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Protein

Fibulin-5

Gene

Fbln5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Essential for elastic fiber formation, is involved in the assembly of continuous elastin (ELN) polymer and promotes the interaction of microfibrils and ELN. Stabilizes and organizes elastic fibers in the skin, lung and vasculature. Promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. Vascular ligand for integrin receptors which may play a role in vascular development and remodeling.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiR-RNO-1566948. Elastic fibre formation.
R-RNO-2129379. Molecules associated with elastic fibres.

Names & Taxonomyi

Protein namesi
Recommended name:
Fibulin-5
Short name:
FIBL-5
Alternative name(s):
Developmental arteries and neural crest EGF-like protein
Short name:
Dance
Embryonic vascular EGF repeat-containing protein
Short name:
EVEC
Gene namesi
Name:Fbln5
Synonyms:Dance
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 6

Organism-specific databases

RGDi2594. Fbln5.

Subcellular locationi

  • Secreted By similarity
  • Secretedextracellular spaceextracellular matrix By similarity

  • Note: co-localizes with ELN in elastic fibers.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Chaini24 – 448425Fibulin-5PRO_0000007579Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi46 ↔ 59PROSITE-ProRule annotation
Disulfide bondi53 ↔ 68PROSITE-ProRule annotation
Disulfide bondi131 ↔ 144PROSITE-ProRule annotation
Disulfide bondi138 ↔ 153PROSITE-ProRule annotation
Disulfide bondi155 ↔ 166PROSITE-ProRule annotation
Disulfide bondi172 ↔ 181PROSITE-ProRule annotation
Disulfide bondi177 ↔ 190PROSITE-ProRule annotation
Disulfide bondi192 ↔ 205PROSITE-ProRule annotation
Disulfide bondi211 ↔ 221PROSITE-ProRule annotation
Disulfide bondi217 ↔ 230PROSITE-ProRule annotation
Disulfide bondi232 ↔ 245PROSITE-ProRule annotation
Disulfide bondi251 ↔ 262PROSITE-ProRule annotation
Disulfide bondi258 ↔ 271PROSITE-ProRule annotation
Disulfide bondi273 ↔ 286PROSITE-ProRule annotation
Glycosylationi283 – 2831N-linked (GlcNAc...)Sequence analysis
Disulfide bondi292 ↔ 305PROSITE-ProRule annotation
Glycosylationi296 – 2961N-linked (GlcNAc...)Sequence analysis
Disulfide bondi299 ↔ 314PROSITE-ProRule annotation
Disulfide bondi320 ↔ 332PROSITE-ProRule annotation

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9WVH8.
PRIDEiQ9WVH8.

Expressioni

Gene expression databases

GenevisibleiQ9WVH8. RN.

Interactioni

Subunit structurei

Homodimer. Monomer, homodimerizes in presence of Ca2+. Interacts with ELN. Interacts (via N-terminus) with the integrins ITGAV/ITGB3, ITGAV/ITGB5 and ITGA9/ITGB1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000007004.

Structurei

3D structure databases

ProteinModelPortaliQ9WVH8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini42 – 8241EGF-like 1; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini127 – 16741EGF-like 2; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini168 – 20639EGF-like 3; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini207 – 24640EGF-like 4; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini247 – 28741EGF-like 5; calcium-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini288 – 33346EGF-like 6; calcium-bindingPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi54 – 563Cell attachment siteSequence analysis

Sequence similaritiesi

Belongs to the fibulin family.Curated
Contains 6 EGF-like domains.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IR76. Eukaryota.
ENOG41104WD. LUCA.
GeneTreeiENSGT00760000118806.
HOGENOMiHOG000234337.
HOVERGENiHBG051560.
InParanoidiQ9WVH8.
KOiK17340.
OMAiPRDIQLD.
OrthoDBiEOG7W9RTF.

