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Q9WVA2

- TIM8A_MOUSE

UniProt

Q9WVA2 - TIM8A_MOUSE

Protein

Mitochondrial import inner membrane translocase subunit Tim8 A

Gene

Timm8a1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space. The TIMM8-TIMM13 complex mediates the import of proteins such as TIMM23, SLC25A12/ARALAR1 and SLC25A13/ARALAR2, while the predominant TIMM9-TIMM10 70 kDa complex mediates the import of much more proteins By similarity.By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein transport Source: UniProtKB-KW

    Keywords - Molecular functioni

    Chaperone

    Keywords - Biological processi

    Protein transport, Translocation, Transport

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_199101. Mitochondrial protein import.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitochondrial import inner membrane translocase subunit Tim8 A
    Alternative name(s):
    Deafness dystonia protein 1 homolog
    Gene namesi
    Name:Timm8a1
    Synonyms:Ddp1, Tim8a, Timm8a
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10, UP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:1353433. Timm8a1.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial inner membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 9797Mitochondrial import inner membrane translocase subunit Tim8 APRO_0000193585Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi43 ↔ 66By similarity
    Disulfide bondi47 ↔ 62By similarity
    Modified residuei87 – 871Phosphoserine1 Publication
    Modified residuei94 – 941PhosphoserineBy similarity
    Modified residuei96 – 961Phosphoserine1 Publication

    Keywords - PTMi

    Disulfide bond, Phosphoprotein

    Proteomic databases

    MaxQBiQ9WVA2.
    PaxDbiQ9WVA2.
    PRIDEiQ9WVA2.

    PTM databases

    PhosphoSiteiQ9WVA2.

    Expressioni

    Tissue specificityi

    Present at high level in liver and brain, and at lower level in muscle and heart. In CNS sections, it is predominantly present in the soma and the dendritic portion of the Purkinje cells of the cerebellum, but not in the glial cells. Scattered expression also is also detected in the brain stem, olfactory bulb, substantia nigra, hippocampus and striatum (at protein level). Ubiquitously expressed.2 Publications

    Gene expression databases

    BgeeiQ9WVA2.
    GenevestigatoriQ9WVA2.

    Interactioni

    Subunit structurei

    Heterohexamer; composed of 3 copies of TIMM8A and 3 copies of TIMM13, named soluble 70 kDa complex. Associates with the TIM22 complex, whose core is composed of TIMM22 By similarity.By similarity

    Protein-protein interaction databases

    IntActiQ9WVA2. 2 interactions.
    MINTiMINT-4137932.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9WVA2.
    SMRiQ9WVA2. Positions 29-84.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi43 – 6624Twin CX3C motifAdd
    BLAST

    Domaini

    The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space. However, during the transit of TIMM8A from cytoplasm into mitochondrion, the Cys residues probably coordinate zinc, thereby preventing folding and allowing its transfer across mitochondrial outer membrane By similarity.By similarity

    Sequence similaritiesi

    Belongs to the small Tim family.Curated

    Phylogenomic databases

    eggNOGiNOG238740.
    GeneTreeiENSGT00390000016102.
    HOGENOMiHOG000115758.
    HOVERGENiHBG060492.
    InParanoidiQ9WVA2.
    KOiK17780.
    OMAiDRSEEPC.
    OrthoDBiEOG79W97P.
    PhylomeDBiQ9WVA2.
    TreeFamiTF106191.

    Family and domain databases

    Gene3Di1.10.287.810. 1 hit.
    InterProiIPR004217. Tim10/DDP_fam_Znf.
    [Graphical view]
    PfamiPF02953. zf-Tim10_DDP. 1 hit.
    [Graphical view]
    SUPFAMiSSF144122. SSF144122. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9WVA2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MESSTSSSGS ALGAVDPQLQ HFIEVETQKQ RFQQLVHQMT ELCWEKCMDK   50
    PGPKLDSRAE ACFVNCVERF IDTSQFILNR LEQTQKSKPV FSESLSD 97
    Length:97
    Mass (Da):11,042
    Last modified:November 1, 1999 - v1
    Checksum:i16C23D7C351BDF00
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF150081 mRNA. Translation: AAD39988.1.
    AB031055 mRNA. Translation: BAA90770.1.
    AK011402 mRNA. Translation: BAB27594.1.
    AK088903 mRNA. Translation: BAC40644.1.
    AK090079 mRNA. Translation: BAC41082.1.
    AK165573 mRNA. Translation: BAE38265.1.
    BC004796 mRNA. Translation: AAH04796.1.
    BC094631 mRNA. Translation: AAH94631.1.
    CCDSiCCDS41122.1.
    PIRiJC7322.
    RefSeqiNP_038926.1. NM_013898.2.
    UniGeneiMm.214504.

