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Protein

Mitochondrial import inner membrane translocase subunit Tim8 A

Gene

Timm8a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mitochondrial intermembrane chaperone that participates in the import and insertion of some multi-pass transmembrane proteins into the mitochondrial inner membrane. Also required for the transfer of beta-barrel precursors from the TOM complex to the sorting and assembly machinery (SAM complex) of the outer membrane. Acts as a chaperone-like protein that protects the hydrophobic precursors from aggregation and guide them through the mitochondrial intermembrane space. The TIMM8-TIMM13 complex mediates the import of proteins such as TIMM23, SLC25A12/ARALAR1 and SLC25A13/ARALAR2, while the predominant TIMM9-TIMM10 70 kDa complex mediates the import of much more proteins (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone
Biological processProtein transport, Translocation, Transport
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit Tim8 A
Alternative name(s):
Deafness dystonia protein 1 homolog
Gene namesi
Name:Timm8a1
Synonyms:Ddp1, Tim8a, Timm8a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1353433 Timm8a1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001935851 – 97Mitochondrial import inner membrane translocase subunit Tim8 AAdd BLAST97

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi43 ↔ 66By similarity
Disulfide bondi47 ↔ 62By similarity
Modified residuei57PhosphoserineCombined sources1
Modified residuei87PhosphoserineCombined sources1
Modified residuei94PhosphoserineCombined sources1
Modified residuei96PhosphoserineCombined sources1

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

EPDiQ9WVA2
MaxQBiQ9WVA2
PaxDbiQ9WVA2
PeptideAtlasiQ9WVA2
PRIDEiQ9WVA2

PTM databases

iPTMnetiQ9WVA2
PhosphoSitePlusiQ9WVA2

Expressioni

Tissue specificityi

Present at high level in liver and brain, and at lower level in muscle and heart. In CNS sections, it is predominantly present in the soma and the dendritic portion of the Purkinje cells of the cerebellum, but not in the glial cells. Scattered expression also is also detected in the brain stem, olfactory bulb, substantia nigra, hippocampus and striatum (at protein level). Ubiquitously expressed.2 Publications

Gene expression databases

BgeeiENSMUSG00000045455
GenevisibleiQ9WVA2 MM

Interactioni

Subunit structurei

Heterohexamer; composed of 3 copies of TIMM8A and 3 copies of TIMM13, named soluble 70 kDa complex. Associates with the TIM22 complex, whose core is composed of TIMM22 (By similarity).By similarity

Protein-protein interaction databases

BioGridi205960, 1 interactor
IntActiQ9WVA2, 2 interactors
MINTiQ9WVA2
STRINGi10090.ENSMUSP00000050156

Structurei

3D structure databases

ProteinModelPortaliQ9WVA2
SMRiQ9WVA2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi43 – 66Twin CX3C motifAdd BLAST24

Domaini

The twin CX3C motif contains 4 conserved Cys residues that form 2 disulfide bonds in the mitochondrial intermembrane space. However, during the transit of TIMM8A from cytoplasm into mitochondrion, the Cys residues probably coordinate zinc, thereby preventing folding and allowing its transfer across mitochondrial outer membrane (By similarity).By similarity

Sequence similaritiesi

Belongs to the small Tim family.Curated

Phylogenomic databases

eggNOGiKOG3489 Eukaryota
ENOG41126D5 LUCA
GeneTreeiENSGT00390000016102
HOGENOMiHOG000115758
HOVERGENiHBG060492
InParanoidiQ9WVA2
KOiK17780
OMAiIESQKQR
OrthoDBiEOG091G11XE
PhylomeDBiQ9WVA2
TreeFamiTF106191

Family and domain databases

Gene3Di1.10.287.810, 1 hit
InterProiView protein in InterPro
IPR004217 Tim10-like
IPR035427 Tim10-like_dom_sf
PfamiView protein in Pfam
PF02953 zf-Tim10_DDP, 1 hit
SUPFAMiSSF144122 SSF144122, 1 hit

Sequencei

Sequence statusi: Complete.

Q9WVA2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESSTSSSGS ALGAVDPQLQ HFIEVETQKQ RFQQLVHQMT ELCWEKCMDK
60 70 80 90
PGPKLDSRAE ACFVNCVERF IDTSQFILNR LEQTQKSKPV FSESLSD
Length:97
Mass (Da):11,042
Last modified:November 1, 1999 - v1
Checksum:i16C23D7C351BDF00
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF150081 mRNA Translation: AAD39988.1
AB031055 mRNA Translation: BAA90770.1
AK011402 mRNA Translation: BAB27594.1
AK088903 mRNA Translation: BAC40644.1
AK090079 mRNA Translation: BAC41082.1
AK165573 mRNA Translation: BAE38265.1
BC004796 mRNA Translation: AAH04796.1
BC094631 mRNA Translation: AAH94631.1
CCDSiCCDS41122.1
PIRiJC7322
RefSeqiNP_038926.1, NM_013898.3
UniGeneiMm.214504

Genome annotation databases

EnsembliENSMUST00000054213; ENSMUSP00000050156; ENSMUSG00000048007
GeneIDi30058
KEGGimmu:30058
UCSCiuc009ugd.1 mouse

Similar proteinsi

Entry informationi

Entry nameiTIM8A_MOUSE
AccessioniPrimary (citable) accession number: Q9WVA2
Secondary accession number(s): Q542E5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1999
Last modified: June 20, 2018
This is version 140 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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