Reviewed, UniProtKB/Swiss-Prot Q9WVK7 (HCDH_RAT)

Names and origin

Protein names

Recommended name:
    Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial
      Short name=HCDH
    EC=1.1.1.35
Alternative name(s):
    Short chain 3-hydroxyacyl-CoA dehydrogenase
    Medium and short chain L-3-hydroxyacyl-coenzyme A dehydrogenase

Gene names

Name:	Hadh
Synonyms:	Had, Hadhsc, Schad

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays an essential role in the mitochondrial beta-oxidation of short chain fatty acids. Exerts it highest activity toward 3-hydroxybutyryl-CoA.
Catalytic activity	(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.
Pathway	Lipid metabolism; fatty acid beta-oxidation.
Subunit structure	Homodimer By similarity.
Subcellular location	Mitochondrion matrix.
Sequence similarities	Belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Acetylation
Gene Ontology (GO)
Biological process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of insulin secretion Inferred from mutant phenotype. Source: RGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to activity Inferred from direct assay. Source: RGD response to drug Inferred from direct assay. Source: RGD response to insulin stimulus Inferred from direct assay. Source: RGD
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-hydroxyacyl-CoA dehydrogenase activity Inferred from electronic annotation. Source: EC coenzyme binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 12	12	Mitochondrion By similarity
	Chain	13 – 314	302	Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial		PRO_0000007409
Regions
	Nucleotide binding	34 – 39	6	NAD By similarity
Sites
	Binding site	57	1	NAD By similarity
	Binding site	73	1	Coenzyme A By similarity
	Binding site	80	1	Coenzyme A By similarity
	Binding site	122	1	NAD By similarity
	Binding site	127	1	NAD By similarity
	Binding site	149	1	Coenzyme A By similarity
	Binding site	149	1	NAD By similarity
	Binding site	173	1	NAD By similarity
	Binding site	305	1	NAD By similarity
	Site	170	1	Important for catalytic activity By similarity
Amino acid modifications
	Modified residue	179	1	N6-acetyllysine By similarity
	Modified residue	185	1	N6-acetyllysine By similarity
	Modified residue	202	1	N6-acetyllysine By similarity
	Modified residue	241	1	N6-acetyllysine By similarity
	Modified residue	312	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9WVK7-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: B36518766D6C3828	FASTA	314	34,448
	10 20 30 40 50 60 MAFVTRQFVR SMSSSSSASA AAKKILIKHV TVIGGGLMGA GIAQVAAATG HTVVLVDQTE 70 80 90 100 110 120 DILAKSKKGI EESLKRMAKK KFTENPKAAD EFVEKTLSSL STSTDAASVV HSTDLVVEAI 130 140 150 160 170 180 VENLKLKNEL FQRLDKFAAE HTIFASNTSS LQITNIANAT TRQDRFAGLH FFNPVPMMKL 190 200 210 220 230 240 VEVIKTPMTS QKTFESLVDF CKTLGKHPVS CKDTPGFIVN RLLVPYLIEA IRLHERGDAS 250 260 270 280 290 300 KEDIDTAMKL GAGYPMGPFE LLDYVGLDTT KFILDGWHEM DPENPLFQPS PSMNNLVAQK 310 KLGKKTGEGF YKYK

References

[1]	"Identity of heart and liver L-3-hydroxyacyl coenzyme A dehydrogenase." He X.-Y., Zhang G., Blecha F., Yang S.-Y. Biochim. Biophys. Acta 1437:119-123(1999) [PubMed: 10064895] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Heart muscle and Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF095449 mRNA. Translation: AAD42162.1.
IPI	IPI00205157.
PIR	S74114.
RefSeq	NP_476534.1.
UniGene	Rn.92789
3D structure databases
SMR	Q9WVK7. Positions 24-314.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9WVK7.
Proteomic databases
PRIDE	Q9WVK7.
Genome annotation databases
Ensembl	ENSRNOT00000014658; ENSRNOP00000014658; ENSRNOG00000010697; Rattus norvegicus. [Genome view]
GeneID	113965.
KEGG	rno:113965.
NMPDR	fig|10116.3.peg.17024.
UCSC	NM_057186. rat.
Organism-specific databases
CTD	113965.
RGD	69321. Hadh.
Phylogenomic databases
eggNOG	roNOG15739.
HOVERGEN	Q9WVK7.
InParanoid	Q9WVK7.
OMA	DTTKFIM.
OrthoDB	EOG9G7FJB.
PhylomeDB	Q9WVK7.
Enzyme and pathway databases
BRENDA	1.1.1.35. 248.
Gene expression databases
ArrayExpress	Q9WVK7.
Genevestigator	Q9WVK7.
GermOnline	ENSRNOG00000010697. Rattus norvegicus.
Family and domain databases
InterPro	IPR006180. 3-OHacyl-CoA_DH_CS. IPR006176. 3-OHacyl-CoA_DH_NAD-bd. IPR006108. 3HC_DH_C. IPR008927. 6-PGluconate_DH_C-like. IPR013328. DH_multihelical. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
Pfam	PF00725. 3HCDH. 1 hit. PF02737. 3HCDH_N. 1 hit. [Graphical view]
PROSITE	PS00067. 3HCDH. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	618105.

Entry information

Entry name

HCDH_RAT

Accession

Primary (citable) accession number: Q9WVK7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1999
Last modified:	January 19, 2010
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)