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Protein

Beta-1,4-galactosyltransferase 6

Gene

B4galt6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Required for the biosynthesis of glycosphingolipids.By similarity

Catalytic activityi

UDP-alpha-D-galactose + beta-D-glucosyl-(1<->1)-ceramide = UDP + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.By similarity

Cofactori

Mn2+By similarity, Mg2+By similarity, Ca2+By similarity

Enzyme regulationi

Inhibited by EDTA.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi230ManganeseBy similarity1
Binding sitei258UDP-alpha-D-galactoseBy similarity1
Binding sitei290UDP-alpha-D-galactoseBy similarity1
Metal bindingi323Manganese; via tele nitrogenBy similarity1
Binding sitei334N-acetyl-D-glucosamineBy similarity1

GO - Molecular functioni

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • protein glycosylation Source: UniProtKB-UniPathway
  • sphingolipid biosynthetic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Calcium, Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-2022854. Keratan sulfate biosynthesis.
R-MMU-975577. N-Glycan antennae elongation.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-galactosyltransferase 6
Short name:
Beta-1,4-GalTase 6
Short name:
Beta4Gal-T6
Short name:
b4Gal-T6
Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 6
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 6
Including the following 1 domains:
Glucosylceramide beta-1,4-galactosyltransferase (EC:2.4.1.274By similarity)
Alternative name(s):
Lactosylceramide synthase
Short name:
LacCer synthase
UDP-Gal:glucosylceramide beta-1,4-galactosyltransferase
Gene namesi
Name:B4galt6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1928380. B4galt6.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 15CytoplasmicSequence analysisAdd BLAST15
Transmembranei16 – 35Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST20
Topological domaini36 – 382LumenalSequence analysisAdd BLAST347

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000805481 – 382Beta-1,4-galactosyltransferase 6Add BLAST382

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi71N-linked (GlcNAc...)Sequence analysis1
Glycosylationi75N-linked (GlcNAc...)Sequence analysis1
Glycosylationi83N-linked (GlcNAc...)Sequence analysis1
Glycosylationi84N-linked (GlcNAc...)Sequence analysis1
Glycosylationi99N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi108 ↔ 152By similarity
Glycosylationi122N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi223 ↔ 242By similarity
Glycosylationi307N-linked (GlcNAc...)Sequence analysis1
Glycosylationi367N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9WVK5.
PRIDEiQ9WVK5.

PTM databases

PhosphoSitePlusiQ9WVK5.

Expressioni

Gene expression databases

BgeeiENSMUSG00000056124.
ExpressionAtlasiQ9WVK5. baseline and differential.
GenevisibleiQ9WVK5. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000066515.

Structurei

3D structure databases

ProteinModelPortaliQ9WVK5.
SMRiQ9WVK5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni163 – 167UDP-alpha-D-galactose bindingBy similarity5
Regioni202 – 204UDP-alpha-D-galactose bindingBy similarity3
Regioni229 – 230UDP-alpha-D-galactose bindingBy similarity2
Regioni292 – 295N-acetyl-D-glucosamine bindingBy similarity4
Regioni323 – 324UDP-alpha-D-galactose bindingBy similarity2

Sequence similaritiesi

Belongs to the glycosyltransferase 7 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3916. Eukaryota.
ENOG410ZYYA. LUCA.
GeneTreeiENSGT00760000119140.
HOGENOMiHOG000231027.
HOVERGENiHBG058334.
InParanoidiQ9WVK5.
KOiK07553.
OMAiMYLPENF.
OrthoDBiEOG091G0P66.
PhylomeDBiQ9WVK5.
TreeFamiTF312834.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9WVK5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSALKRMMRV SNRSLIAFIF FFSLSTSCLY FIYVAPGIAN TYLFMVQARG
60 70 80 90 100
IMLRENVKTI GHMIRLYTNK NTTLNGTDYP EGNNTSDYLV QTTTYLPQNF
110 120 130 140 150
TYLPHLPCPE KLPYMRGFLS VNVSEISFDE VHQLFSKDSE IGPGGHWRPK
160 170 180 190 200
DCKPRWKVAV LIPFRNRHEH LPIFFLHLIP MLQKQRLEFA FYVIEQTGTQ
210 220 230 240 250
PFNRAMLFNV GFKEAMKDRA WDCVIFHDVD HLPENDRNYY GCGEMPRHFA
260 270 280 290 300
AKLDKYMYIL PYKEFFGGVS GLTVEQFRKI NGFPNAFWGW GGEDDDLWNR
310 320 330 340 350
VHYAGYNVTR PEGDLGKYIS IPHHHRGEVQ FLGRYKLLRY SKERQYIDGL
360 370 380
NNLLYTPKIL VDRLYTNISV NLMPELAPIE DY
Length:382
Mass (Da):44,759
Last modified:November 1, 1999 - v1
Checksum:i0223621127896358
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF097158 mRNA. Translation: AAD41694.1.
AF142674 mRNA. Translation: AAF22224.1.
BC011149 mRNA. Translation: AAH11149.1.
CCDSiCCDS29086.1.
RefSeqiNP_062711.1. NM_019737.2.
UniGeneiMm.393827.

Genome annotation databases

EnsembliENSMUST00000070080; ENSMUSP00000066515; ENSMUSG00000056124.
GeneIDi56386.
KEGGimmu:56386.
UCSCiuc008eeu.2. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

b4GalT6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF097158 mRNA. Translation: AAD41694.1.
AF142674 mRNA. Translation: AAF22224.1.
BC011149 mRNA. Translation: AAH11149.1.
CCDSiCCDS29086.1.
RefSeqiNP_062711.1. NM_019737.2.
UniGeneiMm.393827.

3D structure databases

ProteinModelPortaliQ9WVK5.
SMRiQ9WVK5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000066515.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

PTM databases

PhosphoSitePlusiQ9WVK5.

Proteomic databases

PaxDbiQ9WVK5.
PRIDEiQ9WVK5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000070080; ENSMUSP00000066515; ENSMUSG00000056124.
GeneIDi56386.
KEGGimmu:56386.
UCSCiuc008eeu.2. mouse.

Organism-specific databases

CTDi9331.
MGIiMGI:1928380. B4galt6.

Phylogenomic databases

eggNOGiKOG3916. Eukaryota.
ENOG410ZYYA. LUCA.
GeneTreeiENSGT00760000119140.
HOGENOMiHOG000231027.
HOVERGENiHBG058334.
InParanoidiQ9WVK5.
KOiK07553.
OMAiMYLPENF.
OrthoDBiEOG091G0P66.
PhylomeDBiQ9WVK5.
TreeFamiTF312834.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-MMU-2022854. Keratan sulfate biosynthesis.
R-MMU-975577. N-Glycan antennae elongation.

Miscellaneous databases

PROiQ9WVK5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000056124.
ExpressionAtlasiQ9WVK5. baseline and differential.
GenevisibleiQ9WVK5. MM.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiB4GT6_MOUSE
AccessioniPrimary (citable) accession number: Q9WVK5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: November 1, 1999
Last modified: November 30, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.