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Q9WVK4

- EHD1_MOUSE

UniProt

Q9WVK4 - EHD1_MOUSE

Protein

EH domain-containing protein 1

Gene

Ehd1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Acts in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Plays a role in myoblast fusion. Recruited to endosomal membranes upon nerve growth factor stimulation, indirectly regulates neurite outgrowth.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei220 – 2201ATPBy similarity
    Binding sitei258 – 2581ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi65 – 728ATPBy similarity
    Calcium bindingi489 – 50012PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. calcium ion binding Source: InterPro
    3. GTPase activity Source: InterPro
    4. GTP binding Source: InterPro
    5. protein binding Source: MGI

    GO - Biological processi

    1. cellular response to nerve growth factor stimulus Source: UniProtKB
    2. cholesterol homeostasis Source: BHF-UCL
    3. endocytic recycling Source: UniProtKB
    4. endocytosis Source: MGI
    5. endosomal transport Source: MGI
    6. intracellular protein transport Source: UniProtKB
    7. low-density lipoprotein particle clearance Source: BHF-UCL
    8. neuron projection development Source: UniProtKB
    9. positive regulation of cholesterol storage Source: BHF-UCL
    10. positive regulation of endocytic recycling Source: UniProtKB
    11. positive regulation of myoblast fusion Source: UniProtKB
    12. protein homooligomerization Source: Ensembl

    Keywords - Ligandi

    ATP-binding, Calcium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_198649. Factors involved in megakaryocyte development and platelet production.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    EH domain-containing protein 1
    Alternative name(s):
    PAST homolog 1
    Short name:
    mPAST1
    Gene namesi
    Name:Ehd1
    Synonyms:Past1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:1341878. Ehd1.

    Subcellular locationi

    Recycling endosome membrane By similarity; Peripheral membrane protein By similarity. Early endosome membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein; Cytoplasmic side
    Note: Preferentially associates with tubular recycling endosomes. Colocalizes with FER1L5 at plasma membrane in myoblasts and myotubes.

    GO - Cellular componenti

    1. early endosome Source: MGI
    2. early endosome membrane Source: UniProtKB
    3. endocytic vesicle Source: MGI
    4. endosome Source: MGI
    5. endosome membrane Source: UniProtKB
    6. lipid particle Source: BHF-UCL
    7. perinuclear region of cytoplasm Source: MGI
    8. plasma membrane Source: UniProtKB
    9. platelet dense tubular network membrane Source: BHF-UCL
    10. recycling endosome membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Endosome, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi65 – 651G → R: Abolishes interaction with PACSIN2. 1 Publication
    Mutagenesisi485 – 4851W → A: Abolishes interaction with PACSIN2. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 534534EH domain-containing protein 1PRO_0000146110Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei456 – 4561PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ9WVK4.
    PaxDbiQ9WVK4.
    PRIDEiQ9WVK4.

    PTM databases

    PhosphoSiteiQ9WVK4.

    Expressioni

    Tissue specificityi

    Highly expressed in testis. Also expressed in kidney, heart, intestine, and brain.

    Developmental stagei

    Expression is already noted at day 9.5 in the limb buds and pharyngeal arches and at day 10.5 in sclerotomes, at various elements of the branchial apparatus (mandible and hyoid), and in the occipital region. At day 15.5 expression peaks in cartilage, preceding hypertrophy and ossification, and at day 17.5 there is no expression in the bones.

    Gene expression databases

    ArrayExpressiQ9WVK4.
    BgeeiQ9WVK4.
    CleanExiMM_EHD1.
    GenevestigatoriQ9WVK4.

