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Protein

EH domain-containing protein 1

Gene

Ehd1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Plays a role in myoblast fusion. Recruited to endosomal membranes upon nerve growth factor stimulation, indirectly regulates neurite outgrowth.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei220 – 2201ATPBy similarity
Binding sitei258 – 2581ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi65 – 728ATPBy similarity
Calcium bindingi489 – 50012PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • cellular response to nerve growth factor stimulus Source: UniProtKB
  • cholesterol homeostasis Source: BHF-UCL
  • endocytic recycling Source: UniProtKB
  • endocytosis Source: MGI
  • endosomal transport Source: MGI
  • intracellular protein transport Source: UniProtKB
  • low-density lipoprotein particle clearance Source: BHF-UCL
  • neuron projection development Source: UniProtKB
  • positive regulation of cholesterol storage Source: BHF-UCL
  • positive regulation of endocytic recycling Source: UniProtKB
  • positive regulation of myoblast fusion Source: UniProtKB
  • protein homooligomerization Source: MGI
Complete GO annotation...

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_280010. Factors involved in megakaryocyte development and platelet production.

Names & Taxonomyi

Protein namesi
Recommended name:
EH domain-containing protein 1
Alternative name(s):
PAST homolog 1
Short name:
mPAST1
Gene namesi
Name:Ehd1
Synonyms:Past1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1341878. Ehd1.

Subcellular locationi

GO - Cellular componenti

  • early endosome Source: MGI
  • early endosome membrane Source: UniProtKB
  • endocytic vesicle Source: MGI
  • endosome Source: MGI
  • endosome membrane Source: UniProtKB
  • extracellular exosome Source: MGI
  • lipid particle Source: BHF-UCL
  • membrane Source: MGI
  • myelin sheath Source: UniProtKB
  • perinuclear region of cytoplasm Source: MGI
  • plasma membrane Source: UniProtKB
  • platelet dense tubular network membrane Source: BHF-UCL
  • recycling endosome membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi65 – 651G → R: Abolishes interaction with PACSIN2. 1 Publication
Mutagenesisi485 – 4851W → A: Abolishes interaction with PACSIN2. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 534534EH domain-containing protein 1PRO_0000146110Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei355 – 3551PhosphoserineBy similarity
Modified residuei456 – 4561PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiQ9WVK4.
PaxDbiQ9WVK4.
PRIDEiQ9WVK4.

PTM databases

PhosphoSiteiQ9WVK4.

Expressioni

Tissue specificityi

Highly expressed in testis. Also expressed in kidney, heart, intestine, and brain.

Developmental stagei

Expression is already noted at day 9.5 in the limb buds and pharyngeal arches and at day 10.5 in sclerotomes, at various elements of the branchial apparatus (mandible and hyoid), and in the occipital region. At day 15.5 expression peaks in cartilage, preceding hypertrophy and ossification, and at day 17.5 there is no expression in the bones.

Gene expression databases

BgeeiQ9WVK4.
CleanExiMM_EHD1.
ExpressionAtlasiQ9WVK4. baseline and differential.
GenevisibleiQ9WVK4. MM.

Interactioni

Subunit structurei

Interacts (via EH domain) with RAB11FIP2 (By similarity). Homooligomer, and heterooligomer with EHD2, EHD3 and EHD4. Interacts (via EH domain) with MICALL1 (via NPF1 motif); the interaction is direct and recruits EHD1 to membranes. Interacts with RAB35; the interaction is indirect through MICALL1 and recruits EHD1 to membranes. Interacts (via EH domain) with PACSIN2 (via NPF motifs); regulates localization to tubular recycling endosome membranes. Interacts with PACSIN1. Interacts with RAB8A. Interacts with FER1L5 (via second C2 domain). Interacts with MYOF. Interacts with ZFYVE20.By similarity3 Publications

Protein-protein interaction databases

BioGridi199408. 1 interaction.
DIPiDIP-59491N.
IntActiQ9WVK4. 4 interactions.
MINTiMINT-4998512.
STRINGi10090.ENSMUSP00000025684.

Structurei

3D structure databases

ProteinModelPortaliQ9WVK4.
SMRiQ9WVK4. Positions 1-534.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini55 – 286232Dynamin-type GAdd
BLAST
Domaini444 – 53289EHPROSITE-ProRule annotationAdd
BLAST
Domaini476 – 51136EF-handPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili198 – 22730Sequence AnalysisAdd
BLAST

