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Protein

Mitotic checkpoint protein BUB3

Gene

Bub3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1 (By similarity). Regulates chromosome segregation during oocyte meiosis.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • attachment of spindle microtubules to kinetochore Source: MGI
  • cell division Source: UniProtKB-KW
  • chromosome segregation Source: MGI
  • meiotic cell cycle Source: UniProtKB-KW
  • mitotic sister chromatid segregation Source: MGI
  • mitotic spindle assembly checkpoint Source: GO_Central
  • regulation of chromosome segregation Source: UniProtKB

Keywordsi

Biological processCell cycle, Cell division, Chromosome partition, Meiosis, Mitosis

Enzyme and pathway databases

ReactomeiR-MMU-141430 Inactivation of APC/C via direct inhibition of the APC/C complex
R-MMU-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-MMU-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-MMU-176409 APC/C:Cdc20 mediated degradation of mitotic proteins
R-MMU-179409 APC-Cdc20 mediated degradation of Nek2A
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2500257 Resolution of Sister Chromatid Cohesion
R-MMU-5663220 RHO GTPases Activate Formins
R-MMU-68877 Mitotic Prometaphase

Names & Taxonomyi

Protein namesi
Recommended name:
Mitotic checkpoint protein BUB3
Alternative name(s):
WD repeat type I transmembrane protein A72.5
Gene namesi
Name:Bub3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1343463 Bub3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000508921 – 326Mitotic checkpoint protein BUB3Add BLAST326

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei179N6-acetyllysineBy similarity1
Modified residuei211PhosphoserineBy similarity1
Cross-linki216Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Poly-ADP-ribosylated by PARP1.

Keywords - PTMi

Acetylation, ADP-ribosylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9WVA3
MaxQBiQ9WVA3
PaxDbiQ9WVA3
PeptideAtlasiQ9WVA3
PRIDEiQ9WVA3

2D gel databases

REPRODUCTION-2DPAGEiQ9WVA3

PTM databases

iPTMnetiQ9WVA3
PhosphoSitePlusiQ9WVA3
SwissPalmiQ9WVA3

Expressioni

Gene expression databases

BgeeiENSMUSG00000066979
ExpressionAtlasiQ9WVA3 baseline and differential
GenevisibleiQ9WVA3 MM

Interactioni

Subunit structurei

Interacts with BUB1 and BUBR1. The BUB1/BUB3 complex interacts with MAD1L1. Interacts with ZNF207/BuGZ; leading to promote stability and kinetochore loading of BUB3 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198406, 3 interactors
IntActiQ9WVA3, 4 interactors
MINTiQ9WVA3
STRINGi10090.ENSMUSP00000081547

Structurei

3D structure databases

ProteinModelPortaliQ9WVA3
SMRiQ9WVA3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati5 – 43WD 1Add BLAST39
Repeati46 – 83WD 2Add BLAST38
Repeati86 – 124WD 3Add BLAST39
Repeati128 – 163WD 4Add BLAST36
Repeati223 – 262WD 5Add BLAST40

Sequence similaritiesi

Belongs to the WD repeat BUB3 family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1036 Eukaryota
ENOG410XRBG LUCA
GeneTreeiENSGT00530000063440
HOGENOMiHOG000208822
HOVERGENiHBG002942
InParanoidiQ9WVA3
KOiK02180
OMAiCMPNDAG
OrthoDBiEOG091G0AB8
TreeFamiTF105454

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR020472 G-protein_beta_WD-40_rep
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF00400 WD40, 3 hits
PRINTSiPR00320 GPROTEINBRPT
SMARTiView protein in SMART
SM00320 WD40, 6 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS50082 WD_REPEATS_2, 2 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

Q9WVA3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTGSNEFKLN QPPEDGISSV KFSPNTSQFL LVSSWDTSVR LYDVPANSMR
60 70 80 90 100
LKYQHTGAVL DCAFYDPTHA WSGGLDHQLK MHDLNTDQEN LVGTHDAPIR
110 120 130 140 150
CVEYCPEVNV MVTGSWDQTV KLWDPRTPCN AGTFSQPEKV YTLSVSGDRL
160 170 180 190 200
IVGTAGRRVL VWDLRNMGYV QQRRESSLKY QTRCIRAFPN KQGYVLSSIE
210 220 230 240 250
GRVAVEYLDP SPEVQKKKYA FKCHRLKENN IEQIYPVNAI SFHNIHNTFA
260 270 280 290 300
TGGSDGFVNI WDPFNKKRLC QFHRYPTSIA SLAFSNDGTT LAIASSYMYE
310 320
MDDTEHPEDG IFIRQVTDAE TKPKST
Length:326
Mass (Da):36,955
Last modified:July 27, 2011 - v2
Checksum:iF1EA57368E5A6345
GO

Sequence cautioni

The sequence AAB39606 differs from that shown. Reason: Frameshift at positions 73, 270 and 274.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti62C → W in AAB39606 (Ref. 2) Curated1
Sequence conflicti156G → A in AAB39606 (Ref. 2) Curated1
Sequence conflicti165R → W in AAD38038 (PubMed:10411903).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF149822 mRNA Translation: AAD38038.1
U67327 mRNA Translation: AAB39606.1 Frameshift.
AK088534 mRNA Translation: BAC40409.1
BC025089 mRNA Translation: AAH25089.1
CCDSiCCDS40160.1
RefSeqiNP_001304279.1, NM_001317350.1
NP_033904.2, NM_009774.4
UniGeneiMm.441749
Mm.927

Genome annotation databases

EnsembliENSMUST00000084502; ENSMUSP00000081547; ENSMUSG00000066979
GeneIDi12237
KEGGimmu:12237
UCSCiuc009kbq.1 mouse

Similar proteinsi

Entry informationi

Entry nameiBUB3_MOUSE
AccessioniPrimary (citable) accession number: Q9WVA3
Secondary accession number(s): P97397, Q6ZWM5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 27, 2011
Last modified: March 28, 2018
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health