    Interactioni

    Subunit structurei

    Interacts (via EH domain) with RAB11FIP2 By similarity. Homooligomer, and heterooligomer with EHD2, EHD3 and EHD4. Interacts (via EH domain) with MICALL1 (via NPF1 motif); the interaction is direct and recruits EHD1 to membranes. Interacts with RAB35; the interaction is indirect through MICALL1 and recruits EHD1 to membranes. Interacts (via EH domain) with PACSIN2 (via NPF motifs); regulates localization to tubular recycling endosome membranes. Interacts with PACSIN1. Interacts with RAB8A. Interacts with FER1L5 (via second C2 domain). Interacts with MYOF. Interacts with ZFYVE20.By similarity3 Publications

    Protein-protein interaction databases

    BioGridi199408. 1 interaction.
    DIPiDIP-59491N.
    IntActiQ9WVK4. 3 interactions.
    MINTiMINT-4998512.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9WVK4.
    SMRiQ9WVK4. Positions 1-534.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini55 – 286232Dynamin-type GAdd
    BLAST
    Domaini444 – 53289EHPROSITE-ProRule annotationAdd
    BLAST
    Domaini476 – 51136EF-handPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili198 – 22730Sequence AnalysisAdd
    BLAST

    Domaini

    The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.1 Publication

    Sequence similaritiesi

    Contains 1 EF-hand domain.PROSITE-ProRule annotation
    Contains 1 EH domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG136252.
    GeneTreeiENSGT00700000104190.
    HOVERGENiHBG018183.
    InParanoidiQ9WVK4.
    KOiK12483.
    OMAiQAYIISS.
    OrthoDBiEOG757CX9.
    PhylomeDBiQ9WVK4.
    TreeFamiTF314429.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR001401. Dynamin_GTPase.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR000261. EPS15_homology.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00350. Dynamin_N. 1 hit.
    [Graphical view]
    SMARTiSM00027. EH. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 1 hit.
    PS50031. EH. 1 hit.
    PS51718. G_DYNAMIN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9WVK4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFSWVSKDAR RKKEPELFQT VAEGLRQLYA QKLLPLEEHY RFHEFHSPAL    50
    EDADFDNKPM VLLVGQYSTG KTTFIRHLIE QDFPGMRIGP EPTTDSFIAV 100
    MHGPTEGVVP GNALVVDPRR PFRKLNAFGN AFLNRFMCAQ LPNPVLDSIS 150
    IIDTPGILSG EKQRISRGYD FAAVLEWFAE RVDRIILLFD AHKLDISDEF 200
    SEVIKALKNH EDKIRVVLNK ADQIETQQLM RVYGALMWSL GKIINTPEVV 250
    RVYIGSFWSH PLLIPDNRKL FEAEEQDLFK DIQSLPRNAA LRKLNDLIKR 300
    ARLAKVHAYI ISSLKKEMPN VFGKESKKKE LVNNLGEIYQ KIEREHQISS 350
    GDFPSLRKMQ ELLQTQDFSK FQALKPKLLD TVDDMLANDI ARLMVMVRQE 400
    ESLMPSQAVK GGAFDGTMNG PFGHGYGEGA GEGIDDVEWV VGKDKPTYDE 450
    IFYTLSPVNG KITGANAKKE MVKSKLPNTV LGKIWKLADV DKDGLLDDEE 500
    FALANHLIKV KLEGHELPAD LPPHLIPPSK RRHE 534
    Length:534
    Mass (Da):60,603
    Last modified:November 1, 1999 - v1
    Checksum:i35502055BC6164F7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF099186 mRNA. Translation: AAD45423.1.
    AF173156 mRNA. Translation: AAF24223.1.
    AK012896 mRNA. Translation: BAB28540.1.
    CCDSiCCDS29501.1.
    RefSeqiNP_034249.1. NM_010119.5.
    UniGeneiMm.30169.

    Genome annotation databases

    EnsembliENSMUST00000025684; ENSMUSP00000025684; ENSMUSG00000024772.
    GeneIDi13660.
    KEGGimmu:13660.
    UCSCiuc008ghz.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF099186 mRNA. Translation: AAD45423.1 .
    AF173156 mRNA. Translation: AAF24223.1 .
    AK012896 mRNA. Translation: BAB28540.1 .
    CCDSi CCDS29501.1.
    RefSeqi NP_034249.1. NM_010119.5.
    UniGenei Mm.30169.

