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Q9WVE0

- AICDA_MOUSE

UniProt

Q9WVE0 - AICDA_MOUSE

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Protein

Single-stranded DNA cytosine deaminase

Gene

Aicda

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Single-stranded DNA-specific cytidine deaminase. Involved in somatic hypermutation, gene conversion, and class-switch recombination in B-lymphocytes. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation.1 Publication

Catalytic activityi

Cytosine in single-stranded DNA + H2O = uracil in single-stranded DNA + NH3.

Cofactori

Zn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi56 – 561Zinc; catalyticBy similarity
Active sitei58 – 581Proton donorBy similarity
Metal bindingi87 – 871Zinc; catalyticBy similarity
Metal bindingi90 – 901Zinc; catalyticBy similarity

GO - Molecular functioni

  1. cytidine deaminase activity Source: UniProtKB
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. cellular response to lipopolysaccharide Source: MGI
  2. cytidine deamination Source: UniProtKB
  3. DNA demethylation Source: UniProtKB
  4. isotype switching Source: UniProtKB
  5. mRNA processing Source: UniProtKB-KW
  6. negative regulation of methylation-dependent chromatin silencing Source: UniProtKB
  7. somatic hypermutation of immunoglobulin genes Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Single-stranded DNA cytosine deaminase (EC:3.5.4.38)
Alternative name(s):
Activation-induced cytidine deaminase
Cytidine aminohydrolase
Gene namesi
Name:Aicda
Synonyms:Aid
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:1342279. Aicda.

Subcellular locationi

Nucleus By similarity. Cytoplasm By similarity
Note: Predominantly cytoplasmic but shuttles between the nucleus and the cytoplasm.By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. exosome (RNase complex) Source: Ensembl
  3. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi56 – 561H → R: Loss of the cytidine deaminase activity; when associated with Q-58. 1 Publication
Mutagenesisi58 – 581E → Q: Loss of the cytidine deaminase activity; when associated with R-56. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 198198Single-stranded DNA cytosine deaminasePRO_0000171688Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei27 – 271Phosphothreonine; by PKABy similarity
Modified residuei38 – 381Phosphoserine; by PKABy similarity

Post-translational modificationi

Ser-38 is the major site whereas Thr-27 is the minor site of phosphorylation. Phosphorylation regulates its class-switch recombination activity (By similarity).By similarity
Probably monoubiquitinated on several residues by RNF126.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiQ9WVE0.

PTM databases

PhosphoSiteiQ9WVE0.

Expressioni

Gene expression databases

BgeeiQ9WVE0.
CleanExiMM_AICDA.
ExpressionAtlasiQ9WVE0. baseline and differential.
GenevestigatoriQ9WVE0.

Interactioni

Subunit structurei

Interacts with CTNNBL1; the interaction is important for the immunoglobulin switch activity of AICDA. Interacts (via its NLS) with KPNA1. Interacts with PKA/PRKACA and PRKAR1A/PKR1 (By similarity). Interacts with SUPT6H, TRIM28 and NCL.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
GADD45AP245223EBI-3835567,EBI-448167From a different organism.
Ptbp2Q91Z314EBI-3835567,EBI-647632
Top2bQ645113EBI-3835567,EBI-2325586

Protein-protein interaction databases

BioGridi198040. 2 interactions.
DIPiDIP-48714N.
IntActiQ9WVE0. 53 interactions.
STRINGi10090.ENSMUSP00000040524.

Structurei

3D structure databases

ProteinModelPortaliQ9WVE0.
SMRiQ9WVE0. Positions 6-180.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 3030Nuclear localization signalBy similarityAdd
BLAST
Regioni2 – 2625Interaction with SUPT6HBy similarityAdd
BLAST
Regioni39 – 424Important for interaction with CTNNBL1By similarity
Regioni88 – 11629Required for interaction with RNF126By similarityAdd
BLAST
Regioni183 – 19816Nuclear export signalBy similarityAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG39659.
GeneTreeiENSGT00530000062933.
HOGENOMiHOG000033754.
HOVERGENiHBG050434.
InParanoidiQ9WVE0.
KOiK10989.
OMAiIMTFKDY.
OrthoDBiEOG708W0R.
PhylomeDBiQ9WVE0.
TreeFamiTF331356.

