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Protein

DNA polymerase epsilon catalytic subunit A

Gene

Pole

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Participates in DNA repair and in chromosomal DNA replication.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi2155 – 21551ZincBy similarity
Metal bindingi2158 – 21581ZincBy similarity
Metal bindingi2184 – 21841ZincBy similarity
Metal bindingi2187 – 21871ZincBy similarity
Metal bindingi2218 – 22181Iron-sulfur (4Fe-4S)By similarity
Metal bindingi2221 – 22211Iron-sulfur (4Fe-4S)By similarity
Metal bindingi2233 – 22331Iron-sulfur (4Fe-4S)By similarity
Metal bindingi2235 – 22351Iron-sulfur (4Fe-4S)By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri2155 – 218733CysA-typeAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA damage, DNA repair, DNA replication

Keywords - Ligandi

4Fe-4S, DNA-binding, Iron, Iron-sulfur, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-174430. Telomere C-strand synthesis initiation.
R-MMU-68952. DNA replication initiation.
R-MMU-68962. Activation of the pre-replicative complex.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase epsilon catalytic subunit A (EC:2.7.7.7)
Alternative name(s):
DNA polymerase II subunit A
Gene namesi
Name:Pole
Synonyms:Pole1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1196391. Pole.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 22832283DNA polymerase epsilon catalytic subunit APRO_0000046456Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1184 – 11841PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9WVF7.
MaxQBiQ9WVF7.
PaxDbiQ9WVF7.
PRIDEiQ9WVF7.

PTM databases

iPTMnetiQ9WVF7.
PhosphoSiteiQ9WVF7.

Expressioni

Gene expression databases

BgeeiQ9WVF7.
CleanExiMM_POLE.
ExpressionAtlasiQ9WVF7. baseline and differential.
GenevisibleiQ9WVF7. MM.

Interactioni

Subunit structurei

Catalytic and central component of the epsilon DNA polymerase complex consisting of four subunits: POLE, POLE2, POLE3 and POLE4. Interacts with RAD17 and TOPBP1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi202292. 2 interactions.
STRINGi10090.ENSMUSP00000007296.

Structurei

3D structure databases

ProteinModelPortaliQ9WVF7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi2218 – 223518CysB motifAdd
BLAST

Domaini

The DNA polymerase activity domain resides in the N-terminal half of the protein, while the C-terminus is necessary for complexing subunits B and C. The C-terminus may also regulate the catalytic activities of the enzyme.
The CysB motif binds 1 4Fe-4S cluster and is required for the formation of polymerase complexes.By similarity

Sequence similaritiesi

Belongs to the DNA polymerase type-B family.Curated
Contains 1 CysA-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri2155 – 218733CysA-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1798. Eukaryota.
COG0417. LUCA.
GeneTreeiENSGT00390000010194.
HOGENOMiHOG000196287.
HOVERGENiHBG051398.
InParanoidiQ9WVF7.
KOiK02324.
OMAiIQDDGGM.
OrthoDBiEOG7F24RX.
TreeFamiTF105017.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR006172. DNA-dir_DNA_pol_B.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR013697. DNA_pol_e_suA_C.
IPR029703. POL2.
IPR012337. RNaseH-like_dom.
[Graphical view]
PANTHERiPTHR10670. PTHR10670. 2 hits.
PfamiPF00136. DNA_pol_B. 1 hit.
PF03104. DNA_pol_B_exo1. 1 hit.
PF08490. DUF1744. 1 hit.
[Graphical view]
SMARTiSM01159. DUF1744. 1 hit.
SM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 2 hits.

Sequencei

Sequence statusi: Complete.

