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Q9WVD4

- CLCN5_MOUSE

UniProt

Q9WVD4 - CLCN5_MOUSE

Protein

H(+)/Cl(-) exchange transporter 5

Gene

Clcn5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons. Important for normal acidification of the endosome lumen. May play an important role in renal tubular function By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei168 – 1681ChlorideBy similarity
    Sitei211 – 2111Mediates proton transfer from the outer aqueous phase to the interior of the protein; involved in linking H(+) and Cl(-) transportBy similarity
    Sitei268 – 2681Mediates proton transfer from the protein to the inner aqueous phaseBy similarity
    Binding sitei455 – 4551Chloride; via amide nitrogenBy similarity
    Binding sitei558 – 5581ChlorideBy similarity
    Binding sitei596 – 5961ATP; via amide nitrogen and carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi617 – 6193ATPBy similarity
    Nucleotide bindingi724 – 7274ATPBy similarity

    GO - Molecular functioni

    1. antiporter activity Source: UniProtKB-KW
    2. ATP binding Source: UniProtKB-KW
    3. voltage-gated chloride channel activity Source: InterPro

    GO - Biological processi

    1. endocytosis Source: MGI

    Keywords - Biological processi

    Antiport, Ion transport, Transport

    Keywords - Ligandi

    ATP-binding, Chloride, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_196640. Stimuli-sensing channels.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    H(+)/Cl(-) exchange transporter 5
    Alternative name(s):
    Chloride channel protein 5
    Short name:
    ClC-5
    Chloride transporter ClC-5
    Gene namesi
    Name:Clcn5
    Synonyms:Clc5
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:99486. Clcn5.

    Subcellular locationi

    Golgi apparatus membrane By similarity; Multi-pass membrane protein By similarity. Endosome membrane By similarity; Multi-pass membrane protein By similarity. Cell membrane By similarity; Multi-pass membrane protein By similarity

    GO - Cellular componenti

    1. apical part of cell Source: UniProtKB
    2. endosome membrane Source: UniProtKB-SubCell
    3. Golgi membrane Source: UniProtKB-SubCell
    4. integral component of membrane Source: UniProtKB-KW
    5. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Endosome, Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 746746H(+)/Cl(-) exchange transporter 5PRO_0000094447Add
    BLAST

    Post-translational modificationi

    Ubiquitinated by NEDD4L in the presence of albumin; which promotes endocytosis and proteasomal degradation.By similarity

    Keywords - PTMi

    Ubl conjugation

    Proteomic databases

    MaxQBiQ9WVD4.
    PaxDbiQ9WVD4.
    PRIDEiQ9WVD4.

    Expressioni

    Tissue specificityi

    Kidney specific.

    Gene expression databases

    ArrayExpressiQ9WVD4.
    BgeeiQ9WVD4.
    CleanExiMM_CLCN5.
    GenevestigatoriQ9WVD4.

    Interactioni

    Subunit structurei

    Interacts with NEDD4 and NEDD4L.By similarity

    Protein-protein interaction databases

    IntActiQ9WVD4. 1 interaction.
    MINTiMINT-4998506.
    STRINGi10090.ENSMUSP00000111412.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9WVD4.
    SMRiQ9WVD4. Positions 132-737.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 5454CytoplasmicBy similarityAdd
    BLAST
    Topological domaini553 – 746194CytoplasmicBy similarityAdd
    BLAST

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei170 – 1778HelicalBy similarity
    Intramembranei242 – 25413HelicalBy similarityAdd
    BLAST
    Intramembranei258 – 2669HelicalBy similarity
    Intramembranei500 – 51415HelicalBy similarityAdd
    BLAST
    Intramembranei515 – 5173Note=Loop between two helicesBy similarity
    Intramembranei518 – 52912HelicalBy similarityAdd
    BLAST
    Intramembranei530 – 5345Note=Loop between two helicesBy similarity

