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Q9WVD4

- CLCN5_MOUSE

UniProt

Q9WVD4 - CLCN5_MOUSE

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Protein

H(+)/Cl(-) exchange transporter 5

Gene
Clcn5, Clc5
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons. Important for normal acidification of the endosome lumen. May play an important role in renal tubular function By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei168 – 1681Chloride By similarity
Sitei211 – 2111Mediates proton transfer from the outer aqueous phase to the interior of the protein; involved in linking H(+) and Cl(-) transport By similarity
Sitei268 – 2681Mediates proton transfer from the protein to the inner aqueous phase By similarity
Binding sitei455 – 4551Chloride; via amide nitrogen By similarity
Binding sitei558 – 5581Chloride By similarity
Binding sitei596 – 5961ATP; via amide nitrogen and carbonyl oxygen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi617 – 6193ATP By similarity
Nucleotide bindingi724 – 7274ATP By similarity

GO - Molecular functioni

  1. antiporter activity Source: UniProtKB-KW
  2. ATP binding Source: UniProtKB-KW
  3. voltage-gated chloride channel activity Source: InterPro

GO - Biological processi

  1. endocytosis Source: MGI
Complete GO annotation...

Keywords - Biological processi

Antiport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Chloride, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_196640. Stimuli-sensing channels.

Names & Taxonomyi

Protein namesi
Recommended name:
H(+)/Cl(-) exchange transporter 5
Alternative name(s):
Chloride channel protein 5
Short name:
ClC-5
Chloride transporter ClC-5
Gene namesi
Name:Clcn5
Synonyms:Clc5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:99486. Clcn5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5454Cytoplasmic By similarityAdd
BLAST
Transmembranei55 – 9238Helical; By similarityAdd
BLAST
Transmembranei138 – 16124Helical; By similarityAdd
BLAST
Intramembranei170 – 1778Helical; By similarity
Transmembranei186 – 20520Helical; By similarityAdd
BLAST
Transmembranei211 – 23020Helical; By similarityAdd
BLAST
Intramembranei242 – 25413Helical; By similarityAdd
BLAST
Intramembranei258 – 2669Helical; By similarity
Transmembranei278 – 29619Helical; By similarityAdd
BLAST
Transmembranei319 – 34426Helical; By similarityAdd
BLAST
Transmembranei352 – 37221Helical; By similarityAdd
BLAST
Transmembranei428 – 44821Helical; By similarityAdd
BLAST
Transmembranei453 – 47220Helical; By similarityAdd
BLAST
Intramembranei500 – 51415Helical; By similarityAdd
BLAST
Intramembranei515 – 5173Note=Loop between two helices; By similarity
Intramembranei518 – 52912Helical; By similarityAdd
BLAST
Intramembranei530 – 5345Note=Loop between two helices; By similarity
Transmembranei535 – 55218Helical; By similarityAdd
BLAST
Topological domaini553 – 746194Cytoplasmic By similarityAdd
BLAST

GO - Cellular componenti

  1. apical part of cell Source: UniProtKB
  2. endosome membrane Source: UniProtKB-SubCell
  3. Golgi membrane Source: UniProtKB-SubCell
  4. integral component of membrane Source: UniProtKB-KW
  5. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 746746H(+)/Cl(-) exchange transporter 5PRO_0000094447Add
BLAST

Post-translational modificationi

Ubiquitinated by NEDD4L in the presence of albumin; which promotes endocytosis and proteasomal degradation By similarity.

Keywords - PTMi

Ubl conjugation

Proteomic databases

MaxQBiQ9WVD4.
PaxDbiQ9WVD4.
PRIDEiQ9WVD4.

Expressioni

Tissue specificityi

Kidney specific.

Gene expression databases

ArrayExpressiQ9WVD4.
BgeeiQ9WVD4.
CleanExiMM_CLCN5.
GenevestigatoriQ9WVD4.

Interactioni

Subunit structurei

Interacts with NEDD4 and NEDD4L By similarity.

Protein-protein interaction databases

IntActiQ9WVD4. 1 interaction.
MINTiMINT-4998506.
STRINGi10090.ENSMUSP00000111412.

Structurei

3D structure databases

ProteinModelPortaliQ9WVD4.
SMRiQ9WVD4. Positions 132-737.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini586 – 65065CBS 1Add
BLAST
Domaini682 – 74261CBS 2Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi167 – 1715Selectivity filter part_1 By similarity
Motifi209 – 2135Selectivity filter part_2 By similarity
Motifi453 – 4575Selectivity filter part_3 By similarity

Sequence similaritiesi

Contains 2 CBS domains.

Keywords - Domaini

CBS domain, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0038.
GeneTreeiENSGT00620000087693.
HOGENOMiHOG000164493.
HOVERGENiHBG050984.
InParanoidiB1AXN0.
KOiK05012.
OrthoDBiEOG7MWGXT.
PhylomeDBiQ9WVD4.
TreeFamiTF313867.