    3D structure databases

    ProteinModelPortali Q9WVK4.
    SMRi Q9WVK4. Positions 1-534.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199408. 1 interaction.
    DIPi DIP-59491N.
    IntActi Q9WVK4. 3 interactions.
    MINTi MINT-4998512.

    PTM databases

    PhosphoSitei Q9WVK4.

    Proteomic databases

    MaxQBi Q9WVK4.
    PaxDbi Q9WVK4.
    PRIDEi Q9WVK4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000025684 ; ENSMUSP00000025684 ; ENSMUSG00000024772 .
    GeneIDi 13660.
    KEGGi mmu:13660.
    UCSCi uc008ghz.1. mouse.

    Organism-specific databases

    CTDi 10938.
    MGIi MGI:1341878. Ehd1.

    Phylogenomic databases

    eggNOGi NOG136252.
    GeneTreei ENSGT00700000104190.
    HOVERGENi HBG018183.
    InParanoidi Q9WVK4.
    KOi K12483.
    OMAi QAYIISS.
    OrthoDBi EOG757CX9.
    PhylomeDBi Q9WVK4.
    TreeFami TF314429.

    Enzyme and pathway databases

    Reactomei REACT_198649. Factors involved in megakaryocyte development and platelet production.

    Miscellaneous databases

    NextBioi 284400.
    PROi Q9WVK4.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9WVK4.
    Bgeei Q9WVK4.
    CleanExi MM_EHD1.
    Genevestigatori Q9WVK4.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR001401. Dynamin_GTPase.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR000261. EPS15_homology.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00350. Dynamin_N. 1 hit.
    [Graphical view ]
    SMARTi SM00027. EH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 1 hit.
    PS50031. EH. 1 hit.
    PS51718. G_DYNAMIN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "EHD1 -- an EH-domain-containing protein with a specific expression pattern."
      Mintz L., Galperin E., Pasmanik-Chor M., Tulzinsky S., Bromberg Y., Kozak C.A., Joyner A., Fein A., Horowitz M.
      Genomics 59:66-76(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: ICR.
    2. Plomann M., Behrendt D., Ritter B., Modregger J., Halback A., Paulsson M.
      Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6J.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Embryo.
    4. "EHD proteins associate with syndapin I and II and such interactions play a crucial role in endosomal recycling."
      Braun A., Pinyol R., Dahlhaus R., Koch D., Fonarev P., Grant B.D., Kessels M.M., Qualmann B.
      Mol. Biol. Cell 16:3642-3658(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, DOMAIN, MUTAGENESIS OF GLY-65 AND TRP-485, INTERACTION WITH PACSIN1 AND PACSIN2.
    5. "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells."
      Rush J., Moritz A., Lee K.A., Guo A., Goss V.L., Spek E.J., Zhang H., Zha X.-M., Polakiewicz R.D., Comb M.J.
      Nat. Biotechnol. 23:94-101(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    6. "Endocytic recycling proteins EHD1 and EHD2 interact with fer-1-like-5 (Fer1L5) and mediate myoblast fusion."
      Posey A.D. Jr., Pytel P., Gardikiotes K., Demonbreun A.R., Rainey M., George M., Band H., McNally E.M.
      J. Biol. Chem. 286:7379-7388(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH FER1L5 AND MYOF, SUBCELLULAR LOCATION.
    7. "Rab35 establishes the EHD1-association site by coordinating two distinct effectors during neurite outgrowth."
      Kobayashi H., Fukuda M.
      J. Cell Sci. 126:2424-2435(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MICALL1 AND RAB35.

    Entry informationi

    Entry nameiEHD1_MOUSE
    AccessioniPrimary (citable) accession number: Q9WVK4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3