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Protein

Mitochondrial import inner membrane translocase subunit Tim8 A

Gene

Timm8a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space. The TIMM8-TIMM13 complex mediates the import of proteins such as TIMM23, SLC25A12/ARALAR1 and SLC25A13/ARALAR2, while the predominant TIMM9-TIMM10 70 kDa complex mediates the import of much more proteins (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Protein transport, Translocation, Transport

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit Tim8 A
Alternative name(s):
Deafness dystonia protein 1 homolog
Gene namesi
Name:Timm8a1
Synonyms:Ddp1, Tim8a, Timm8a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componentsi: Chromosome 10, Chromosome X

Organism-specific databases

MGIiMGI:1353433. Timm8a1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001935851 – 97Mitochondrial import inner membrane translocase subunit Tim8 AAdd BLAST97

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi43 ↔ 66By similarity
Disulfide bondi47 ↔ 62By similarity
Modified residuei57PhosphoserineCombined sources1
Modified residuei87PhosphoserineCombined sources1
Modified residuei94PhosphoserineCombined sources1
Modified residuei96PhosphoserineCombined sources1

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

EPDiQ9WVA2.
MaxQBiQ9WVA2.
PaxDbiQ9WVA2.
PeptideAtlasiQ9WVA2.
PRIDEiQ9WVA2.

PTM databases

iPTMnetiQ9WVA2.
PhosphoSitePlusiQ9WVA2.

Expressioni

Tissue specificityi

Present at high level in liver and brain, and at lower level in muscle and heart. In CNS sections, it is predominantly present in the soma and the dendritic portion of the Purkinje cells of the cerebellum, but not in the glial cells. Scattered expression also is also detected in the brain stem, olfactory bulb, substantia nigra, hippocampus and striatum (at protein level). Ubiquitously expressed.2 Publications

Gene expression databases

BgeeiENSMUSG00000045455.
GenevisibleiQ9WVA2. MM.

Interactioni

Subunit structurei

Heterohexamer; composed of 3 copies of TIMM8A and 3 copies of TIMM13, named soluble 70 kDa complex. Associates with the TIM22 complex, whose core is composed of TIMM22 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ9WVA2. 2 interactors.
MINTiMINT-4137932.
STRINGi10090.ENSMUSP00000050156.

Structurei

3D structure databases

ProteinModelPortaliQ9WVA2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi43 – 66Twin CX3C motifAdd BLAST24

Domaini

The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space. However, during the transit of TIMM8A from cytoplasm into mitochondrion, the Cys residues probably coordinate zinc, thereby preventing folding and allowing its transfer across mitochondrial outer membrane (By similarity).By similarity

Sequence similaritiesi

Belongs to the small Tim family.Curated

Phylogenomic databases

eggNOGiKOG3489. Eukaryota.
ENOG41126D5. LUCA.
GeneTreeiENSGT00390000016102.
HOGENOMiHOG000115758.
HOVERGENiHBG060492.
InParanoidiQ9WVA2.
KOiK17780.
OMAiFTEMCWD.
OrthoDBiEOG091G11XE.
PhylomeDBiQ9WVA2.
TreeFamiTF106191.

Family and domain databases

Gene3Di1.10.287.810. 1 hit.
InterProiIPR004217. Tim10/DDP_fam_Znf.
[Graphical view]
PfamiPF02953. zf-Tim10_DDP. 1 hit.
[Graphical view]
SUPFAMiSSF144122. SSF144122. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9WVA2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESSTSSSGS ALGAVDPQLQ HFIEVETQKQ RFQQLVHQMT ELCWEKCMDK
60 70 80 90
PGPKLDSRAE ACFVNCVERF IDTSQFILNR LEQTQKSKPV FSESLSD
Length:97
Mass (Da):11,042
Last modified:November 1, 1999 - v1
Checksum:i16C23D7C351BDF00
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF150081 mRNA. Translation: AAD39988.1.
AB031055 mRNA. Translation: BAA90770.1.
AK011402 mRNA. Translation: BAB27594.1.
AK088903 mRNA. Translation: BAC40644.1.
AK090079 mRNA. Translation: BAC41082.1.
AK165573 mRNA. Translation: BAE38265.1.
BC004796 mRNA. Translation: AAH04796.1.
BC094631 mRNA. Translation: AAH94631.1.
CCDSiCCDS41122.1.
PIRiJC7322.
RefSeqiNP_038926.1. NM_013898.3.
UniGeneiMm.214504.

Genome annotation databases

EnsembliENSMUST00000052902; ENSMUSP00000049749; ENSMUSG00000045455.
ENSMUST00000054213; ENSMUSP00000050156; ENSMUSG00000048007.
GeneIDi30058.
KEGGimmu:30058.
UCSCiuc009ugd.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF150081 mRNA. Translation: AAD39988.1.
AB031055 mRNA. Translation: BAA90770.1.
AK011402 mRNA. Translation: BAB27594.1.
AK088903 mRNA. Translation: BAC40644.1.
AK090079 mRNA. Translation: BAC41082.1.
AK165573 mRNA. Translation: BAE38265.1.
BC004796 mRNA. Translation: AAH04796.1.
BC094631 mRNA. Translation: AAH94631.1.
CCDSiCCDS41122.1.
PIRiJC7322.
RefSeqiNP_038926.1. NM_013898.3.
UniGeneiMm.214504.

3D structure databases

ProteinModelPortaliQ9WVA2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9WVA2. 2 interactors.
MINTiMINT-4137932.
STRINGi10090.ENSMUSP00000050156.

PTM databases

iPTMnetiQ9WVA2.
PhosphoSitePlusiQ9WVA2.

Proteomic databases

EPDiQ9WVA2.
MaxQBiQ9WVA2.
PaxDbiQ9WVA2.
PeptideAtlasiQ9WVA2.
PRIDEiQ9WVA2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000052902; ENSMUSP00000049749; ENSMUSG00000045455.
ENSMUST00000054213; ENSMUSP00000050156; ENSMUSG00000048007.
GeneIDi30058.
KEGGimmu:30058.
UCSCiuc009ugd.1. mouse.

Organism-specific databases

CTDi30058.
MGIiMGI:1353433. Timm8a1.

Phylogenomic databases

eggNOGiKOG3489. Eukaryota.
ENOG41126D5. LUCA.
GeneTreeiENSGT00390000016102.
HOGENOMiHOG000115758.
HOVERGENiHBG060492.
InParanoidiQ9WVA2.
KOiK17780.
OMAiFTEMCWD.
OrthoDBiEOG091G11XE.
PhylomeDBiQ9WVA2.
TreeFamiTF106191.

Miscellaneous databases

PROiQ9WVA2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000045455.
GenevisibleiQ9WVA2. MM.

Family and domain databases

Gene3Di1.10.287.810. 1 hit.
InterProiIPR004217. Tim10/DDP_fam_Znf.
[Graphical view]
PfamiPF02953. zf-Tim10_DDP. 1 hit.
[Graphical view]
SUPFAMiSSF144122. SSF144122. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTIM8A_MOUSE
AccessioniPrimary (citable) accession number: Q9WVA2
Secondary accession number(s): Q542E5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1999
Last modified: November 2, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.