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Protein

Maleylacetoacetate isomerase

Gene

Gstz1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable bifunctional enzyme showing minimal glutathione-conjugating activity with ethacrynic acid and 7-chloro-4-nitrobenz-2-oxa-1, 3-diazole and maleylacetoacetate isomerase activity. Has also low glutathione peroxidase activity with t-butyl and cumene hydroperoxides. Is able to catalyze the glutathione dependent oxygenation of dichloroacetic acid to glyoxylic acid (By similarity).By similarity

Catalytic activityi

4-maleylacetoacetate = 4-fumarylacetoacetate.
RX + glutathione = HX + R-S-glutathione.

Cofactori

glutathioneBy similarityNote: Glutathione is required for the MAAI activity.By similarity

Pathwayi: L-phenylalanine degradation

This protein is involved in step 5 of the subpathway that synthesizes acetoacetate and fumarate from L-phenylalanine.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Phenylalanine-4-hydroxylase (Pah)
  2. Tyrosine aminotransferase (Tat)
  3. 4-hydroxyphenylpyruvate dioxygenase (Hpd)
  4. Homogentisate 1,2-dioxygenase (Hgd)
  5. Maleylacetoacetate isomerase (Gstz1)
  6. Fumarylacetoacetase (Fah)
This subpathway is part of the pathway L-phenylalanine degradation, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetoacetate and fumarate from L-phenylalanine, the pathway L-phenylalanine degradation and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei45Glutathione1 Publication1
Binding sitei59Glutathione; via amide nitrogen and carbonyl oxygen1 Publication1
Binding sitei111Glutathione1 Publication1

GO - Molecular functioni

GO - Biological processi

  • glutathione metabolic process Source: MGI
  • L-phenylalanine catabolic process Source: MGI
  • tyrosine catabolic process Source: MGI

Keywordsi

Molecular functionIsomerase, Multifunctional enzyme, Transferase
Biological processPhenylalanine catabolism, Tyrosine catabolism

Enzyme and pathway databases

ReactomeiR-MMU-156590 Glutathione conjugation
R-MMU-204174 Regulation of pyruvate dehydrogenase (PDH) complex
R-MMU-71182 Phenylalanine and tyrosine catabolism
UniPathwayiUPA00139; UER00340

Names & Taxonomyi

Protein namesi
Recommended name:
Maleylacetoacetate isomerase (EC:5.2.1.2)
Short name:
MAAI
Alternative name(s):
GSTZ1-1
Glutathione S-transferase zeta 1 (EC:2.5.1.18)
Gene namesi
Name:Gstz1
Synonyms:Maai
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1341859 Gstz1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001860231 – 216Maleylacetoacetate isomeraseAdd BLAST216

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei57N6-succinyllysineCombined sources1
Modified residuei136PhosphothreonineCombined sources1
Modified residuei137PhosphoserineCombined sources1
Modified residuei177N6-succinyllysineCombined sources1
Modified residuei181PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9WVL0
MaxQBiQ9WVL0
PaxDbiQ9WVL0
PeptideAtlasiQ9WVL0
PRIDEiQ9WVL0
TopDownProteomicsiQ9WVL0

PTM databases

iPTMnetiQ9WVL0
PhosphoSitePlusiQ9WVL0
SwissPalmiQ9WVL0

Expressioni

Tissue specificityi

Expressed in liver, kidney, seminal glands and breast.

Gene expression databases

BgeeiENSMUSG00000021033
ExpressionAtlasiQ9WVL0 baseline and differential
GenevisibleiQ9WVL0 MM

Interactioni

Subunit structurei

Homodimer.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200105, 1 interactor
IntActiQ9WVL0, 5 interactors
MINTiQ9WVL0
STRINGi10090.ENSMUSP00000053540