Family and domain databases

InterProiIPR026823. cEGF.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR009030. Growth_fac_rcpt_.
[Graphical view]
PfamiPF12662. cEGF. 2 hits.
PF07645. EGF_CA. 2 hits.
[Graphical view]
SMARTiSM00181. EGF. 5 hits.
SM00179. EGF_CA. 6 hits.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
PROSITEiPS00010. ASX_HYDROXYL. 4 hits.
PS01186. EGF_2. 4 hits.
PS50026. EGF_3. 5 hits.
PS01187. EGF_CA. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9WVH8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGLKRILTV TILALWLPHP GNAQQQCTNG FDLDRQTGQC LDIDECRTIP
60 70 80 90 100
EACRGDMMCV NQNGGYLCIP RTNPVYRGPY SNPYSTSYSG PYPAAAPPVP
110 120 130 140 150
ASNYPTISRP LVCRFGYQMD EGNQCVDVDE CATDSHQCNP TQICINTEGG
160 170 180 190 200
YTCSCTDGYW LLEGQCLDID ECRYGYCQQL CANVPGSYSC TCNPGFTLND
210 220 230 240 250
DGRSCQDVNE CETENPCVQT CVNTYGSFIC RCDPGYELEE DGIHCSDMDE
260 270 280 290 300
CSFSEFLCQH ECVNQPGSYF CSCPPGYVLL EDNRSCQDIN ECEHRNHTCT
310 320 330 340 350
PLQTCYNLQG GFKCIDPIVC EEPYLLIGDN RCMCPAENTG CRDQPFTILF
360 370 380 390 400
RDMDVVSGRS VPADIFQMQA TTRYPGAYYI FQIKSGNEGR EFYMRQTGPI
410 420 430 440
SATLVMTRPI KGPRDIQLDL EMITVNTVIN FRGSSVIRLR IYVSQYPF
Length:448
Mass (Da):50,160
Last modified:November 1, 1999 - v1
Checksum:iE6BC68F7BF14B714
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti238 – 2381L → P in AAD25101 (PubMed:10347091).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF112153 mRNA. Translation: AAD41769.1.
AF137350 mRNA. Translation: AAD25101.1.
BC078845 mRNA. Translation: AAH78845.1.
RefSeqiNP_062026.2. NM_019153.3.
UniGeneiRn.1699.

Genome annotation databases

EnsembliENSRNOT00000007004; ENSRNOP00000007004; ENSRNOG00000050539.
ENSRNOT00000084815; ENSRNOP00000069614; ENSRNOG00000050539.
GeneIDi29158.
KEGGirno:29158.
UCSCiRGD:2594. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF112153 mRNA. Translation: AAD41769.1.
AF137350 mRNA. Translation: AAD25101.1.
BC078845 mRNA. Translation: AAH78845.1.
RefSeqiNP_062026.2. NM_019153.3.
UniGeneiRn.1699.

3D structure databases

ProteinModelPortaliQ9WVH8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000007004.

Proteomic databases

PaxDbiQ9WVH8.
PRIDEiQ9WVH8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000007004; ENSRNOP00000007004; ENSRNOG00000050539.
ENSRNOT00000084815; ENSRNOP00000069614; ENSRNOG00000050539.
GeneIDi29158.
KEGGirno:29158.
UCSCiRGD:2594. rat.

Organism-specific databases

CTDi10516.
RGDi2594. Fbln5.

Phylogenomic databases

eggNOGiENOG410IR76. Eukaryota.
ENOG41104WD. LUCA.
GeneTreeiENSGT00760000118806.
HOGENOMiHOG000234337.
HOVERGENiHBG051560.
InParanoidiQ9WVH8.
KOiK17340.
OMAiPRDIQLD.
OrthoDBiEOG7W9RTF.

Enzyme and pathway databases

ReactomeiR-RNO-1566948. Elastic fibre formation.
R-RNO-2129379. Molecules associated with elastic fibres.

Miscellaneous databases

PROiQ9WVH8.

Gene expression databases

GenevisibleiQ9WVH8. RN.

Family and domain databases

InterProiIPR026823. cEGF.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR009030. Growth_fac_rcpt_.
[Graphical view]
PfamiPF12662. cEGF. 2 hits.
PF07645. EGF_CA. 2 hits.
[Graphical view]
SMARTiSM00181. EGF. 5 hits.
SM00179. EGF_CA. 6 hits.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
PROSITEiPS00010. ASX_HYDROXYL. 4 hits.
PS01186. EGF_2. 4 hits.
PS50026. EGF_3. 5 hits.
PS01187. EGF_CA. 6 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "DANCE, a novel secreted RGD protein expressed in developing, atherosclerotic, and balloon-injured arteries."
    Nakamura T., Ruiz-Lozano P., Lindner V., Yabe D., Taniwaki M., Furukawa Y., Kobuke K., Tashiro K., Lu Z., Andon N.L., Schaub R., Matsumori A., Sasayama S., Chien K.R., Honjo T.
    J. Biol. Chem. 274:22476-22483(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "EVEC, a novel epidermal growth factor-like repeat-containing protein upregulated in embryonic and diseased adult vasculature."
    Kowal R.C., Richardson J.A., Miano J.M., Olson E.N.
    Circ. Res. 84:1166-1176(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.

Entry informationi

Entry nameiFBLN5_RAT
AccessioniPrimary (citable) accession number: Q9WVH8
Secondary accession number(s): Q9R284
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1999
Last modified: June 8, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.