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Q9WVE0 (AICDA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Single-stranded DNA cytosine deaminase

EC=3.5.4.38
Alternative name(s):
Activation-induced cytidine deaminase
Cytidine aminohydrolase
Gene names
Name:Aicda
Synonyms:Aid
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length198 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Single-stranded DNA-specific cytidine deaminase. Involved in somatic hypermutation, gene conversion, and class-switch recombination in B-lymphocytes. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Ref.2

Catalytic activity

Cytosine in single-stranded DNA + H2O = uracil in single-stranded DNA + NH3.

Cofactor

Zinc By similarity.

Subunit structure

Interacts with CTNNBL1; the interaction is important for the immunoglobulin switch activity of AICDA. Interacts (via its NLS) with KPNA1. Interacts with PKA/PRKACA and PRKAR1A/PKR1 By similarity. Interacts with SUPT6H, TRIM28 and NCL. Ref.3

Subcellular location

Nucleus By similarity. Cytoplasm By similarity. Note: Predominantly cytoplasmic but shuttles between the nucleus and the cytoplasm By similarity.

Post-translational modification

Ser-38 is the major site whereas Thr-27 is the minor site of phosphorylation. Phosphorylation regulates its class-switch recombination activity By similarity.

Sequence similarities

Belongs to the cytidine and deoxycytidylate deaminase family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

GADD45AP245223EBI-3835567,EBI-448167From a different organism.
Ptbp2Q91Z314EBI-3835567,EBI-647632
Top2bQ645113EBI-3835567,EBI-2325586

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 198198Single-stranded DNA cytosine deaminase
PRO_0000171688

Regions

Region1 – 3030Nuclear localization signal By similarity
Region2 – 2625Interaction with SUPT6H By similarity
Region39 – 424Important for interaction with CTNNBL1 By similarity
Region183 – 19816Nuclear export signal By similarity

Sites

Active site581Proton donor By similarity
Metal binding561Zinc; catalytic By similarity
Metal binding871Zinc; catalytic By similarity
Metal binding901Zinc; catalytic By similarity

Amino acid modifications

Modified residue271Phosphothreonine; by PKA By similarity
Modified residue381Phosphoserine; by PKA By similarity

Experimental info

Mutagenesis561H → R: Loss of the cytidine deaminase activity; when associated with Q-58. Ref.2
Mutagenesis581E → Q: Loss of the cytidine deaminase activity; when associated with R-56. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9WVE0 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 18A3BA10CA54BEB2

FASTA19824,031
        10         20         30         40         50         60 
MDSLLMKQKK FLYHFKNVRW AKGRHETYLC YVVKRRDSAT SCSLDFGHLR NKSGCHVELL 

        70         80         90        100        110        120 
FLRYISDWDL DPGRCYRVTW FTSWSPCYDC ARHVAEFLRW NPNLSLRIFT ARLYFCEDRK 

       130        140        150        160        170        180 
AEPEGLRRLH RAGVQIGIMT FKDYFYCWNT FVENRERTFK AWEGLHENSV RLTRQLRRIL 

       190 
LPLYEVDDLR DAFRMLGF 

« Hide

References

[1]"Specific expression of activation-induced cytidine deaminase (AID), a novel member of the RNA-editing deaminase family in germinal center B cells."
Muramatsu M., Sankaranand V.S., Anant S., Sugai M., Kinoshita K., Davidson N.O., Honjo T.
J. Biol. Chem. 274:18470-18476(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Transcription-targeted DNA deamination by the AID antibody diversification enzyme."
Chaudhuri J., Tian M., Khuong C., Chua K., Pinaud E., Alt F.W.
Nature 422:726-730(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF HIS-56 AND GLU-58.
[3]"Histone chaperone Spt6 is required for class switch recombination but not somatic hypermutation."
Okazaki I.M., Okawa K., Kobayashi M., Yoshikawa K., Kawamoto S., Nagaoka H., Shinkura R., Kitawaki Y., Taniguchi H., Natsume T., Iemura S., Honjo T.
Proc. Natl. Acad. Sci. U.S.A. 108:7920-7925(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SUPT6H; TRIM28 AND NCL.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF132979 mRNA. Translation: AAD41793.1.
RefSeqNP_033775.1. NM_009645.2.
UniGeneMm.391503.

3D structure databases

ProteinModelPortalQ9WVE0.
SMRQ9WVE0. Positions 6-180.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid198040. 2 interactions.
DIPDIP-48714N.
IntActQ9WVE0. 53 interactions.
STRING10090.ENSMUSP00000040524.

PTM databases

PhosphoSiteQ9WVE0.

Proteomic databases

PRIDEQ9WVE0.

Protocols and materials databases

DNASU11628.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000043301; ENSMUSP00000040524; ENSMUSG00000040627.
GeneID11628.
KEGGmmu:11628.
UCSCuc009dpj.1. mouse.

Organism-specific databases

CTD57379.
MGIMGI:1342279. Aicda.

Phylogenomic databases

eggNOGNOG39659.
GeneTreeENSGT00530000062933.
HOGENOMHOG000033754.
HOVERGENHBG050434.
InParanoidQ9WVE0.
KOK10989.
OMAIMTFKDY.
OrthoDBEOG708W0R.
PhylomeDBQ9WVE0.
TreeFamTF331356.

Gene expression databases

ArrayExpressQ9WVE0.
BgeeQ9WVE0.
CleanExMM_AICDA.
GenevestigatorQ9WVE0.

Family and domain databases

InterProIPR016192. APOBEC/CMP_deaminase_Zn-bd.
IPR013158. APOBEC_N.
IPR016193. Cytidine_deaminase-like.
[Graphical view]
PfamPF08210. APOBEC_N. 1 hit.
[Graphical view]
SUPFAMSSF53927. SSF53927. 1 hit.
PROSITEPS00903. CYT_DCMP_DEAMINASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio279183.
PROQ9WVE0.
SOURCESearch...

Entry information

Entry nameAICDA_MOUSE
AccessionPrimary (citable) accession number: Q9WVE0
Entry history
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: November 1, 1999
Last modified: April 16, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot