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Protein

Intersectin-1

Gene

Itsn1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein that may provide indirect link between the endocytic membrane traffic and the actin assembly machinery. May regulate the formation of clathrin-coated vesicles. Inhibits ARHGAP31 activity toward RAC1.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi66 – 78131PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi267 – 279132PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • endocytosis Source: UniProtKB-KW
  • exocytosis Source: RGD
Complete GO annotation...

Keywords - Biological processi

Endocytosis

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Intersectin-1
Alternative name(s):
EH domain and SH3 domain regulator of endocytosis 1
Gene namesi
Name:Itsn1
Synonyms:Ehsh1, Itsn
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2935. Itsn1.

Subcellular locationi

GO - Cellular componenti

  • cell junction Source: UniProtKB-KW
  • coated pit Source: UniProtKB-SubCell
  • lamellipodium Source: UniProtKB-SubCell
  • terminal bouton Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell projection, Coated pit, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12171217Intersectin-1PRO_0000080959Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei203 – 2031PhosphoserineCombined sources
Modified residuei318 – 3181PhosphoserineBy similarity
Modified residuei334 – 3341PhosphoserineCombined sources
Modified residuei335 – 3351PhosphoserineCombined sources
Modified residuei685 – 6851PhosphoserineCombined sources
Modified residuei894 – 8941PhosphothreonineBy similarity
Modified residuei898 – 8981PhosphoserineBy similarity
Modified residuei899 – 8991PhosphoserineCombined sources
Modified residuei901 – 9011PhosphoserineCombined sources
Modified residuei975 – 9751PhosphoserineCombined sources
Modified residuei981 – 9811PhosphothreonineBy similarity
Modified residuei992 – 9921PhosphoserineBy similarity
Modified residuei1134 – 11341PhosphoserineCombined sources
Modified residuei1141 – 11411PhosphothreonineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9WVE9.
PRIDEiQ9WVE9.

PTM databases

PhosphoSiteiQ9WVE9.

Expressioni

Tissue specificityi

Highly expressed in brain.

Interactioni

Subunit structurei

Interacts with dynamin, CDC42, SNAP25 and SNAP23. Clusters several dynamin in a manner that is regulated by alternative splicing. Interacts with clathrin-associated proteins and other components of the endocytic machinery, such as SPIN90, EPS15, EPN1, EPN2, STON2, FCHO1, FCHO2 and DAB2. Interacts (via SH3 domains) with REPS1 and SGIP1. Interacts with ARHGAP31. Interacts with ADAM15.1 Publication

Protein-protein interaction databases

BioGridi248132. 4 interactions.
IntActiQ9WVE9. 3 interactions.
MINTiMINT-95913.
STRINGi10116.ENSRNOP00000045513.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3HS9X-ray2.15P844-855[»]
ProteinModelPortaliQ9WVE9.
SMRiQ9WVE9. Positions 910-968, 1002-1057, 1067-1211.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9WVE9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 10989EH 1PROSITE-ProRule annotationAdd
BLAST
Domaini53 – 8836EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini221 – 31090EH 2PROSITE-ProRule annotationAdd
BLAST
Domaini254 – 28936EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini737 – 80367SH3 1PROSITE-ProRule annotationAdd
BLAST
Domaini910 – 96859SH3 2PROSITE-ProRule annotationAdd
BLAST
Domaini999 – 105759SH3 3PROSITE-ProRule annotationAdd
BLAST
Domaini1071 – 113565SH3 4PROSITE-ProRule annotationAdd
BLAST
Domaini1152 – 121160SH3 5PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni326 – 702377KLERQAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili350 – 670321Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi321 – 3244Poly-Ser

Domaini

SH3-1 and SH3-4 bind to ARHGAP31 (By similarity). SH3-3, SH3-4 and SH3-5, but not SH3-1 and SH3-2 domains, bind to dynamin.By similarity
The KLERQ domain binds to SNAP-25 and SNAP-23.

