Reviewed, UniProtKB/Swiss-Prot Q9WVK3 (PECR_RAT)

Names and origin

Protein names

Recommended name:
    Peroxisomal trans-2-enoyl-CoA reductase
    EC=1.3.1.38
Alternative name(s):
    RLF98
    Peroxisomal 2,4-dienoyl-CoA reductase
    PX-2,4-DCR1

Gene names

Name:

Pecr

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Participates in chain elongation of fatty acids. Has no 2,4-dienoyl-CoA reductase activity By similarity.
Catalytic activity	Acyl-CoA + NADP+ = trans-2,3-dehydroacyl-CoA + NADPH.
Pathway	Lipid metabolism; fatty acid biosynthesis.
Subunit structure	Interacts with PEX5, probably required to target it into peroxisomes By similarity.
Subcellular location	Peroxisome By similarity.
Tissue specificity	Highly expressed in liver and kidney. Weakly expressed in other tissues. Ref.1
Induction	Up-regulated by fasting. Ref.1
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Peroxisome
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from sequence or structural similarity. Source: UniProtKB peroxisome Inferred from direct assay. Source: HGNC
Molecular function	binding Inferred from electronic annotation. Source: InterPro trans-2-enoyl-CoA reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	Peroxisomal trans-2-enoyl-CoA reductase		PRO_0000054743
Regions
	Nucleotide binding	23 – 47	25	NADP By similarity
	Motif	301 – 303	3	Microbody targeting signal By similarity
Sites
	Active site	179	1	Proton acceptor By similarity
Amino acid modifications
	Modified residue	179	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9WVK3-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: F137C5517D655D7B	FASTA	303	32,433
	10 20 30 40 50 60 MGSWKSGQSY LAAGLLQNQV AVVTGGATGI GKAISRELLH LGCNVVIASR KLDRLTAAVD 70 80 90 100 110 120 ELRASQPPSS STQVTAIQCN IRKEEEVNNL VKSTLAKYGK INFLVNNAGG QFMAPAEDIT 130 140 150 160 170 180 AKGWQAVIET NLTGTFYMCK AVYNSWMKDH GGSIVNIIVL LNNGFPTAAH SGAARAGVYN 190 200 210 220 230 240 LTKTMALTWA SSGVRINCVA PGTIYSQTAV DNYGELGQTM FEMAFENIPA KRVGLPEEIS 250 260 270 280 290 300 PLVCFLLSPA ASFITGQLIN VDGGQALYTR NFTIPDHDNW PVGAGDSSFI KKVKESLKKQ ARL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of a new fasting-inducible short-chain dehydrogenase/reductase from rat liver." Zhang J., Underwood L.E. Biochim. Biophys. Acta 1435:184-190(1999) [PubMed: 10561551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION. Strain: Sprague-Dawley. Tissue: Liver.
[2]	"Mitochondria and peroxisomes contain distinct isoforms of 2,4-dienoyl CoA reductase that interact with an Hsp70 member: isolation and characterisation of a cDNA encoding rat peroxisomal 2,4-dienoyl CoA reductase." Naylor D.J., Koivurantac K.T., Stines A.P., Hiltunen J.K., Hoogenraad N.J., Hoj P.B. Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pituitary.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF099742 mRNA. Translation: AAD38447.1. AF021854 mRNA. Translation: AAF14047.1. BC060546 mRNA. Translation: AAH60546.1.
IPI	IPI00326195.
RefSeq	NP_579833.1.
UniGene	Rn.163081
3D structure databases
HSSP	HSSP built from PDB template 1AE1 based on UniProtKB P50162.
SMR	Q9WVK3. Positions 7-303.
ModBase	Search...
Proteomic databases
PRIDE	Q9WVK3.
Genome annotation databases
Ensembl	ENSRNOT00000021512; ENSRNOP00000021512; ENSRNOG00000015809; Rattus norvegicus. [Genome view]
GeneID	113956.
KEGG	rno:113956.
Organism-specific databases
CTD	113956.
RGD	70925. Pecr.
Phylogenomic databases
HOVERGEN	Q9WVK3.
Enzyme and pathway databases
BRENDA	1.3.1.38. 248.
Gene expression databases
ArrayExpress	Q9WVK3.
Genevestigator	Q9WVK3.
GermOnline	ENSRNOG00000015809. Rattus norvegicus.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH.
PROSITE	PS00061. ADH_SHORT. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	618083.

Entry information

Entry name

PECR_RAT

Accession

Primary (citable) accession number: Q9WVK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	November 1, 1999
Last modified:	October 13, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)