Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Guanylate cyclase soluble subunit alpha-2

Gene

Gucy1a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has guanylyl cyclase on binding to the beta-1 subunit.

Catalytic activityi

GTP = 3',5'-cyclic GMP + diphosphate.

Enzyme regulationi

Activated by nitric oxide in the presence of magnesium or manganese ions.By similarity

GO - Molecular functioni

  • GTP binding Source: UniProtKB-KW
  • guanylate cyclase activity Source: RGD
  • heme binding Source: InterPro
  • protein heterodimerization activity Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

cGMP biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-392154. Nitric oxide stimulates guanylate cyclase.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanylate cyclase soluble subunit alpha-2 (EC:4.6.1.2)
Short name:
GCS-alpha-2
Gene namesi
Name:Gucy1a2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi621655. Gucy1a2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 730730Guanylate cyclase soluble subunit alpha-2PRO_0000074114Add
BLAST

Proteomic databases

PaxDbiQ9WVI4.
PRIDEiQ9WVI4.

PTM databases

iPTMnetiQ9WVI4.

Expressioni

Gene expression databases

GenevisibleiQ9WVI4. RN.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta chain.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Dlg4P310167EBI-7665590,EBI-375655
Dlg4Q621083EBI-7665590,EBI-300895From a different organism.

GO - Molecular functioni

  • protein heterodimerization activity Source: RGD

Protein-protein interaction databases

IntActiQ9WVI4. 5 interactions.
MINTiMINT-1782739.
STRINGi10116.ENSRNOP00000040361.

Structurei

3D structure databases

ProteinModelPortaliQ9WVI4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini519 – 646128Guanylate cyclasePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi50 – 7223Ala-richAdd
BLAST
Compositional biasi64 – 729Poly-Ala

Sequence similaritiesi

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation
Contains 1 guanylate cyclase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4171. Eukaryota.
COG2114. LUCA.
GeneTreeiENSGT00760000118959.
HOVERGENiHBG106603.
InParanoidiQ9WVI4.
KOiK12318.
OMAiVMEIKGQ.
OrthoDBiEOG7VX8Z6.
PhylomeDBiQ9WVI4.

Family and domain databases

Gene3Di3.30.70.1230. 1 hit.
InterProiIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR011644. Heme_NO-bd.
IPR011645. HNOB_dom_associated.
IPR024096. NO_sig/Golgi_transp_ligand-bd.
IPR029787. Nucleotide_cyclase.
[Graphical view]
PfamiPF00211. Guanylate_cyc. 1 hit.
PF07700. HNOB. 1 hit.
PF07701. HNOBA. 1 hit.
[Graphical view]
SMARTiSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMiSSF111126. SSF111126. 1 hit.
SSF55073. SSF55073. 1 hit.
PROSITEiPS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WVI4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRRKISSES FSSLGSDYLE TSPEEEGECP LSKLCWNGSR SPPGPPGSRA
60 70 80 90 100
AAMAATPVPA ASVAAAAAAV AAGSKRAQRR RRVNLDSLGE SISLLTAPSP
110 120 130 140 150
QTIHMTLKRT LQYYEHQVIG YRDAEKNFHN ISNRCSSADH SNKEEIEDVS
160 170 180 190 200
GILRCTANVL GLKFQEIQER FGEEFFKICF DENERVLRAV GSTLQDFFNG
210 220 230 240 250
FDALLEHIRT SFGKQATLES PSFLCKELPE GTLKLHYFHP HHTVGFAMLG
260 270 280 290 300
MIKAAGKRIY HLNVEVEQIE NEKFCSDGST PSNYSCLTFL IKECETTQIT
310 320 330 340 350
KNIPQGTSQI PTDLRISINT FCRTFPFHLM FDPNMVVLQL GEGLRKQLRC
360 370 380 390 400
DNHKVLKFED CFEIVSPKVN ATFDRVLLRL STPFVIRTKP EASGTDNEDK
410 420 430 440 450
VMEIKGQMIH VPESNAILFL GSPCVDKLDE LIGRGLHLSD IPIHDATRDV
460 470 480 490 500
ILVGEQAKAQ DGLKKRMDKL KATLEKTHQA LEEEKKKTVD LLYSIFPGDV
510 520 530 540 550
AQQLWQRQQV QARKFDDVTM LFSDIVGFTA ICAQCTPMQV ISMLNELYTR
560 570 580 590 600
FDHQCGFLDI YKVETIGDAY CVASGLHRKS LCHAKPIALM ALKMMELSEE
610 620 630 640 650
VLTPDGRPIQ MRIGIHSGSV LAGVVGVRMP RYCLFGNNVT LASKFESGSH
660 670 680 690 700
PRRINISPTT YQLLKREDSF TFIPRSREEL PDNFPKEIPG VCYFLELRTG
710 720 730
PKPPKPSLSS SRIKKVSYNI GTMFLRETSL
Length:730
Mass (Da):81,787
Last modified:March 1, 2001 - v2
Checksum:iB8D790BFF81FB8F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF109963 mRNA. Translation: AAD42949.2.
AB096080 mRNA. Translation: BAC24017.1.
RefSeqiNP_076446.1. NM_023956.1.
UniGeneiRn.209304.