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei55 – 9238HelicalBy similarityAdd
    BLAST
    Transmembranei138 – 16124HelicalBy similarityAdd
    BLAST
    Transmembranei186 – 20520HelicalBy similarityAdd
    BLAST
    Transmembranei211 – 23020HelicalBy similarityAdd
    BLAST
    Transmembranei278 – 29619HelicalBy similarityAdd
    BLAST
    Transmembranei319 – 34426HelicalBy similarityAdd
    BLAST
    Transmembranei352 – 37221HelicalBy similarityAdd
    BLAST
    Transmembranei428 – 44821HelicalBy similarityAdd
    BLAST
    Transmembranei453 – 47220HelicalBy similarityAdd
    BLAST
    Transmembranei535 – 55218HelicalBy similarityAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini586 – 65065CBS 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini682 – 74261CBS 2PROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi167 – 1715Selectivity filter part_1By similarity
    Motifi209 – 2135Selectivity filter part_2By similarity
    Motifi453 – 4575Selectivity filter part_3By similarity

    Sequence similaritiesi

    Contains 2 CBS domains.PROSITE-ProRule annotation

    Keywords - Domaini

    CBS domain, Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0038.
    GeneTreeiENSGT00620000087693.
    HOGENOMiHOG000164493.
    HOVERGENiHBG050984.
    InParanoidiB1AXN0.
    KOiK05012.
    OrthoDBiEOG7MWGXT.
    PhylomeDBiQ9WVD4.
    TreeFamiTF313867.

    Family and domain databases

    Gene3Di1.10.3080.10. 3 hits.
    InterProiIPR000644. CBS_dom.
    IPR014743. Cl-channel_core.
    IPR001807. Cl-channel_volt-gated.
    IPR002247. Cl_channel-5.
    [Graphical view]
    PfamiPF00571. CBS. 2 hits.
    PF00654. Voltage_CLC. 1 hit.
    [Graphical view]
    PRINTSiPR00762. CLCHANNEL.
    PR01116. CLCHANNEL5.
    SMARTiSM00116. CBS. 2 hits.
    [Graphical view]
    SUPFAMiSSF81340. SSF81340. 3 hits.
    PROSITEiPS51371. CBS. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9WVD4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDFLEEPIPG VGTYDDFNTI DWVREKSRDR DRHREITNKS KESTWALIHS    50
    VSDAFSGWLL MLLIGLLSGS LAGLIDISAH WMTDLKEGIC TGGFWFNHEH 100
    CCWNSEHVTF EHRDKCPEWN SWAQLIINTD QGAFAYIVNY FMYVLWALLF 150
    AFLAVSLVKA FAPYACGSGI PEIKTILSGF IIRGYLGKWT LVIKTITLVL 200
    AVSSGLSLGK EGPLVHVACC CGNILCHCFN KYRKNEAKRR EVLSAAAAAG 250
    VSVAFGAPIG GVLFSLEEVS YYFPLKTLWR SFFAALVAAF TLRSINPFGN 300
    SRLVLFYVEF HTPWHLFELV PFIVLGIFGG LWGALFIRTN IAWCRKRKTT 350
    QLGKYPVVEV LIVTAITAIL AFPNEYTRMS TSELISELFN DCGLLDSSKL 400
    CDYENHFNTS KGGELPDRPA GVGIYSAMWQ LALTLILKIV ITIFTFGMKI 450
    PSGLFIPSMA VGAIAGRLLG VGMEQLAYYH HDWGIFNSWC SQGADCITPG 500
    LYAMVGAAAC LGGVTRMTVS LVVIMFELTG GLEYIVPLMA AAMTSKWVAD 550
    ALGREGIYDA HIRLNGYPFL EAKEEFAHKT LAMDVMKPRR NDPLLTVLTQ 600
    DSMTVEDVET IISETTYSGF PVVVSRESQR LVGFVLRRDL IISIENARKK 650
    QDGVVSTSII YFTEHSPPMP PYTPPTLKLR NILDLSPFTV TDLTPMEIVV 700
    DIFRKLGLRQ CLVTHNGRLL GIITKKDVLK HIAQMANQDP DSILFN 746
    Length:746
    Mass (Da):83,101
    Last modified:November 1, 1999 - v1
    Checksum:iD8F3AE4FFC331A08
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF134117 mRNA. Translation: AAD28473.1.
    AL808124 Genomic DNA. Translation: CAM15287.1.
    CCDSiCCDS29963.1.
    RefSeqiNP_057900.3. NM_016691.4.
    UniGeneiMm.486257.

