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Q9WVA2

- TIM8A_MOUSE

UniProt

Q9WVA2 - TIM8A_MOUSE

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Protein

Mitochondrial import inner membrane translocase subunit Tim8 A

Gene
Timm8a1, Ddp1, Tim8a, Timm8a
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space. The TIMM8-TIMM13 complex mediates the import of proteins such as TIMM23, SLC25A12/ARALAR1 and SLC25A13/ARALAR2, while the predominant TIMM9-TIMM10 70 kDa complex mediates the import of much more proteins By similarity.

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Protein transport, Translocation, Transport

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_199101. Mitochondrial protein import.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit Tim8 A
Alternative name(s):
Deafness dystonia protein 1 homolog
Gene namesi
Name:Timm8a1
Synonyms:Ddp1, Tim8a, Timm8a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10, UP000000589: Chromosome X

Organism-specific databases

MGIiMGI:1353433. Timm8a1.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial inner membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 9797Mitochondrial import inner membrane translocase subunit Tim8 APRO_0000193585Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi43 ↔ 66 By similarity
Disulfide bondi47 ↔ 62 By similarity
Modified residuei87 – 871Phosphoserine1 Publication
Modified residuei94 – 941Phosphoserine By similarity
Modified residuei96 – 961Phosphoserine1 Publication

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

MaxQBiQ9WVA2.
PaxDbiQ9WVA2.
PRIDEiQ9WVA2.

PTM databases

PhosphoSiteiQ9WVA2.

Expressioni

Tissue specificityi

Present at high level in liver and brain, and at lower level in muscle and heart. In CNS sections, it is predominantly present in the soma and the dendritic portion of the Purkinje cells of the cerebellum, but not in the glial cells. Scattered expression also is also detected in the brain stem, olfactory bulb, substantia nigra, hippocampus and striatum (at protein level). Ubiquitously expressed.2 Publications

Gene expression databases

BgeeiQ9WVA2.
GenevestigatoriQ9WVA2.

Interactioni

Subunit structurei

Heterohexamer; composed of 3 copies of TIMM8A and 3 copies of TIMM13, named soluble 70 kDa complex. Associates with the TIM22 complex, whose core is composed of TIMM22 By similarity.

Protein-protein interaction databases

IntActiQ9WVA2. 2 interactions.
MINTiMINT-4137932.

Structurei

3D structure databases

ProteinModelPortaliQ9WVA2.
SMRiQ9WVA2. Positions 29-84.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi43 – 6624Twin CX3C motifAdd
BLAST

Domaini

The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space. However, during the transit of TIMM8A from cytoplasm into mitochondrion, the Cys residues probably coordinate zinc, thereby preventing folding and allowing its transfer across mitochondrial outer membrane By similarity.

Sequence similaritiesi

Belongs to the small Tim family.

Phylogenomic databases

eggNOGiNOG238740.
GeneTreeiENSGT00390000016102.
HOGENOMiHOG000115758.
HOVERGENiHBG060492.
InParanoidiQ9WVA2.
KOiK17780.
OMAiDRSEEPC.
OrthoDBiEOG79W97P.
PhylomeDBiQ9WVA2.
TreeFamiTF106191.

Family and domain databases

Gene3Di1.10.287.810. 1 hit.
InterProiIPR004217. Tim10/DDP_fam_Znf.
[Graphical view]
PfamiPF02953. zf-Tim10_DDP. 1 hit.
[Graphical view]
SUPFAMiSSF144122. SSF144122. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9WVA2-1 [UniParc]FASTAAdd to Basket

« Hide

MESSTSSSGS ALGAVDPQLQ HFIEVETQKQ RFQQLVHQMT ELCWEKCMDK   50
PGPKLDSRAE ACFVNCVERF IDTSQFILNR LEQTQKSKPV FSESLSD 97
Length:97
Mass (Da):11,042
Last modified:November 1, 1999 - v1
Checksum:i16C23D7C351BDF00
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF150081 mRNA. Translation: AAD39988.1.
AB031055 mRNA. Translation: BAA90770.1.
AK011402 mRNA. Translation: BAB27594.1.
AK088903 mRNA. Translation: BAC40644.1.
AK090079 mRNA. Translation: BAC41082.1.
AK165573 mRNA. Translation: BAE38265.1.
BC004796 mRNA. Translation: AAH04796.1.
BC094631 mRNA. Translation: AAH94631.1.
CCDSiCCDS41122.1.
PIRiJC7322.
RefSeqiNP_038926.1. NM_013898.2.
UniGeneiMm.214504.

