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Protein

Hairy/enhancer-of-split related with YRPW motif protein 1

Gene

Hey1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Downstream effector of Notch signaling required for cardiovascular development. Specifically required for the Notch-induced endocardial epithelial to mesenchymal transition, which is itself criticial for cardiac valve and septum development. May be required in conjunction with HEY2 to specify arterial cell fate or identity. Promotes maintenance of neuronal precursor cells and glial versus neuronal fate specification. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6 and by the neuronal bHLH factors ASCL1/MASH1 and NEUROD4/MATH3.8 Publications

GO - Molecular functioni

  • microsatellite binding Source: UniProtKB
  • protein heterodimerization activity Source: MGI
  • protein homodimerization activity Source: MGI
  • sequence-specific DNA binding Source: MGI
  • transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: UniProtKB
  • transcription factor activity, protein binding Source: UniProtKB
  • transcription factor activity, RNA polymerase II core promoter sequence-specific Source: UniProtKB
  • transcription factor activity, sequence-specific DNA binding Source: MGI

GO - Biological processi

  • anterior/posterior axis specification Source: MGI
  • arterial endothelial cell differentiation Source: BHF-UCL
  • artery development Source: MGI
  • atrioventricular valve formation Source: BHF-UCL
  • atrioventricular valve morphogenesis Source: BHF-UCL
  • blood vessel development Source: MGI
  • cardiac epithelial to mesenchymal transition Source: BHF-UCL
  • cardiac septum morphogenesis Source: BHF-UCL
  • cardiac ventricle morphogenesis Source: BHF-UCL
  • comma-shaped body morphogenesis Source: UniProtKB
  • dorsal aorta morphogenesis Source: BHF-UCL
  • endocardial cushion morphogenesis Source: BHF-UCL
  • endocardial cushion to mesenchymal transition involved in heart valve formation Source: MGI
  • epithelial to mesenchymal transition involved in endocardial cushion formation Source: MGI
  • establishment of epithelial cell polarity Source: MGI
  • inner ear receptor stereocilium organization Source: MGI
  • labyrinthine layer blood vessel development Source: BHF-UCL
  • mesenchymal cell development Source: BHF-UCL
  • negative regulation of gene expression Source: BHF-UCL
  • negative regulation of neuron differentiation Source: UniProtKB
  • negative regulation of Notch signaling pathway Source: MGI
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter involved in smooth muscle cell differentiation Source: MGI
  • negative regulation of transcription regulatory region DNA binding Source: MGI
  • Notch signaling involved in heart development Source: BHF-UCL
  • Notch signaling pathway Source: UniProtKB
  • osteoblast development Source: MGI
  • outflow tract morphogenesis Source: BHF-UCL
  • positive regulation of osteoblast differentiation Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • pulmonary valve morphogenesis Source: BHF-UCL
  • regulation of auditory receptor cell differentiation Source: MGI
  • regulation of epithelial cell proliferation Source: MGI
  • regulation of vasculogenesis Source: BHF-UCL
  • renal vesicle development Source: UniProtKB
  • S-shaped body morphogenesis Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
  • umbilical cord morphogenesis Source: BHF-UCL
  • ureteric bud morphogenesis Source: UniProtKB
  • vasculogenesis Source: MGI
  • ventricular septum morphogenesis Source: BHF-UCL
  • ventricular trabecula myocardium morphogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Notch signaling pathway, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-2122947. NOTCH1 Intracellular Domain Regulates Transcription.

Names & Taxonomyi

Protein namesi
Recommended name:
Hairy/enhancer-of-split related with YRPW motif protein 1
Alternative name(s):
Hairy and enhancer of split-related protein 1
Short name:
HESR-1
Hairy-related transcription factor 1
Short name:
HRT-1
Short name:
mHRT1
Gene namesi
Name:Hey1
Synonyms:Herp2, Hesr1, Hrt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1341800. Hey1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleoplasm Source: Reactome
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001272181 – 299Hairy/enhancer-of-split related with YRPW motif protein 1Add BLAST299

Proteomic databases

PaxDbiQ9WV93.
PRIDEiQ9WV93.

PTM databases

iPTMnetiQ9WV93.
PhosphoSitePlusiQ9WV93.

