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Q9WVC8 (S26A3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chloride anion exchanger
Alternative name(s):
Down-regulated in adenoma
Short name=Protein DRA
Solute carrier family 26 member 3
Gene names
Name:Slc26a3
Synonyms:Dra
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length757 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Chloride/bicarbonate exchanger. Mediates the efficient absorption of chloride ions in the colon, participating in fluid homeostasis. Plays a role in the chloride and bicarbonate homeostasis during sperm epididymal maturation and capacitation. Ref.1 Ref.3

Subunit structure

Interacts with PDZK1 By similarity. Interacts with CFTR, SLC26A6 and SLC9A3R1. Ref.3

Subcellular location

Apical cell membrane; Multi-pass membrane protein By similarity. Membrane; Multi-pass membrane protein. Note: Localized in sperm membranes. Midpiece of sperm tail. Colocalizes with CFTR at the midpiece of sperm tail. Ref.3

Tissue specificity

Expressed in spermatogenic cells. Ref.3

Post-translational modification

N-glycosylation is required for efficient cell surface expression, and protection from proteolytic degradation By similarity.

Sequence similarities

Belongs to the SLC26A/SulP transporter (TC 2.A.53) family. [View classification]

Contains 1 STAS domain.

Ontologies

Keywords
   Biological processAntiport
Transport
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   LigandChloride
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processbicarbonate transport

Inferred from mutant phenotype Ref.3. Source: GOC

cellular response to cAMP

Inferred from direct assay Ref.3. Source: UniProtKB

chloride transmembrane transport

Inferred from mutant phenotype Ref.3. Source: GOC

chloride transport

Inferred from mutant phenotype Ref.3. Source: GOC

intracellular pH elevation

Inferred from mutant phenotype Ref.3. Source: UniProtKB

membrane hyperpolarization

Inferred from mutant phenotype Ref.3. Source: UniProtKB

sperm capacitation

Inferred from mutant phenotype Ref.3. Source: UniProtKB

   Cellular_componentapical plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

brush border membrane

Inferred from electronic annotation. Source: Ensembl

integral component of plasma membrane

Inferred from sequence or structural similarity Ref.1. Source: MGI

membrane

Inferred from direct assay Ref.3. Source: UniProtKB

sperm midpiece

Inferred from direct assay Ref.3. Source: UniProtKB

   Molecular_functionanion:anion antiporter activity

Inferred from direct assay Ref.1. Source: MGI

bicarbonate transmembrane transporter activity

Inferred from mutant phenotype Ref.3. Source: UniProtKB

chloride transmembrane transporter activity

Inferred from mutant phenotype Ref.3. Source: UniProtKB

secondary active sulfate transmembrane transporter activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 757757Chloride anion exchanger
PRO_0000080162

Regions

Transmembrane77 – 9721Helical; Potential
Transmembrane100 – 12021Helical; Potential
Transmembrane125 – 14521Helical; Potential
Topological domain146 – 17530Extracellular Potential
Transmembrane176 – 19621Helical; Potential
Transmembrane198 – 21821Helical; Potential
Transmembrane259 – 27921Helical; Potential
Transmembrane286 – 30621Helical; Potential
Transmembrane343 – 36321Helical; Potential
Transmembrane375 – 39521Helical; Potential
Transmembrane412 – 43221Helical; Potential
Transmembrane439 – 45921Helical; Potential
Transmembrane470 – 49021Helical; Potential
Transmembrane644 – 66421Helical; Potential
Transmembrane702 – 72221Helical; Potential
Domain518 – 713196STAS

Amino acid modifications

Glycosylation1491N-linked (GlcNAc...) Potential
Glycosylation1611N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q9WVC8 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 32B1AC648BE74A07

FASTA75783,590
        10         20         30         40         50         60 
MIEAIGNQYV VARPVYSTKT FGEEFKKTHR HHKTFLDHLK GCCSCSSQKA KKIALSLFPI 

        70         80         90        100        110        120 
ASWLPAYKIK EWLLSDIVSG ISTGLVAVLQ GLAFALLVNI PPAYGLYAAF FPVITYFFLG 

       130        140        150        160        170        180 
TSRHISVGPF PVLSMMVGVV VTRVVSDPNA SSELSSSSTE NDSFIEEKVM VAASVTVLSG 

       190        200        210        220        230        240 
IIQLLLGVLQ VGFVVIYLSE SLISGFTTAA AIHVLVSQLK FMLQLPVPAY SDPFSIFKVL 

       250        260        270        280        290        300 
ESVFTQIQKT NIADLVTSVI ILVVVFVFKE INQRYRSKLP VPIPIELIMT VIATGVSYGC 