Q9WVF7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVLRNSGRRH PEPGADGEGS RDDGPSSSVS ALKRLERSQW TDKMDLRFGF
60 70 80 90 100
ERLKEPGERT GWLINMHPTE ILDEDKRLVS AVDYYFIQDD GSRFKVALPY
110 120 130 140 150
MPYFYIAARK GCDREVSSFL SKKFQGKIAK LENVPKEDLD LPNHLVGLKR
160 170 180 190 200
SYIKLSFHTV EDLVKVRKEI SPAVKKNREQ DHASDEYTTM LSSILQGGSV
210 220 230 240 250
ITDEDETSKK IADQLDNIVD MREYDVPYHI RLSIDLRIHV AHWYNVRFRG
260 270 280 290 300
NAFPVEITRR DDLVERPDPV VLAFDIETTK LPLKFPDAET DQIMMISYMI
310 320 330 340 350
DGQGYLITNR EIVSEDIEDF EFTPKPEYEG PFCVFNEPDE VHLIQRWFEH
360 370 380 390 400
IQETKPTIMV TYNGDFFDWP FVEARAAIHG LSMYQEIGFQ KDSQGEYKAP
410 420 430 440 450
QCIHMDCLRW VKRDSYLPVG SHNLKAAAKA KLGYDPVELD PEDMCRMATE
460 470 480 490 500
QPQTLATYSV SDAVATYYLY MKYVHPFIFA LCTIIPMEPD EVLRKGSGTL
510 520 530 540 550
CEALLMVQAF HANIIFPNKQ EQEFNKLTDD GHMLDAETYV GGHVEALESG
560 570 580 590 600
VFRSDIPCRF RMNPAAFDFL LQRVEKTMRH AIEEEEKVPV EQATNFQEVC
610 620 630 640 650
EQIKTKLTSL KDVPNRIECP LIYHLDVGAM YPNIILTNRL QPSAIVDEAT
660 670 680 690 700
CAACDFNKPG ASCQRKMAWQ WRGEFMPASR SEYHRIQHQL ESEKFPPLFP
710 720 730 740 750
EGPARAFHEL SREEQAKYEK RRLADYCRKA YKKIHVTKVE ERLTTICQRE
760 770 780 790 800
NSFYVDTVRA FRDRRYEFKG LHKVWKKKLS AAVEVGDASE VKRCKNMEIL
810 820 830 840 850
YDSLQLAHKC ILNSFYGYVM RKGARWYSME MAGIVCFTGA NIITQARELI
860 870 880 890 900
EQIGRPLELD TDGIWCVLPN SFPENFVIKT TNAKKPKLTI SYPGAMLNIM
910 920 930 940 950
VKEGFTNHQY QELTEPSSLT YVTHSENSIF FEVDGPYLAM ILPASKEEGK
960 970 980 990 1000
KLKKRYAVFN EDGSLAELKG FEVKRRGELQ LIKIFQSSVF EAFLKGSTLE
1010 1020 1030 1040 1050
EVYGSVAKVA DYWLDVLYSK AANMPDSELF ELISENRSMS RKLEDYGEQK
1060 1070 1080 1090 1100
STSISTAKRL AEFLGDQMVK DAGLSCRYII SRKPEGSPVT ERAIPLAIFQ
1110 1120 1130 1140 1150
AEPTVRKHFL RKWLKSSSLQ DFDIRTILDW DYYIERLGSA IQKIITIPAA
1160 1170 1180 1190 1200
LQQVKNPVPR VKHPDWLHKK LLEKNDIYKQ KKISELFVLE GKRQIVMAQA
1210 1220 1230 1240 1250
SENSLSLCTP DMEDIGLTKP HHSTVPVATK RKRVWETQKE SQDIALTVPW
1260 1270 1280 1290 1300
QEVLGQPPSL GTTQEEWLVW LQFHKKKWQL QAQQRLALRK KQRLESAEDM
1310 1320 1330 1340 1350
PRLGPIREGP STGLGSFLRR TARSIMDLPW QIIQISETRQ AGLFRLWAII
1360 1370 1380 1390 1400
GNDLHCIKLS IPRVFYVNQR VAKAEDGPAY RKVNRALPRS NIVYNLYEYS
1410 1420 1430 1440 1450
VPEDMYQEHI NEINTELSVP DIEGVYETQV PLLFRALVQL GCVCVVNKQL
1460 1470 1480 1490 1500
TRHLSGWEAE TFALEHLEMR SLAQFSYLEP GSIRHIYLYH HTQGHKALFG
1510 1520 1530 1540 1550
VFIPSQRRAS VFVLDTVRSN QMPGLSALYS SEHSLLLDKV DPKLLPPPKH
1560 1570 1580 1590 1600
TFEVRAETNL KTICRAIQRF LLAYKEERRG PTLIAVQSSW ELCRLTSEIP
1610 1620 1630 1640 1650
