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Protein

Beta-1,4-galactosyltransferase 6

Gene

B4galt6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Required for the biosynthesis of glycosphingolipids.By similarity

Catalytic activityi

UDP-alpha-D-galactose + beta-D-glucosyl-(1<->1)-ceramide = UDP + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.

Cofactori

Mn2+By similarity, Mg2+By similarity, Ca2+By similarity

Enzyme regulationi

Inhibited by EDTA.By similarity

Pathway: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi230 – 2301ManganeseBy similarity
Binding sitei258 – 2581UDP-alpha-D-galactoseBy similarity
Binding sitei290 – 2901UDP-alpha-D-galactoseBy similarity
Metal bindingi323 – 3231Manganese; via tele nitrogenBy similarity
Binding sitei334 – 3341N-acetyl-D-glucosamineBy similarity

GO - Molecular functioni

GO - Biological processi

  • protein glycosylation Source: UniProtKB-UniPathway
  • sphingolipid biosynthetic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Calcium, Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_308816. N-Glycan antennae elongation.
REACT_346228. Keratan sulfate biosynthesis.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-galactosyltransferase 6 (EC:2.4.1.-)
Short name:
Beta-1,4-GalTase 6
Short name:
Beta4Gal-T6
Short name:
b4Gal-T6
Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 6
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 6
Including the following 1 domains:
Glucosylceramide beta-1,4-galactosyltransferase (EC:2.4.1.274)
Alternative name(s):
Lactosylceramide synthase
Short name:
LacCer synthase
UDP-Gal:glucosylceramide beta-1,4-galactosyltransferase
Gene namesi
Name:B4galt6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1928380. B4galt6.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1515CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei16 – 3520Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini36 – 382347LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 382382Beta-1,4-galactosyltransferase 6PRO_0000080548Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi71 – 711N-linked (GlcNAc...)Sequence Analysis
Glycosylationi75 – 751N-linked (GlcNAc...)Sequence Analysis
Glycosylationi83 – 831N-linked (GlcNAc...)Sequence Analysis
Glycosylationi84 – 841N-linked (GlcNAc...)Sequence Analysis
Glycosylationi99 – 991N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi108 ↔ 152By similarity
Glycosylationi122 – 1221N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi223 ↔ 242By similarity
Glycosylationi307 – 3071N-linked (GlcNAc...)Sequence Analysis
Glycosylationi367 – 3671N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ9WVK5.

PTM databases

PhosphoSiteiQ9WVK5.

Expressioni

Gene expression databases

BgeeiQ9WVK5.
ExpressionAtlasiQ9WVK5. baseline and differential.
GenevisibleiQ9WVK5. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000066515.

Structurei

3D structure databases

ProteinModelPortaliQ9WVK5.
SMRiQ9WVK5. Positions 108-372.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni163 – 1675UDP-alpha-D-galactose bindingBy similarity
Regioni202 – 2043UDP-alpha-D-galactose bindingBy similarity
Regioni229 – 2302UDP-alpha-D-galactose bindingBy similarity
Regioni292 – 2954N-acetyl-D-glucosamine bindingBy similarity
Regioni323 – 3242UDP-alpha-D-galactose bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 7 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG327897.
GeneTreeiENSGT00760000119140.
HOGENOMiHOG000231027.
HOVERGENiHBG058334.
InParanoidiQ9WVK5.
KOiK07553.
OMAiMYLPENF.
OrthoDBiEOG7060R0.
PhylomeDBiQ9WVK5.
TreeFamiTF312834.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9WVK5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSALKRMMRV SNRSLIAFIF FFSLSTSCLY FIYVAPGIAN TYLFMVQARG
60 70 80 90 100
IMLRENVKTI GHMIRLYTNK NTTLNGTDYP EGNNTSDYLV QTTTYLPQNF
110 120 130 140 150
TYLPHLPCPE KLPYMRGFLS VNVSEISFDE VHQLFSKDSE IGPGGHWRPK
160 170 180 190 200
DCKPRWKVAV LIPFRNRHEH LPIFFLHLIP MLQKQRLEFA FYVIEQTGTQ
210 220 230 240 250
PFNRAMLFNV GFKEAMKDRA WDCVIFHDVD HLPENDRNYY GCGEMPRHFA
260 270 280 290 300
AKLDKYMYIL PYKEFFGGVS GLTVEQFRKI NGFPNAFWGW GGEDDDLWNR
310 320 330 340 350
VHYAGYNVTR PEGDLGKYIS IPHHHRGEVQ FLGRYKLLRY SKERQYIDGL
360 370 380
NNLLYTPKIL VDRLYTNISV NLMPELAPIE DY
Length:382
Mass (Da):44,759
Last modified:November 1, 1999 - v1
Checksum:i0223621127896358
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF097158 mRNA. Translation: AAD41694.1.
AF142674 mRNA. Translation: AAF22224.1.
BC011149 mRNA. Translation: AAH11149.1.
CCDSiCCDS29086.1.
RefSeqiNP_062711.1. NM_019737.2.
UniGeneiMm.393827.

Genome annotation databases

EnsembliENSMUST00000070080; ENSMUSP00000066515; ENSMUSG00000056124.
GeneIDi56386.
KEGGimmu:56386.
UCSCiuc008eeu.2. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

b4GalT6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF097158 mRNA. Translation: AAD41694.1.
AF142674 mRNA. Translation: AAF22224.1.
BC011149 mRNA. Translation: AAH11149.1.
CCDSiCCDS29086.1.
RefSeqiNP_062711.1. NM_019737.2.
UniGeneiMm.393827.

3D structure databases

ProteinModelPortaliQ9WVK5.
SMRiQ9WVK5. Positions 108-372.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000066515.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

PTM databases

PhosphoSiteiQ9WVK5.

Proteomic databases

PRIDEiQ9WVK5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000070080; ENSMUSP00000066515; ENSMUSG00000056124.
GeneIDi56386.
KEGGimmu:56386.
UCSCiuc008eeu.2. mouse.

Organism-specific databases

CTDi9331.
MGIiMGI:1928380. B4galt6.

Phylogenomic databases

eggNOGiNOG327897.
GeneTreeiENSGT00760000119140.
HOGENOMiHOG000231027.
HOVERGENiHBG058334.
InParanoidiQ9WVK5.
KOiK07553.
OMAiMYLPENF.
OrthoDBiEOG7060R0.
PhylomeDBiQ9WVK5.
TreeFamiTF312834.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiREACT_308816. N-Glycan antennae elongation.
REACT_346228. Keratan sulfate biosynthesis.

Miscellaneous databases

NextBioi312476.
PROiQ9WVK5.
SOURCEiSearch...

Gene expression databases

BgeeiQ9WVK5.
ExpressionAtlasiQ9WVK5. baseline and differential.
GenevisibleiQ9WVK5. MM.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning and expression of mouse lactosylceramide synthase."
    Takizawa M., Nomura T., Wakisaka E., Aoki J., Arai H., Inoue K., Matsuo N.
    Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  2. "Murine beta-1,4-galactosyltransferase family members."
    Lo N.-W., Shaper N.L., Shaper J.H.
    Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary gland.

Entry informationi

Entry nameiB4GT6_MOUSE
AccessioniPrimary (citable) accession number: Q9WVK5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: November 1, 1999
Last modified: June 24, 2015
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.