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Protein

Pleckstrin homology-like domain family A member 3

Gene

Phlda3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

p53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its direct transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May act as a tumor suppressor (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Apoptosis

Names & Taxonomyi

Protein namesi
Recommended name:
Pleckstrin homology-like domain family A member 3
Alternative name(s):
TDAG51/Ipl homolog 1
Gene namesi
Name:Phlda3
Synonyms:Tih1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1351485. Phlda3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are viable and fertile, and show normal placenta and embryonic weights.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi27 – 271K → A: Impairs the ability to resucue growth in cdc25ts mutant yeasts. 1 Publication
Mutagenesisi28 – 281R → A: Impairs the ability to resucue growth in cdc25ts mutant yeasts. 1 Publication

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 125125Pleckstrin homology-like domain family A member 3PRO_0000053903Add
BLAST

Proteomic databases

MaxQBiQ9WV95.
PaxDbiQ9WV95.
PRIDEiQ9WV95.

PTM databases

PhosphoSiteiQ9WV95.

Expressioni

Tissue specificityi

Widely expressed in fetal tissues, with the exception of liver. Strongly expressed in adult skeletal muscle and lung. Widely expressed at lower levels in other adult tissues, with weakest expression in liver and spleen.1 Publication

Developmental stagei

Expressed in extraembryonic tissues and placenta at 11.5 and 18.5 dpc (at protein level). Expression continues throughout gestation and is strong in adult lung (at protein level).1 Publication

Gene expression databases

BgeeiQ9WV95.
GenevisibleiQ9WV95. MM.

Interactioni

Protein-protein interaction databases

IntActiQ9WV95. 1 interaction.
MINTiMINT-8178476.

Structurei

3D structure databases

ProteinModelPortaliQ9WV95.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 125120PHAdd
BLAST

Domaini

The PH domain binds phosphoinositides with a broad specificity. It competes with the PH domain of AKT1 and directly interferes with AKT1 binding to phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3), preventing AKT1 association to membrane lipids and subsequent activation of AKT1 signaling (By similarity).By similarity

Sequence similaritiesi

Belongs to the PHLDA3 family.Curated
Contains 1 PH domain.Curated

Phylogenomic databases

eggNOGiENOG410IXIC. Eukaryota.
ENOG410YMWS. LUCA.
GeneTreeiENSGT00440000039564.
HOGENOMiHOG000115528.
HOVERGENiHBG052730.
InParanoidiQ9WV95.
OMAiVRARHSC.
OrthoDBiEOG7NKKM7.
PhylomeDBiQ9WV95.
TreeFamiTF332320.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9WV95-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAAATVLKE GVLEKRSGGL LQLWKRKRCV LTERGLQLFE AKGTGGRPKE
60 70 80 90 100
LSFARIKAVE CVESTGRHIY FTLVTEGGGE IDFRCPLEDP GWNAQITLGL
110 120
VKFKNQQAIQ TVRARQSLGT GTLVS
Length:125
Mass (Da):13,719
Last modified:November 1, 1999 - v1
Checksum:i53E44E6A29C036A4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF151099 Genomic DNA. Translation: AAD42080.1.
AK003767 mRNA. Translation: BAB22985.1.
BC023408 mRNA. Translation: AAH23408.1.
CCDSiCCDS35722.1.
RefSeqiNP_038778.1. NM_013750.2.
UniGeneiMm.34346.

Genome annotation databases

EnsembliENSMUST00000038945; ENSMUSP00000040614; ENSMUSG00000041801.
GeneIDi27280.
KEGGimmu:27280.
UCSCiuc007ctq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF151099 Genomic DNA. Translation: AAD42080.1.
AK003767 mRNA. Translation: BAB22985.1.
BC023408 mRNA. Translation: AAH23408.1.
CCDSiCCDS35722.1.
RefSeqiNP_038778.1. NM_013750.2.
UniGeneiMm.34346.

3D structure databases

ProteinModelPortaliQ9WV95.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9WV95. 1 interaction.
MINTiMINT-8178476.

PTM databases

PhosphoSiteiQ9WV95.

Proteomic databases

MaxQBiQ9WV95.
PaxDbiQ9WV95.
PRIDEiQ9WV95.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000038945; ENSMUSP00000040614; ENSMUSG00000041801.
GeneIDi27280.
KEGGimmu:27280.
UCSCiuc007ctq.2. mouse.

Organism-specific databases

CTDi23612.
MGIiMGI:1351485. Phlda3.

Phylogenomic databases

eggNOGiENOG410IXIC. Eukaryota.
ENOG410YMWS. LUCA.
GeneTreeiENSGT00440000039564.
HOGENOMiHOG000115528.
HOVERGENiHBG052730.
InParanoidiQ9WV95.
OMAiVRARHSC.
OrthoDBiEOG7NKKM7.
PhylomeDBiQ9WV95.
TreeFamiTF332320.

Miscellaneous databases

NextBioi305199.
PROiQ9WV95.
SOURCEiSearch...

Gene expression databases

BgeeiQ9WV95.
GenevisibleiQ9WV95. MM.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel pleckstrin homology-related gene family defined by Ipl/Tssc3, TDAG51, and Tih1: tissue-specific expression, chromosomal location, and parental imprinting."
    Frank D., Mendelsohn C.L., Ciccone E., Svensson K., Ohlsson R., Tycko B.
    Mamm. Genome 10:1150-1159(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  4. "Phosphoinositide binding by the pleckstrin homology domains of Ipl and Tih1."
    Saxena A., Morozov P., Frank D., Musalo R., Lemmon M.A., Skolnik E.Y., Tycko B.
    J. Biol. Chem. 277:49935-49944(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHOINOSITIDE-BINDING, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-27 AND ARG-28.
  5. Cited for: DISRUPTION PHENOTYPE.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain and Lung.

Entry informationi

Entry nameiPHLA3_MOUSE
AccessioniPrimary (citable) accession number: Q9WV95
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: November 1, 1999
Last modified: January 20, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.