Q9WVB9 (DVL1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Segment polarity protein dishevelled homolog DVL-1
Short name=Dishevelled-1

Alternative name(s):
DSH homolog 1

Gene names

Name:

Dvl1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	695 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes. Required for LEF1 activation upon WNT1 and WNT3A signaling. DVL1 and PAK1 form a ternary complex with MUSK which is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ) By similarity.
Subunit structure	Interacts with BRD7 and INVS. Interacts through its PDZ domain with the C-terminal regions of VANGL1, VANGL2 and CCDC88C/DAPLE. Interacts (via PDZ domain) with NXN. Interacts with CXXC4. Interacts with ARRB1; the interaction is enhanced by phosphorylation of DVL1. Interacts with CYLD. Interacts (via PDZ domain) with RYK. Self-associates (via DIX domain) and forms higher homooligomers. Interacts (via PDZ domain) with DACT1 and FZD7, where DACT1 and FZD7 compete for the same binding site. Interacts (via DEP domain) with MUSK; the interaction is direct and mediates the formation a DVL1, MUSK and PAK1 ternary complex involved in AChR clustering By similarity. Ref.2
Subcellular location	Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cytoplasm › cytosol By similarity. Cytoplasmic vesicle By similarity. Note: Localizes at the cell membrane upon interaction with frizzled family members By similarity.
Domain	The DIX domain promotes homooligomerization By similarity. The DEP domain mediates interaction with the cell membrane By similarity.
Post-translational modification	Ubiquitinated; undergoes both 'Lys-48'-linked ubiquitination, leading to its subsequent degradation by the ubiquitin-proteasome pathway, and 'Lys-63'-linked ubiquitination. The interaction with INVS is required for ubiquitination. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains By similarity.
Sequence similarities	Belongs to the DSH family. Contains 1 DEP domain. Contains 1 DIX domain. Contains 1 PDZ (DHR) domain.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Cell membrane Cytoplasm Cytoplasmic vesicle Membrane
Molecular function	Developmental protein
PTM	Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	axon extension Inferred from Biological aspect of Ancestor. Source: RefGenome axon guidance Inferred from Biological aspect of Ancestor. Source: RefGenome canonical Wnt receptor signaling pathway Inferred from Biological aspect of Ancestor. Source: RefGenome cochlea morphogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome collateral sprouting Inferred from Biological aspect of Ancestor. Source: RefGenome dendrite morphogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome intracellular signal transduction Inferred from electronic annotation. Source: InterPro planar cell polarity pathway involved in neural tube closure Inferred from Biological aspect of Ancestor. Source: RefGenome positive regulation of transcription, DNA-dependent Inferred from Biological aspect of Ancestor. Source: RefGenome skeletal muscle acetylcholine-gated channel clustering Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular component	axon Inferred from direct assay. Source: RGD cell cortex Inferred from Biological aspect of Ancestor. Source: RefGenome clathrin-coated vesicle Inferred from Biological aspect of Ancestor. Source: RefGenome cytosol Inferred from electronic annotation. Source: UniProtKB-SubCell dendrite Inferred from direct assay. Source: RGD growth cone Inferred from direct assay. Source: RGD microtubule cytoskeleton Inferred from direct assay. Source: MGI neuronal cell body Inferred from direct assay. Source: RGD plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell synapse Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular function	Rac GTPase binding Inferred from physical interaction. Source: RGD frizzled binding Inferred from Biological aspect of Ancestor. Source: RefGenome protein kinase binding Inferred from physical interaction. Source: RGD signal transducer activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 695	695	Segment polarity protein dishevelled homolog DVL-1		PRO_0000145745
Regions
	Domain	1 – 85	85	DIX
	Domain	251 – 323	73	PDZ
	Domain	425 – 499	75	DEP
Amino acid modifications
	Modified residue	559	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9WVB9 [UniParc]. Last modified March 27, 2002. Version 3. Checksum: EEC4AA99A117D22A	FASTA	695	75,447
	10 20 30 40 50 60 MAETKIIYHM DEEETPYLVK LPVAPERVTL ADFKNVLSNR PVHAYKFFFK SMDQDFGVVK 70 80 90 100 110 120 EEIFDDNAKL PCFNGRVVSW LVLAEGAHSD AGSQGTDSHT DLPPPLERTG GIGDSRPPSF 130 140 150 160 170 180 HPNVASSRDG MDNETGTESM VSHRRERARR RNRDEAARTN GHPRGDRRRE LGLPPDSAST 190 200 210 220 230 240 VLSSELESSS FIDSDEEDNT SRLSSSTEQS TSSRLIRKHK CRRRKQRLRQ TDRASSFSSI 250 260 270 280 290 300 TDSTMSLNII TVTLNMERHH FLGISIVGQS NDRGDGGIYI GSIMKGGAVA ADGRIEPGDM 310 320 330 340 350 360 LLQVNDVNFE NMSNDDAVRV LREIVSQTGP ISLTVAKCWD PTPRSYFTIP RADPVRPIDP 370 380 390 400 410 420 AAWLSHTAAL TGALPRYGTS PCSSAITRTS SSSLTSSVPG APQLEEAPLT VKSDMSAIVR 430 440 450 460 470 480 VMQLPDSGLE IRDRMWLKIT IANAVIGADV VDWLYTHVEG FKERREARKY ASSMLKHGFL 490 500 510 520 530 540 RHTVNKITFS EQCYYVFGDL CSNLASLNLN SGSSGASDQD TLAPLPHPSV PWPLGQGYPY 550 560 570 580 590 600 QYPGPPPCFP PAYQDPGFSY GSGSAGSQQS EGSKSSGSTR SSHRTPGREE RRATGAGGSG 610 620 630 640 650 660 SESDHTVPSG SGSTGWWERP VSQLSRGSSP RSQASAVAPG LPPLHPLTKA YAVVGGPPGG 670 680 690 PPVRELAAVP PELTGSRQSF QKAMGNPCEF FVDIM

