Q9WVE0 (AICDA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Activation-induced cytidine deaminase
EC=3.5.4.5

Alternative name(s):
Cytidine aminohydrolase

Gene names

Name:	Aicda
Synonyms:	Aid

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Single-stranded DNA-specific cytidine deaminase. Involved in somatic hypermutation, gene conversion, and class-switch recombination in B-lymphocytes. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Ref.2
Catalytic activity	Cytidine + H2O = uridine + NH3.
Cofactor	Zinc By similarity.
Sequence similarities	Belongs to the cytidine and deoxycytidylate deaminase family.

Ontologies

Keywords
Biological process	mRNA processing
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA demethylation Inferred from sequence or structural similarity. Source: UniProtKB isotype switching Inferred from mutant phenotype. Source: MGI mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of methylation-dependent chromatin silencing Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	nucleus Inferred by curator. Source: MGI
Molecular function	cytidine deaminase activity Inferred from mutant phenotype Ref.2. Source: UniProtKB protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Ptbp2	Q91Z31	4	EBI-3835567,EBI-647632
Top2b	Q64511	3	EBI-3835567,EBI-2325586

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 198	198	Activation-induced cytidine deaminase		PRO_0000171688
Sites
	Active site	58	1	Proton donor By similarity
	Metal binding	56	1	Zinc; catalytic By similarity
	Metal binding	87	1	Zinc; catalytic By similarity
	Metal binding	90	1	Zinc; catalytic By similarity
Experimental info
	Mutagenesis	56	1	H → R: Loss of the cytidine deaminase activity; when associated with Q-58. Ref.2
	Mutagenesis	58	1	E → Q: Loss of the cytidine deaminase activity; when associated with R-56. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9WVE0 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 18A3BA10CA54BEB2	FASTA	198	24,031
	10 20 30 40 50 60 MDSLLMKQKK FLYHFKNVRW AKGRHETYLC YVVKRRDSAT SCSLDFGHLR NKSGCHVELL 70 80 90 100 110 120 FLRYISDWDL DPGRCYRVTW FTSWSPCYDC ARHVAEFLRW NPNLSLRIFT ARLYFCEDRK 130 140 150 160 170 180 AEPEGLRRLH RAGVQIGIMT FKDYFYCWNT FVENRERTFK AWEGLHENSV RLTRQLRRIL 190 LPLYEVDDLR DAFRMLGF

References

[1]	"Specific expression of activation-induced cytidine deaminase (AID), a novel member of the RNA-editing deaminase family in germinal center B cells." Muramatsu M., Sankaranand V.S., Anant S., Sugai M., Kinoshita K., Davidson N.O., Honjo T. J. Biol. Chem. 274:18470-18476(1999) [PubMed: 10373455] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Transcription-targeted DNA deamination by the AID antibody diversification enzyme." Chaudhuri J., Tian M., Khuong C., Chua K., Pinaud E., Alt F.W. Nature 422:726-730(2003) [PubMed: 12692563] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF HIS-56 AND GLU-58.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF132979 mRNA. Translation: AAD41793.1.
IPI	IPI00125873.
RefSeq	NP_033775.1. NM_009645.2.
UniGene	Mm.391503.
3D structure databases
ProteinModelPortal	Q9WVE0.
SMR	Q9WVE0. Positions 3-182.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48714N.
IntAct	Q9WVE0. 52 interactions.
STRING	Q9WVE0.
PTM databases
PhosphoSite	Q9WVE0.
Proteomic databases
PRIDE	Q9WVE0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000043301; ENSMUSP00000040524; ENSMUSG00000040627.
GeneID	11628.
KEGG	mmu:11628.
Organism-specific databases
CTD	57379.
MGI	MGI:1342279. Aicda.
Phylogenomic databases
GeneTree	ENSGT00530000062933.
HOGENOM	HBG716052.
HOVERGEN	HBG050434.
InParanoid	Q9WVE0.
OMA	CWNTFVE.
OrthoDB	EOG4GQQ5W.
PhylomeDB	Q9WVE0.
Gene expression databases
ArrayExpress	Q9WVE0.
Bgee	Q9WVE0.
CleanEx	MM_AICDA.
Genevestigator	Q9WVE0.
GermOnline	ENSMUSG00000040627. Mus musculus.
Family and domain databases
InterPro	IPR016192. APOBEC/CMP_deaminase_Zn-bd. IPR007904. APOBEC_C. IPR013158. APOBEC_N. IPR016193. Cytidine_deaminase-like. [Graphical view]
KO	K10989.
Pfam	PF05240. APOBEC_C. 1 hit. PF08210. APOBEC_N. 1 hit. [Graphical view]
SUPFAM	SSF53927. Cytidine_deaminase-like. 1 hit.
PROSITE	PS00903. CYT_DCMP_DEAMINASES. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	279183.
SOURCE	Search...

Entry information

Entry name

AICDA_MOUSE

Accession

Primary (citable) accession number: Q9WVE0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2002
Last sequence update:	November 1, 1999
Last modified:	January 25, 2012
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program