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Protein

A-kinase anchor protein 6

Gene

Akap6

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to type II regulatory subunits of protein kinase A and anchors/targets them to the nuclear membrane or sarcoplasmic reticulum. May act as an adapter for assembling multiprotein complexes (By similarity).By similarity

GO - Molecular functioni

  • adenylate cyclase binding Source: BHF-UCL
  • enzyme binding Source: BHF-UCL
  • ion channel binding Source: BHF-UCL
  • protein anchor Source: BHF-UCL
  • protein complex scaffold Source: BHF-UCL
  • protein kinase A binding Source: BHF-UCL
  • protein kinase A regulatory subunit binding Source: BHF-UCL
  • protein N-terminus binding Source: RGD
  • protein phosphatase 2A binding Source: BHF-UCL

GO - Biological processi

  • cellular protein complex localization Source: BHF-UCL
  • cellular response to cytokine stimulus Source: BHF-UCL
  • cellular response to epinephrine stimulus Source: BHF-UCL
  • negative regulation of cAMP biosynthetic process Source: RGD
  • positive regulation of calcineurin-NFAT signaling cascade Source: BHF-UCL
  • positive regulation of cAMP-dependent protein kinase activity Source: BHF-UCL
  • positive regulation of cell growth Source: BHF-UCL
  • positive regulation of cell growth involved in cardiac muscle cell development Source: RGD
  • positive regulation of NFAT protein import into nucleus Source: BHF-UCL
  • positive regulation of protein phosphatase type 2B activity Source: RGD
  • positive regulation of protein phosphorylation Source: BHF-UCL
  • positive regulation of release of sequestered calcium ion into cytosol Source: BHF-UCL
  • positive regulation of ryanodine-sensitive calcium-release channel activity Source: BHF-UCL
  • regulation of cardiac muscle cell contraction Source: BHF-UCL
  • regulation of protein kinase A signaling Source: RGD
  • regulation of relaxation of cardiac muscle Source: BHF-UCL
  • regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum Source: BHF-UCL
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
A-kinase anchor protein 6
Short name:
AKAP-6
Alternative name(s):
Protein kinase A-anchoring protein 6
Short name:
PRKA6
mAKAP
Gene namesi
Name:Akap6
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi69412. Akap6.

Subcellular locationi

  • Sarcoplasmic reticulum
  • Nucleus membrane

  • Note: In heart muscle. Participation of multiple targeting signals allow correct intracellular targeting. These may be repeated motifs rich in basic and hydrophobic amino acids, palmitoylated/myristoylated motifs or alternatively splice targeting sequences.

GO - Cellular componenti

  • caveola Source: RGD
  • cytoplasm Source: BHF-UCL
  • intercalated disc Source: BHF-UCL
  • junctional sarcoplasmic reticulum membrane Source: BHF-UCL
  • nuclear envelope Source: BHF-UCL
  • nuclear membrane Source: RGD
  • nuclear outer membrane Source: RGD
  • nucleus Source: BHF-UCL
  • perinuclear region of cytoplasm Source: BHF-UCL
  • protein complex Source: RGD
  • sarcolemma Source: BHF-UCL
  • sarcoplasmic reticulum Source: MGI
  • T-tubule Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nucleus, Sarcoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 23142314A-kinase anchor protein 6PRO_0000064531Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1072 – 10721PhosphoserineCombined sources
Modified residuei1568 – 15681PhosphoserineCombined sources
Modified residuei1593 – 15931PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9WVC7.
PRIDEiQ9WVC7.

PTM databases

iPTMnetiQ9WVC7.
PhosphoSiteiQ9WVC7.

Interactioni

Subunit structurei

Interacts with RII subunit of PKA, phosphatase 2B (calcineurin) and AKAP79.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Ppp3cbP206512EBI-7559840,EBI-7400670
Ppp3cbP484532EBI-7559840,EBI-642618From a different organism.

GO - Molecular functioni

  • adenylate cyclase binding Source: BHF-UCL
  • enzyme binding Source: BHF-UCL
  • ion channel binding Source: BHF-UCL
  • protein anchor Source: BHF-UCL
  • protein complex scaffold Source: BHF-UCL
  • protein kinase A binding Source: BHF-UCL
  • protein kinase A regulatory subunit binding Source: BHF-UCL
  • protein N-terminus binding Source: RGD
  • protein phosphatase 2A binding Source: BHF-UCL

Protein-protein interaction databases

IntActiQ9WVC7. 2 interactions.
MINTiMINT-4998500.
STRINGi10116.ENSRNOP00000006562.

Structurei

3D structure databases

ProteinModelPortaliQ9WVC7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati768 – 84780Spectrin 1Add
BLAST
Repeati1033 – 1148116Spectrin 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2062 – 207514PKA-RII subunit binding domainAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi516 – 60691Ser-richAdd
BLAST
Compositional biasi1558 – 1699142Ser-richAdd
BLAST

Domaini

RII-alpha binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer.