    Genome annotation databases

    EnsembliENSMUST00000004428; ENSMUSP00000004428; ENSMUSG00000004317.
    GeneIDi12728.
    KEGGimmu:12728.
    UCSCiuc009sle.3. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF134117 mRNA. Translation: AAD28473.1 .
    AL808124 Genomic DNA. Translation: CAM15287.1 .
    CCDSi CCDS29963.1.
    RefSeqi NP_057900.3. NM_016691.4.
    UniGenei Mm.486257.

    3D structure databases

    ProteinModelPortali Q9WVD4.
    SMRi Q9WVD4. Positions 132-737.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9WVD4. 1 interaction.
    MINTi MINT-4998506.
    STRINGi 10090.ENSMUSP00000111412.

    Proteomic databases

    MaxQBi Q9WVD4.
    PaxDbi Q9WVD4.
    PRIDEi Q9WVD4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000004428 ; ENSMUSP00000004428 ; ENSMUSG00000004317 .
    GeneIDi 12728.
    KEGGi mmu:12728.
    UCSCi uc009sle.3. mouse.

    Organism-specific databases

    CTDi 1184.
    MGIi MGI:99486. Clcn5.

    Phylogenomic databases

    eggNOGi COG0038.
    GeneTreei ENSGT00620000087693.
    HOGENOMi HOG000164493.
    HOVERGENi HBG050984.
    InParanoidi B1AXN0.
    KOi K05012.
    OrthoDBi EOG7MWGXT.
    PhylomeDBi Q9WVD4.
    TreeFami TF313867.

    Enzyme and pathway databases

    Reactomei REACT_196640. Stimuli-sensing channels.

    Miscellaneous databases

    ChiTaRSi CLCN5. mouse.
    NextBioi 282026.
    PROi Q9WVD4.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9WVD4.
    Bgeei Q9WVD4.
    CleanExi MM_CLCN5.
    Genevestigatori Q9WVD4.

    Family and domain databases

    Gene3Di 1.10.3080.10. 3 hits.
    InterProi IPR000644. CBS_dom.
    IPR014743. Cl-channel_core.
    IPR001807. Cl-channel_volt-gated.
    IPR002247. Cl_channel-5.
    [Graphical view ]
    Pfami PF00571. CBS. 2 hits.
    PF00654. Voltage_CLC. 1 hit.
    [Graphical view ]
    PRINTSi PR00762. CLCHANNEL.
    PR01116. CLCHANNEL5.
    SMARTi SM00116. CBS. 2 hits.
    [Graphical view ]
    SUPFAMi SSF81340. SSF81340. 3 hits.
    PROSITEi PS51371. CBS. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of novel promoter and enhancer elements of the mouse homologue of the Dent disease gene, CLCN5, implicated in X-linked hereditary nephrolithiasis."
      Tanaka K., Fisher S.E., Craig I.W.
      Genomics 58:281-292(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Kidney.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.

    Entry informationi

    Entry nameiCLCN5_MOUSE
    AccessioniPrimary (citable) accession number: Q9WVD4
    Secondary accession number(s): B1AXN0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons. The absence of conserved gating glutamate residues is typical for family members that function as channels By similarity.By similarity

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3