       310        320        330        340        350        360 
NFEDRFGVAV VGNMSLGFQP PITPSVEVFQ DTIGDSFGIA IVGFAVAFSV ASVYSLKYDY 

       370        380        390        400        410        420 
PIDGNQELIA LGVSNIFTGA FKGFAGSTAL SRSGVQESTG GKTQVAGLLS AVIVLIVIVA 

       430        440        450        460        470        480 
IGFLLQPLQK SVLAALALGN LKGMLMQFAE IGRLWKKDKY DCLIWIMTFI FAIVLGLGLG 

       490        500        510        520        530        540 
LAASVAFQLL TIVFRTQFPK CSTLANVGRS NIYKNKKNYA EVYEPEGVKI FRCPSPIYFA 

       550        560        570        580        590        600 
NIGFFKQKLI DAVGFSPLRI LRKRNKALKK IRKLQKRGLI QMTPKGFICT SDGFKDSDEE 

       610        620        630        640        650        660 
LDNNQIEELD QPINTTDLPF DIDWNGDLPL NITIPKISLH SLILDFSAVS FLDVSSMRGL 

       670        680        690        700        710        720 
RTILQEFIRI KVDVYIVGTD DDFIDKLARC EFFDDEVTDS IFFLTIHDAI LHILMKKDYS 

       730        740        750 
TSKFNSSQEK ERKFDFTINT NGGLRNRECQ VPVETKF 

« Hide

References

« Hide 'large scale' references
[1]"Mouse down-regulated in adenoma (DRA) is an intestinal Cl(-)/HCO(3)(-) exchanger and is up-regulated in colon of mice lacking the NHE3 Na(+)/H(+) exchanger."
Melvin J.E., Park K., Richardson L., Schultheis P.J., Shull G.E.
J. Biol. Chem. 274:22855-22861(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
Strain: FVB/N.
Tissue: Colon.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[3]"Participation of the Cl-/HCO(3)- exchangers SLC26A3 and SLC26A6, the Cl- channel CFTR, and the regulatory factor SLC9A3R1 in mouse sperm capacitation."
Chavez J.C., Hernandez-Gonzalez E.O., Wertheimer E., Visconti P.E., Darszon A., Trevino C.L.
Biol. Reprod. 86:1-14(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH CFTR; SLC26A6 AND SLC9A3R1, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF136751 mRNA. Translation: AAD42784.1.
BC139271 mRNA. Translation: AAI39272.1.
BC139273 mRNA. Translation: AAI39274.1.
RefSeqNP_067328.1. NM_021353.3.
UniGeneMm.490454.

3D structure databases

ProteinModelPortalQ9WVC8.
SMRQ9WVC8. Positions 638-711.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9WVC8. 3 interactions.
STRING10090.ENSMUSP00000001254.

Protein family/group databases

TCDB2.A.53.2.3. the sulfate permease (sulp) family.

Proteomic databases

PRIDEQ9WVC8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000001254; ENSMUSP00000001254; ENSMUSG00000001225.
GeneID13487.
KEGGmmu:13487.
UCSCuc007nhj.1. mouse.

Organism-specific databases

CTD1811.
MGIMGI:107181. Slc26a3.

Phylogenomic databases

eggNOGCOG0659.
GeneTreeENSGT00750000117367.
HOGENOMHOG000006546.
HOVERGENHBG000639.
InParanoidB2RTD9.
KOK14078.
OMAYIVARPV.
OrthoDBEOG76T9QT.
PhylomeDBQ9WVC8.
TreeFamTF313784.

Gene expression databases

BgeeQ9WVC8.
CleanExMM_SLC26A3.
GenevestigatorQ9WVC8.

Family and domain databases

Gene3D3.30.750.24. 2 hits.
InterProIPR018045. S04_transporter_CS.
IPR002645. STAS_dom.
IPR001902. SulP_transpt.
IPR011547. Sulph_transpt.
[Graphical view]
PfamPF01740. STAS. 1 hit.
PF00916. Sulfate_transp. 1 hit.
[Graphical view]
SUPFAMSSF52091. SSF52091. 2 hits.
TIGRFAMsTIGR00815. sulP. 1 hit.
PROSITEPS01130. SLC26A. 1 hit.
PS50801. STAS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio283993.
PROQ9WVC8.
SOURCESearch...

Entry information

Entry nameS26A3_MOUSE
AccessionPrimary (citable) accession number: Q9WVC8
Secondary accession number(s): B2RTD9
Entry history
Integrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: November 1, 1999
Last modified: April 16, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot