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Protein

Transducin-like enhancer protein 6

Gene

Tle6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

As a member of the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions.1 Publication

GO - Biological processi

  • positive regulation of neurogenesis Source: MGI
  • positive regulation of neuron differentiation Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Transducin-like enhancer protein 6
Alternative name(s):
Groucho-related protein 6
Gene namesi
Name:Tle6
Synonyms:Grg6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:2149593. Tle6.

Subcellular locationi

  • Cytoplasm

  • Note: In the subcortical cytoplasm of early embryos from the 1-cell to the blastocyst stages. From the 2-cell stage, still detected in the subcortex, but excluded from cell-cell contact regions.

GO - Cellular componenti

  • cell cortex Source: MGI
  • cytosol Source: MGI
  • nucleus Source: MGI
  • protein complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 581581Transducin-like enhancer protein 6PRO_0000051287Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei139 – 1391PhosphoserineSequence analysis
Modified residuei187 – 1871PhosphoserineSequence analysis
Modified residuei209 – 2091PhosphoserineSequence analysis

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9WVB3.
PaxDbiQ9WVB3.
PRIDEiQ9WVB3.

PTM databases

iPTMnetiQ9WVB3.
PhosphoSiteiQ9WVB3.

Expressioni

Tissue specificityi

Expressed predominantly in ovaries, where it is restricted to growing oocytes, with greatest levels in fully grown oocytes (PubMed:18804437). Expressed predominantly in testis, heart, lung, liver and muscle, and at very low levels in the brain, spleen and kidney (PubMed:11486032).2 Publications

Developmental stagei

Transcripts first detected at 15.5 dpc and peak 1 week after birth. Transcripts accumulate during oogenesis. During meiotic maturation, the vast majority of the transcripts are degraded and virtually none is detected by 2-cell stage embryogenesis. The protein however persists during preimplantation up to the blastocyst stage. At 2-cell stage, excluded from cell-cell contact regions. Continuous exclusion from these regions during preimplantation development leads to the absence of the protein from the inner cells of the morula and the inner cell mass of the blastocyst.1 Publication

Inductioni

By E2A-HLF, a chimeric transcription factor containing the transactivation domain of E2A linked to the DNA-binding and dimerization domain of HLF.

Gene expression databases

BgeeiQ9WVB3.
ExpressionAtlasiQ9WVB3. baseline and differential.
GenevisibleiQ9WVB3. MM.

Interactioni

Subunit structurei

Component of the SCMC which also includes KHDC3, OOEP and NLRP5.1 Publication

Protein-protein interaction databases

BioGridi227763. 20 interactions.
IntActiQ9WVB3. 3 interactions.
STRINGi10090.ENSMUSP00000071905.

Structurei

3D structure databases

ProteinModelPortaliQ9WVB3.
SMRiQ9WVB3. Positions 269-581.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati246 – 28338WD 1Add
BLAST
Repeati291 – 32939WD 2Add
BLAST
Repeati341 – 38141WD 3Add
BLAST
Repeati384 – 42441WD 4Add
BLAST
Repeati427 – 46438WD 5Add
BLAST
Repeati466 – 50540WD 6Add
BLAST
Repeati508 – 54740WD 7Add
BLAST
Repeati549 – 58032WD 8Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi132 – 1387Nuclear localization signalSequence analysis

Domaini

Contrary to other WD repeat Groucho/TLE family members, does not contain any identifiable Q, GP, CcN or SP domains. Only the C-terminal WD-repeat domain is conserved.1 Publication

Sequence similaritiesi

Belongs to the WD repeat Groucho/TLE family.Curated
Contains 8 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0639. Eukaryota.
ENOG410XPX3. LUCA.
GeneTreeiENSGT00550000074465.
HOGENOMiHOG000154599.
HOVERGENiHBG014064.
InParanoidiQ9WVB3.
OMAiTEDWLLL.
PhylomeDBiQ9WVB3.
TreeFamiTF314167.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WVB3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSHRQSSDT FGGILPSTLS SRYLSIVNQL PEEFSSVVSE MMVHLENIFS
60 70 80 90 100
LAENFFQAIE RFSRTPDLLE RNKMSIGVGA EGDSWPCHVS HEAPMGSAQT
110 120 130 140 150
TENSAKEEDK QVPESAALQH PKFKSTPGPQ LPTRRRFLSE SDELQDPQPV
160 170 180 190 200
WDAEPQFCQG FLIQGLWELF MDSRQKNQQE HGGEDSSQES KDSGLCDFKP
210 220 230 240 250
EPQPRHRNSL SDSADPFLIK SPSALLDYYQ EDVSRPQPET QESSGRADKF
260 270 280 290 300
LKPLSWGSEV LESSCNQPST ALWQLERFTV PQALQKVRVL KHQELLLVVA
310 320 330 340 350
VSSFTRHVFT CSQSGIKVWN LVNQVAEDRD PESHLKCSVQ DNKVYLRTCL
360 370 380 390 400
LSSNSRTLFA GGYNLPGVIV WDLAAPSLYE KCQLPCEGLS CQALANTKEN
410 420 430 440 450
MALAGFTDGT VRIWDLRTQE IVRNLKGPTN SARNLVVKDD NIWTGGLDAC
460 470 480 490 500
LRCWDLRMAK VSLEHLFQSQ IMSLAHSPTE DWLLLGLANG QHCLFNSRKR
510 520 530 540 550
DQVLTVDTKD NTILGLKFSP NGKWWASVGM GNFITVHSMP TGAKLFQVPE
560 570 580
VGPVRCFDMT ENGRLIITGS RDCASVYHIK Y
Length:581
Mass (Da):65,116
Last modified:November 1, 1999 - v1
Checksum:i946369E084B800F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF145957 mRNA. Translation: AAD37693.1.
CCDSiCCDS24064.1.
RefSeqiNP_444484.1. NM_053254.2.
UniGeneiMm.206764.

Genome annotation databases

EnsembliENSMUST00000072020; ENSMUSP00000071905; ENSMUSG00000034758.
GeneIDi114606.
KEGGimmu:114606.
UCSCiuc007giu.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF145957 mRNA. Translation: AAD37693.1.
CCDSiCCDS24064.1.
RefSeqiNP_444484.1. NM_053254.2.
UniGeneiMm.206764.

3D structure databases

ProteinModelPortaliQ9WVB3.
SMRiQ9WVB3. Positions 269-581.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi227763. 20 interactions.
IntActiQ9WVB3. 3 interactions.
STRINGi10090.ENSMUSP00000071905.

PTM databases

iPTMnetiQ9WVB3.
PhosphoSiteiQ9WVB3.

Proteomic databases

MaxQBiQ9WVB3.
PaxDbiQ9WVB3.
PRIDEiQ9WVB3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000072020; ENSMUSP00000071905; ENSMUSG00000034758.
GeneIDi114606.
KEGGimmu:114606.
UCSCiuc007giu.1. mouse.

Organism-specific databases

CTDi79816.
MGIiMGI:2149593. Tle6.

Phylogenomic databases

eggNOGiKOG0639. Eukaryota.
ENOG410XPX3. LUCA.
GeneTreeiENSGT00550000074465.
HOGENOMiHOG000154599.
HOVERGENiHBG014064.
InParanoidiQ9WVB3.
OMAiTEDWLLL.
PhylomeDBiQ9WVB3.
TreeFamiTF314167.

Miscellaneous databases

PROiQ9WVB3.
SOURCEiSearch...

Gene expression databases

BgeeiQ9WVB3.
ExpressionAtlasiQ9WVB3. baseline and differential.
GenevisibleiQ9WVB3. MM.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 1 hit.
PS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The E2A-HLF oncoprotein activates Groucho-related genes and suppresses Runx1."
    Dang J., Inukai T., Kurosawa H., Goi K., Inaba T., Lenny N.T., Downing J.R., Stifani S., Look A.T.
    Mol. Cell. Biol. 21:5935-5945(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Premonocytic lymphoma.
  2. "A subcortical maternal complex essential for preimplantation mouse embryogenesis."
    Li L., Baibakov B., Dean J.
    Dev. Cell 15:416-425(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, IDENTIFICATION IN THE SCMC COMPLEX WITH KHDC3; NLRP5 AND OOEP, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  3. "The Groucho/TLE/Grg family of transcriptional co-repressors."
    Jennings B.H., Ish-Horowicz D.
    Genome Biol. 9:R205.1-R205.7(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiTLE6_MOUSE
AccessioniPrimary (citable) accession number: Q9WVB3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: November 1, 1999
Last modified: June 8, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.