Structurei

Secondary structure

1216
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 10Combined sources4
Helixi15 – 26Combined sources12
Beta strandi32 – 35Combined sources4
Helixi40 – 42Combined sources3
Helixi44 – 46Combined sources3
Helixi48 – 53Combined sources6
Beta strandi61 – 64Combined sources4
Beta strandi67 – 71Combined sources5
Helixi72 – 82Combined sources11
Helixi93 – 109Combined sources17
Helixi111 – 114Combined sources4
Helixi116 – 122Combined sources7
Turni124 – 126Combined sources3
Helixi127 – 149Combined sources23
Beta strandi150 – 156Combined sources7
Helixi161 – 175Combined sources15
Helixi184 – 194Combined sources11
Helixi197 – 200Combined sources4
Helixi204 – 206Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CZ2X-ray1.40A1-216[»]
2CZ3X-ray2.30A/B1-216[»]
ProteinModelPortaliQ9WVL0
SMRiQ9WVL0
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WVL0

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 87GST N-terminalAdd BLAST84
Domaini92 – 212GST C-terminalAdd BLAST121

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni14 – 19Glutathione binding6
Regioni71 – 72Glutathione binding2
Regioni115 – 117Glutathione binding3

Sequence similaritiesi

Belongs to the GST superfamily. Zeta family.Curated

Phylogenomic databases

eggNOGiKOG0868 Eukaryota
COG0625 LUCA
GeneTreeiENSGT00390000006580
HOGENOMiHOG000125758
HOVERGENiHBG001501
InParanoidiQ9WVL0
KOiK01800
OMAiCRQPDTP
OrthoDBiEOG091G0QFQ
PhylomeDBiQ9WVL0
TreeFamiTF105324

Family and domain databases

CDDicd03191 GST_C_Zeta, 1 hit
cd03042 GST_N_Zeta, 1 hit
InterProiView protein in InterPro
IPR010987 Glutathione-S-Trfase_C-like
IPR036282 Glutathione-S-Trfase_C_sf
IPR004045 Glutathione_S-Trfase_N
IPR004046 GST_C
IPR005955 GST_Zeta
IPR034330 GST_Zeta_C
IPR034333 GST_Zeta_N
IPR036249 Thioredoxin-like_sf
PfamiView protein in Pfam
PF14497 GST_C_3, 1 hit
PF02798 GST_N, 1 hit
SUPFAMiSSF47616 SSF47616, 1 hit
SSF52833 SSF52833, 1 hit
TIGRFAMsiTIGR01262 maiA, 1 hit
PROSITEiView protein in PROSITE
PS50405 GST_CTER, 1 hit
PS50404 GST_NTER, 1 hit

Sequencei

Sequence statusi: Complete.

Q9WVL0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQAGKPILYS YFRSSCSWRV RIALALKGID YEIVPINLIK DGGQQFTEEF
60 70 80 90 100
QTLNPMKQVP ALKIDGITIV QSLAIMEYLE ETRPIPRLLP QDPQKRAIVR
110 120 130 140 150
MISDLIASGI QPLQNLSVLK QVGQENQMQW AQKVITSGFN ALEKILQSTA
160 170 180 190 200
GKYCVGDEVS MADVCLVPQV ANAERFKVDL SPYPTISHIN KELLALEVFQ
210
VSHPRRQPDT PAELRT
Length:216
Mass (Da):24,275
Last modified:November 1, 1999 - v1
Checksum:iBDB1E6C07981E855
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF093418 mRNA Translation: AAD43846.1
AK002398 mRNA Translation: BAB22070.1
AK075927 mRNA Translation: BAC36059.1
BC031777 mRNA Translation: AAH31777.1
CCDSiCCDS26071.1
RefSeqiNP_001239484.1, NM_001252555.1
NP_001239485.1, NM_001252556.1
NP_034493.1, NM_010363.4
UniGeneiMm.29652

Genome annotation databases

EnsembliENSMUST00000063117; ENSMUSP00000053540; ENSMUSG00000021033
GeneIDi14874
KEGGimmu:14874
UCSCiuc007oil.1 mouse

Similar proteinsi

Entry informationi

Entry nameiMAAI_MOUSE
AccessioniPrimary (citable) accession number: Q9WVL0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1999
Last modified: May 23, 2018
This is version 156 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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