Expressioni

Tissue specificityi

Expressed in somitic mesoderm, brain, central nervous system, kidney, heart, nasal epithelium, limbs, lung, muscle, ovary and testis.2 Publications

Developmental stagei

Expressed in the developing nervous system, the somites, heart and craniofacial region. Expressed in the myocardium of the atrium at E9.5 and in the atrioventricular cushions from E9.5 to E12.5. Also expressed in developing and adult retina. Expressed in the ventricular zone of the ventral spinal cord at E12 and in the ventricular zone of the telencephalon at E12 and E15. Weakly expressed in the cortical plate at E15.5 Publications

Inductioni

By activation of the Notch signaling pathway.4 Publications

Gene expression databases

CleanExiMM_HEY1.

Interactioni

Subunit structurei

May self-associate (By similarity). Interacts with HES1, NCOR1 and SIN3A (By similarity). Interacts with GATA4, GATA6 and HDAC1 and HEYL. Interacts with CCDC89/BOIP.By similarity4 Publications

GO - Molecular functioni

  • protein heterodimerization activity Source: MGI
  • protein homodimerization activity Source: MGI

Protein-protein interaction databases

IntActiQ9WV93. 1 interactor.
STRINGi10090.ENSMUSP00000038014.

Structurei

3D structure databases

ProteinModelPortaliQ9WV93.
SMRiQ9WV93.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini49 – 104bHLHPROSITE-ProRule annotationAdd BLAST56
Domaini122 – 158OrangePROSITE-ProRule annotationAdd BLAST37

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni48 – 117Transcriptional repression and interaction with NCOR1 and SIN3ABy similarityAdd BLAST70

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi289 – 292YRPW motif4

Sequence similaritiesi

Belongs to the HEY family.Curated
Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation
Contains 1 Orange domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG4304. Eukaryota.
ENOG4111F0X. LUCA.
HOGENOMiHOG000286035.
HOVERGENiHBG003275.
InParanoidiQ9WV93.
PhylomeDBiQ9WV93.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR003650. Orange_dom.
[Graphical view]
PfamiPF07527. Hairy_orange. 1 hit.
PF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
SM00511. ORANGE. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
PS51054. ORANGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9WV93-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRAHPDYSS SDSELDETIE VEKESADENG NLSSALCSMS PTTSSQVLAR
60 70 80 90 100
KRRRGIIEKR RRDRINNSLS ELRRLVPSAF EKQGSAKLEK AEILQMTVDH
110 120 130 140 150
LKMLHTAGGK GYFDAHALAM DYRSLGFREC LAEVARYLSI IEGLDASDPL
160 170 180 190 200
LVRLVSHLNN YASQREAASG AHGGLGHIPW GSAFGHHPHI AHPLLLPQNG
210 220 230 240 250
HGNAGTAASP TEPHHQGRLA SAHPEAPALR APPSGGLGPV LPVVTSASKL
260 270 280 290
SPPLLSSVAS LSAFPFSFSS FHLLSPSTPT QAANLGKPYR PWGTEIGAF
Length:299
Mass (Da):32,063
Last modified:November 1, 1999 - v1
Checksum:i99111DD24ACEE819
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF151521 mRNA. Translation: AAD38966.1.
CCDSiCCDS17232.1.
UniGeneiMm.29581.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF151521 mRNA. Translation: AAD38966.1.
CCDSiCCDS17232.1.
UniGeneiMm.29581.

3D structure databases

ProteinModelPortaliQ9WV93.
SMRiQ9WV93.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9WV93. 1 interactor.
STRINGi10090.ENSMUSP00000038014.

PTM databases

iPTMnetiQ9WV93.
PhosphoSitePlusiQ9WV93.

Proteomic databases

PaxDbiQ9WV93.
PRIDEiQ9WV93.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:1341800. Hey1.

Phylogenomic databases

eggNOGiKOG4304. Eukaryota.
ENOG4111F0X. LUCA.
HOGENOMiHOG000286035.
HOVERGENiHBG003275.
InParanoidiQ9WV93.
PhylomeDBiQ9WV93.

Enzyme and pathway databases

ReactomeiR-MMU-2122947. NOTCH1 Intracellular Domain Regulates Transcription.

Miscellaneous databases

PROiQ9WV93.
SOURCEiSearch...

Gene expression databases

CleanExiMM_HEY1.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR003650. Orange_dom.
[Graphical view]
PfamiPF07527. Hairy_orange. 1 hit.
PF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
SM00511. ORANGE. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
PS51054. ORANGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHEY1_MOUSE
AccessioniPrimary (citable) accession number: Q9WV93
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1999
Last modified: November 2, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.