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Protein

Mitotic checkpoint protein BUB3

Gene

Bub3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1 (By similarity). Regulates chromosome segregation during oocyte meiosis.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • attachment of spindle microtubules to kinetochore Source: MGI
  • cell division Source: UniProtKB-KW
  • chromosome segregation Source: MGI
  • meiotic cell cycle Source: UniProtKB-KW
  • mitotic sister chromatid segregation Source: MGI
  • mitotic spindle assembly checkpoint Source: GO_Central
  • regulation of chromosome segregation Source: UniProtKB
  • spindle assembly checkpoint Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Chromosome partition, Meiosis, Mitosis

Enzyme and pathway databases

ReactomeiR-MMU-141430. Inactivation of APC/C via direct inhibition of the APC/C complex.
R-MMU-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-MMU-176409. APC/C:Cdc20 mediated degradation of mitotic proteins.
R-MMU-179409. APC-Cdc20 mediated degradation of Nek2A.
R-MMU-2467813. Separation of Sister Chromatids.
R-MMU-2500257. Resolution of Sister Chromatid Cohesion.
R-MMU-5663220. RHO GTPases Activate Formins.
R-MMU-68877. Mitotic Prometaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitotic checkpoint protein BUB3
Alternative name(s):
WD repeat type I transmembrane protein A72.5
Gene namesi
Name:Bub3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1343463. Bub3.

Subcellular locationi

  • Nucleus By similarity
  • Chromosomecentromerekinetochore 1 Publication

  • Note: Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000508921 – 326Mitotic checkpoint protein BUB3Add BLAST326

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei179N6-acetyllysineBy similarity1
Modified residuei211PhosphoserineBy similarity1
Cross-linki216Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Poly-ADP-ribosylated by PARP1.

Keywords - PTMi

Acetylation, ADP-ribosylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9WVA3.
MaxQBiQ9WVA3.
PaxDbiQ9WVA3.
PeptideAtlasiQ9WVA3.
PRIDEiQ9WVA3.

2D gel databases

REPRODUCTION-2DPAGEQ9WVA3.

PTM databases

iPTMnetiQ9WVA3.
PhosphoSitePlusiQ9WVA3.
SwissPalmiQ9WVA3.

Expressioni

Gene expression databases

BgeeiENSMUSG00000066979.
ExpressionAtlasiQ9WVA3. baseline and differential.
GenevisibleiQ9WVA3. MM.

Interactioni

Subunit structurei

Interacts with BUB1 and BUBR1. The BUB1/BUB3 complex interacts with MAD1L1. Interacts with ZNF207/BuGZ; leading to promote stability and kinetochore loading of BUB3 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198406. 2 interactors.
IntActiQ9WVA3. 3 interactors.
MINTiMINT-237495.
STRINGi10090.ENSMUSP00000081547.

Structurei

3D structure databases

ProteinModelPortaliQ9WVA3.
SMRiQ9WVA3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati5 – 43WD 1Add BLAST39
Repeati46 – 83WD 2Add BLAST38
Repeati86 – 124WD 3Add BLAST39
Repeati128 – 163WD 4Add BLAST36
Repeati223 – 262WD 5Add BLAST40

Sequence similaritiesi

Belongs to the WD repeat BUB3 family.Curated
Contains 5 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1036. Eukaryota.
ENOG410XRBG. LUCA.
GeneTreeiENSGT00530000063440.
HOGENOMiHOG000208822.
HOVERGENiHBG002942.
InParanoidiQ9WVA3.
KOiK02180.
OMAiEGEKSHE.
OrthoDBiEOG091G0AB8.
TreeFamiTF105454.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 3 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WVA3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTGSNEFKLN QPPEDGISSV KFSPNTSQFL LVSSWDTSVR LYDVPANSMR
60 70 80 90 100
LKYQHTGAVL DCAFYDPTHA WSGGLDHQLK MHDLNTDQEN LVGTHDAPIR
110 120 130 140 150
CVEYCPEVNV MVTGSWDQTV KLWDPRTPCN AGTFSQPEKV YTLSVSGDRL
160 170 180 190 200
IVGTAGRRVL VWDLRNMGYV QQRRESSLKY QTRCIRAFPN KQGYVLSSIE
210 220 230 240 250
GRVAVEYLDP SPEVQKKKYA FKCHRLKENN IEQIYPVNAI SFHNIHNTFA
260 270 280 290 300
TGGSDGFVNI WDPFNKKRLC QFHRYPTSIA SLAFSNDGTT LAIASSYMYE
310 320
MDDTEHPEDG IFIRQVTDAE TKPKST
Length:326
Mass (Da):36,955
Last modified:July 27, 2011 - v2
Checksum:iF1EA57368E5A6345
GO

Sequence cautioni

The sequence AAB39606 differs from that shown. Reason: Frameshift at positions 73, 270 and 274.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti62C → W in AAB39606 (Ref. 2) Curated1
Sequence conflicti156G → A in AAB39606 (Ref. 2) Curated1
Sequence conflicti165R → W in AAD38038 (PubMed:10411903).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF149822 mRNA. Translation: AAD38038.1.
U67327 mRNA. Translation: AAB39606.1. Frameshift.
AK088534 mRNA. Translation: BAC40409.1.
BC025089 mRNA. Translation: AAH25089.1.
CCDSiCCDS40160.1.
RefSeqiNP_001304279.1. NM_001317350.1.
NP_033904.2. NM_009774.4.
UniGeneiMm.441749.
Mm.927.

Genome annotation databases

EnsembliENSMUST00000084502; ENSMUSP00000081547; ENSMUSG00000066979.
GeneIDi12237.
KEGGimmu:12237.
UCSCiuc009kbq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF149822 mRNA. Translation: AAD38038.1.
U67327 mRNA. Translation: AAB39606.1. Frameshift.
AK088534 mRNA. Translation: BAC40409.1.
BC025089 mRNA. Translation: AAH25089.1.
CCDSiCCDS40160.1.
RefSeqiNP_001304279.1. NM_001317350.1.
NP_033904.2. NM_009774.4.
UniGeneiMm.441749.
Mm.927.

3D structure databases

ProteinModelPortaliQ9WVA3.
SMRiQ9WVA3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198406. 2 interactors.
IntActiQ9WVA3. 3 interactors.
MINTiMINT-237495.
STRINGi10090.ENSMUSP00000081547.

PTM databases

iPTMnetiQ9WVA3.
PhosphoSitePlusiQ9WVA3.
SwissPalmiQ9WVA3.

2D gel databases

REPRODUCTION-2DPAGEQ9WVA3.

Proteomic databases

EPDiQ9WVA3.
MaxQBiQ9WVA3.
PaxDbiQ9WVA3.
PeptideAtlasiQ9WVA3.
PRIDEiQ9WVA3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000084502; ENSMUSP00000081547; ENSMUSG00000066979.
GeneIDi12237.
KEGGimmu:12237.
UCSCiuc009kbq.1. mouse.

Organism-specific databases

CTDi9184.
MGIiMGI:1343463. Bub3.

Phylogenomic databases

eggNOGiKOG1036. Eukaryota.
ENOG410XRBG. LUCA.
GeneTreeiENSGT00530000063440.
HOGENOMiHOG000208822.
HOVERGENiHBG002942.
InParanoidiQ9WVA3.
KOiK02180.
OMAiEGEKSHE.
OrthoDBiEOG091G0AB8.
TreeFamiTF105454.

Enzyme and pathway databases

ReactomeiR-MMU-141430. Inactivation of APC/C via direct inhibition of the APC/C complex.
R-MMU-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-MMU-176409. APC/C:Cdc20 mediated degradation of mitotic proteins.
R-MMU-179409. APC-Cdc20 mediated degradation of Nek2A.
R-MMU-2467813. Separation of Sister Chromatids.
R-MMU-2500257. Resolution of Sister Chromatid Cohesion.
R-MMU-5663220. RHO GTPases Activate Formins.
R-MMU-68877. Mitotic Prometaphase.

Miscellaneous databases

PROiQ9WVA3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000066979.
ExpressionAtlasiQ9WVA3. baseline and differential.
GenevisibleiQ9WVA3. MM.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 3 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 2 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBUB3_MOUSE
AccessioniPrimary (citable) accession number: Q9WVA3
Secondary accession number(s): P97397, Q6ZWM5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.