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Q9WVK5

- B4GT6_MOUSE

UniProt

Q9WVK5 - B4GT6_MOUSE

Protein

Beta-1,4-galactosyltransferase 6

Gene

B4galt6

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Required for the biosynthesis of glycosphingolipids.By similarity

    Catalytic activityi

    UDP-alpha-D-galactose + beta-D-glucosyl-(1<->1)-ceramide = UDP + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.

    Cofactori

    Manganese or magnesium or calcium.By similarity

    Enzyme regulationi

    Inhibited by EDTA.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi230 – 2301ManganeseBy similarity
    Binding sitei258 – 2581UDP-alpha-D-galactoseBy similarity
    Binding sitei290 – 2901UDP-alpha-D-galactoseBy similarity
    Metal bindingi323 – 3231Manganese; via tele nitrogenBy similarity
    Binding sitei334 – 3341N-acetyl-D-glucosamineBy similarity

    GO - Molecular functioni

    1. galactosyltransferase activity Source: MGI
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein glycosylation Source: UniProtKB-UniPathway
    2. sphingolipid biosynthetic process Source: MGI

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Calcium, Magnesium, Manganese, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_198565. N-Glycan antennae elongation.
    REACT_198578. Keratan sulfate biosynthesis.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT7. Glycosyltransferase Family 7.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-1,4-galactosyltransferase 6 (EC:2.4.1.-)
    Short name:
    Beta-1,4-GalTase 6
    Short name:
    Beta4Gal-T6
    Short name:
    b4Gal-T6
    Alternative name(s):
    UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 6
    UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 6
    Including the following 1 domains:
    Glucosylceramide beta-1,4-galactosyltransferase (EC:2.4.1.274)
    Alternative name(s):
    Lactosylceramide synthase
    Short name:
    LacCer synthase
    UDP-Gal:glucosylceramide beta-1,4-galactosyltransferase
    Gene namesi
    Name:B4galt6
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 18

    Organism-specific databases

    MGIiMGI:1928380. B4galt6.

    Subcellular locationi

    Golgi apparatusGolgi stack membrane By similarity; Single-pass type II membrane protein By similarity
    Note: Trans cisternae of Golgi stack.By similarity

    GO - Cellular componenti

    1. Golgi cisterna membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 382382Beta-1,4-galactosyltransferase 6PRO_0000080548Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi71 – 711N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi75 – 751N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi83 – 831N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi84 – 841N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi99 – 991N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi108 ↔ 152By similarity
    Glycosylationi122 – 1221N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi223 ↔ 242By similarity
    Glycosylationi307 – 3071N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi367 – 3671N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ9WVK5.

    PTM databases

    PhosphoSiteiQ9WVK5.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9WVK5.
    BgeeiQ9WVK5.
    GenevestigatoriQ9WVK5.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000066515.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9WVK5.
    SMRiQ9WVK5. Positions 108-372.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1515CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini36 – 382347LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei16 – 3520Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni163 – 1675UDP-alpha-D-galactose bindingBy similarity
    Regioni202 – 2043UDP-alpha-D-galactose bindingBy similarity
    Regioni229 – 2302UDP-alpha-D-galactose bindingBy similarity
    Regioni292 – 2954N-acetyl-D-glucosamine bindingBy similarity
    Regioni323 – 3242UDP-alpha-D-galactose bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyltransferase 7 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG327897.
    GeneTreeiENSGT00750000117412.
    HOGENOMiHOG000231027.
    HOVERGENiHBG058334.
    InParanoidiQ9WVK5.
    KOiK07553.
    OMAiMYLPENF.
    OrthoDBiEOG7060R0.
    PhylomeDBiQ9WVK5.
    TreeFamiTF312834.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR003859. Galactosyl_T.
    IPR027791. Galactosyl_T_C.
    IPR027995. Galactosyl_T_N.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR19300. PTHR19300. 1 hit.
    PfamiPF02709. Glyco_transf_7C. 1 hit.
    PF13733. Glyco_transf_7N. 1 hit.
    [Graphical view]
    PRINTSiPR02050. B14GALTRFASE.
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9WVK5-1 [UniParc]FASTAAdd to Basket