Genome annotation databases

EnsembliENSMUST00000052902; ENSMUSP00000049749; ENSMUSG00000045455.
ENSMUST00000054213; ENSMUSP00000050156; ENSMUSG00000048007.
GeneIDi30058.
KEGGimmu:30058.
UCSCiuc009ugd.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF150081 mRNA. Translation: AAD39988.1 .
AB031055 mRNA. Translation: BAA90770.1 .
AK011402 mRNA. Translation: BAB27594.1 .
AK088903 mRNA. Translation: BAC40644.1 .
AK090079 mRNA. Translation: BAC41082.1 .
AK165573 mRNA. Translation: BAE38265.1 .
BC004796 mRNA. Translation: AAH04796.1 .
BC094631 mRNA. Translation: AAH94631.1 .
CCDSi CCDS41122.1.
PIRi JC7322.
RefSeqi NP_038926.1. NM_013898.2.
UniGenei Mm.214504.

3D structure databases

ProteinModelPortali Q9WVA2.
SMRi Q9WVA2. Positions 29-84.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9WVA2. 2 interactions.
MINTi MINT-4137932.

PTM databases

PhosphoSitei Q9WVA2.

Proteomic databases

MaxQBi Q9WVA2.
PaxDbi Q9WVA2.
PRIDEi Q9WVA2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000052902 ; ENSMUSP00000049749 ; ENSMUSG00000045455 .
ENSMUST00000054213 ; ENSMUSP00000050156 ; ENSMUSG00000048007 .
GeneIDi 30058.
KEGGi mmu:30058.
UCSCi uc009ugd.1. mouse.

Organism-specific databases

CTDi 30058.
MGIi MGI:1353433. Timm8a1.

Phylogenomic databases

eggNOGi NOG238740.
GeneTreei ENSGT00390000016102.
HOGENOMi HOG000115758.
HOVERGENi HBG060492.
InParanoidi Q9WVA2.
KOi K17780.
OMAi DRSEEPC.
OrthoDBi EOG79W97P.
PhylomeDBi Q9WVA2.
TreeFami TF106191.

Enzyme and pathway databases

Reactomei REACT_199101. Mitochondrial protein import.

Miscellaneous databases

NextBioi 307190.
PROi Q9WVA2.
SOURCEi Search...

Gene expression databases

Bgeei Q9WVA2.
Genevestigatori Q9WVA2.

Family and domain databases

Gene3Di 1.10.287.810. 1 hit.
InterProi IPR004217. Tim10/DDP_fam_Znf.
[Graphical view ]
Pfami PF02953. zf-Tim10_DDP. 1 hit.
[Graphical view ]
SUPFAMi SSF144122. SSF144122. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The mitochondrial TIM22 preprotein translocase is highly conserved throughout the eukaryotic kingdom."
    Bauer M.F., Rothbauer U., Muehlenbein N., Smith R.J.H., Gerbitz K.-D., Neupert W., Brunner M., Hofmann S.
    FEBS Lett. 464:41-47(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning and expression of mouse deafness dystonia peptide 1 cDNA."
    Nakane T., Inada Y., Ito F., Itoh N., Tazawa S., Chiba S.
    Biochem. Biophys. Res. Commun. 273:759-764(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Adipocyte.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Submandibular gland.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and Czech II.
    Tissue: Mammary gland and Thymus.
  5. "The calcium-binding aspartate/glutamate carriers, citrin and aralar1, are new substrates for the DDP1/TIMM8a-TIMM13 complex."
    Roesch K., Hynds P.J., Varga R., Tranebjaerg L., Koehler C.M.
    Hum. Mol. Genet. 13:2101-2111(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-87 AND SER-96, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiTIM8A_MOUSE
AccessioniPrimary (citable) accession number: Q9WVA2
Secondary accession number(s): Q542E5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1999
Last modified: September 3, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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