Sequence similaritiesi

Contains 2 spectrin repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IJDE. Eukaryota.
ENOG410XT58. LUCA.
HOGENOMiHOG000001568.
HOVERGENiHBG050480.
InParanoidiQ9WVC7.
KOiK16523.

Family and domain databases

InterProiIPR026696. AKAP6/CEP68.
IPR018159. Spectrin/alpha-actinin.
IPR002017. Spectrin_repeat.
[Graphical view]
PANTHERiPTHR14514. PTHR14514. 1 hit.
PfamiPF00435. Spectrin. 1 hit.
[Graphical view]
SMARTiSM00150. SPEC. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WVC7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLTMSVTLSP LRSQGPDPMA TDASPMAINM TPTVEQEEGE GEEAVKAIDA
60 70 80 90 100
EQQYGKPPPL HTAADWKIVL HLPEIETWLR MTSERVRDLT YSVQQDADSK
110 120 130 140 150
HVDVHLVQLK DICEDISDHV EQIHALLETE FSLKLLSYSV NVIVDIHAVQ
160 170 180 190 200
LLWHQLRVSV LVLRERILQG LQDANGNYTR QTDILQAFSE ETTEGRLDSL
210 220 230 240 250
TEVDDSGQLT IKCSQDYLSL DCGITAFELS DYSPSEDLLG GLGDMTTSQA
260 270 280 290 300
KTKSFDSWSY SEMEKEFPEL IRSVGLLTVA TEPVPSSCGE ANEDSSQASL
310 320 330 340 350
SDDHKGEHGE DGAPVPGQQL DSTVGMSSLD GTLANAAEHP SETAKQDSTS
360 370 380 390 400
SPQLGAKKTQ PGPCEITTPK RSIRDCFNYN EDSPTQPTLP KRGLFLKETQ
410 420 430 440 450
KNERKGSDRK GQVVDLKPEL SRSTPSLVDP PDRSKLCLVL QSSYPSSPSA
460 470 480 490 500
ASQSYECLHK VGLGNLENIV RSHIKEISSS LGRLTDCHKE KLRLKKPHKT
510 520 530 540 550
LAEVSLCRIP KQGGGSGKRS ESTGSSAGPS MVSPGAPKAT MRPETDSAST
560 570 580 590 600
ASGGLCHQRN RSGQLPVQSK ASSSPPCSHS SESSLGSDSI KSPVPLLSKN
610 620 630 640 650
KSQKSSPPAP CHATQNGQVV EAWYGSDEYL ALPSHLKQTE VLALKLESLT
660 670 680 690 700
KLLPQKPRGE TIQDIDDWEL SEMNSDSEIY PTYHIKKKHT RLGTVSPSSS
710 720 730 740 750
SDIASSLGES IESGPLSDIL SDEDLCLPLS SVKKFTDEKS ERPSSSEKNE
760 770 780 790 800
SHSATRSALI QKLMHDIQHQ ENYEAIWERI EGFVNKLDEF IQWLNEAMET
810 820 830 840 850
TENWTPPKAE TDSLRLYLET HLSFKLNVDS HCALKEAVEE EGHQLLELVV
860 870 880 890 900
SHKAGLKDTL RMIASQWKEL QRQIKRQHSW ILRALDTIKA EILATDVSVE
910 920 930 940 950
DEEGTGSPKA EVQLCHLETQ RDAVEQMSLK LYSEQYTSGS KRKEEFANMS
960 970 980 990 1000
KAHAEGSNGL LDFDSEYQEL WDWLIDMESL VMDSHDLMMS EEQQQHLYKR
1010 1020 1030 1040 1050
YSVEMSIRHL KKSELLSKVE ALKKGGLSLP DDILEKVDSI NEKWELLGKT
1060 1070 1080 1090 1100
LREKIQDTIA GHSGSGPRDL LSPESGSLVR QLEVRIKELK RWLRDTELFI
1110 1120 1130 1140 1150
FNSCLRQEKE GTSAEKQLQY FKSLCREIKQ RRRGVASILR LCQHLLDDRD
1160 1170 1180 1190 1200
TCNLNADHQP MQLIIVNLER RWEAIVMQAV QWQTRLQKKM GKESETLNVI
1210 1220 1230 1240 1250
DPGLMDLNGM SEDALEWDET DISNKLISVH EESNDLDQDP EPMLPAVKLE
1260 1270 1280 1290 1300
ETHHKDSGYE EEAGDCGGSP YTSNITAPSS PHIYQVYSLH NVELHEDSHT
1310 1320 1330 1340 1350
PFLKSSPKFT GTTQPTVLTK SLSKDSSFSS TKSLPDLLGG SGLVRPYSCH
1360 1370 1380 1390 1400
SGDLSQNSGS ESGIVSEGDN EMPTNSDMSL FSMVDGSPSN PETEHPDPQM
1410 1420 1430 1440 1450
GDAANVLEQK FKDNGESIKL SSVSRASVSP VGCVNGKAGD LNSVTKHTAD
1460 1470 1480 1490 1500
CLGEELQGKH DVFTFYDYSY LQGSKLKLPM IMKQPQSEKA HVEDPLLGGF
1510 1520 1530 1540 1550
YFDKKSCKAK HQASESQPDA PPHERILASA PHEMGRSAYK SSDIEKTFTG
1560 1570 1580 1590 1600
IQSARQLSLL SRSSSVESLS PGGDLFGLGI FKNGSDSLQR STSLESWLTS
1610 1620 1630 1640 1650
YKSNEDLFSC HSSGDISVSS GSVGELSKRT LDLLNRLENI QSPSEQKIKR
1660 1670 1680 1690 1700
SVSDMTLQSS SQKMPFAGQM SLDVASSINE DSPASLTELS SSDELSLCSE
1710 1720 1730 1740 1750
DIVLHKNKIP ESNASFRKRL NRSVADESDV NVSMIVNVSC TSACTDDEDD
1760 1770 1780 1790 1800
SDLLSSSTLT LTEEELCLKD EDDDSSIATD DEIYEESNLM SGLDYIKNEL
1810 1820 1830 1840 1850
QTWIRPKLSL TREKKRSGVT DEIKVNKDGG GNEKANPSDT LDIEALLNGS
1860 1870 1880 1890 1900
IRCLSENNGN GKTPPRTHGS GTKGENKKST YDVSKDPHVA DMENGNIEST
1910 1920 1930 1940 1950
PEREREKPQG LPEVSENLAS NVKTISESEL SEYEAVMDGS EDSSVARKEF
1960 1970 1980 1990 2000
CPPNDRHPPQ MGPKLQHPEN QSGDCKPVQN PCPGLLSEAG VGSRQDSNGL
2010 2020 2030 2040 2050
KSLPNDAPSG ARKPAGCCLL EQNETEESAS ISSNASCCNC KPDVFHQKDD
2060 2070 2080 2090 2100
EDCSVHDFVK EIIDMASTAL KSKSQPESEV AAPTSLTQIK EKVLEHSHRP
2110 2120 2130 2140 2150
IHLRKGDFYS YLSLSSHDSD CGEVTNYIDE KSSTPLPPDA VDSGLDDKED
2160 2170 2180 2190 2200
MDCFFEACVE DEPVNEEAGL PGALPNESAI EDGAEQKSEQ KTASSPVLSD
2210 2220 2230 2240 2250
KTDLVPLSGL SPQKGADDAK EGDDVSHTSQ GCAESTEPTT PSGKANAEGR
2260 2270 2280 2290 2300
SRMQGVSATP EENAASAKPK IQAFSLNAKQ PKGKVAMRYP SPQTLTCKEK
2310
LVNFHEDRHS NMHR
Length:2,314
Mass (Da):254,352
Last modified:November 1, 1999 - v1
Checksum:i2FB01DE0E2DDFA37
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF139518 mRNA. Translation: AAD39150.1.
RefSeqiNP_072140.1. NM_022618.1.
UniGeneiRn.147642.