Domaini

The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.1 Publication

Sequence similaritiesi

Contains 1 EF-hand domain.PROSITE-ProRule annotation
Contains 1 EH domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG136252.
GeneTreeiENSGT00760000118985.
HOVERGENiHBG018183.
InParanoidiQ9WVK4.
KOiK12483.
OMAiQAYIISS.
OrthoDBiEOG757CX9.
PhylomeDBiQ9WVK4.
TreeFamiTF314429.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR029947. EHD1.
IPR000261. EPS15_homology.
IPR030381. G_DYNAMIN_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11216:SF60. PTHR11216:SF60. 1 hit.
SMARTiSM00027. EH. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50031. EH. 1 hit.
PS51718. G_DYNAMIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WVK4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSWVSKDAR RKKEPELFQT VAEGLRQLYA QKLLPLEEHY RFHEFHSPAL
60 70 80 90 100
EDADFDNKPM VLLVGQYSTG KTTFIRHLIE QDFPGMRIGP EPTTDSFIAV
110 120 130 140 150
MHGPTEGVVP GNALVVDPRR PFRKLNAFGN AFLNRFMCAQ LPNPVLDSIS
160 170 180 190 200
IIDTPGILSG EKQRISRGYD FAAVLEWFAE RVDRIILLFD AHKLDISDEF
210 220 230 240 250
SEVIKALKNH EDKIRVVLNK ADQIETQQLM RVYGALMWSL GKIINTPEVV
260 270 280 290 300
RVYIGSFWSH PLLIPDNRKL FEAEEQDLFK DIQSLPRNAA LRKLNDLIKR
310 320 330 340 350
ARLAKVHAYI ISSLKKEMPN VFGKESKKKE LVNNLGEIYQ KIEREHQISS
360 370 380 390 400
GDFPSLRKMQ ELLQTQDFSK FQALKPKLLD TVDDMLANDI ARLMVMVRQE
410 420 430 440 450
ESLMPSQAVK GGAFDGTMNG PFGHGYGEGA GEGIDDVEWV VGKDKPTYDE
460 470 480 490 500
IFYTLSPVNG KITGANAKKE MVKSKLPNTV LGKIWKLADV DKDGLLDDEE
510 520 530
FALANHLIKV KLEGHELPAD LPPHLIPPSK RRHE
Length:534
Mass (Da):60,603
Last modified:November 1, 1999 - v1
Checksum:i35502055BC6164F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF099186 mRNA. Translation: AAD45423.1.
AF173156 mRNA. Translation: AAF24223.1.
AK012896 mRNA. Translation: BAB28540.1.
CCDSiCCDS29501.1.
RefSeqiNP_034249.1. NM_010119.5.
UniGeneiMm.30169.

Genome annotation databases

EnsembliENSMUST00000025684; ENSMUSP00000025684; ENSMUSG00000024772.
GeneIDi13660.
KEGGimmu:13660.
UCSCiuc008ghz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF099186 mRNA. Translation: AAD45423.1.
AF173156 mRNA. Translation: AAF24223.1.
AK012896 mRNA. Translation: BAB28540.1.
CCDSiCCDS29501.1.
RefSeqiNP_034249.1. NM_010119.5.
UniGeneiMm.30169.

3D structure databases

ProteinModelPortaliQ9WVK4.
SMRiQ9WVK4. Positions 1-534.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199408. 1 interaction.
DIPiDIP-59491N.
IntActiQ9WVK4. 4 interactions.
MINTiMINT-4998512.
STRINGi10090.ENSMUSP00000025684.

PTM databases

PhosphoSiteiQ9WVK4.

Proteomic databases

MaxQBiQ9WVK4.
PaxDbiQ9WVK4.
PRIDEiQ9WVK4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025684; ENSMUSP00000025684; ENSMUSG00000024772.
GeneIDi13660.
KEGGimmu:13660.
UCSCiuc008ghz.1. mouse.

Organism-specific databases

CTDi10938.
MGIiMGI:1341878. Ehd1.

Phylogenomic databases

eggNOGiNOG136252.
GeneTreeiENSGT00760000118985.
HOVERGENiHBG018183.
InParanoidiQ9WVK4.
KOiK12483.
OMAiQAYIISS.
OrthoDBiEOG757CX9.
PhylomeDBiQ9WVK4.
TreeFamiTF314429.

Enzyme and pathway databases

ReactomeiREACT_280010. Factors involved in megakaryocyte development and platelet production.

Miscellaneous databases

ChiTaRSiEhd1. mouse.
NextBioi284400.
PROiQ9WVK4.
SOURCEiSearch...

Gene expression databases

BgeeiQ9WVK4.
CleanExiMM_EHD1.
ExpressionAtlasiQ9WVK4. baseline and differential.
GenevisibleiQ9WVK4. MM.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR029947. EHD1.
IPR000261. EPS15_homology.
IPR030381. G_DYNAMIN_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11216:SF60. PTHR11216:SF60. 1 hit.
SMARTiSM00027. EH. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS50031. EH. 1 hit.
PS51718. G_DYNAMIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "EHD1 -- an EH-domain-containing protein with a specific expression pattern."
    Mintz L., Galperin E., Pasmanik-Chor M., Tulzinsky S., Bromberg Y., Kozak C.A., Joyner A., Fein A., Horowitz M.
    Genomics 59:66-76(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ICR.
  2. Plomann M., Behrendt D., Ritter B., Modregger J., Halback A., Paulsson M.
    Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo.
  4. "EHD proteins associate with syndapin I and II and such interactions play a crucial role in endosomal recycling."
    Braun A., Pinyol R., Dahlhaus R., Koch D., Fonarev P., Grant B.D., Kessels M.M., Qualmann B.
    Mol. Biol. Cell 16:3642-3658(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DOMAIN, MUTAGENESIS OF GLY-65 AND TRP-485, INTERACTION WITH PACSIN1 AND PACSIN2.
  5. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
    Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
    Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "Endocytic recycling proteins EHD1 and EHD2 interact with fer-1-like-5 (Fer1L5) and mediate myoblast fusion."
    Posey A.D. Jr., Pytel P., Gardikiotes K., Demonbreun A.R., Rainey M., George M., Band H., McNally E.M.
    J. Biol. Chem. 286:7379-7388(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH FER1L5 AND MYOF, SUBCELLULAR LOCATION.
  7. "Rab35 establishes the EHD1-association site by coordinating two distinct effectors during neurite outgrowth."
    Kobayashi H., Fukuda M.
    J. Cell Sci. 126:2424-2435(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MICALL1 AND RAB35.

Entry informationi

Entry nameiEHD1_MOUSE
AccessioniPrimary (citable) accession number: Q9WVK4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: November 1, 1999
Last modified: July 22, 2015
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.