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    MSALKRMMRV SNRSLIAFIF FFSLSTSCLY FIYVAPGIAN TYLFMVQARG    50
    IMLRENVKTI GHMIRLYTNK NTTLNGTDYP EGNNTSDYLV QTTTYLPQNF 100
    TYLPHLPCPE KLPYMRGFLS VNVSEISFDE VHQLFSKDSE IGPGGHWRPK 150
    DCKPRWKVAV LIPFRNRHEH LPIFFLHLIP MLQKQRLEFA FYVIEQTGTQ 200
    PFNRAMLFNV GFKEAMKDRA WDCVIFHDVD HLPENDRNYY GCGEMPRHFA 250
    AKLDKYMYIL PYKEFFGGVS GLTVEQFRKI NGFPNAFWGW GGEDDDLWNR 300
    VHYAGYNVTR PEGDLGKYIS IPHHHRGEVQ FLGRYKLLRY SKERQYIDGL 350
    NNLLYTPKIL VDRLYTNISV NLMPELAPIE DY 382
    Length:382
    Mass (Da):44,759
    Last modified:November 1, 1999 - v1
    Checksum:i0223621127896358
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF097158 mRNA. Translation: AAD41694.1.
    AF142674 mRNA. Translation: AAF22224.1.
    BC011149 mRNA. Translation: AAH11149.1.
    CCDSiCCDS29086.1.
    RefSeqiNP_062711.1. NM_019737.2.
    UniGeneiMm.393827.

    Genome annotation databases

    EnsembliENSMUST00000070080; ENSMUSP00000066515; ENSMUSG00000056124.
    GeneIDi56386.
    KEGGimmu:56386.
    UCSCiuc008eeu.2. mouse.

    Cross-referencesi

    Web resourcesi

    Functional Glycomics Gateway - GTase

    b4GalT6

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF097158 mRNA. Translation: AAD41694.1 .
    AF142674 mRNA. Translation: AAF22224.1 .
    BC011149 mRNA. Translation: AAH11149.1 .
    CCDSi CCDS29086.1.
    RefSeqi NP_062711.1. NM_019737.2.
    UniGenei Mm.393827.

    3D structure databases

    ProteinModelPortali Q9WVK5.
    SMRi Q9WVK5. Positions 108-372.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000066515.

    Protein family/group databases

    CAZyi GT7. Glycosyltransferase Family 7.

    PTM databases

    PhosphoSitei Q9WVK5.

    Proteomic databases

    PRIDEi Q9WVK5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000070080 ; ENSMUSP00000066515 ; ENSMUSG00000056124 .
    GeneIDi 56386.
    KEGGi mmu:56386.
    UCSCi uc008eeu.2. mouse.

    Organism-specific databases

    CTDi 9331.
    MGIi MGI:1928380. B4galt6.

    Phylogenomic databases

    eggNOGi NOG327897.
    GeneTreei ENSGT00750000117412.
    HOGENOMi HOG000231027.
    HOVERGENi HBG058334.
    InParanoidi Q9WVK5.
    KOi K07553.
    OMAi MYLPENF.
    OrthoDBi EOG7060R0.
    PhylomeDBi Q9WVK5.
    TreeFami TF312834.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    Reactomei REACT_198565. N-Glycan antennae elongation.
    REACT_198578. Keratan sulfate biosynthesis.

    Miscellaneous databases

    NextBioi 312476.
    PROi Q9WVK5.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9WVK5.
    Bgeei Q9WVK5.
    Genevestigatori Q9WVK5.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR003859. Galactosyl_T.
    IPR027791. Galactosyl_T_C.
    IPR027995. Galactosyl_T_N.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    PANTHERi PTHR19300. PTHR19300. 1 hit.
    Pfami PF02709. Glyco_transf_7C. 1 hit.
    PF13733. Glyco_transf_7N. 1 hit.
    [Graphical view ]
    PRINTSi PR02050. B14GALTRFASE.
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning and expression of mouse lactosylceramide synthase."
      Takizawa M., Nomura T., Wakisaka E., Aoki J., Arai H., Inoue K., Matsuo N.
      Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6J.
      Tissue: Brain.
    2. "Murine beta-1,4-galactosyltransferase family members."
      Lo N.-W., Shaper N.L., Shaper J.H.
      Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Mammary gland.

    Entry informationi

    Entry nameiB4GT6_MOUSE
    AccessioniPrimary (citable) accession number: Q9WVK5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 19, 2002
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3