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Protein

Pleckstrin homology-like domain family A member 3

Gene

Phlda3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

p53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its direct transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May act as a tumor suppressor (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processApoptosis

Names & Taxonomyi

Protein namesi
Recommended name:
Pleckstrin homology-like domain family A member 3
Alternative name(s):
TDAG51/Ipl homolog 1
Gene namesi
Name:Phlda3
Synonyms:Tih1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1351485 Phlda3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice are viable and fertile, and show normal placenta and embryonic weights.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi27K → A: Impairs the ability to resucue growth in cdc25ts mutant yeasts. 1 Publication1
Mutagenesisi28R → A: Impairs the ability to resucue growth in cdc25ts mutant yeasts. 1 Publication1

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000539031 – 125Pleckstrin homology-like domain family A member 3Add BLAST125

Proteomic databases

MaxQBiQ9WV95
PaxDbiQ9WV95
PeptideAtlasiQ9WV95
PRIDEiQ9WV95

PTM databases

iPTMnetiQ9WV95
PhosphoSitePlusiQ9WV95

Expressioni

Tissue specificityi

Widely expressed in fetal tissues, with the exception of liver. Strongly expressed in adult skeletal muscle and lung. Widely expressed at lower levels in other adult tissues, with weakest expression in liver and spleen.1 Publication

Developmental stagei

Expressed in extraembryonic tissues and placenta at 11.5 and 18.5 dpc (at protein level). Expression continues throughout gestation and is strong in adult lung (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000041801
GenevisibleiQ9WV95 MM

Interactioni

Protein-protein interaction databases

IntActiQ9WV95, 1 interactor
MINTiQ9WV95
STRINGi10090.ENSMUSP00000040614

Structurei

3D structure databases

ProteinModelPortaliQ9WV95
SMRiQ9WV95
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 125PHAdd BLAST120

Domaini

The PH domain binds phosphoinositides with a broad specificity. It competes with the PH domain of AKT1 and directly interferes with AKT1 binding to phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3), preventing AKT1 association to membrane lipids and subsequent activation of AKT1 signaling (By similarity).By similarity

Sequence similaritiesi

Belongs to the PHLDA3 family.Curated

Phylogenomic databases

eggNOGiENOG410IXIC Eukaryota
ENOG410YMWS LUCA
GeneTreeiENSGT00440000039564
HOGENOMiHOG000115528
HOVERGENiHBG052730
InParanoidiQ9WV95
OMAiWNAEIAL
OrthoDBiEOG091G0TXC
PhylomeDBiQ9WV95
TreeFamiTF332320

Family and domain databases

Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
SMARTiView protein in SMART
SM00233 PH, 1 hit

Sequencei

Sequence statusi: Complete.

Q9WV95-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAAATVLKE GVLEKRSGGL LQLWKRKRCV LTERGLQLFE AKGTGGRPKE
60 70 80 90 100
LSFARIKAVE CVESTGRHIY FTLVTEGGGE IDFRCPLEDP GWNAQITLGL
110 120
VKFKNQQAIQ TVRARQSLGT GTLVS
Length:125
Mass (Da):13,719
Last modified:November 1, 1999 - v1
Checksum:i53E44E6A29C036A4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF151099 Genomic DNA Translation: AAD42080.1
AK003767 mRNA Translation: BAB22985.1
BC023408 mRNA Translation: AAH23408.1
CCDSiCCDS35722.1
RefSeqiNP_038778.1, NM_013750.2
UniGeneiMm.34346

Genome annotation databases

EnsembliENSMUST00000038945; ENSMUSP00000040614; ENSMUSG00000041801
GeneIDi27280
KEGGimmu:27280
UCSCiuc007ctq.2 mouse

Similar proteinsi

Entry informationi

Entry nameiPHLA3_MOUSE
AccessioniPrimary (citable) accession number: Q9WV95
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: November 1, 1999
Last modified: March 28, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health