Sequence similaritiesi

Contains 2 EF-hand domains.PROSITE-ProRule annotation
Contains 2 EH domains.PROSITE-ProRule annotation
Contains 5 SH3 domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat, SH3 domain

Phylogenomic databases

eggNOGiKOG1029. Eukaryota.
ENOG410XPRA. LUCA.
HOGENOMiHOG000010175.
HOVERGENiHBG052159.
InParanoidiQ9WVE9.
PhylomeDBiQ9WVE9.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000261. EH_dom.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF12763. EF-hand_4. 2 hits.
PF00018. SH3_1. 1 hit.
PF07653. SH3_2. 1 hit.
PF14604. SH3_9. 3 hits.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00054. EFh. 2 hits.
SM00027. EH. 2 hits.
SM00326. SH3. 5 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 2 hits.
SSF50044. SSF50044. 5 hits.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS50031. EH. 2 hits.
PS50002. SH3. 5 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: Q9WVE9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQFPTPFGG SLDIWAITVE ERAKHDQQFQ SLKPISGFIT GDQARNFFFQ
60 70 80 90 100
SGLPQPVLAQ IWALADMNKD GRMDQVEFSI AMKLIKLKLQ GYQLPPALPP
110 120 130 140 150
VMKQQPAAIS SAPAFGIGGM AGMPPLTAVA PVPMGSIPVV GMSPPLVSSV
160 170 180 190 200
PQAAVPPLAN GAPPVIQPLP AFAHPAATLP KSSSFSRSGP GSQLNTKLQK
210 220 230 240 250
AQSFDVASAP AAAEWAVPQS SRLKYRQLFN SHDKTMSGHL TGPQARTILM
260 270 280 290 300
QSSLPQAQLA SIWNLSDIDQ DGKLTAEEFI LAMHLIDVAM SGQPLPPVLP
310 320 330 340 350
PEYIPPSFRR VRSGSGMSVI SSSSADQRLP EEPSSEDEQQ VEKKLPVTFE
360 370 380 390 400
DKKRENFERG NLELEKRRQA LLEQQRKEQE RLAQLERAEQ ERKERERQEQ
410 420 430 440 450
ERKRQLELEK QLEKQRELER QREEERRKEI ERREAAKREL ERQRQLEWER
460 470 480 490 500
NRRQELLTQR NKDQEGIVVL KARRKTLEFE LEALNDKKHQ LEGKLQDIRC
510 520 530 540 550
RLATQRQEIE STNKSRELRI AEITHLQQQL QESQQMLGRL IPEKQILSDQ
560 570 580 590 600
LKQVQQNSLH RDSLLTLKRA LEAKELARQQ LREQLDEVEK ETRSKLQEID
610 620 630 640 650
VFNNQLKELR EIHSKQQLQK QRSIEAERLK QKEQERKSLE LEKQKEEGQR
660 670 680 690 700
RVQERDKQWQ EHVQQEEQQR PRKPHEEDKL KREDSVKKKE AEERAKPEVQ
710 720 730 740 750
DKQSRLFHPH QEPAKPAQAP WPTTEKGPLT ISAQESAKVV YYRALYPFES
760 770 780 790 800
RSHDEITIQP GDIVMVKGEW VDESQTGEPG WLGGEPKGKT GWFPANYAEK
810 820 830 840 850
IPENEIPTPA KPVTDLTSAP APKLALRETP APLPVTSSEP STTPNNWADF
860 870 880 890 900
SSTWPSSTNE KPETDNWDTW AAQPSLTVPS AGQLRQRSAF TPATATGSSP
910 920 930 940 950
SPVLGQGEKV EGLQAQALYP WRAKKDNHLN FNKSDVITVL EQQDMWWFGE
960 970 980 990 1000
VQGQKGWFPK SYVKLISGPV RKSTSIDTGP TEAPSSLKRV ASPAAKPAIP
1010 1020 1030 1040 1050
GEEFVAMYTY ESSEHGDLTF QQGHVIVVTK KDGDWWTGTV GETSGVFPSN
1060 1070 1080 1090 1100
YVRLKDSEGS GTAGKTGSLE KKPEIAQVIA SYTATGPEQL TLAPGQLILI
1110 1120 1130 1140 1150
RKKNPGGWWE GELQARGKKR QIGWFPANYV KLLSPGTSKI TPTELPKTAV
1160 1170 1180 1190 1200
QPAVCQVIGM YDYTAQNDDE LAFSKGQIIN VLSKEDPDWW KGEVSGQVGL
1210
FPSNYVKLTT DMDPSQQ
Length:1,217
Mass (Da):137,155
Last modified:November 1, 1999 - v1
Checksum:i6C13238AE5A5B34B
GO
Isoform 2 (identifier: Q9WVE9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1003-1073: Missing.