References

[1]	"Mapping and sequencing rat dishevelled-1: a candidate gene for cerebral ischaemic insult in a rat model of stroke." de Lange R.P.J., Burr K., Clark J.S., Negrin C.D., Brosnan M.J., St Clair D.M., Dominiczak A.F., Shaw D.J. Neurogenetics 3:99-106(2001) [PubMed: 11354832] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: Wistar Kyoto.
[2]	"Inhibition of the Wnt signaling pathway by Idax, a novel Dvl-binding protein." Hino S., Kishida S., Michiue T., Fukui A., Sakamoto I., Takada S., Asashima M., Kikuchi A. Mol. Cell. Biol. 21:330-342(2001) [PubMed: 11113207] [Abstract] Cited for: INTERACTION WITH CXXC4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF143545 mRNA. Translation: AAD33896.2. AF143546 mRNA. Translation: AAD33897.2. AF143548, AF143547 Genomic DNA. Translation: AAD41492.2. AF143550, AF143549 Genomic DNA. Translation: AAD41493.1.
IPI	IPI00204871.
RefSeq	NP_114008.1. NM_031820.1.
UniGene	Rn.144610.
3D structure databases
ProteinModelPortal	Q9WVB9.
SMR	Q9WVB9. Positions 251-345, 406-499.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9WVB9.
Proteomic databases
PRIDE	Q9WVB9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000026439; ENSRNOP00000026439; ENSRNOG00000019423.
GeneID	83721.
KEGG	rno:83721.
UCSC	NM_031820. rat.
Organism-specific databases
CTD	1855.
RGD	620632. Dvl1.
Phylogenomic databases
eggNOG	roNOG06551.
GeneTree	ENSGT00390000013552.
HOVERGEN	HBG005542.
InParanoid	Q9WVB9.
OMA	NREGHED.
OrthoDB	EOG4640BS.
PhylomeDB	Q9WVB9.
Gene expression databases
ArrayExpress	Q9WVB9.
Genevestigator	Q9WVB9.
GermOnline	ENSRNOG00000019423. Rattus norvegicus.
Family and domain databases
InterPro	IPR000591. DEP_dom. IPR008339. Dishevelled. IPR008340. Dishevelled_1. IPR024580. Dishevelled_C-dom. IPR003351. Dishevelled_protein_dom. IPR001158. DIX. IPR015506. Dsh. IPR001478. PDZ/DHR/GLGF. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K02353.
PANTHER	PTHR10878:SF5. Dishevelled_1. 1 hit. PTHR10878. Dsh. 1 hit.
Pfam	PF00610. DEP. 1 hit. PF02377. Dishevelled. 1 hit. PF00778. DIX. 1 hit. PF12316. Dsh_C. 1 hit. PF00595. PDZ. 1 hit. [Graphical view]
PRINTS	PR01760. DISHEVELLED. PR01761. DISHEVELLED1.
SMART	SM00021. DAX. 1 hit. SM00049. DEP. 1 hit. SM00228. PDZ. 1 hit. [Graphical view]
SUPFAM	SSF50156. PDZ. 1 hit.
PROSITE	PS50186. DEP. 1 hit. PS50841. DIX. 1 hit. PS50106. PDZ. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	616297.

Entry information

Entry name

DVL1_RAT

Accession

Primary (citable) accession number: Q9WVB9
Secondary accession number(s): Q9QUG5, Q9WVB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	March 27, 2002
Last modified:	January 25, 2012
This is version 100 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program