Family and domain databases

InterProiIPR016192. APOBEC/CMP_deaminase_Zn-bd.
IPR013158. APOBEC_N.
IPR016193. Cytidine_deaminase-like.
[Graphical view]
PfamiPF08210. APOBEC_N. 1 hit.
[Graphical view]
SUPFAMiSSF53927. SSF53927. 1 hit.
PROSITEiPS00903. CYT_DCMP_DEAMINASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WVE0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDSLLMKQKK FLYHFKNVRW AKGRHETYLC YVVKRRDSAT SCSLDFGHLR
60 70 80 90 100
NKSGCHVELL FLRYISDWDL DPGRCYRVTW FTSWSPCYDC ARHVAEFLRW
110 120 130 140 150
NPNLSLRIFT ARLYFCEDRK AEPEGLRRLH RAGVQIGIMT FKDYFYCWNT
160 170 180 190
FVENRERTFK AWEGLHENSV RLTRQLRRIL LPLYEVDDLR DAFRMLGF
Length:198
Mass (Da):24,031
Last modified:November 1, 1999 - v1
Checksum:i18A3BA10CA54BEB2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF132979 mRNA. Translation: AAD41793.1.
CCDSiCCDS20497.1.
RefSeqiNP_033775.1. NM_009645.2.
UniGeneiMm.391503.

Genome annotation databases

EnsembliENSMUST00000043301; ENSMUSP00000040524; ENSMUSG00000040627.
GeneIDi11628.
KEGGimmu:11628.
UCSCiuc009dpj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF132979 mRNA. Translation: AAD41793.1 .
CCDSi CCDS20497.1.
RefSeqi NP_033775.1. NM_009645.2.
UniGenei Mm.391503.

3D structure databases

ProteinModelPortali Q9WVE0.
SMRi Q9WVE0. Positions 6-180.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 198040. 2 interactions.
DIPi DIP-48714N.
IntActi Q9WVE0. 53 interactions.
STRINGi 10090.ENSMUSP00000040524.

PTM databases

PhosphoSitei Q9WVE0.

Proteomic databases

PRIDEi Q9WVE0.

Protocols and materials databases

DNASUi 11628.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000043301 ; ENSMUSP00000040524 ; ENSMUSG00000040627 .
GeneIDi 11628.
KEGGi mmu:11628.
UCSCi uc009dpj.1. mouse.

Organism-specific databases

CTDi 57379.
MGIi MGI:1342279. Aicda.

Phylogenomic databases

eggNOGi NOG39659.
GeneTreei ENSGT00530000062933.
HOGENOMi HOG000033754.
HOVERGENi HBG050434.
InParanoidi Q9WVE0.
KOi K10989.
OMAi IMTFKDY.
OrthoDBi EOG708W0R.
PhylomeDBi Q9WVE0.
TreeFami TF331356.

Miscellaneous databases

NextBioi 279183.
PROi Q9WVE0.
SOURCEi Search...

Gene expression databases

Bgeei Q9WVE0.
CleanExi MM_AICDA.
ExpressionAtlasi Q9WVE0. baseline and differential.
Genevestigatori Q9WVE0.

Family and domain databases

InterProi IPR016192. APOBEC/CMP_deaminase_Zn-bd.
IPR013158. APOBEC_N.
IPR016193. Cytidine_deaminase-like.
[Graphical view ]
Pfami PF08210. APOBEC_N. 1 hit.
[Graphical view ]
SUPFAMi SSF53927. SSF53927. 1 hit.
PROSITEi PS00903. CYT_DCMP_DEAMINASES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Specific expression of activation-induced cytidine deaminase (AID), a novel member of the RNA-editing deaminase family in germinal center B cells."
    Muramatsu M., Sankaranand V.S., Anant S., Sugai M., Kinoshita K., Davidson N.O., Honjo T.
    J. Biol. Chem. 274:18470-18476(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Transcription-targeted DNA deamination by the AID antibody diversification enzyme."
    Chaudhuri J., Tian M., Khuong C., Chua K., Pinaud E., Alt F.W.
    Nature 422:726-730(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF HIS-56 AND GLU-58.
  3. Cited for: INTERACTION WITH SUPT6H; TRIM28 AND NCL.

Entry informationi

Entry nameiAICDA_MOUSE
AccessioniPrimary (citable) accession number: Q9WVE0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: November 1, 1999
Last modified: November 26, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3