Family and domain databases

Gene3Di1.10.3080.10. 3 hits.
InterProiIPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
IPR002247. Cl_channel-5.
[Graphical view]
PfamiPF00571. CBS. 2 hits.
PF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSiPR00762. CLCHANNEL.
PR01116. CLCHANNEL5.
SMARTiSM00116. CBS. 2 hits.
[Graphical view]
SUPFAMiSSF81340. SSF81340. 3 hits.
PROSITEiPS51371. CBS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WVD4-1 [UniParc]FASTAAdd to Basket

« Hide

MDFLEEPIPG VGTYDDFNTI DWVREKSRDR DRHREITNKS KESTWALIHS    50
VSDAFSGWLL MLLIGLLSGS LAGLIDISAH WMTDLKEGIC TGGFWFNHEH 100
CCWNSEHVTF EHRDKCPEWN SWAQLIINTD QGAFAYIVNY FMYVLWALLF 150
AFLAVSLVKA FAPYACGSGI PEIKTILSGF IIRGYLGKWT LVIKTITLVL 200
AVSSGLSLGK EGPLVHVACC CGNILCHCFN KYRKNEAKRR EVLSAAAAAG 250
VSVAFGAPIG GVLFSLEEVS YYFPLKTLWR SFFAALVAAF TLRSINPFGN 300
SRLVLFYVEF HTPWHLFELV PFIVLGIFGG LWGALFIRTN IAWCRKRKTT 350
QLGKYPVVEV LIVTAITAIL AFPNEYTRMS TSELISELFN DCGLLDSSKL 400
CDYENHFNTS KGGELPDRPA GVGIYSAMWQ LALTLILKIV ITIFTFGMKI 450
PSGLFIPSMA VGAIAGRLLG VGMEQLAYYH HDWGIFNSWC SQGADCITPG 500
LYAMVGAAAC LGGVTRMTVS LVVIMFELTG GLEYIVPLMA AAMTSKWVAD 550
ALGREGIYDA HIRLNGYPFL EAKEEFAHKT LAMDVMKPRR NDPLLTVLTQ 600
DSMTVEDVET IISETTYSGF PVVVSRESQR LVGFVLRRDL IISIENARKK 650
QDGVVSTSII YFTEHSPPMP PYTPPTLKLR NILDLSPFTV TDLTPMEIVV 700
DIFRKLGLRQ CLVTHNGRLL GIITKKDVLK HIAQMANQDP DSILFN 746
Length:746
Mass (Da):83,101
Last modified:November 1, 1999 - v1
Checksum:iD8F3AE4FFC331A08
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF134117 mRNA. Translation: AAD28473.1.
AL808124 Genomic DNA. Translation: CAM15287.1.
CCDSiCCDS29963.1.
RefSeqiNP_057900.3. NM_016691.4.
UniGeneiMm.486257.

Genome annotation databases

EnsembliENSMUST00000004428; ENSMUSP00000004428; ENSMUSG00000004317.
GeneIDi12728.
KEGGimmu:12728.
UCSCiuc009sle.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF134117 mRNA. Translation: AAD28473.1 .
AL808124 Genomic DNA. Translation: CAM15287.1 .
CCDSi CCDS29963.1.
RefSeqi NP_057900.3. NM_016691.4.
UniGenei Mm.486257.

3D structure databases

ProteinModelPortali Q9WVD4.
SMRi Q9WVD4. Positions 132-737.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9WVD4. 1 interaction.
MINTi MINT-4998506.
STRINGi 10090.ENSMUSP00000111412.

Proteomic databases

MaxQBi Q9WVD4.
PaxDbi Q9WVD4.
PRIDEi Q9WVD4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000004428 ; ENSMUSP00000004428 ; ENSMUSG00000004317 .
GeneIDi 12728.
KEGGi mmu:12728.
UCSCi uc009sle.3. mouse.

Organism-specific databases

CTDi 1184.
MGIi MGI:99486. Clcn5.

Phylogenomic databases

eggNOGi COG0038.
GeneTreei ENSGT00620000087693.
HOGENOMi HOG000164493.
HOVERGENi HBG050984.
InParanoidi B1AXN0.
KOi K05012.
OrthoDBi EOG7MWGXT.
PhylomeDBi Q9WVD4.
TreeFami TF313867.

Enzyme and pathway databases

Reactomei REACT_196640. Stimuli-sensing channels.

Miscellaneous databases

ChiTaRSi CLCN5. mouse.
NextBioi 282026.
PROi Q9WVD4.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9WVD4.
Bgeei Q9WVD4.
CleanExi MM_CLCN5.
Genevestigatori Q9WVD4.

Family and domain databases

Gene3Di 1.10.3080.10. 3 hits.
InterProi IPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
IPR002247. Cl_channel-5.
[Graphical view ]
Pfami PF00571. CBS. 2 hits.
PF00654. Voltage_CLC. 1 hit.
[Graphical view ]
PRINTSi PR00762. CLCHANNEL.
PR01116. CLCHANNEL5.
SMARTi SM00116. CBS. 2 hits.
[Graphical view ]
SUPFAMi SSF81340. SSF81340. 3 hits.
PROSITEi PS51371. CBS. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of novel promoter and enhancer elements of the mouse homologue of the Dent disease gene, CLCN5, implicated in X-linked hereditary nephrolithiasis."
    Tanaka K., Fisher S.E., Craig I.W.
    Genomics 58:281-292(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.

Entry informationi

Entry nameiCLCN5_MOUSE
AccessioniPrimary (citable) accession number: Q9WVD4
Secondary accession number(s): B1AXN0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1999
Last modified: September 3, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons. The absence of conserved gating glutamate residues is typical for family members that function as channels By similarity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi