Activation-induced cytidine deaminase

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Q9WVE0 (AICDA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 91. History...

Clusters with 100%, 90%, 50% identity |

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Activation-induced cytidine deaminase
EC=3.5.4.5

Alternative name(s):
Cytidine aminohydrolase

Gene names

Name:	Aicda
Synonyms:	Aid

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Single-stranded DNA-specific cytidine deaminase. Involved in somatic hypermutation, gene conversion, and class-switch recombination in B-lymphocytes. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Ref.2
Catalytic activity	Cytidine + H₂O = uridine + NH₃.
Cofactor	Zinc By similarity.
Subunit structure	Interacts with CTNNBL1; the interaction is important for the immunoglobulin switch activity of AICDA. Interacts (via its NLS) with KPNA1. Interacts with PKA/PRKACA and PRKAR1A/PKR1 By similarity.
Subcellular location	Nucleus By similarity. Cytoplasm By similarity. Note: Predominantly cytoplasmic but shuttles between the nucleus and the cytoplasm By similarity.
Post-translational modification	Ser-38 is the major site whereas Thr-27 is the minor site of phosphorylation. Phosphorylation regulates its class-switch recombination activity By similarity.
Sequence similarities	Belongs to the cytidine and deoxycytidylate deaminase family.

Ontologies

Keywords
Biological process	mRNA processing
Cellular component	Cytoplasm Nucleus
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA demethylation Inferred from sequence or structural similarity. Source: UniProtKB cellular response to lipopolysaccharide Inferred from mutant phenotype PubMed 18762567. Source: MGI isotype switching Inferred from mutant phenotype PubMed 17713479. Source: MGI mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of methylation-dependent chromatin silencing Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB exosome (RNase complex) Inferred from electronic annotation. Source: Compara nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	cytidine deaminase activity Inferred from mutant phenotype Ref.2. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Ptbp2	Q91Z31	4	EBI-3835567,EBI-647632
Top2b	Q64511	3	EBI-3835567,EBI-2325586

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 198

198

Activation-induced cytidine deaminase

PRO_0000171688

Regions

Region

1 – 30

Nuclear localization signal By similarity

Region

39 – 42

Important for interaction with CTNNBL1 By similarity

Region

183 – 198

Nuclear export signal By similarity

Sites

Active site

Proton donor By similarity

Metal binding

Zinc; catalytic By similarity

Metal binding

Zinc; catalytic By similarity

Metal binding

Zinc; catalytic By similarity

Amino acid modifications

Modified residue

Phosphothreonine; by PKA By similarity

Modified residue

Phosphoserine; by PKA By similarity

Experimental info

Mutagenesis

H → R: Loss of the cytidine deaminase activity; when associated with Q-58. Ref.2

Mutagenesis

E → Q: Loss of the cytidine deaminase activity; when associated with R-56. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

Q9WVE0 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 18A3BA10CA54BEB2
Show »

FASTA

198

24,031

        10         20         30         40         50         60 
MDSLLMKQKK FLYHFKNVRW AKGRHETYLC YVVKRRDSAT SCSLDFGHLR NKSGCHVELL 

        70         80         90        100        110        120 
FLRYISDWDL DPGRCYRVTW FTSWSPCYDC ARHVAEFLRW NPNLSLRIFT ARLYFCEDRK 

       130        140        150        160        170        180 
AEPEGLRRLH RAGVQIGIMT FKDYFYCWNT FVENRERTFK AWEGLHENSV RLTRQLRRIL 

       190 
LPLYEVDDLR DAFRMLGF

« Hide

References

[1]	"Specific expression of activation-induced cytidine deaminase (AID), a novel member of the RNA-editing deaminase family in germinal center B cells." Muramatsu M., Sankaranand V.S., Anant S., Sugai M., Kinoshita K., Davidson N.O., Honjo T. J. Biol. Chem. 274:18470-18476(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Transcription-targeted DNA deamination by the AID antibody diversification enzyme." Chaudhuri J., Tian M., Khuong C., Chua K., Pinaud E., Alt F.W. Nature 422:726-730(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF HIS-56 AND GLU-58.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF132979 mRNA. Translation: AAD41793.1.
IPI	IPI00125873.
RefSeq	NP_033775.1. NM_009645.2.
UniGene	Mm.391503.
3D structure databases
ProteinModelPortal	Q9WVE0.
SMR	Q9WVE0. Positions 6-180.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48714N.
IntAct	Q9WVE0. 52 interactions.
STRING	10090.ENSMUSP00000040524.
PTM databases
PhosphoSite	Q9WVE0.
Proteomic databases
PRIDE	Q9WVE0.
Protocols and materials databases
DNASU	11628.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000043301; ENSMUSP00000040524; ENSMUSG00000040627.
GeneID	11628.
KEGG	mmu:11628.
Organism-specific databases
CTD	57379.
MGI	MGI:1342279. Aicda.
Phylogenomic databases
eggNOG	NOG39659.
GeneTree	ENSGT00530000062933.
HOGENOM	HOG000033754.
HOVERGEN	HBG050434.
InParanoid	Q9WVE0.
KO	K10989.
OMA	IMTFKDY.
OrthoDB	EOG4GQQ5W.
Gene expression databases
ArrayExpress	Q9WVE0.
Bgee	Q9WVE0.
CleanEx	MM_AICDA.
Genevestigator	Q9WVE0.
GermOnline	ENSMUSG00000040627. Mus musculus.
Family and domain databases
InterPro	IPR016192. APOBEC/CMP_deaminase_Zn-bd. IPR013158. APOBEC_N. IPR016193. Cytidine_deaminase-like. [Graphical view]
Pfam	PF08210. APOBEC_N. 1 hit. [Graphical view]
SUPFAM	SSF53927. Cytidine_deaminase-like. 1 hit.
PROSITE	PS00903. CYT_DCMP_DEAMINASES. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	279183.
SOURCE	Search...

Entry information

Entry name

AICDA_MOUSE

Accession

Primary (citable) accession number: Q9WVE0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2002
Last sequence update:	November 1, 1999
Last modified:	May 1, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents