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Protein

Chloride anion exchanger

Gene

Slc26a3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Chloride/bicarbonate exchanger. Mediates the efficient absorption of chloride ions in the colon, participating in fluid homeostasis. Plays a role in the chloride and bicarbonate homeostasis during sperm epididymal maturation and capacitation.2 Publications

GO - Molecular functioni

  1. anion:anion antiporter activity Source: MGI
  2. bicarbonate transmembrane transporter activity Source: UniProtKB
  3. chloride transmembrane transporter activity Source: UniProtKB
  4. secondary active sulfate transmembrane transporter activity Source: InterPro

GO - Biological processi

  1. anion transmembrane transport Source: GOC
  2. cellular response to cAMP Source: UniProtKB
  3. chloride transmembrane transport Source: GOC
  4. intracellular pH elevation Source: UniProtKB
  5. membrane hyperpolarization Source: UniProtKB
  6. sperm capacitation Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Antiport, Transport

Keywords - Ligandi

Chloride

Enzyme and pathway databases

ReactomeiREACT_350869. Multifunctional anion exchangers.

Protein family/group databases

TCDBi2.A.53.2.3. the sulfate permease (sulp) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Chloride anion exchanger
Alternative name(s):
Down-regulated in adenoma
Short name:
Protein DRA
Solute carrier family 26 member 3
Gene namesi
Name:Slc26a3
Synonyms:Dra
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:107181. Slc26a3.

Subcellular locationi

Apical cell membrane By similarity; Multi-pass membrane protein By similarity. Membrane 1 Publication; Multi-pass membrane protein 1 Publication
Note: Localized in sperm membranes. Midpiece of sperm tail. Colocalizes with CFTR at the midpiece of sperm tail.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei77 – 9721HelicalSequence AnalysisAdd
BLAST
Transmembranei100 – 12021HelicalSequence AnalysisAdd
BLAST
Transmembranei125 – 14521HelicalSequence AnalysisAdd
BLAST
Topological domaini146 – 17530ExtracellularSequence AnalysisAdd
BLAST
Transmembranei176 – 19621HelicalSequence AnalysisAdd
BLAST
Transmembranei198 – 21821HelicalSequence AnalysisAdd
BLAST
Transmembranei259 – 27921HelicalSequence AnalysisAdd
BLAST
Transmembranei286 – 30621HelicalSequence AnalysisAdd
BLAST
Transmembranei343 – 36321HelicalSequence AnalysisAdd
BLAST
Transmembranei375 – 39521HelicalSequence AnalysisAdd
BLAST
Transmembranei412 – 43221HelicalSequence AnalysisAdd
BLAST
Transmembranei439 – 45921HelicalSequence AnalysisAdd
BLAST
Transmembranei470 – 49021HelicalSequence AnalysisAdd
BLAST
Transmembranei644 – 66421HelicalSequence AnalysisAdd
BLAST
Transmembranei702 – 72221HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. apical plasma membrane Source: UniProtKB-SubCell
  2. brush border membrane Source: Ensembl
  3. integral component of plasma membrane Source: MGI
  4. membrane Source: UniProtKB
  5. sperm midpiece Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 757757Chloride anion exchangerPRO_0000080162Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi149 – 1491N-linked (GlcNAc...)Sequence Analysis
Glycosylationi161 – 1611N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

N-glycosylation is required for efficient cell surface expression, and protection from proteolytic degradation.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ9WVC8.
PRIDEiQ9WVC8.

Expressioni

Tissue specificityi

Expressed in spermatogenic cells.1 Publication

Gene expression databases

BgeeiQ9WVC8.
CleanExiMM_SLC26A3.
ExpressionAtlasiQ9WVC8. baseline and differential.
GenevestigatoriQ9WVC8.

Interactioni

Subunit structurei

Interacts with PDZK1 (By similarity). Interacts with CFTR, SLC26A6 and SLC9A3R1.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CftrP263613EBI-6895537,EBI-6115317
Slc26a6Q8CIW62EBI-6895537,EBI-6895517
Slc9a3r1P704412EBI-6895537,EBI-1184085

Protein-protein interaction databases

IntActiQ9WVC8. 3 interactions.
STRINGi10090.ENSMUSP00000001254.

Structurei

3D structure databases

ProteinModelPortaliQ9WVC8.
SMRiQ9WVC8. Positions 638-711.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini518 – 713196STASPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 STAS domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0659.
GeneTreeiENSGT00760000119026.
HOGENOMiHOG000006546.
HOVERGENiHBG000639.
InParanoidiQ9WVC8.
KOiK14078.
OMAiYIVARPV.
OrthoDBiEOG76T9QT.
PhylomeDBiQ9WVC8.
TreeFamiTF313784.

Family and domain databases

Gene3Di3.30.750.24. 2 hits.
InterProiIPR018045. S04_transporter_CS.
IPR030321. SLC26A3.
IPR002645. STAS_dom.
IPR001902. Sul_anion_transpt.
IPR030402. Sulfate_tra_GLY.
IPR011547. Sulph_transpt.
[Graphical view]
PANTHERiPTHR11814. PTHR11814. 1 hit.
PTHR11814:SF19. PTHR11814:SF19. 1 hit.
PfamiPF01740. STAS. 1 hit.
PF13792. Sulfate_tra_GLY. 1 hit.
PF00916. Sulfate_transp. 1 hit.
[Graphical view]
SUPFAMiSSF52091. SSF52091. 2 hits.
TIGRFAMsiTIGR00815. sulP. 1 hit.
PROSITEiPS01130. SLC26A. 1 hit.
PS50801. STAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WVC8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEAIGNQYV VARPVYSTKT FGEEFKKTHR HHKTFLDHLK GCCSCSSQKA
60 70 80 90 100
KKIALSLFPI ASWLPAYKIK EWLLSDIVSG ISTGLVAVLQ GLAFALLVNI
110 120 130 140 150
PPAYGLYAAF FPVITYFFLG TSRHISVGPF PVLSMMVGVV VTRVVSDPNA
160 170 180 190 200
SSELSSSSTE NDSFIEEKVM VAASVTVLSG IIQLLLGVLQ VGFVVIYLSE
210 220 230 240 250
SLISGFTTAA AIHVLVSQLK FMLQLPVPAY SDPFSIFKVL ESVFTQIQKT
260 270 280 290 300
NIADLVTSVI ILVVVFVFKE INQRYRSKLP VPIPIELIMT VIATGVSYGC
310 320 330 340 350
NFEDRFGVAV VGNMSLGFQP PITPSVEVFQ DTIGDSFGIA IVGFAVAFSV
360 370 380 390 400
ASVYSLKYDY PIDGNQELIA LGVSNIFTGA FKGFAGSTAL SRSGVQESTG
410 420 430 440 450
GKTQVAGLLS AVIVLIVIVA IGFLLQPLQK SVLAALALGN LKGMLMQFAE
460 470 480 490 500
IGRLWKKDKY DCLIWIMTFI FAIVLGLGLG LAASVAFQLL TIVFRTQFPK
510 520 530 540 550
CSTLANVGRS NIYKNKKNYA EVYEPEGVKI FRCPSPIYFA NIGFFKQKLI
560 570 580 590 600
DAVGFSPLRI LRKRNKALKK IRKLQKRGLI QMTPKGFICT SDGFKDSDEE
610 620 630 640 650
LDNNQIEELD QPINTTDLPF DIDWNGDLPL NITIPKISLH SLILDFSAVS
660 670 680 690 700
FLDVSSMRGL RTILQEFIRI KVDVYIVGTD DDFIDKLARC EFFDDEVTDS
710 720 730 740 750
IFFLTIHDAI LHILMKKDYS TSKFNSSQEK ERKFDFTINT NGGLRNRECQ

VPVETKF
Length:757
Mass (Da):83,590
Last modified:October 31, 1999 - v1
Checksum:i32B1AC648BE74A07
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF136751 mRNA. Translation: AAD42784.1.
BC139271 mRNA. Translation: AAI39272.1.
BC139273 mRNA. Translation: AAI39274.1.
CCDSiCCDS25863.1.
RefSeqiNP_067328.1. NM_021353.3.
UniGeneiMm.490454.

Genome annotation databases

EnsembliENSMUST00000001254; ENSMUSP00000001254; ENSMUSG00000001225.
GeneIDi13487.
KEGGimmu:13487.
UCSCiuc007nhj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF136751 mRNA. Translation: AAD42784.1.
BC139271 mRNA. Translation: AAI39272.1.
BC139273 mRNA. Translation: AAI39274.1.
CCDSiCCDS25863.1.
RefSeqiNP_067328.1. NM_021353.3.
UniGeneiMm.490454.

3D structure databases

ProteinModelPortaliQ9WVC8.
SMRiQ9WVC8. Positions 638-711.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9WVC8. 3 interactions.
STRINGi10090.ENSMUSP00000001254.

Protein family/group databases

TCDBi2.A.53.2.3. the sulfate permease (sulp) family.

Proteomic databases

MaxQBiQ9WVC8.
PRIDEiQ9WVC8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000001254; ENSMUSP00000001254; ENSMUSG00000001225.
GeneIDi13487.
KEGGimmu:13487.
UCSCiuc007nhj.1. mouse.

Organism-specific databases

CTDi1811.
MGIiMGI:107181. Slc26a3.

Phylogenomic databases

eggNOGiCOG0659.
GeneTreeiENSGT00760000119026.
HOGENOMiHOG000006546.
HOVERGENiHBG000639.
InParanoidiQ9WVC8.
KOiK14078.
OMAiYIVARPV.
OrthoDBiEOG76T9QT.
PhylomeDBiQ9WVC8.
TreeFamiTF313784.

Enzyme and pathway databases

ReactomeiREACT_350869. Multifunctional anion exchangers.

Miscellaneous databases

NextBioi283993.
PROiQ9WVC8.
SOURCEiSearch...

Gene expression databases

BgeeiQ9WVC8.
CleanExiMM_SLC26A3.
ExpressionAtlasiQ9WVC8. baseline and differential.
GenevestigatoriQ9WVC8.

Family and domain databases

Gene3Di3.30.750.24. 2 hits.
InterProiIPR018045. S04_transporter_CS.
IPR030321. SLC26A3.
IPR002645. STAS_dom.
IPR001902. Sul_anion_transpt.
IPR030402. Sulfate_tra_GLY.
IPR011547. Sulph_transpt.
[Graphical view]
PANTHERiPTHR11814. PTHR11814. 1 hit.
PTHR11814:SF19. PTHR11814:SF19. 1 hit.
PfamiPF01740. STAS. 1 hit.
PF13792. Sulfate_tra_GLY. 1 hit.
PF00916. Sulfate_transp. 1 hit.
[Graphical view]
SUPFAMiSSF52091. SSF52091. 2 hits.
TIGRFAMsiTIGR00815. sulP. 1 hit.
PROSITEiPS01130. SLC26A. 1 hit.
PS50801. STAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse down-regulated in adenoma (DRA) is an intestinal Cl(-)/HCO(3)(-) exchanger and is up-regulated in colon of mice lacking the NHE3 Na(+)/H(+) exchanger."
    Melvin J.E., Park K., Richardson L., Schultheis P.J., Shull G.E.
    J. Biol. Chem. 274:22855-22861(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    Strain: FVB/N.
    Tissue: Colon.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. "Participation of the Cl-/HCO(3)- exchangers SLC26A3 and SLC26A6, the Cl- channel CFTR, and the regulatory factor SLC9A3R1 in mouse sperm capacitation."
    Chavez J.C., Hernandez-Gonzalez E.O., Wertheimer E., Visconti P.E., Darszon A., Trevino C.L.
    Biol. Reprod. 86:1-14(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CFTR; SLC26A6 AND SLC9A3R1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiS26A3_MOUSE
AccessioniPrimary (citable) accession number: Q9WVC8
Secondary accession number(s): B2RTD9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 4, 2002
Last sequence update: October 31, 1999
Last modified: March 31, 2015
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.