione peroxidase activity with t-butyl and cumene hydroperoxides. Is able to catalyze the glutathione dependent oxygenation of dichloroacetic acid to glyoxylic acid By similarity.By similarity

    Catalytic activityi

    4-maleylacetoacetate = 4-fumarylacetoacetate.
    RX + glutathione = HX + R-S-glutathione.

    Cofactori

    Glutathione. Required for the MAAI activity By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei45 – 451Glutathione1 Publication
    Binding sitei59 – 591Glutathione; via amide nitrogen and carbonyl oxygen1 Publication
    Binding sitei111 – 1111Glutathione1 Publication

    GO - Molecular functioni

    1. glutathione transferase activity Source: MGI
    2. maleylacetoacetate isomerase activity Source: MGI

    GO - Biological processi

    1. glutathione metabolic process Source: Ensembl
    2. L-phenylalanine catabolic process Source: MGI
    3. tyrosine catabolic process Source: MGI

    Keywords - Molecular functioni

    Isomerase, Transferase

    Keywords - Biological processi

    Phenylalanine catabolism, Tyrosine catabolism

    Enzyme and pathway databases

    ReactomeiREACT_215316. Glutathione conjugation.
    UniPathwayiUPA00139; UER00340.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Maleylacetoacetate isomerase (EC:5.2.1.2)
    Short name:
    MAAI
    Alternative name(s):
    GSTZ1-1
    Glutathione S-transferase zeta 1 (EC:2.5.1.18)
    Gene namesi
    Name:Gstz1
    Synonyms:Maai
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 12

    Organism-specific databases

    MGIiMGI:1341859. Gstz1.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 216216Maleylacetoacetate isomerasePRO_0000186023Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei57 – 571N6-succinyllysine1 Publication
    Modified residuei136 – 1361Phosphothreonine1 Publication
    Modified residuei177 – 1771N6-succinyllysine1 Publication
    Modified residuei181 – 1811Phosphoserine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ9WVL0.
    PaxDbiQ9WVL0.
    PRIDEiQ9WVL0.

    PTM databases

    PhosphoSiteiQ9WVL0.

    Expressioni

    Tissue specificityi

    Expressed in liver, kidney, seminal glands and breast.

    Gene expression databases

    ArrayExpressiQ9WVL0.
    BgeeiQ9WVL0.
    GenevestigatoriQ9WVL0.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    IntActiQ9WVL0. 5 interactions.
    MINTiMINT-1859246.

    Structurei

    Secondary structure

    1
    216
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi7 – 104
    Helixi15 – 2612
    Beta strandi32 – 354
    Helixi40 – 423
    Helixi44 – 463
    Helixi48 – 536
    Beta strandi61 – 644
    Beta strandi67 – 715
    Helixi72 – 8211
    Helixi93 – 10917
    Helixi111 – 1144
    Helixi116 – 1227
    Turni124 – 1263
    Helixi127 – 14923
    Beta strandi150 – 1567
    Helixi161 – 17515
    Helixi184 – 19411
    Helixi197 – 2004
    Helixi204 – 2063

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2CZ2X-ray1.40A1-216[»]
    2CZ3X-ray2.30A/B1-216[»]
    ProteinModelPortaliQ9WVL0.
    SMRiQ9WVL0. Positions 4-214.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9WVL0.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 8784GST N-terminalAdd
    BLAST
    Domaini92 – 212121GST C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni14 – 196Glutathione binding
    Regioni71 – 722Glutathione binding
    Regioni115 – 1173Glutathione binding

    Sequence similaritiesi

    Belongs to the GST superfamily. Zeta family.Curated
    Contains 1 GST C-terminal domain.Curated
    Contains 1 GST N-terminal domain.Curated

    Phylogenomic databases

    eggNOGiCOG0625.
    GeneTreeiENSGT00390000006580.
    HOGENOMiHOG000125758.
    HOVERGENiHBG001501.
    InParanoidiQ9WVL0.
    KOiK01800.
    OMAiRAQVRMI.
    OrthoDBiEOG7TF79P.
    PhylomeDBiQ9WVL0.
    TreeFamiTF105324.

    Family and domain databases

    Gene3Di1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProiIPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR005955. Mal_ac_isom.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PfamiPF00043. GST_C. 1 hit.
    PF13417. GST_N_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    TIGRFAMsiTIGR01262. maiA. 1 hit.
    PROSITEiPS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9WVL0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQAGKPILYS YFRSSCSWRV RIALALKGID YEIVPINLIK DGGQQFTEEF    50
    QTLNPMKQVP ALKIDGITIV QSLAIMEYLE ETRPIPRLLP QDPQKRAIVR 100
    MISDLIASGI QPLQNLSVLK QVGQENQMQW AQKVITSGFN ALEKILQSTA 150
    GKYCVGDEVS MADVCLVPQV ANAERFKVDL SPYPTISHIN KELLALEVFQ 200
    VSHPRRQPDT PAELRT 216
    Length:216
    Mass (Da):24,275
    Last modified:November 1, 1999 - v1
    Checksum:iBDB1E6C07981E855
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF093418 mRNA. Translation: AAD43846.1.
    AK002398 mRNA. Translation: BAB22070.1.
    AK075927 mRNA. Translation: BAC36059.1.
    BC031777 mRNA. Translation: AAH31777.1.
    CCDSiCCDS26071.1.
    RefSeqiNP_001239484.1. NM_001252555.1.
    NP_001239485.1. NM_001252556.1.
    NP_034493.1. NM_010363.4.
    UniGeneiMm.29652.

    Genome annotation databases

    EnsembliENSMUST00000063117; ENSMUSP00000053540; ENSMUSG00000021033.
    GeneIDi14874.
    KEGGimmu:14874.
    UCSCiuc007oil.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF093418 mRNA. Translation: AAD43846.1 .
    AK002398 mRNA. Translation: BAB22070.1 .
    AK075927 mRNA. Translation: BAC36059.1 .
    BC031777 mRNA. Translation: AAH31777.1 .
    CCDSi CCDS26071.1.
    RefSeqi NP_001239484.1. NM_001252555.1.
    NP_001239485.1. NM_001252556.1.
    NP_034493.1. NM_010363.4.
    UniGenei Mm.29652.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2CZ2 X-ray 1.40 A 1-216 [» ]
    2CZ3 X-ray 2.30 A/B 1-216 [» ]
    ProteinModelPortali Q9WVL0.
    SMRi Q9WVL0. Positions 4-214.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9WVL0. 5 interactions.
    MINTi MINT-1859246.

    PTM databases

    PhosphoSitei Q9WVL0.

    Proteomic databases

    MaxQBi Q9WVL0.
    PaxDbi Q9WVL0.
    PRIDEi Q9WVL0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000063117 ; ENSMUSP00000053540 ; ENSMUSG00000021033 .
    GeneIDi 14874.
    KEGGi mmu:14874.
    UCSCi uc007oil.1. mouse.

    Organism-specific databases

    CTDi 2954.
    MGIi MGI:1341859. Gstz1.

    Phylogenomic databases

    eggNOGi COG0625.
    GeneTreei ENSGT00390000006580.
    HOGENOMi HOG000125758.
    HOVERGENi HBG001501.
    InParanoidi Q9WVL0.
    KOi K01800.
    OMAi RAQVRMI.
    OrthoDBi EOG7TF79P.
    PhylomeDBi Q9WVL0.
    TreeFami TF105324.

    Enzyme and pathway databases

    UniPathwayi UPA00139 ; UER00340 .
    Reactomei REACT_215316. Glutathione conjugation.

    Miscellaneous databases

    ChiTaRSi GSTZ1. mouse.
    EvolutionaryTracei Q9WVL0.
    NextBioi 287149.
    PROi Q9WVL0.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9WVL0.
    Bgeei Q9WVL0.
    Genevestigatori Q9WVL0.

    Family and domain databases

    Gene3Di 1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProi IPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR005955. Mal_ac_isom.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    Pfami PF00043. GST_C. 1 hit.
    PF13417. GST_N_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    TIGRFAMsi TIGR01262. maiA. 1 hit.
    PROSITEi PS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Gene structure, chromosomal location, and expression pattern of maleylacetoacetate isomerase."
      Fernandez-Canon J.M., Hejna J., Reifsteck C., Olson S., Grompe M.
      Genomics 58:263-269(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Kidney.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Salivary gland.
    4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-136 AND SER-181, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    5. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-57 AND LYS-177, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    6. "Crystal structure of glutathione transferase zeta 1-1 (maleylacetoacetate isomerase) from Mus musculus (form-1 crystal)."
      RIKEN structural genomics initiative (RSGI)
      Submitted (FEB-2009) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.4 ANGSTROMS) IN COMPLEX WITH GLUTATHIONE, SUBUNIT.

    Entry informationi

    Entry nameiMAAI_MOUSE
    AccessioniPrimary (citable) accession number: Q9WVL0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 131 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3