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Q9WVE0

- AICDA_MOUSE

UniProt

Q9WVE0 - AICDA_MOUSE

Protein

Single-stranded DNA cytosine deaminase

Gene

Aicda

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Single-stranded DNA-specific cytidine deaminase. Involved in somatic hypermutation, gene conversion, and class-switch recombination in B-lymphocytes. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation.1 Publication

    Catalytic activityi

    Cytosine in single-stranded DNA + H2O = uracil in single-stranded DNA + NH3.

    Cofactori

    Zinc.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi56 – 561Zinc; catalyticBy similarity
    Active sitei58 – 581Proton donorBy similarity
    Metal bindingi87 – 871Zinc; catalyticBy similarity
    Metal bindingi90 – 901Zinc; catalyticBy similarity

    GO - Molecular functioni

    1. cytidine deaminase activity Source: UniProtKB
    2. protein binding Source: UniProtKB
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. cellular response to lipopolysaccharide Source: MGI
    2. cytidine deamination Source: UniProtKB
    3. DNA demethylation Source: UniProtKB
    4. isotype switching Source: UniProtKB
    5. mRNA processing Source: UniProtKB-KW
    6. negative regulation of methylation-dependent chromatin silencing Source: UniProtKB
    7. somatic hypermutation of immunoglobulin genes Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    mRNA processing

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Single-stranded DNA cytosine deaminase (EC:3.5.4.38)
    Alternative name(s):
    Activation-induced cytidine deaminase
    Cytidine aminohydrolase
    Gene namesi
    Name:Aicda
    Synonyms:Aid
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:1342279. Aicda.

    Subcellular locationi

    Nucleus By similarity. Cytoplasm By similarity
    Note: Predominantly cytoplasmic but shuttles between the nucleus and the cytoplasm.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. exosome (RNase complex) Source: Ensembl
    3. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi56 – 561H → R: Loss of the cytidine deaminase activity; when associated with Q-58. 1 Publication
    Mutagenesisi58 – 581E → Q: Loss of the cytidine deaminase activity; when associated with R-56. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 198198Single-stranded DNA cytosine deaminasePRO_0000171688Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei27 – 271Phosphothreonine; by PKABy similarity
    Modified residuei38 – 381Phosphoserine; by PKABy similarity

    Post-translational modificationi

    Ser-38 is the major site whereas Thr-27 is the minor site of phosphorylation. Phosphorylation regulates its class-switch recombination activity By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ9WVE0.

    PTM databases

    PhosphoSiteiQ9WVE0.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9WVE0.
    BgeeiQ9WVE0.
    CleanExiMM_AICDA.
    GenevestigatoriQ9WVE0.

    Interactioni

    Subunit structurei

    Interacts with CTNNBL1; the interaction is important for the immunoglobulin switch activity of AICDA. Interacts (via its NLS) with KPNA1. Interacts with PKA/PRKACA and PRKAR1A/PKR1 By similarity. Interacts with SUPT6H, TRIM28 and NCL.By similarity1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    GADD45AP245223EBI-3835567,EBI-448167From a different organism.
    Ptbp2Q91Z314EBI-3835567,EBI-647632
    Top2bQ645113EBI-3835567,EBI-2325586

    Protein-protein interaction databases

    BioGridi198040. 2 interactions.
    DIPiDIP-48714N.
    IntActiQ9WVE0. 53 interactions.
    STRINGi10090.ENSMUSP00000040524.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9WVE0.
    SMRiQ9WVE0. Positions 6-180.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 3030Nuclear localization signalBy similarityAdd
    BLAST
    Regioni2 – 2625Interaction with SUPT6HBy similarityAdd
    BLAST
    Regioni39 – 424Important for interaction with CTNNBL1By similarity
    Regioni183 – 19816Nuclear export signalBy similarityAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG39659.
    GeneTreeiENSGT00530000062933.
    HOGENOMiHOG000033754.
    HOVERGENiHBG050434.
    InParanoidiQ9WVE0.
    KOiK10989.
    OMAiIMTFKDY.
    OrthoDBiEOG708W0R.
    PhylomeDBiQ9WVE0.
    TreeFamiTF331356.

    Family and domain databases

    InterProiIPR016192. APOBEC/CMP_deaminase_Zn-bd.
    IPR013158. APOBEC_N.
    IPR016193. Cytidine_deaminase-like.
    [Graphical view]
    PfamiPF08210. APOBEC_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF53927. SSF53927. 1 hit.
    PROSITEiPS00903. CYT_DCMP_DEAMINASES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9WVE0-1 [UniParc]FASTAAdd to Basket

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    MDSLLMKQKK FLYHFKNVRW AKGRHETYLC YVVKRRDSAT SCSLDFGHLR    50
    NKSGCHVELL FLRYISDWDL DPGRCYRVTW FTSWSPCYDC ARHVAEFLRW 100
    NPNLSLRIFT ARLYFCEDRK AEPEGLRRLH RAGVQIGIMT FKDYFYCWNT 150
    FVENRERTFK AWEGLHENSV RLTRQLRRIL LPLYEVDDLR DAFRMLGF 198
    Length:198
    Mass (Da):24,031
    Last modified:November 1, 1999 - v1
    Checksum:i18A3BA10CA54BEB2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF132979 mRNA. Translation: AAD41793.1.
    CCDSiCCDS20497.1.
    RefSeqiNP_033775.1. NM_009645.2.
    UniGeneiMm.391503.

    Genome annotation databases

    EnsembliENSMUST00000043301; ENSMUSP00000040524; ENSMUSG00000040627.
    GeneIDi11628.
    KEGGimmu:11628.
    UCSCiuc009dpj.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF132979 mRNA. Translation: AAD41793.1 .
    CCDSi CCDS20497.1.
    RefSeqi NP_033775.1. NM_009645.2.
    UniGenei Mm.391503.

    3D structure databases

    ProteinModelPortali Q9WVE0.
    SMRi Q9WVE0. Positions 6-180.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198040. 2 interactions.
    DIPi DIP-48714N.
    IntActi Q9WVE0. 53 interactions.
    STRINGi 10090.ENSMUSP00000040524.

    PTM databases

    PhosphoSitei Q9WVE0.

    Proteomic databases

    PRIDEi Q9WVE0.

    Protocols and materials databases

    DNASUi 11628.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000043301 ; ENSMUSP00000040524 ; ENSMUSG00000040627 .
    GeneIDi 11628.
    KEGGi mmu:11628.
    UCSCi uc009dpj.1. mouse.

    Organism-specific databases

    CTDi 57379.
    MGIi MGI:1342279. Aicda.

    Phylogenomic databases

    eggNOGi NOG39659.
    GeneTreei ENSGT00530000062933.
    HOGENOMi HOG000033754.
    HOVERGENi HBG050434.
    InParanoidi Q9WVE0.
    KOi K10989.
    OMAi IMTFKDY.
    OrthoDBi EOG708W0R.
    PhylomeDBi Q9WVE0.
    TreeFami TF331356.

    Miscellaneous databases

    NextBioi 279183.
    PROi Q9WVE0.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9WVE0.
    Bgeei Q9WVE0.
    CleanExi MM_AICDA.
    Genevestigatori Q9WVE0.

    Family and domain databases

    InterProi IPR016192. APOBEC/CMP_deaminase_Zn-bd.
    IPR013158. APOBEC_N.
    IPR016193. Cytidine_deaminase-like.
    [Graphical view ]
    Pfami PF08210. APOBEC_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53927. SSF53927. 1 hit.
    PROSITEi PS00903. CYT_DCMP_DEAMINASES. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Specific expression of activation-induced cytidine deaminase (AID), a novel member of the RNA-editing deaminase family in germinal center B cells."
      Muramatsu M., Sankaranand V.S., Anant S., Sugai M., Kinoshita K., Davidson N.O., Honjo T.
      J. Biol. Chem. 274:18470-18476(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Transcription-targeted DNA deamination by the AID antibody diversification enzyme."
      Chaudhuri J., Tian M., Khuong C., Chua K., Pinaud E., Alt F.W.
      Nature 422:726-730(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF HIS-56 AND GLU-58.
    3. Cited for: INTERACTION WITH SUPT6H; TRIM28 AND NCL.

    Entry informationi

    Entry nameiAICDA_MOUSE
    AccessioniPrimary (citable) accession number: Q9WVE0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 19, 2002
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3