Genome annotation databases

GeneIDi64553.
KEGGirno:64553.
UCSCiRGD:69412. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF139518 mRNA. Translation: AAD39150.1.
RefSeqiNP_072140.1. NM_022618.1.
UniGeneiRn.147642.

3D structure databases

ProteinModelPortaliQ9WVC7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9WVC7. 2 interactions.
MINTiMINT-4998500.
STRINGi10116.ENSRNOP00000006562.

PTM databases

iPTMnetiQ9WVC7.
PhosphoSiteiQ9WVC7.

Proteomic databases

PaxDbiQ9WVC7.
PRIDEiQ9WVC7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi64553.
KEGGirno:64553.
UCSCiRGD:69412. rat.

Organism-specific databases

CTDi9472.
RGDi69412. Akap6.

Phylogenomic databases

eggNOGiENOG410IJDE. Eukaryota.
ENOG410XT58. LUCA.
HOGENOMiHOG000001568.
HOVERGENiHBG050480.
InParanoidiQ9WVC7.
KOiK16523.

Miscellaneous databases

PROiQ9WVC7.

Family and domain databases

InterProiIPR026696. AKAP6/CEP68.
IPR018159. Spectrin/alpha-actinin.
IPR002017. Spectrin_repeat.
[Graphical view]
PANTHERiPTHR14514. PTHR14514. 1 hit.
PfamiPF00435. Spectrin. 1 hit.
[Graphical view]
SMARTiSM00150. SPEC. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAKAP6_RAT
AccessioniPrimary (citable) accession number: Q9WVC7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: November 1, 1999
Last modified: June 8, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.