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Protein

Single-stranded DNA cytosine deaminase

Gene

Aicda

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Single-stranded DNA-specific cytidine deaminase. Involved in somatic hypermutation, gene conversion, and class-switch recombination in B-lymphocytes. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation.1 Publication

Catalytic activityi

Cytosine in single-stranded DNA + H2O = uracil in single-stranded DNA + NH3.

Cofactori

Zn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi56Zinc; catalyticBy similarity1
Active sitei58Proton donorBy similarity1
Metal bindingi87Zinc; catalyticBy similarity1
Metal bindingi90Zinc; catalyticBy similarity1

GO - Molecular functioni

  • cytidine deaminase activity Source: UniProtKB
  • ubiquitin protein ligase binding Source: MGI
  • zinc ion binding Source: InterPro

GO - Biological processi

  • cellular response to lipopolysaccharide Source: MGI
  • cytidine deamination Source: UniProtKB
  • DNA demethylation Source: UniProtKB
  • isotype switching Source: UniProtKB
  • mRNA processing Source: UniProtKB-KW
  • negative regulation of methylation-dependent chromatin silencing Source: UniProtKB
  • somatic diversification of immunoglobulins Source: MGI
  • somatic hypermutation of immunoglobulin genes Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

mRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.5.4.38. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Single-stranded DNA cytosine deaminase (EC:3.5.4.38)
Alternative name(s):
Activation-induced cytidine deaminase
Cytidine aminohydrolase
Gene namesi
Name:Aicda
Synonyms:Aid
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1342279. Aicda.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity

  • Note: Predominantly cytoplasmic but shuttles between the nucleus and the cytoplasm.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi56H → R: Loss of the cytidine deaminase activity; when associated with Q-58. 1 Publication1
Mutagenesisi58E → Q: Loss of the cytidine deaminase activity; when associated with R-56. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001716881 – 198Single-stranded DNA cytosine deaminaseAdd BLAST198

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei27Phosphothreonine; by PKABy similarity1
Modified residuei38Phosphoserine; by PKABy similarity1

Post-translational modificationi

Ser-38 is the major site whereas Thr-27 is the minor site of phosphorylation. Phosphorylation regulates its class-switch recombination activity (By similarity).By similarity
Probably monoubiquitinated on several residues by RNF126.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9WVE0.
PRIDEiQ9WVE0.

PTM databases

iPTMnetiQ9WVE0.
PhosphoSitePlusiQ9WVE0.

Expressioni

Gene expression databases

BgeeiENSMUSG00000040627.
CleanExiMM_AICDA.
ExpressionAtlasiQ9WVE0. baseline and differential.
GenevisibleiQ9WVE0. MM.

Interactioni

Subunit structurei

Interacts with CTNNBL1; the interaction is important for the immunoglobulin switch activity of AICDA. Interacts (via its NLS) with KPNA1. Interacts with PKA/PRKACA and PRKAR1A/PKR1 (By similarity). Interacts with SUPT6H, TRIM28 and NCL.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
GADD45AP245223EBI-3835567,EBI-448167From a different organism.
Ptbp2Q91Z314EBI-3835567,EBI-647632
Top2bQ645113EBI-3835567,EBI-2325586

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198040. 2 interactors.
DIPiDIP-48714N.
IntActiQ9WVE0. 53 interactors.
STRINGi10090.ENSMUSP00000040524.

Structurei

3D structure databases

ProteinModelPortaliQ9WVE0.
SMRiQ9WVE0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 129CMP/dCMP-type deaminasePROSITE-ProRule annotationAdd BLAST107

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 26Interaction with SUPT6HBy similarityAdd BLAST25
Regioni39 – 42Important for interaction with CTNNBL1By similarity4
Regioni88 – 116Required for interaction with RNF126By similarityAdd BLAST29

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1 – 30Nuclear localization signalBy similarityAdd BLAST30
Motifi183 – 198Nuclear export signalBy similarityAdd BLAST16

Sequence similaritiesi

Contains 1 CMP/dCMP-type deaminase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IFHW. Eukaryota.
ENOG4111HYQ. LUCA.
GeneTreeiENSGT00530000062933.
HOGENOMiHOG000033754.
HOVERGENiHBG050434.
InParanoidiQ9WVE0.
KOiK10989.
OMAiRNKNGCH.
OrthoDBiEOG091G0J2L.
PhylomeDBiQ9WVE0.
TreeFamiTF331356.

Family and domain databases

InterProiIPR016192. APOBEC/CMP_deaminase_Zn-bd.
IPR013158. APOBEC_N.
IPR002125. CMP_dCMP_Zn-bd.
IPR016193. Cytidine_deaminase-like.
[Graphical view]
PfamiPF08210. APOBEC_N. 1 hit.
[Graphical view]
SUPFAMiSSF53927. SSF53927. 1 hit.
PROSITEiPS00903. CYT_DCMP_DEAMINASES_1. 1 hit.
PS51747. CYT_DCMP_DEAMINASES_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WVE0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSLLMKQKK FLYHFKNVRW AKGRHETYLC YVVKRRDSAT SCSLDFGHLR
60 70 80 90 100
NKSGCHVELL FLRYISDWDL DPGRCYRVTW FTSWSPCYDC ARHVAEFLRW
110 120 130 140 150
NPNLSLRIFT ARLYFCEDRK AEPEGLRRLH RAGVQIGIMT FKDYFYCWNT
160 170 180 190
FVENRERTFK AWEGLHENSV RLTRQLRRIL LPLYEVDDLR DAFRMLGF
Length:198
Mass (Da):24,031
Last modified:November 1, 1999 - v1
Checksum:i18A3BA10CA54BEB2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF132979 mRNA. Translation: AAD41793.1.
CCDSiCCDS20497.1.
RefSeqiNP_033775.1. NM_009645.2.
UniGeneiMm.391503.

Genome annotation databases

EnsembliENSMUST00000043301; ENSMUSP00000040524; ENSMUSG00000040627.
GeneIDi11628.
KEGGimmu:11628.
UCSCiuc009dpj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF132979 mRNA. Translation: AAD41793.1.
CCDSiCCDS20497.1.
RefSeqiNP_033775.1. NM_009645.2.
UniGeneiMm.391503.

3D structure databases

ProteinModelPortaliQ9WVE0.
SMRiQ9WVE0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198040. 2 interactors.
DIPiDIP-48714N.
IntActiQ9WVE0. 53 interactors.
STRINGi10090.ENSMUSP00000040524.

PTM databases

iPTMnetiQ9WVE0.
PhosphoSitePlusiQ9WVE0.

Proteomic databases

PaxDbiQ9WVE0.
PRIDEiQ9WVE0.

Protocols and materials databases

DNASUi11628.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000043301; ENSMUSP00000040524; ENSMUSG00000040627.
GeneIDi11628.
KEGGimmu:11628.
UCSCiuc009dpj.1. mouse.

Organism-specific databases

CTDi57379.
MGIiMGI:1342279. Aicda.

Phylogenomic databases

eggNOGiENOG410IFHW. Eukaryota.
ENOG4111HYQ. LUCA.
GeneTreeiENSGT00530000062933.
HOGENOMiHOG000033754.
HOVERGENiHBG050434.
InParanoidiQ9WVE0.
KOiK10989.
OMAiRNKNGCH.
OrthoDBiEOG091G0J2L.
PhylomeDBiQ9WVE0.
TreeFamiTF331356.

Enzyme and pathway databases

BRENDAi3.5.4.38. 3474.

Miscellaneous databases

PROiQ9WVE0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040627.
CleanExiMM_AICDA.
ExpressionAtlasiQ9WVE0. baseline and differential.
GenevisibleiQ9WVE0. MM.

Family and domain databases

InterProiIPR016192. APOBEC/CMP_deaminase_Zn-bd.
IPR013158. APOBEC_N.
IPR002125. CMP_dCMP_Zn-bd.
IPR016193. Cytidine_deaminase-like.
[Graphical view]
PfamiPF08210. APOBEC_N. 1 hit.
[Graphical view]
SUPFAMiSSF53927. SSF53927. 1 hit.
PROSITEiPS00903. CYT_DCMP_DEAMINASES_1. 1 hit.
PS51747. CYT_DCMP_DEAMINASES_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAICDA_MOUSE
AccessioniPrimary (citable) accession number: Q9WVE0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: November 1, 1999
Last modified: November 2, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.