Show »
Length:1,146
Mass (Da):129,443
Checksum:i48CD5E4B92DD276A
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1003 – 107371Missing in isoform 2. 1 PublicationVSP_004297Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF127798 mRNA. Translation: AAD30271.1.
AF132672 mRNA. Translation: AAD31026.1.
RefSeqiNP_062100.1. NM_019227.1. [Q9WVE9-1]
UniGeneiRn.48657.

Genome annotation databases

GeneIDi29491.
KEGGirno:29491.
UCSCiRGD:2935. rat. [Q9WVE9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF127798 mRNA. Translation: AAD30271.1.
AF132672 mRNA. Translation: AAD31026.1.
RefSeqiNP_062100.1. NM_019227.1. [Q9WVE9-1]
UniGeneiRn.48657.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3HS9X-ray2.15P844-855[»]
ProteinModelPortaliQ9WVE9.
SMRiQ9WVE9. Positions 910-968, 1002-1057, 1067-1211.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248132. 4 interactions.
IntActiQ9WVE9. 3 interactions.
MINTiMINT-95913.
STRINGi10116.ENSRNOP00000045513.

PTM databases

PhosphoSiteiQ9WVE9.

Proteomic databases

PaxDbiQ9WVE9.
PRIDEiQ9WVE9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29491.
KEGGirno:29491.
UCSCiRGD:2935. rat. [Q9WVE9-1]

Organism-specific databases

CTDi6453.
RGDi2935. Itsn1.

Phylogenomic databases

eggNOGiKOG1029. Eukaryota.
ENOG410XPRA. LUCA.
HOGENOMiHOG000010175.
HOVERGENiHBG052159.
InParanoidiQ9WVE9.
PhylomeDBiQ9WVE9.

Miscellaneous databases

EvolutionaryTraceiQ9WVE9.
NextBioi609367.
PROiQ9WVE9.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000261. EH_dom.
IPR011511. SH3_2.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF12763. EF-hand_4. 2 hits.
PF00018. SH3_1. 1 hit.
PF07653. SH3_2. 1 hit.
PF14604. SH3_9. 3 hits.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00054. EFh. 2 hits.
SM00027. EH. 2 hits.
SM00326. SH3. 5 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 2 hits.
SSF50044. SSF50044. 5 hits.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS50031. EH. 2 hits.
PS50002. SH3. 5 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "EHSH1/intersectin, a protein that contains EH and SH3 domains and binds to dynamin and SNAP-25. A protein connection between exocytosis and endocytosis?"
    Okamoto M., Schoch S., Suedhof T.C.
    J. Biol. Chem. 274:18446-18454(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    Tissue: Brain.
  2. "FCHo proteins are nucleators of clathrin-mediated endocytosis."
    Henne W.M., Boucrot E., Meinecke M., Evergren E., Vallis Y., Mittal R., McMahon H.T.
    Science 328:1281-1284(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH FCHO2.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-203; SER-334; SER-335; SER-685; SER-899; SER-901; SER-975; SER-1134 AND THR-1141, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiITSN1_RAT
AccessioniPrimary (citable) accession number: Q9WVE9
Secondary accession number(s): Q9WVE1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: November 1, 1999
Last modified: May 11, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.