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Protein

Synaptojanin-2-binding protein

Gene

Synj2bp

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates endocytosis of activin type 2 receptor kinases through the Ral/RALBP1-dependent pathway and may be involved in suppression of activin-induced signal transduction.By similarity

GO - Molecular functioni

  • protein C-terminus binding Source: RGD

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptojanin-2-binding protein
Alternative name(s):
Mitochondrial outer membrane protein 25
NPW16
Gene namesi
Name:Synj2bp
Synonyms:Omp25
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 6

Organism-specific databases

RGDi69400. Synj2bp.

Subcellular locationi

  • Mitochondrion outer membrane 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 117117CytoplasmicSequence analysisAdd
BLAST
Transmembranei118 – 13821HelicalSequence analysisAdd
BLAST
Topological domaini139 – 1457Mitochondrial intermembraneSequence analysis

GO - Cellular componenti

  • cell surface Source: Ensembl
  • integral component of mitochondrial outer membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 145145Synaptojanin-2-binding proteinPRO_0000072384Add
BLAST

Proteomic databases

PaxDbiQ9WVJ4.
PRIDEiQ9WVJ4.

PTM databases

iPTMnetiQ9WVJ4.
PhosphoSiteiQ9WVJ4.

Expressioni

Tissue specificityi

Widely expressed.

Gene expression databases

GenevisibleiQ9WVJ4. RN.

Interactioni

Subunit structurei

Binds (via the PDZ domain) to isoform 2A of SYNJ2 (via the unique motif in the C-terminus) (PubMed:10357812). Interacts (via C-terminus) with RALBP1. Interacts (via PDZ domain) with ACVR2A (via C-terminus) and ACVR2B (via C-terminus). Forms a ternary complex with ACVR2A and RALBP1 (By similarity). Interacts with MAPK12 (PubMed:15878399). Interacts with DLL1; enhances DLL1 protein stability, and promotes notch signaling in endothelial cells (By similarity).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Synj2O55207-39EBI-7007454,EBI-7007476

GO - Molecular functioni

  • protein C-terminus binding Source: RGD

Protein-protein interaction databases

BioGridi249115. 1 interaction.
IntActiQ9WVJ4. 1 interaction.
MINTiMINT-1216281.
STRINGi10116.ENSRNOP00000009119.

Structurei

3D structure databases

ProteinModelPortaliQ9WVJ4.
SMRiQ9WVJ4. Positions 1-107.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 10088PDZPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410KDNK. Eukaryota.
ENOG4111VAQ. LUCA.
GeneTreeiENSGT00830000128402.
HOGENOMiHOG000146436.
HOVERGENiHBG061461.
InParanoidiQ9WVJ4.
OMAiQNGPLGH.
OrthoDBiEOG7JQBQV.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR001478. PDZ.
[Graphical view]
PfamiPF00595. PDZ. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
PROSITEiPS50106. PDZ. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WVJ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNGRVDYLVS EEEINLTRGP SGLGFNIVGG TDQQYVSNDS GIYVSRIKED
60 70 80 90 100
GAAARDGRLQ EGDKILSVNG QDLKNLLHQD AVDLFRNAGY AVSLRVQHRL
110 120 130 140
PVQNGPIVHR GDGEPSGVPV AVVLLPVFAL TLVAVWAFVR YRKQL
Length:145
Mass (Da):15,825
Last modified:November 25, 2008 - v2
Checksum:i846CD232A73FE266
GO

Sequence cautioni

The sequence AAD39893.1 differs from that shown.Chimeric cDNA.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF107295 mRNA. Translation: AAD39893.1. Sequence problems.
AF260258 mRNA. Translation: AAF70306.1.
BC126067 mRNA. Translation: AAI26068.2.
RefSeqiNP_072121.2. NM_022599.2.
UniGeneiRn.6258.

Genome annotation databases

EnsembliENSRNOT00000009122; ENSRNOP00000009119; ENSRNOG00000006399.
GeneIDi64531.
KEGGirno:64531.
UCSCiRGD:69400. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF107295 mRNA. Translation: AAD39893.1. Sequence problems.
AF260258 mRNA. Translation: AAF70306.1.
BC126067 mRNA. Translation: AAI26068.2.
RefSeqiNP_072121.2. NM_022599.2.
UniGeneiRn.6258.

3D structure databases

ProteinModelPortaliQ9WVJ4.
SMRiQ9WVJ4. Positions 1-107.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249115. 1 interaction.
IntActiQ9WVJ4. 1 interaction.
MINTiMINT-1216281.
STRINGi10116.ENSRNOP00000009119.

PTM databases

iPTMnetiQ9WVJ4.
PhosphoSiteiQ9WVJ4.

Proteomic databases

PaxDbiQ9WVJ4.
PRIDEiQ9WVJ4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000009122; ENSRNOP00000009119; ENSRNOG00000006399.
GeneIDi64531.
KEGGirno:64531.
UCSCiRGD:69400. rat.

Organism-specific databases

CTDi55333.
RGDi69400. Synj2bp.

Phylogenomic databases

eggNOGiENOG410KDNK. Eukaryota.
ENOG4111VAQ. LUCA.
GeneTreeiENSGT00830000128402.
HOGENOMiHOG000146436.
HOVERGENiHBG061461.
InParanoidiQ9WVJ4.
OMAiQNGPLGH.
OrthoDBiEOG7JQBQV.

Miscellaneous databases

NextBioi613370.
PROiQ9WVJ4.

Gene expression databases

GenevisibleiQ9WVJ4. RN.

Family and domain databases

Gene3Di2.30.42.10. 1 hit.
InterProiIPR001478. PDZ.
[Graphical view]
PfamiPF00595. PDZ. 1 hit.
[Graphical view]
SMARTiSM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMiSSF50156. SSF50156. 1 hit.
PROSITEiPS50106. PDZ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Recruitment of an alternatively spliced form of synaptojanin 2 to mitochondria by the interaction with the PDZ domain of a mitochondrial outer membrane protein."
    Nemoto Y., De Camilli P.
    EMBO J. 18:2991-3006(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH SYNJ2, SUBCELLULAR LOCATION.
  2. "Production and characterization of monoclonal antibodies against NPW16: a novel PDZ protein."
    Jin W., Wang Y., Yang H., Liao B., Ju G.
    Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  4. "Outer membrane protein 25-a mitochondrial anchor and inhibitor of stress-activated protein kinase-3."
    Court N.W., Ingley E., Klinken S.P., Bogoyevitch M.A.
    Biochim. Biophys. Acta 1744:68-75(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MAPK12.

Entry informationi

Entry nameiSYJ2B_RAT
AccessioniPrimary (citable) accession number: Q9WVJ4
Secondary accession number(s): A0JN00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 25, 2008
Last modified: May 11, 2016
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.