VLEEFPLVPI RVADKISYAV LDWQRHGARR MIRHYLNLDL CLSQAFEMSR
1660 1670 1680 1690 1700
YFHIPVGNLP EDISTFGSDL FFARHLQHHN HLLWLSPTSR PDLGGKEADD
1710 1720 1730 1740 1750
NRLVMEFDDR ATVEINSSGC YSTVCVELDI QNLAVNTILQ SHHVNDMEGA
1760 1770 1780 1790 1800
GSMGISFDVI QQASLEDMVT GNQAASALAN YDETALCSST FRILKSMVVG
1810 1820 1830 1840 1850
WVKEITQYHN IYADNQVMHF YRWLQSPCSL LHDPALHRTL HNMMKKLFLQ
1860 1870 1880 1890 1900
LIAEFKRLGS SVVYANFNRI ILCTKKRRIE DALAYVEYIT NSIHSKEIFH
1910 1920 1930 1940 1950
SLTISFSRCW EFLLWMDPSN YGGIKGKVPS SIHCGQVKEQ DSQAREETDE
1960 1970 1980 1990 2000
EEEDKEKDEE EEGMGESEVE DLLENNWNIL QFLPQAASCQ SYFLMIVSAY
2010 2020 2030 2040 2050
IVAVYQSMKE ELRHSAPGST PVKRKGASQF SQESEGATGS LPGMITFSQD
2060 2070 2080 2090 2100
YVANELTQSF FTITQKIQKK VTGSRNTTEP SEMFPVLPGS HLLLNNPALE
2110 2120 2130 2140 2150
FIKYVCKVLS LDTNITNQVN KLNRDLLRLV DVGEFSEEAQ FRDPCHSYVL
2160 2170 2180 2190 2200
PEVICHSCNF CRDLDLCKDS SFSQDGAILP QWLCSNCQAP YDSSAIESAL
2210 2220 2230 2240 2250
VEALQRKLMA FTLQDLVCLK CRGMKETHMP VYCSCAGDFT LTIRTEVFME
2260 2270 2280
QIRIFQNIAK YYSMSYLQET IEWLLQTSPV SNC
Length:2,283
Mass (Da):262,100
Last modified:July 27, 2011 - v3
Checksum:i746B2A94D2E8A151
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti284 – 2841K → T in AAD46482 (PubMed:10366722).Curated
Sequence conflicti533 – 5331M → V in AAD46482 (PubMed:10366722).Curated
Sequence conflicti533 – 5331M → V in AAD45244 (PubMed:10366722).Curated
Sequence conflicti1309 – 13091G → E in AAD45244 (PubMed:10366722).Curated
Sequence conflicti1320 – 13201R → K in AAD46482 (PubMed:10366722).Curated
Sequence conflicti1386 – 13883ALP → GLFL in AAD45244 (PubMed:10366722).Curated
Sequence conflicti1561 – 15611K → E in AAD46482 (PubMed:10366722).Curated
Sequence conflicti1561 – 15611K → E in AAD45244 (PubMed:10366722).Curated
Sequence conflicti1665 – 16651T → I in AAD45244 (PubMed:10366722).Curated
Sequence conflicti2086 – 20861V → I in AAD46482 (PubMed:10366722).Curated
Sequence conflicti2111 – 21111L → Q in AAD45244 (PubMed:10366722).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF123502 mRNA. Translation: AAD45244.1.
AF126398
, AF126377, AF126378, AF126379, AF126380, AF126381, AF126382, AF126383, AF126384, AF126385, AF126386, AF126387, AF126388, AF126389, AF126390, AF126391, AF126392, AF126393, AF126394, AF126395, AF126396, AF126397 Genomic DNA. Translation: AAD46482.1.
AC118260 Genomic DNA. No translation available.
AC123699 Genomic DNA. No translation available.
CCDSiCCDS19525.1.
RefSeqiNP_035262.2. NM_011132.2.
UniGeneiMm.35061.