    Genome annotation databases

    EnsembliENSMUST00000052902; ENSMUSP00000049749; ENSMUSG00000045455.
    ENSMUST00000054213; ENSMUSP00000050156; ENSMUSG00000048007.
    GeneIDi30058.
    KEGGimmu:30058.
    UCSCiuc009ugd.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF150081 mRNA. Translation: AAD39988.1 .
    AB031055 mRNA. Translation: BAA90770.1 .
    AK011402 mRNA. Translation: BAB27594.1 .
    AK088903 mRNA. Translation: BAC40644.1 .
    AK090079 mRNA. Translation: BAC41082.1 .
    AK165573 mRNA. Translation: BAE38265.1 .
    BC004796 mRNA. Translation: AAH04796.1 .
    BC094631 mRNA. Translation: AAH94631.1 .
    CCDSi CCDS41122.1.
    PIRi JC7322.
    RefSeqi NP_038926.1. NM_013898.2.
    UniGenei Mm.214504.

    3D structure databases

    ProteinModelPortali Q9WVA2.
    SMRi Q9WVA2. Positions 29-84.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9WVA2. 2 interactions.
    MINTi MINT-4137932.

    PTM databases

    PhosphoSitei Q9WVA2.

    Proteomic databases

    MaxQBi Q9WVA2.
    PaxDbi Q9WVA2.
    PRIDEi Q9WVA2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000052902 ; ENSMUSP00000049749 ; ENSMUSG00000045455 .
    ENSMUST00000054213 ; ENSMUSP00000050156 ; ENSMUSG00000048007 .
    GeneIDi 30058.
    KEGGi mmu:30058.
    UCSCi uc009ugd.1. mouse.

    Organism-specific databases

    CTDi 30058.
    MGIi MGI:1353433. Timm8a1.

    Phylogenomic databases

    eggNOGi NOG238740.
    GeneTreei ENSGT00390000016102.
    HOGENOMi HOG000115758.
    HOVERGENi HBG060492.
    InParanoidi Q9WVA2.
    KOi K17780.
    OMAi DRSEEPC.
    OrthoDBi EOG79W97P.
    PhylomeDBi Q9WVA2.
    TreeFami TF106191.

    Enzyme and pathway databases

    Reactomei REACT_199101. Mitochondrial protein import.

    Miscellaneous databases

    NextBioi 307190.
    PROi Q9WVA2.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9WVA2.
    Genevestigatori Q9WVA2.

    Family and domain databases

    Gene3Di 1.10.287.810. 1 hit.
    InterProi IPR004217. Tim10/DDP_fam_Znf.
    [Graphical view ]
    Pfami PF02953. zf-Tim10_DDP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF144122. SSF144122. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The mitochondrial TIM22 preprotein translocase is highly conserved throughout the eukaryotic kingdom."
      Bauer M.F., Rothbauer U., Muehlenbein N., Smith R.J.H., Gerbitz K.-D., Neupert W., Brunner M., Hofmann S.
      FEBS Lett. 464:41-47(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Cloning and expression of mouse deafness dystonia peptide 1 cDNA."
      Nakane T., Inada Y., Ito F., Itoh N., Tazawa S., Chiba S.
      Biochem. Biophys. Res. Commun. 273:759-764(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Tissue: Adipocyte.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Submandibular gland.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J and Czech II.
      Tissue: Mammary gland and Thymus.
    5. "The calcium-binding aspartate/glutamate carriers, citrin and aralar1, are new substrates for the DDP1/TIMM8a-TIMM13 complex."
      Roesch K., Hynds P.J., Varga R., Tranebjaerg L., Koehler C.M.
      Hum. Mol. Genet. 13:2101-2111(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-87 AND SER-96, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiTIM8A_MOUSE
    AccessioniPrimary (citable) accession number: Q9WVA2
    Secondary accession number(s): Q542E5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2001
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3