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Protein

Guanylate cyclase soluble subunit alpha-2

Gene

Gucy1a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Has guanylyl cyclase on binding to the beta-1 subunit.

Miscellaneous

There are two types of guanylate cyclases: soluble forms and membrane-associated receptor forms.

Catalytic activityi

GTP = 3',5'-cyclic GMP + diphosphate.

Enzyme regulationi

Activated by nitric oxide in the presence of magnesium or manganese ions.By similarity

GO - Molecular functioni

  • GTP binding Source: UniProtKB-KW
  • guanylate cyclase activity Source: RGD
  • heme binding Source: InterPro
  • protein heterodimerization activity Source: RGD

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processcGMP biosynthesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-392154. Nitric oxide stimulates guanylate cyclase.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanylate cyclase soluble subunit alpha-2 (EC:4.6.1.2)
Short name:
GCS-alpha-2
Gene namesi
Name:Gucy1a2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi621655. Gucy1a2.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000741141 – 730Guanylate cyclase soluble subunit alpha-2Add BLAST730

Proteomic databases

PaxDbiQ9WVI4.
PRIDEiQ9WVI4.

PTM databases

iPTMnetiQ9WVI4.
PhosphoSitePlusiQ9WVI4.

Expressioni

Gene expression databases

BgeeiENSRNOG00000029876.
GenevisibleiQ9WVI4. RN.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta chain.By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein heterodimerization activity Source: RGD

Protein-protein interaction databases

IntActiQ9WVI4. 5 interactors.
MINTiMINT-1782739.
STRINGi10116.ENSRNOP00000040361.

Structurei

3D structure databases

ProteinModelPortaliQ9WVI4.
SMRiQ9WVI4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini519 – 646Guanylate cyclasePROSITE-ProRule annotationAdd BLAST128

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi50 – 72Ala-richAdd BLAST23
Compositional biasi64 – 72Poly-Ala9

Sequence similaritiesi

Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4171. Eukaryota.
COG2114. LUCA.
GeneTreeiENSGT00760000118959.
HOVERGENiHBG106603.
InParanoidiQ9WVI4.
KOiK12318.
OMAiQNWPNIH.
OrthoDBiEOG091G04KV.
PhylomeDBiQ9WVI4.

Family and domain databases

Gene3Di3.30.70.1230. 1 hit.
InterProiView protein in InterPro
IPR001054. A/G_cyclase.
IPR018297. A/G_cyclase_CS.
IPR011644. Heme_NO-bd.
IPR011645. HNOB_dom_associated.
IPR024096. NO_sig/Golgi_transp_ligand-bd.
IPR029787. Nucleotide_cyclase.
PfamiView protein in Pfam
PF00211. Guanylate_cyc. 1 hit.
PF07700. HNOB. 1 hit.
PF07701. HNOBA. 1 hit.
SMARTiView protein in SMART
SM00044. CYCc. 1 hit.
SUPFAMiSSF111126. SSF111126. 1 hit.
SSF55073. SSF55073. 1 hit.
PROSITEiView protein in PROSITE
PS00452. GUANYLATE_CYCLASE_1. 1 hit.
PS50125. GUANYLATE_CYCLASE_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9WVI4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRRKISSES FSSLGSDYLE TSPEEEGECP LSKLCWNGSR SPPGPPGSRA
60 70 80 90 100
AAMAATPVPA ASVAAAAAAV AAGSKRAQRR RRVNLDSLGE SISLLTAPSP
110 120 130 140 150
QTIHMTLKRT LQYYEHQVIG YRDAEKNFHN ISNRCSSADH SNKEEIEDVS
160 170 180 190 200
GILRCTANVL GLKFQEIQER FGEEFFKICF DENERVLRAV GSTLQDFFNG
210 220 230 240 250
FDALLEHIRT SFGKQATLES PSFLCKELPE GTLKLHYFHP HHTVGFAMLG
260 270 280 290 300
MIKAAGKRIY HLNVEVEQIE NEKFCSDGST PSNYSCLTFL IKECETTQIT
310 320 330 340 350
KNIPQGTSQI PTDLRISINT FCRTFPFHLM FDPNMVVLQL GEGLRKQLRC
360 370 380 390 400
DNHKVLKFED CFEIVSPKVN ATFDRVLLRL STPFVIRTKP EASGTDNEDK
410 420 430 440 450
VMEIKGQMIH VPESNAILFL GSPCVDKLDE LIGRGLHLSD IPIHDATRDV
460 470 480 490 500
ILVGEQAKAQ DGLKKRMDKL KATLEKTHQA LEEEKKKTVD LLYSIFPGDV
510 520 530 540 550
AQQLWQRQQV QARKFDDVTM LFSDIVGFTA ICAQCTPMQV ISMLNELYTR
560 570 580 590 600
FDHQCGFLDI YKVETIGDAY CVASGLHRKS LCHAKPIALM ALKMMELSEE
610 620 630 640 650
VLTPDGRPIQ MRIGIHSGSV LAGVVGVRMP RYCLFGNNVT LASKFESGSH
660 670 680 690 700
PRRINISPTT YQLLKREDSF TFIPRSREEL PDNFPKEIPG VCYFLELRTG
710 720 730
PKPPKPSLSS SRIKKVSYNI GTMFLRETSL
Length:730
Mass (Da):81,787
Last modified:March 1, 2001 - v2
Checksum:iB8D790BFF81FB8F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF109963 mRNA. Translation: AAD42949.2.
AB096080 mRNA. Translation: BAC24017.1.
RefSeqiNP_076446.1. NM_023956.1.
UniGeneiRn.209304.

Genome annotation databases

EnsembliENSRNOT00000046058; ENSRNOP00000040361; ENSRNOG00000029876.
GeneIDi66012.
KEGGirno:66012.
UCSCiRGD:621655. rat.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGCYA2_RAT
AccessioniPrimary (citable) accession number: Q9WVI4
Secondary accession number(s): Q54A43
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2001
Last sequence update: March 1, 2001
Last modified: June 7, 2017
This is version 127 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families