Genome annotation databases

EnsembliENSMUST00000007296; ENSMUSP00000007296; ENSMUSG00000007080.
GeneIDi18973.
KEGGimmu:18973.
UCSCiuc008yqm.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF123502 mRNA. Translation: AAD45244.1.
AF126398
, AF126377, AF126378, AF126379, AF126380, AF126381, AF126382, AF126383, AF126384, AF126385, AF126386, AF126387, AF126388, AF126389, AF126390, AF126391, AF126392, AF126393, AF126394, AF126395, AF126396, AF126397 Genomic DNA. Translation: AAD46482.1.
AC118260 Genomic DNA. No translation available.
AC123699 Genomic DNA. No translation available.
CCDSiCCDS19525.1.
RefSeqiNP_035262.2. NM_011132.2.
UniGeneiMm.35061.

3D structure databases

ProteinModelPortaliQ9WVF7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202292. 2 interactions.
STRINGi10090.ENSMUSP00000007296.

PTM databases

iPTMnetiQ9WVF7.
PhosphoSiteiQ9WVF7.

Proteomic databases

EPDiQ9WVF7.
MaxQBiQ9WVF7.
PaxDbiQ9WVF7.
PRIDEiQ9WVF7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000007296; ENSMUSP00000007296; ENSMUSG00000007080.
GeneIDi18973.
KEGGimmu:18973.
UCSCiuc008yqm.2. mouse.

Organism-specific databases

CTDi5426.
MGIiMGI:1196391. Pole.

Phylogenomic databases

eggNOGiKOG1798. Eukaryota.
COG0417. LUCA.
GeneTreeiENSGT00390000010194.
HOGENOMiHOG000196287.
HOVERGENiHBG051398.
InParanoidiQ9WVF7.
KOiK02324.
OMAiIQDDGGM.
OrthoDBiEOG7F24RX.
TreeFamiTF105017.

Enzyme and pathway databases

ReactomeiR-MMU-174430. Telomere C-strand synthesis initiation.
R-MMU-68952. DNA replication initiation.
R-MMU-68962. Activation of the pre-replicative complex.

Miscellaneous databases

NextBioi295328.
PROiQ9WVF7.
SOURCEiSearch...

Gene expression databases

BgeeiQ9WVF7.
CleanExiMM_POLE.
ExpressionAtlasiQ9WVF7. baseline and differential.
GenevisibleiQ9WVF7. MM.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR006172. DNA-dir_DNA_pol_B.
IPR006133. DNA-dir_DNA_pol_B_exonuc.
IPR006134. DNA-dir_DNA_pol_B_multi_dom.
IPR013697. DNA_pol_e_suA_C.
IPR029703. POL2.
IPR012337. RNaseH-like_dom.
[Graphical view]
PANTHERiPTHR10670. PTHR10670. 2 hits.
PfamiPF00136. DNA_pol_B. 1 hit.
PF03104. DNA_pol_B_exo1. 1 hit.
PF08490. DUF1744. 1 hit.
[Graphical view]
SMARTiSM01159. DUF1744. 1 hit.
SM00486. POLBc. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA and structural organization of the gene Pole1 for the mouse DNA polymerase epsilon catalytic subunit."
    Huang D., Knuuti R., Palosaari H., Pospiech H., Syvaoja J.E.
    Biochim. Biophys. Acta 1445:363-371(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Spleen and Testis.

Entry informationi

Entry nameiDPOE1_MOUSE
AccessioniPrimary (citable) accession number: Q9WVF7
Secondary accession number(s): E9QKW1, Q9QX50
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2001
Last sequence update: July 27, 2011
Last modified: May 11, 2016
This is version 125 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.