Genome annotation databases

EnsembliENSRNOT00000046058; ENSRNOP00000040361; ENSRNOG00000029876.
GeneIDi66012.
KEGGirno:66012.
UCSCiRGD:621655. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF109963 mRNA. Translation: AAD42949.2.
AB096080 mRNA. Translation: BAC24017.1.
RefSeqiNP_076446.1. NM_023956.1.
UniGeneiRn.209304.

3D structure databases

ProteinModelPortaliQ9WVI4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9WVI4. 5 interactions.
MINTiMINT-1782739.
STRINGi10116.ENSRNOP00000040361.

PTM databases

iPTMnetiQ9WVI4.

Proteomic databases

PaxDbiQ9WVI4.
PRIDEiQ9WVI4.

Protocols and materials databases

DNASUi66012.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000046058; ENSRNOP00000040361; ENSRNOG00000029876.
GeneIDi66012.
KEGGirno:66012.
UCSCiRGD:621655. rat.

Organism-specific databases

CTDi2977.
RGDi621655. Gucy1a2.

Phylogenomic databases

eggNOGiKOG4171. Eukaryota.
COG2114. LUCA.
GeneTreeiENSGT00760000118959.
HOVERGENiHBG106603.
InParanoidiQ9WVI4.
KOiK12318.
OMAiVMEIKGQ.
OrthoDBiEOG7VX8Z6.
PhylomeDBiQ9WVI4.

Enzyme and pathway databases

ReactomeiR-RNO-392154. Nitric oxide stimulates guanylate cyclase.

Miscellaneous databases

PROiQ9WVI4.

Gene expression databases

GenevisibleiQ9WVI4. RN.

Family and domain databases

Gene3Di3.30.70.1230. 1 hit.
InterProiIPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR011644. Heme_NO-bd.
IPR011645. HNOB_dom_associated.
IPR024096. NO_sig/Golgi_transp_ligand-bd.
IPR029787. Nucleotide_cyclase.
[Graphical view]
PfamiPF00211. Guanylate_cyc. 1 hit.
PF07700. HNOB. 1 hit.
PF07701. HNOBA. 1 hit.
[Graphical view]
SMARTiSM00044. CYCc. 1 hit.
[Graphical view]
SUPFAMiSSF111126. SSF111126. 1 hit.
SSF55073. SSF55073. 1 hit.
PROSITEiPS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and functional expression of the rat alpha(2) subunit of soluble guanylyl cyclase."
    Koglin M., Behrends S.
    Biochim. Biophys. Acta 1494:286-289(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar Kyoto.
    Tissue: Aorta.
  2. "Rat soluble guanylate cyclase alpha 2 subunit."
    Yao Y., Yamamoto T., Suzuki N.
    Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiGCYA2_RAT
AccessioniPrimary (citable) accession number: Q9WVI4
Secondary accession number(s): Q54A43
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2001
Last sequence update: March 1, 2001
Last modified: June 8, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

There are two types of guanylate cyclases: soluble forms and membrane-associated receptor forms.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.